Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,052,396	A→C	5.3%	E230A (GAG→GCG)	PP_5304 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,052,396	0	A	C	5.3%	269.6 / 3.6	87	E230A (GAG→GCG)	PP_5304	lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (45/37);  new base C (3/2);  total (48/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCGACCATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCTCCCACAGGGA  >  NC_002947/6052258‑6052544                                                                                                                                            |                                                                                                                                                     gcgcGACCATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGc                                                                                                                                             <  3:362547/148‑1 (MQ=255) gcgcGACCATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCg                                                                                                                                            >  7:300747/1‑149 (MQ=255)  cgcgACCATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGGGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGCTCTGGCatttc                                                                                                                                           <  5:263714/149‑5 (MQ=255)    cgACCATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGc                                                                                                                                         <  6:71338/149‑1 (MQ=255)      aCCATGGGTGGCAACAATGACAGGCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCac                                                                                                                                       >  8:345830/1‑148 (MQ=255)      aCCATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGc                                                                                                                                         >  2:66184/1‑147 (MQ=255)       ccATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAg                                                                                                                                      >  1:152962/1‑149 (MQ=255)       ccATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAg                                                                                                                                      >  8:147320/1‑149 (MQ=255)       ccATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAg                                                                                                                                      >  4:280390/1‑149 (MQ=255)        cATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGc                                                                                                                                     >  8:70972/1‑149 (MQ=255)         aTGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCCCCGTTCATCAT‑CAAGCGCGATGTTGACAAGATGGAGATTTTCCAGGCAGTGAACCGTGCACCGCCGGATGAAATGCCAGCGCTGGCAGCGGGCAAGCg                                                                                                                                    >  6:368352/1‑149 (MQ=255)          tGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGt                                                                                                                                   >  3:199850/1‑149 (MQ=255)            ggTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTTCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCa                                                                                                                                 <  7:17884/149‑1 (MQ=255)             gTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAg                                                                                                                                <  4:9486/149‑1 (MQ=255)              tGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCGGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGt                                                                                                                               <  4:316304/149‑1 (MQ=255)              tGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGt                                                                                                                               <  6:203252/149‑1 (MQ=255)                gCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTg                                                                                                                              >  2:196787/1‑148 (MQ=255)                 caacaaTGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGAGGGCAAGCGTCAGTGGa                                                                                                                            >  7:164047/1‑149 (MQ=255)                     aaTGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCt                                                                                                                        >  2:235813/1‑149 (MQ=255)                     aaTGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCCTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCt                                                                                                                        <  5:336796/149‑1 (MQ=255)                      aTGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCTGGCAAGCGTCAGTGGACGCTg                                                                                                                       >  8:221489/1‑149 (MQ=255)                      aTGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTg                                                                                                                       <  3:280390/149‑1 (MQ=255)                      aTGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTg                                                                                                                       <  2:152962/149‑1 (MQ=255)                       tGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCTGTGAACCGTGAACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTg                                                                                                                       >  1:359924/1‑148 (MQ=255)                         aCAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGa                                                                                                                     <  4:183428/148‑1 (MQ=255)                            gCCTGTGGCTGGAGCGCAACCAGCGTGGTACACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAgcgc                                                                                                                 <  3:211003/149‑1 (MQ=255)                             ccTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCa                                                                                                                >  8:53434/1‑149 (MQ=255)                              cTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAg                                                                                                               <  8:300747/149‑1 (MQ=255)                                gtgGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCt                                                                                                             >  3:118262/1‑149 (MQ=255)                                 tgGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCATCCAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGc                                                                                                              <  1:132249/148‑1 (MQ=255)                                          cgcAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATgc                                                                                                   >  3:5213/1‑149 (MQ=255)                                             aaCCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGcgcg                                                                                                >  7:278620/1‑149 (MQ=255)                                               ccAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGt                                                                                              >  6:368181/1‑149 (MQ=255)                                                cAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTc                                                                                             >  6:397850/1‑149 (MQ=255)                                                      ggtgCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTa                                                                                       <  7:53434/149‑1 (MQ=255)                                                       gtgCACCGTTGATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTa                                                                                       <  2:324129/148‑1 (MQ=255)                                                        tgCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAg                                                                                      >  2:325018/1‑148 (MQ=255)                                                          cACCGTTCATCAT‑CAAGCGCGATGGTGACAAGATGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCAAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCATGTAGGAt                                                                                   >  7:58947/1‑149 (MQ=255)                                                             cGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCgg                                                                                <  6:206584/149‑1 (MQ=255)                                                              gTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCggg                                                                               <  2:200177/149‑1 (MQ=255)                                                                  atcat‑caAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAATGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTa                                                                           >  8:22214/1‑149 (MQ=255)                                                                     at‑caAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTAccc                                                                        >  1:1902/1‑149 (MQ=255)                                                                      t‑caAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCcg                                                                       >  8:107275/1‑149 (MQ=255)                                                                        caAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCcgc                                                                      >  3:17241/1‑149 (MQ=255)                                                                         aaGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCcgc                                                                      <  7:107275/148‑1 (MQ=255)                                                                          aGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCcgcg                                                                     >  8:332778/1‑148 (MQ=255)                                                                           gcgcGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGcgcg                                                                                                >  8:211287/1‑120 (MQ=255)                                                                           gcgcGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGcgcg                                                                                                <  7:211287/120‑1 (MQ=255)                                                                           gcgcGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGAGCGGTCCCTGTAGTATCGGGTTTACCCGCgaa                                                                   >  5:193675/1‑149 (MQ=255)                                                                            cgcgATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCgaag                                                                  <  8:164047/149‑1 (MQ=255)                                                                                   gTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAATCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGaca                                                            <  6:193675/148‑1 (MQ=255)                                                                                            tGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGa                                                  >  5:273727/1‑149 (MQ=255)                                                                                              gagaTTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGAt                                                 <  5:397850/148‑1 (MQ=255)                                                                                              gagaTTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGAt                                                 <  1:66184/148‑1 (MQ=255)                                                                                              gagaTTTTCCAGGCAATGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATg                                                <  7:22214/149‑1 (MQ=255)                                                                                               agaTGTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGATGGCAGCGGGCAAGCGTCAGTGGACGCGGGAGCGCAGCTGAGATGATGCGCGGTCCCTTTAGGATCGGTTTTACCCGCGAAGAAGACACCGCGGTGGATg                                                <  5:368352/148‑1 (MQ=39)                                                                                                gaTTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATgg                                               >  5:202518/1‑148 (MQ=255)                                                                                                gaTTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGc                                              <  6:202518/149‑1 (MQ=255)                                                                                                     tCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACgg                                          <  7:70972/148‑1 (MQ=255)                                                                                                     tCCAGGCAGTGAAACGTGCACAGCCGGATGAAATGCCAGAGGTGGCAGCTGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGGGCGGTCCCTGTAGGATCGGGTTTACCCGCGAATACGACACCGCGGTGGATTTCACgg                                          <  8:278620/148‑1 (MQ=255)                                                                                                      ccAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCgaaga                                                                 >  8:242364/1‑124 (MQ=255)                                                                                                       cAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCt                                       >  8:218038/1‑149 (MQ=255)                                                                                                        aGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGAGGATGGCACGTGCtt                                      >  6:272924/1‑149 (MQ=255)                                                                                                          gCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCtttg                                    >  7:209511/1‑147 (MQ=255)                                                                                                          gCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCg                                    >  5:166007/1‑149 (MQ=255)                                                                                                             gTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGccc                                 <  5:368181/149‑1 (MQ=255)                                                                                                              tGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCg                                >  6:350666/1‑149 (MQ=255)                                                                                                              tGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCg                                <  5:305540/149‑1 (MQ=255)                                                                                                              tGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGAAGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCg                                >  6:167853/1‑149 (MQ=255)                                                                                                               gAACCCGGCACAGCCGGATGAAACGCCAGAGCTGCCAGCGGGCAAGCGTCCGTGGACGCTGGAGCGCAGCTGAGATGATGCGCCGTCCCTGTCGGATCGGGCTTACCCGCGAAGAAGACACCGCGGTGGAGGGCACGGGCTTCGCCCgt                               <  4:199850/149‑1 (MQ=37)                                                                                                                aaCCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCttg                              >  4:204712/1‑149 (MQ=255)                                                                                                                  ccGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTg                                                    <  1:338571/125‑1 (MQ=255)                                                                                                                  ccGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTg                                                    >  2:338571/1‑125 (MQ=255)                                                                                                                     tGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTcgc                         >  8:155708/1‑149 (MQ=255)                                                                                                                        acaGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCggg                      <  4:118262/149‑1 (MQ=255)                                                                                                                             cGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGc                 <  6:166007/149‑1 (MQ=255)                                                                                                                              ggATGAAATGCCGGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGcc                >  4:212231/1‑149 (MQ=255)                                                                                                                              ggATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGcc                >  1:344347/1‑149 (MQ=255)                                                                                                                                  gAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCt            >  5:349142/1‑149 (MQ=255)                                                                                                                                  ccgctGCCAGCTCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCt            >  1:88094/5‑149 (MQ=255)                                                                                                                                    aaTGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCTcc          <  4:5213/149‑1 (MQ=255)                                                                                                                                     ctGCCAGCTCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCgg                       >  6:360742/2‑135 (MQ=255)                                                                                                                                     ctGCCAGCTCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCgg                       <  5:360742/134‑1 (MQ=255)                                                                                                                                      tGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCTCCca        <  5:246010/149‑1 (MQ=255)                                                                                                                                        ccAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGc                                   <  6:71207/120‑1 (MQ=255)                                                                                                                                        ccAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGc                                   >  5:71207/1‑120 (MQ=255)                                                                                                                                        ccAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCTCCca        >  5:373656/1‑147 (MQ=255)                                                                                                                                          ggagCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCTCCCACAgg    <  3:212231/148‑1 (MQ=255)                                                                                                                                          agagCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGTAGCAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCTCCCACAgg    <  5:272924/149‑1 (MQ=255)                                                                                                                                            agCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCTCCCACAGGGa  <  7:332778/149‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                     GCGCGACCATGGGTGGCAACAATGACAGCCTGTGGCTGGAGCGCAACCAGCGTGGTGCACCGTTCATCAT‑CAAGCGCGATGGTGACAAGCTGGAGATTTTCCAGGCAGTGAACCGTGCACAGCCGGATGAAATGCCAGAGCTGGCAGCGGGCAAGCGTCAGTGGACGCTGGAGCGCAGCTGAGATGATGCGCGGTCCCTGTAGGATCGGGTTTACCCGCGAAGAAGACACCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCAAGCCCGCTCCCACAGGGA  >  NC_002947/6052258‑6052544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑