Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,041,143	T→A	6.4%	N12I (AAT→ATT)	PP_5293 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,041,143	0	T	A	6.4%	236.2 / 5.1	78	N12I (AAT→ATT)	PP_5293	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/40);  new base A (3/2);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

CGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGC  >  NC_002947/6040995‑6041288                                                                                                                                                       |                                                                                                                                                  cgcCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CAt                                                                                                                                                   >  3:278351/1‑149 (MQ=255)    ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTgg                                                                                                                                                >  2:381631/1‑149 (MQ=255)    ctctTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑ATGCCGAAATCCGG‑CAATgg                                                                                                                                                <  6:38838/149‑1 (MQ=255)        ttGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCgg                                                                                                                                            >  6:183925/1‑149 (MQ=255)         tGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑ATGCCGAAATCCGG‑CAATGGGCga                                                                                                                                            <  4:387981/148‑2 (MQ=255)          gCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCggg                                                                                                                                           <  4:28972/148‑1 (MQ=255)           cccTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCgggg                                                                                                                                          <  4:355358/148‑1 (MQ=255)            ccTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGt                                                                                                                                         >  2:59240/1‑148 (MQ=255)            ccTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGt                                                                                                                                         >  4:197645/1‑148 (MQ=255)                ggCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATc                                                                                                                                     <  4:5667/148‑1 (MQ=255)                 gCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGa                                                                                                                                   <  5:395214/149‑1 (MQ=255)                  cGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGAc                                                                                                                                  <  3:321317/149‑1 (MQ=255)                   gTCGAAAAAAGGGGCTTGCTCGCGCTTGAGGTGCCATACCACCAGCGGGCCCACCAGGGGCCCAAGCGGCACTACCACGCCCAACAGCGCGGAGAGGGGGCACAACATCCCCCATTTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGAc                                                                                                                                  <  1:52792/149‑1 (MQ=255)                    tCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAg                                                                                                                                <  3:341261/149‑1 (MQ=255)                        tAATAGGGTCTTGCTCGCGGTTGAGGTGCCATAGCACCTGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACTTCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTc                                                                                                                            <  1:87349/149‑1 (MQ=255)                         aaaaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCg                                                                                                                           <  1:27054/149‑1 (MQ=255)                         aaaaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGACGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTc                                                                                                                            >  7:89245/1‑148 (MQ=255)                          aaaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCg                                                                                                                           >  7:251752/1‑148 (MQ=255)                           aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGtt                                                                                                                             >  3:129437/1‑145 (MQ=255)                           aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGtt                                                                                                                             <  4:129437/145‑1 (MQ=255)                           aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                         >  2:58874/1‑149 (MQ=255)                           aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                         <  8:251752/149‑1 (MQ=255)                           aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                         >  5:311489/1‑149 (MQ=255)                           aaGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                         >  3:355438/1‑149 (MQ=255)                             gggTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATc                                                                                                                       <  7:128152/149‑1 (MQ=255)                               gTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGc                                                                                                                     <  5:226297/149‑1 (MQ=255)                                    gCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGGCCATTGGGCGGGGTGATCGACAGGTTCGAATCGCTc                                                                                                                   <  1:58874/147‑1 (MQ=255)                                    gCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCa                                                                                                                  <  8:26842/147‑1 (MQ=255)                                      tCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGcc                                                                                                              >  5:250669/1‑149 (MQ=255)                                           ccgctggTGCTATGGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCa                                                                                                         >  8:335065/6‑149 (MQ=255)                                                ggTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTc                                                                                                                            >  8:79338/1‑125 (MQ=255)                                                ggTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTc                                                                                                                            <  7:79338/125‑1 (MQ=255)                                                ggTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcctcc                                                                                                    <  6:28393/149‑1 (MQ=255)                                                    ccATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTgg                                                                                                <  6:353707/149‑1 (MQ=255)                                                       tAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCg                                                                                             <  7:28964/149‑1 (MQ=255)                                                         gCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGt                                                                                           >  3:314420/1‑149 (MQ=255)                                                          gaCCTGCGGGCCGAGCAGGTGCCCAAGCGGCACTACGAGGCCCAACAGCGCTGAGAGGTGGCATAACATCGCCCA‑TTGGCGGATTTCGG‑CATTGGGCGGGGTGAGCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGtt                                                                                          <  3:112842/148‑1 (MQ=255)                                                          cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGtt                                                                                                                             <  2:85528/114‑1 (MQ=255)                                                          cACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGtt                                                                                                                             >  1:85528/1‑114 (MQ=255)                                                           aCCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCggg                                                                                                                                           >  8:318423/1‑99 (MQ=255)                                                           aCCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCggg                                                                                                                                           <  7:318423/99‑1 (MQ=255)                                                              aGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcctcc                                                                                                    <  6:245459/135‑1 (MQ=255)                                                              aGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATGTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCAcctcc                                                                                                    >  5:245459/1‑135 (MQ=255)                                                               gCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagt                                                                                     >  8:306926/1‑149 (MQ=255)                                                                cGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTtcagtc                                                                                    <  3:197645/149‑1 (MQ=255)                                                                        gcagGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtg                                                                            <  5:183925/149‑1 (MQ=255)                                                                            gTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGtgctgc                                                                        >  1:256260/1‑149 (MQ=255)                                                                                ccAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGctgcctgc                                                                    <  7:306926/149‑1 (MQ=255)                                                                                 cAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCctgct                                                                   >  3:117688/1‑149 (MQ=255)                                                                                         aCTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTc                                                            >  2:180113/1‑148 (MQ=255)                                                                                            aCCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCCTGCCCCGCACCGCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaa                                                         >  7:85119/1‑148 (MQ=255)                                                                                             ccAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaaa                                                        <  5:43105/148‑1 (MQ=255)                                                                                                ggCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAtct                                                    <  1:381631/149‑1 (MQ=255)                                                                                                 gCCCAACAGCGCGGCGAGGGGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAtctc                                                   <  3:305587/149‑1 (MQ=255)                                                                                                 gCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAAtctc                                                   <  6:311489/149‑1 (MQ=255)                                                                                                        aGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCTACAGGTTCGATTCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCTGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATg                                            <  1:59240/149‑1 (MQ=255)                                                                                                             ggAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCtt                                       <  2:331826/149‑1 (MQ=255)                                                                                                                   gTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                         <  1:213690/61‑1 (MQ=255)                                                                                                                   gTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGaa                                                                                                                         >  2:213690/1‑61 (MQ=255)                                                                                                                   gTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTgcgc                                 >  5:323489/1‑149 (MQ=255)                                                                                                                      gCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGaaa                                                        >  8:349522/1‑123 (MQ=255)                                                                                                                        aGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGGCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAg                            >  1:323200/1‑149 (MQ=255)                                                                                                                        aGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGATAATCTCGTTCATGCCCTTCTGCGCCAAAg                            >  7:105175/1‑149 (MQ=255)                                                                                                                         gAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGc                           <  6:323489/149‑1 (MQ=255)                                                                                                                            cATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAg                        >  5:115535/1‑149 (MQ=255)                                                                                                                            cATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGGTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAg                        <  5:264169/149‑1 (MQ=255)                                                                                                                             cccGCCCA‑ATGCCGAAATCCGG‑CAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATCCCCCGCACCTCCTTCGCCGGTTTCACTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCCTTCATGCCCTTCTGCGCCAAAGCGAg                        >  3:312342/3‑148 (MQ=255)                                                                                                                             aTCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGc                       <  2:323200/149‑1 (MQ=255)                                                                                                                                gCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATg                    >  2:26461/1‑149 (MQ=255)                                                                                                                                gCCCA‑ATGCCGAAATCCGG‑CAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATg                    >  1:381698/1‑149 (MQ=255)                                                                                                                                 cccA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATgg                   <  5:208710/149‑1 (MQ=255)                                                                                                                                    caatGCCGAAATCCGG‑CAATGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGGCATTCTGCGCCAAAGCGATCATTGCg                 >  8:343669/4‑149 (MQ=255)                                                                                                                                      ttGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGtt               <  4:117688/149‑1 (MQ=255)                                                                                                                                         ccGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGa             >  3:244291/1‑148 (MQ=255)                                                                                                                                          cGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGaa            >  2:276814/1‑148 (MQ=255)                                                                                                                                           ggATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGACAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAAt          <  8:89245/149‑1 (MQ=255)                                                                                                                                            gATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATc         >  6:98487/1‑149 (MQ=255)                                                                                                                                                 cGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTcc     <  4:355438/148‑1 (MQ=255)                                                                                                                                                   g‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCTTGGCGTTGAAATCTTCCGGc  >  4:240565/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                  CGCCTCTTTGCCCTGGGCGTCGATAAAAGGGTCTTGCTCGCGCTTGAGGTGCCATAGCACCAGCGGGCCCAGCAGGTGCCCAAGCGGCACTACCAGGCCCAACAGCGCGGAGAGGTGGCAGAACATCGCCCA‑TTGCCGGATTTCGG‑CATTGGGCGGGGTGATCGACAGGTTCGAATCGCTCATGCCCCGCACCTCCTTGGCCGGTTTCAGTCAGCCAGTGCTGCCTGCTGCAGCTCGAAAATCTCGTTCATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGC  >  NC_002947/6040995‑6041288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑