Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,041,137	T→C	6.3%	E14G (GAA→GGA)	PP_5293 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,041,137	0	T	C	6.3%	228.3 / 4.3	79	E14G (GAA→GGA)	PP_5293	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/40);  new base C (3/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATC  >  NC_002947/6040992‑6041281                                                                                                                                                   |                                                                                                                                                  cAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCg                                                                                                                                                 <  3:115219/148‑1 (MQ=255) cAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATATCgg                                                                                                                                                >  6:208710/1‑149 (MQ=255)    cgcCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CAt                                                                                                                                            >  3:278351/1‑149 (MQ=255)       ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTgg                                                                                                                                         >  2:381631/1‑149 (MQ=255)       ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑ATGCCGAAATCCGG‑CAATgg                                                                                                                                         <  6:38838/149‑1 (MQ=255)           ttGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCgg                                                                                                                                     >  6:183925/1‑149 (MQ=255)            tGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑ATGCCGAAATCCGG‑CAATGGGCga                                                                                                                                     <  4:387981/148‑2 (MQ=255)             gCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCggg                                                                                                                                    <  4:28972/148‑1 (MQ=255)              cccTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCgggg                                                                                                                                   <  4:355358/148‑1 (MQ=255)               ccTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGt                                                                                                                                  >  2:59240/1‑148 (MQ=255)               ccTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGt                                                                                                                                  >  4:197645/1‑148 (MQ=255)                   ggCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATc                                                                                                                              <  4:5667/148‑1 (MQ=255)                    gCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGa                                                                                                                            <  5:395214/149‑1 (MQ=255)                     cGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGAc                                                                                                                           <  3:321317/149‑1 (MQ=255)                      gTCGAAAAAAGGGGCTTGCTCGCGCTTGAGGTGCCATACCACCAGCGGGCCCACCAGGGGCCCAAGCGGCACTACCACGCCCAACAGCGCGGAGAGGGGGCACAACATCCCCCATTTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGAc                                                                                                                           <  1:52792/149‑1 (MQ=255)                       tCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAg                                                                                                                         <  3:341261/149‑1 (MQ=255)                           tAATAGGGTCTTGCTCGCGGTTGAGGTGCCATAGCACCTGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACTTCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTc                                                                                                                     <  1:87349/149‑1 (MQ=255)                            aaaaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCg                                                                                                                    <  1:27054/149‑1 (MQ=255)                            aaaaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGACGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTc                                                                                                                     >  7:89245/1‑148 (MQ=255)                             aaaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCg                                                                                                                    >  7:251752/1‑148 (MQ=255)                              aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGtt                                                                                                                      <  4:129437/145‑1 (MQ=255)                              aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGtt                                                                                                                      >  3:129437/1‑145 (MQ=255)                              aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                  >  3:355438/1‑149 (MQ=255)                              aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                  >  5:311489/1‑149 (MQ=255)                              aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                  <  8:251752/149‑1 (MQ=255)                              aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                  >  2:58874/1‑149 (MQ=255)                                gggTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATc                                                                                                                <  7:128152/149‑1 (MQ=255)                                  gTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGc                                                                                                              <  5:226297/149‑1 (MQ=255)                                       gCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGGCCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTc                                                                                                            <  1:58874/147‑1 (MQ=255)                                       gCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCa                                                                                                           <  8:26842/147‑1 (MQ=255)                                         tCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGcc                                                                                                       >  5:250669/1‑149 (MQ=255)                                              ccgctggTGCTATGGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCa                                                                                                  >  8:335065/6‑149 (MQ=255)                                                   ggTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTc                                                                                                                     <  7:79338/125‑1 (MQ=255)                                                   ggTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTc                                                                                                                     >  8:79338/1‑125 (MQ=255)                                                   ggTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcctcc                                                                                             <  6:28393/149‑1 (MQ=255)                                                       ccATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTgg                                                                                         <  6:353707/149‑1 (MQ=255)                                                          tAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCg                                                                                      <  7:28964/149‑1 (MQ=255)                                                            gCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGt                                                                                    >  3:314420/1‑149 (MQ=255)                                                             gaCCTGCGGGCCGAGCAGGTGCCCAAGCGGCACTACGAGGCCCAACAGCGCTGAGAGGTGGCATAACATCGCCCA‑TTGGCGGATTTCGG‑CATTGGGCGGGGTGAGCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGtt                                                                                   <  3:112842/148‑1 (MQ=255)                                                             cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGtt                                                                                                                      >  1:85528/1‑114 (MQ=255)                                                             cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGtt                                                                                                                      <  2:85528/114‑1 (MQ=255)                                                              aCCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCggg                                                                                                                                    <  7:318423/99‑1 (MQ=255)                                                              aCCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCggg                                                                                                                                    >  8:318423/1‑99 (MQ=255)                                                                 aGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcctcc                                                                                             <  6:245459/135‑1 (MQ=255)                                                                 aGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATGTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcctcc                                                                                             >  5:245459/1‑135 (MQ=255)                                                                  gCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                              >  8:306926/1‑149 (MQ=255)                                                                   cGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagtc                                                                             <  3:197645/149‑1 (MQ=255)                                                                           gcagGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtg                                                                     <  5:183925/149‑1 (MQ=255)                                                                               gTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtgctgc                                                                 >  1:256260/1‑149 (MQ=255)                                                                                   ccAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcctgc                                                             <  7:306926/149‑1 (MQ=255)                                                                                    cAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgct                                                            >  3:117688/1‑149 (MQ=255)                                                                                            aCTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTc                                                     >  2:180113/1‑148 (MQ=255)                                                                                               aCCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCCTGCCCCGCACCGCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaa                                                  >  7:85119/1‑148 (MQ=255)                                                                                                ccAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaaa                                                 <  5:43105/148‑1 (MQ=255)                                                                                                   ggCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAtct                                             <  1:381631/149‑1 (MQ=255)                                                                                                    gCCCAACAGCGCGGCGAGGGGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAtctc                                            <  3:305587/149‑1 (MQ=255)                                                                                                    gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAtctc                                            <  6:311489/149‑1 (MQ=255)                                                                                                           aGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCTACAGGTTCGATTCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCTGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATg                                     <  1:59240/149‑1 (MQ=255)                                                                                                                ggAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCtt                                <  2:331826/149‑1 (MQ=255)                                                                                                                      gTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                  >  2:213690/1‑61 (MQ=255)                                                                                                                      gTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                  <  1:213690/61‑1 (MQ=255)                                                                                                                      gTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTgcgc                          >  5:323489/1‑149 (MQ=255)                                                                                                                         gCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaaa                                                 >  8:349522/1‑123 (MQ=255)                                                                                                                           aGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGGCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAg                     >  1:323200/1‑149 (MQ=255)                                                                                                                           aGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGATAATCTCGTTCATGCCCTTCTGCGCCAAAg                     >  7:105175/1‑149 (MQ=255)                                                                                                                            gAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGc                    <  6:323489/149‑1 (MQ=255)                                                                                                                               cATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAg                 >  5:115535/1‑149 (MQ=255)                                                                                                                               cATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGGTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAg                 <  5:264169/149‑1 (MQ=255)                                                                                                                                cccGCCCA‑ATGCCGAAATCCGG‑CAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATCCCCCGCACCTCCTTCGCCGGTTTCACTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCCTTCATGCCCTTCTGCGCCAAAGCGAg                 >  3:312342/3‑148 (MQ=255)                                                                                                                                aTCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGc                <  2:323200/149‑1 (MQ=255)                                                                                                                                   gCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATg             >  2:26461/1‑149 (MQ=255)                                                                                                                                   gCCCA‑ATGCCGAAATCCGG‑CAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATg             >  1:381698/1‑149 (MQ=255)                                                                                                                                    cccA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATgg            <  5:208710/149‑1 (MQ=255)                                                                                                                                       caatGCCGAAATCCGG‑CAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGGCATTCTGCGCCAAAGCGATCATTGCg          >  8:343669/4‑149 (MQ=255)                                                                                                                                         ttGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGtt        <  4:117688/149‑1 (MQ=255)                                                                                                                                            ccGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGa      >  3:244291/1‑148 (MQ=255)                                                                                                                                             cGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGaa     >  2:276814/1‑148 (MQ=255)                                                                                                                                              ggATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGACAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAAt   <  8:89245/149‑1 (MQ=255)                                                                                                                                               gATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATc  >  6:98487/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CAGCGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATC  >  NC_002947/6040992‑6041281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑