Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,033,457 A→T 5.1% V32V (GTT→GTA)  PP_5284 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,033,4570AT 5.1% 235.3 / 2.7 78V32V (GTT→GTA) PP_5284hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/35);  new base T (2/2);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

GCTGGCTGTACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGCCCTGTGTTAATCTA  >  NC_002947/6033309‑6033599
                                                                                                                                                    |                                                                                                                                              
gctggctgTACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGaaaa                                                                                                                                                <  3:172017/149‑1 (MQ=255)
 ctggctgTACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGaaaaa                                                                                                                                               <  4:379209/149‑1 (MQ=255)
    gctgTACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACgg                                                                                                                                            <  3:357532/149‑1 (MQ=255)
    gctgTACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACg                                                                                                                                             <  8:319534/148‑1 (MQ=255)
     ctgTACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGACCTTCACCACCAAGCCTCGAGAAAGCTCCCCGACATTACACCCACGTACGCCTGAGCCGAGAAAAACgtc                                                                                                                                           >  4:67671/1‑147 (MQ=255)
      tgTACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGc                                                                                                                                           >  4:276193/1‑148 (MQ=255)
      tgTACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGc                                                                                                                                           >  8:10698/1‑148 (MQ=255)
        tACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCcag                                                                                                                                        <  2:85442/149‑1 (MQ=255)
        tACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCca                                                                                                                                         <  5:268405/148‑1 (MQ=255)
            tCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCagcagc                                                                                                                                    >  4:126427/1‑149 (MQ=255)
            tCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCagcagc                                                                                                                                    >  6:158316/1‑149 (MQ=255)
                 gggCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTTCGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGt                                                                                                                               <  1:51758/149‑1 (MQ=255)
                  ggCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTa                                                                                                                              <  3:276193/149‑1 (MQ=255)
                  ggCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCAAGAAAAACGGCCAGCAGCTCCGTa                                                                                                                              <  6:150229/149‑1 (MQ=255)
                     ccaacccaaGCTCGCCGACGATGGTCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCt                                                                                                                           <  4:108592/149‑1 (MQ=255)
                     ccaacccaaGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCt                                                                                                                           >  7:309254/1‑149 (MQ=255)
                       aacccaaGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGt                                                                                                                         >  4:45130/1‑149 (MQ=255)
                          ccaaGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGCGCCGAGAAATACGGCCAGCAGCTCCGTATCTGTCAg                                                                                                                      >  5:325083/1‑149 (MQ=255)
                          ccaaGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAATACGGCCAGCAGCTCCGTATCTGTCAg                                                                                                                      >  5:330444/1‑149 (MQ=255)
                          ccaaGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAg                                                                                                                      >  2:179838/1‑149 (MQ=255)
                             aGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCcagc                                                                                                                                       >  7:264177/1‑129 (MQ=255)
                             aGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCcagc                                                                                                                                       <  8:264177/129‑1 (MQ=255)
                                  gCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTg                                                                                                              >  5:337559/1‑149 (MQ=255)
                                  gCCGACGAAGGCCTCGCGGGCGGCCTCCACCAACCGGCGCAAGCTGCCGAACTTCACCACCAAGCCTCGCGAACGCTCCACGACAGTACACCCACGTACGCCTGAGCCGTGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTg                                                                                                              <  6:174612/149‑1 (MQ=255)
                                    cgacgaTGGCCTCGCGGTCGGCCTCCAGCAACCGTCGCATGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACTGCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGcc                                                                                                            <  8:107273/149‑1 (MQ=255)
                                       cgaAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCt                                                                                                         <  3:126427/149‑1 (MQ=255)
                                             ccTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGc                                                                                                   <  1:179838/149‑1 (MQ=255)
                                              cTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAGCTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGc                                                                                                   >  6:196647/1‑148 (MQ=255)
                                               tCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGcaa                                                                                                 >  1:96852/1‑149 (MQ=255)
                                               tCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGcaa                                                                                                 >  8:159062/1‑149 (MQ=255)
                                                   ggTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCaacaac                                                                                             <  6:337559/149‑1 (MQ=255)
                                                        gCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTtctc                                                                                        >  8:62274/1‑149 (MQ=255)
                                                        gCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTtctc                                                                                        <  5:158316/149‑1 (MQ=255)
                                                        gCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTtctc                                                                                        <  7:35679/149‑1 (MQ=255)
                                                          cTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCt                                                                                                               <  3:28767/124‑1 (MQ=255)
                                                          cTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCt                                                                                                               >  4:28767/1‑124 (MQ=255)
                                                          cTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTccc                                                                                      >  3:375272/1‑149 (MQ=255)
                                                          cTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTccc                                                                                      >  3:381325/1‑149 (MQ=255)
                                                           tCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTCCACCCACGTACGCCTGCGCCGAGAAAAACGGCCAGCAGCTCCGGATCTGTCAGGCTCCCTGCCCCGCGCTGCAACAACTTCcccc                                                                                      >  8:338501/1‑148 (MQ=255)
                                                             cAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTGCCTGCCCCTCGCTGCAACAACTTCTCCCt                                                                                     <  4:286100/147‑1 (MQ=255)
                                                             cAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAg                                                                                   <  7:78531/149‑1 (MQ=255)
                                                              aGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAgg                                                                                  >  7:290577/1‑149 (MQ=255)
                                                              aGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAgg                                                                                  >  6:317912/1‑149 (MQ=255)
                                                              aGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAgg                                                                                  <  2:260041/149‑1 (MQ=255)
                                                                    cGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGttc                                                                            >  6:33691/1‑149 (MQ=255)
                                                                     ggCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGttct                                                                           >  5:245010/1‑149 (MQ=255)
                                                                      gcgcAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGttctt                                                                          >  1:277122/1‑149 (MQ=255)
                                                                        gcAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTcc                                                                        >  4:136483/1‑149 (MQ=255)
                                                                            gCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTccgccg                                                                    <  7:159062/149‑1 (MQ=255)
                                                                            gCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTccgccg                                                                    <  7:62274/149‑1 (MQ=255)
                                                                               gCCGAACTTCACCAACAAGCCTCGAGTAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGc                                                                  >  1:212274/1‑148 (MQ=255)
                                                                                        cACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAATCGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCt                                                          <  8:200826/147‑1 (MQ=255)
                                                                                        cACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTg                                                         <  7:10698/148‑1 (MQ=255)
                                                                                           caacaaGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGt                                                     <  6:163138/149‑1 (MQ=255)
                                                                                            aacaaGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGtt                                                    <  6:29571/149‑1 (MQ=255)
                                                                                            aacaaGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGt                                                     >  5:29571/1‑148 (MQ=255)
                                                                                            aacaaGCCCCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGtt                                                    >  4:57058/1‑149 (MQ=255)
                                                                                                       agaAAGCTCGACGACATTACACGCAGGTACGCCTGAGCCTAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGGAACAACTTCTCCCTAGGCCGTTCTTGGGCCGGCGACTGCCTGATGTTCATTc                                               <  1:169769/143‑1 (MQ=255)
                                                                                                       agaAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCAcccc                                         <  4:381325/149‑1 (MQ=255)
                                                                                                       agaAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCAcccc                                         <  4:375272/149‑1 (MQ=255)
                                                                                                       agaAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCAGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGCCCACTCCCAGATGTTCATTCCagatc                                         >  2:169769/1‑145 (MQ=255)
                                                                                                        gaAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAATACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCAACTCCCTGATGTTCATTCCACCCCt                                        <  6:325083/149‑1 (MQ=255)
                                                                                                        gaAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAATACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCAACTCCCTGATGTTCATTCCACCCCt                                        <  6:330444/149‑1 (MQ=255)
                                                                                                             cTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCtt                                   <  2:96852/149‑1 (MQ=255)
                                                                                                               ccACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTtg                                 >  1:288071/1‑149 (MQ=255)
                                                                                                                cACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTtgc                                >  1:180656/1‑149 (MQ=255)
                                                                                                                 aCGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTtgct                               >  1:262483/1‑149 (MQ=255)
                                                                                                                   gACAATACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTCTCAGGCTCCCTGCCCCTCGCTGCAACAACATCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCATagctgc                             >  1:381339/1‑149 (MQ=255)
                                                                                                                             ccACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAAGTTCTCCCTAGGCCGTTCTTCAGCCGGCCACTCCCTGAAGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTc                   >  3:24836/1‑149 (MQ=255)
                                                                                                                                cGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGc                <  5:317912/149‑1 (MQ=255)
                                                                                                                                       cTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGACCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGCCCTgtgt         <  8:309254/149‑1 (MQ=255)
                                                                                                                                          aGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGCCCTGTGTTa       <  3:45130/148‑1 (MQ=255)
                                                                                                                                           gCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGCCCTGTGTTAAt     <  6:245010/149‑1 (MQ=255)
                                                                                                                                            ccGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGCCCTGTGTTAATc    >  2:272081/1‑149 (MQ=255)
                                                                                                                                             cGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGCCCTGTGTTAATCt   >  5:178881/1‑149 (MQ=255)
                                                                                                                                              gagaAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGCCCTGTGTTAATCTa  >  8:3936/1‑149 (MQ=255)
                                                                                                                                              gagaAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGCCCTGTGTTAATCTa  >  8:39510/1‑149 (MQ=255)
                                                                                                                                              gagaAAAACGGCCAGCAGCTACGTATCTGTCAGCCTCCCTGCCACAAGCTGAAACAACTTCTCCCTAGGCAGTACTTCCTCCAGCCACACCCAGATCTTCATTACACCCCTGCCTTTGCTCCGGACCAATTCGTCAATGTGATAAtata  >  8:91703/1‑149 (MQ=255)
                                                                                                                                               agaAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCgg                           <  8:229180/123‑1 (MQ=255)
                                                                                                                                               agaAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCgg                           >  7:229180/1‑123 (MQ=255)
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GCTGGCTGTACCTCACCGGGCCCAACCCAAGCTCGCCGACGAAGGCCTCGCGGTCGGCCTCCAGCAACCGGCGCAAGCTGCCGAACTTCACCAACAAGCCTCGAGAAAGCTCCACGACATTACACCCACGTACGCCTGAGCCGAGAAAAACGGCCAGCAGCTCCGTATCTGTCAGGCTCCCTGCCCCTCGCTGCAACAACTTCTCCCTAGGCCGTTCTTCCGCCGGCCACTCCCTGATGTTCATTCCACCCCTGCCTTTGCTGCGGCCCATTTCGGCCCTGTGTTAATCTA  >  NC_002947/6033309‑6033599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑