Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,990,454	C→G	17.1%	P71P (CCG→CCC)	ycaC‑III ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,990,454	0	C	G	17.1%	172.4 / 23.5	69	P71P (CCG→CCC)	ycaC‑III	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base C (29/27);  new base G (8/4);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGC  >  NC_002947/5990307‑5990596                                                                                                                                                    |                                                                                                                                               cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCg                                                                                                                                               <  1:72648/149‑1 (MQ=255) cACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCg                                                                                                                                               >  3:245317/1‑149 (MQ=255)           tCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCgg                                                                                                                                     >  5:77607/1‑149 (MQ=255)            cGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCggg                                                                                                                                    >  3:386495/1‑149 (MQ=255)                 acaacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAtt                                                                                                                               >  6:324430/1‑149 (MQ=17)                  caacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTg                                                                                                                              >  1:25163/1‑149 (MQ=255)                   aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCAttcg                                                                                                                             >  3:152974/1‑147 (MQ=17)                   aacaCCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTgg                                                                                                                             >  4:383305/1‑149 (MQ=255)                    acacCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTggg                                                                                                                            <  6:220060/149‑1 (MQ=255)                      gccggCAATGATCCGCGGCTTCTTGCCGGTCGCCTTCTCCGCCTTGACGATATCCTCGTTGTCCGAGGCGGTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTGCAGCTCGGGCACCAGCGGGCCTTTcgg                                                                                                                            <  4:295587/143‑1 (MQ=14)                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCt                                                                                                                        <  7:76632/149‑1 (MQ=17)                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCAGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCt                                                                                                                        >  8:359423/1‑149 (MQ=255)                        cTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGAGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCt                                                                                                                        >  4:325053/1‑149 (MQ=255)                            aaTGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCt                                                                                                                    >  8:320704/1‑149 (MQ=14)                                aTCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGaa                                                                                                                <  4:257665/149‑1 (MQ=255)                                   aGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCt                                                                                                             <  3:171109/149‑1 (MQ=14)                                   aGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCt                                                                                                             >  7:134456/1‑149 (MQ=14)                                      tgctTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCAGCCGGGAACATATCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCtggt                                                                                                          >  7:77892/1‑149 (MQ=14)                                         ttcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTggtggt                                                                                                       >  2:240079/1‑149 (MQ=11)                                         atcttGCCGGTCGCCTTCACCCCCTTCACGAATTCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGCCAATGTACGGCGCATCCGGGAACAGGCCTTTCAGTTCCGGCACCAGCCGGCCGTTGGTGCCTAGCTCGAAGCTggtggt                                                                                                       >  4:260478/2‑149 (MQ=17)                                          tcttGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTggaggt                                                                                                       >  5:30779/1‑148 (MQ=255)                                              gCCGGTCGCCTTCCCCGCCTTGACGAAATCCTCGTTGGCCCAGGCGTTGATCTGGCCAGGGCTGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                     <  2:153216/146‑1 (MQ=11)                                              gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGa                                                                                                     <  2:334273/146‑1 (MQ=255)                                              gCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGGACGGCGCCTCCGGGAACATCTCTTTCAGCTCGGGCACCCGCGGGCCATTCTGGCCTTGCTCGAAGCTGGGGGTGAGGa                                                                                                  >  8:368906/1‑149 (MQ=11)                                               ccGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGAt                                                                                                 <  4:386495/149‑1 (MQ=255)                                                 ggTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                                                               <  1:377355/149‑1 (MQ=255)                                                  gTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGGCACCAGCGGGCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                              >  1:316964/1‑149 (MQ=11)                                                  gTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGGTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                                                              <  6:30779/149‑1 (MQ=255)                                                     gCCTTCACCGCCTTGACGAAATCCTCGTGGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                                                                                           <  3:259737/149‑1 (MQ=255)                                                       cTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACACGCCTAACAGTTCAGGCACCAGCGGGCCGATGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCgg                                                                                           >  1:322985/1‑147 (MQ=17)                                                          cACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGt                                                                                      >  6:335820/1‑149 (MQ=255)                                                              gCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTgaa                                                                                  <  5:335820/149‑1 (MQ=255)                                                               ccTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTg                                                                                    >  7:364214/1‑146 (MQ=255)                                                                   gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAAc                                                                              <  2:25163/148‑1 (MQ=255)                                                                     cGATATCCTCGTTGGCGCTGCCGTTCATCTGGCCAGGGCTGGCACTGTACGGCGCATCCGTGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGGCTTGCTGGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGATGAACTTg                                                                           <  4:249704/149‑1 (MQ=18)                                                                      gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTgg                                                                          >  1:333474/1‑149 (MQ=255)                                                                      gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTgg                                                                          >  1:381087/1‑149 (MQ=255)                                                                      gAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCACGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTgg                                                                          >  3:175994/1‑149 (MQ=255)                                                                            cTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTTGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAgg                                                                     <  4:245317/148‑1 (MQ=255)                                                                              cGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGt                                                                    >  5:313100/1‑147 (MQ=255)                                                                               gTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTca                                                                  <  6:313100/148‑1 (MQ=255)                                                                                    cccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGCCCAGGTcagccagc                                                            >  5:31719/1‑149 (MQ=255)                                                                                     ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACtt                                                                            >  5:111913/1‑132 (MQ=255)                                                                                     ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACtt                                                                            <  6:111913/132‑1 (MQ=255)                                                                                     ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAgccagcg                                                           >  7:88732/1‑149 (MQ=255)                                                                                           gTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCa                                                     >  8:182845/1‑149 (MQ=255)                                                                                              gATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACgt                                                  >  4:141164/1‑149 (MQ=255)                                                                                               aTCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCt                                                                                                                    >  5:349300/1‑82 (MQ=255)                                                                                               aTCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCt                                                                                                                    <  6:349300/82‑1 (MQ=255)                                                                                                 cTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACgttg                                                <  7:359423/148‑1 (MQ=255)                                                                                                    gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACgttgtt                                              <  3:383305/147‑1 (MQ=255)                                                                                                    gCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCt                                            >  5:342720/1‑149 (MQ=255)                                                                                                      cAGGGCGGGCAATGTACGGCGCATCCGGGAAGCGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTGGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAAGTTGGCGAGGTCAGCCAGCGCCAGCACGTTGTTCtt                                           >  4:122731/1‑148 (MQ=17)                                                                                                       aGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGa                                         <  7:335374/149‑1 (MQ=255)                                                                                                           cgggcAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACtt                                     <  6:342720/149‑1 (MQ=255)                                                                                                             ggcAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCAAGAAcaacg                                   <  4:175994/149‑5 (MQ=255)                                                                                                              gcAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTc                                  >  1:204342/1‑149 (MQ=255)                                                                                                               cAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                                                    >  1:275495/1‑82 (MQ=255)                                                                                                               cAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                                                    <  2:275495/82‑1 (MQ=255)                                                                                                               cAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCt                                 <  2:381087/149‑1 (MQ=255)                                                                                                                aaTGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTg                                <  3:260478/149‑1 (MQ=255)                                                                                                                 aTGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTgg                               >  3:117240/1‑149 (MQ=255)                                                                                                                          gcATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATc                      <  2:333474/149‑1 (MQ=255)                                                                                                                          gcATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATc                      >  1:111085/1‑149 (MQ=255)                                                                                                                                gggAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCAc                >  1:197760/1‑149 (MQ=255)                                                                                                                                       gggCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGAc         <  3:325053/149‑1 (MQ=255)                                                                                                                                             tCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACgttg                                                <  2:248571/104‑1 (MQ=255)                                                                                                                                                gTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAgccagcgcca                                                        <  6:331208/93‑1 (MQ=255)                                                                                                                                                gTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGc  >  2:91802/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                               CACGCAAACCTCGGTCACAACACCTGCAATGATCAGCTGCTTCTTGCCGGTCGCCTTCACCGCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACAGGGCTTTCAGTTCCGGCACCAGCGGGCCGTTGGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGTTGAAGAACTTGGCCAGGTCAGCCAGCGCCAGCACGTTGTTCTTGAACTTGTCTGGCTCGATATCGCGCACCAGCGACAGCAGGC  >  NC_002947/5990307‑5990596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑