Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,027,223	A→T	7.4%	F212I (TTC→ATC) ‡	PP_5277 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,027,223	0	A	T	7.4%	167.6 / 3.3	63	F212I (TTC→ATC) ‡	PP_5277	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (27/31);  new base T (2/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GACATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGT  >  NC_002947/6027078‑6027370                                                                                                                                                  |                                                                                                                                                     gACATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGa                                                                                                                                                  <  2:338005/149‑1 (MQ=255)  aCATCTGGTCGGACAGCGAGCCGATGTGCAATAGCAGCAACGCATCACCAACCCACACGGTACTAACCATGAACAACGATATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCATAAATGTTATTATCATCGCCTCGAA‑CGAg                                                                                                                                                 <  8:258577/149‑1 (MQ=255)    atatGGTCGGCCAGCGCGCCGATGGGAAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCTGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGc                                                                                                                                               <  7:16108/149‑1 (MQ=255)     tatGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGc                                                                                                                                               >  7:138162/1‑148 (MQ=255)       tGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGaa                                                                                                                                            <  8:63973/149‑1 (MQ=255)         gTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGaa                                                                                                                                            >  5:161034/1‑147 (MQ=255)         gTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAAc                                                                                                                                           <  5:143052/148‑1 (MQ=255)         gTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATAAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGTT‑CGAGGCGatga                                                                                                                                          <  2:356531/149‑4 (MQ=255)           cGGCCAGCGCGCAGATGGGCAATTGCAGCACCGCATCACCCACCACCAAGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAgg                                                                                                                                        <  4:220036/149‑1 (MQ=255)                     gcCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGccc                                                                                                                              <  4:182854/149‑1 (MQ=255)                        gATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAgg                                                                                                                           <  8:264181/149‑1 (MQ=255)                         aTGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAgg                                                                                                                           <  8:138162/148‑1 (MQ=255)                         aTGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGGGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAGTGGCCCAgg                                                                                                                           >  8:336922/1‑148 (MQ=255)                            ggCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAAt                                                                                                                       >  5:239844/1‑149 (MQ=255)                             gCAATTGCAGCACCGCATCACCCACCACCAGGGACCACACAATGAACAAGCCAATTACGGTGATAAACCCAAGCTGCAGGCAATACCCCGCCAGAAAGTTCAGGAACAACGCCCCCAA‑CCAGCCGAACACGCCAATCCCCCAGCCAAt                                                                                                                       >  4:45370/1‑148 (MQ=255)                               aaTTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTAc                                                                                                                    >  2:76229/1‑149 (MQ=255)                                     agcaCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCaa                                                                                                              <  6:239844/149‑1 (MQ=255)                                        aCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGTT‑CGAGGCGatg                                                                                                                                           >  4:93962/1‑115 (MQ=255)                                        aCCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGTT‑CGAGGCGatg                                                                                                                                           <  3:93962/117‑3 (MQ=255)                                               cacccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCgg                                                                                                       >  3:320256/1‑146 (MQ=255)                                                acccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAg                                                                                                    >  4:79672/1‑148 (MQ=255)                                                 cccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAg                                                                                                    >  2:192013/1‑147 (MQ=255)                                                 cccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGaa                                                                                                  <  6:65878/149‑1 (MQ=255)                                                 cccaccACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGaa                                                                                                  <  6:161034/149‑1 (MQ=255)                                                  ccaaccccacGGTACTCACCATGAACAAGGCGATTTCGGGGCTGAAGCCCTGCTTCAGGCAATACACCGGCAGCAAGGTTATGATCATCGCCTCGAACCGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGaa                                                                                                  <  1:97492/143‑1 (MQ=255)                                                   caccaccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAgg                                                                                                <  3:79672/149‑1 (MQ=255)                                                    accaccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGc                                                                                               >  7:280787/1‑149 (MQ=255)                                                    accaccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGc                                                                                               <  8:280787/149‑1 (MQ=255)                                                     ccaccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAg                                                                                                                            >  5:2699/1‑119 (MQ=255)                                                     ccaccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAg                                                                                                                            <  6:2699/119‑1 (MQ=255)                                                     ccaccacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCg                                                                                              <  1:11772/149‑1 (MQ=255)                                                       accacGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCGGGCAATTACCGGCAAGCGTCGGCAGAAGGCGaa                                                                                            <  6:163515/149‑1 (MQ=255)                                                        ccacGGTACTAACCATTAACAAGGCTATTTCGGTGCTTAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGTATCATCGCCTCTAA‑CGAGGCTAACAGGGTAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAAc                                                                                           <  1:76229/149‑1 (MQ=255)                                                             gTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTc                                                                                      >  2:141674/1‑149 (MQ=255)                                                                    ccATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCTGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAg                                                                               <  3:45370/149‑1 (MQ=255)                                                                    ccATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAg                                                                               <  6:294810/149‑1 (MQ=255)                                                                    ccATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCACCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAAAGCCCCAGGCACATACCGGCAAGCGTCAGCAGCACGCGCAACCGTAGCCAAAg                                                                               >  6:321509/1‑149 (MQ=255)                                                                      aTGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGt                                                                             >  3:329/1‑149 (MQ=255)                                                                      aTGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGt                                                                             <  1:192013/149‑1 (MQ=255)                                                                                   tttCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCt                                                                >  2:94858/1‑149 (MQ=255)                                                                                       ggTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTcc                                                             <  1:115910/148‑1 (MQ=255)                                                                                        gTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGt                                                           >  3:50249/1‑149 (MQ=255)                                                                                         tGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTg                                                          >  5:276133/1‑149 (MQ=255)                                                                                          gCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGt                                                           <  1:141674/147‑1 (MQ=255)                                                                                             gAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCg                                                      >  8:27903/1‑149 (MQ=255)                                                                                                   ctgctgCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTc                                                >  1:27896/1‑149 (MQ=255)                                                                                                    tgctgcAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTcc                                               >  2:101901/1‑149 (MQ=255)                                                                                                       tgcAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTccac                                             >  2:302947/1‑148 (MQ=255)                                                                                                             cAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGccc                                      <  2:169840/149‑1 (MQ=255)                                                                                                                  cacCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCcagc                                  <  2:62584/148‑1 (MQ=255)                                                                                                                    ccGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCAcagcag                                >  4:306044/1‑148 (MQ=255)                                                                                                                       gcagcaAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAAcc                            <  4:50249/149‑1 (MQ=255)                                                                                                                       gcagcaAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAAcc                            <  4:320256/149‑1 (MQ=255)                                                                                                                        cagcaAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAATGTGACACTGCAGGCTTCCGTGAGCGGCGCTCCACCACGCCCCAGCACAACCa                           >  2:196996/1‑149 (MQ=255)                                                                                                                              ggTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcggcg                     >  4:340992/1‑149 (MQ=255)                                                                                                                                  aGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcggcgcggc                 <  6:276133/149‑1 (MQ=255)                                                                                                                                    gATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAgcggcgcggc                 >  7:198313/1‑147 (MQ=255)                                                                                                                                        atcGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagcag           >  7:29490/1‑149 (MQ=255)                                                                                                                                         tcGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCcagcag           >  5:207171/1‑148 (MQ=255)                                                                                                                                            ccTCGTT‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCg                                                      >  1:69236/1‑102 (MQ=255)                                                                                                                                            ccTCGTT‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCg                                                      <  2:69236/102‑1 (MQ=255)                                                                                                                                             cTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGc      <  5:220878/149‑1 (MQ=255)                                                                                                                                                gAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGcc     <  1:196996/147‑1 (MQ=255)                                                                                                                                                 aa‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGCACCAGCGGCGCGGCCAGCAGCAGGCCGGt  >  5:229742/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GACATATGGTCGGCCAGCGCGCCGATGGGCAATTGCAGCACCGCATCACCCACCACCACGGTACTCACCATGAACAAGGCGATTTCGGTGCTGAAGCCCTGCTGCAGGCAATACACCGGCAGCAAGGTGAGGATCATCGCCTCGAA‑CGAGGCGAACAGGGCAATGGCCCAGGCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGT  >  NC_002947/6027078‑6027370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑