Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,056,171 A→G 10.1% T100A (ACC→GCC)  oxyR → oxidative and nitrosative stress transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,056,1710AG10.1% 182.3 / 9.9 70T100A (ACC→GCC) oxyRoxidative and nitrosative stress transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base A (30/32);  new base G (4/3);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

CAGCAAAAGCGCAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTG  >  NC_002947/6056023‑6056317
                                                                                                                                                    |                                                                                                                                                   
cagcaAAAGCGCAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCTGGCAAGAATCATCTGACCTCTCCGATCAAGGTCTGCGCCATCTaca                                                                                                                                                     <  8:174441/149‑1 (MQ=255)
 agcaAAAGCGCAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTacac                                                                                                                                                    >  2:48841/1‑149 (MQ=255)
   caAAAGCGCAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCg                                                                                                                                                  >  8:51470/1‑149 (MQ=255)
   caAAAGCGCAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTAATGGAGCAGGCCCAGGCCAGTCGCGAGCTGGCCCAGGCCCGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGAGACATCTACAccc                                                                                                                                                  >  2:297342/1‑148 (MQ=255)
    aaaaGCGCAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACGCCGt                                                                                                                                                 >  5:344792/1‑149 (MQ=255)
    aaaaGCGCAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGt                                                                                                                                                 >  6:342821/1‑149 (MQ=255)
     aaaGCGCAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTc                                                                                                                                                >  5:314907/1‑149 (MQ=255)
        gcgcAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCTAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGc                                                                                                                                             <  7:139307/149‑1 (MQ=255)
        gcgcAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGc                                                                                                                                             >  6:216581/1‑149 (MQ=255)
             gTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCAATCTACACCGTCGGCCCCTa                                                                                                                                        >  6:148125/1‑149 (MQ=255)
              tACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTAc                                                                                                                                       <  7:51470/149‑1 (MQ=255)
              tACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTAc                                                                                                                                       <  6:314907/149‑1 (MQ=255)
                 gCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTAc                                                                                                                                       <  5:230481/146‑1 (MQ=255)
                 gCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTAc                                                                                                                                       >  6:230481/1‑146 (MQ=255)
                   cTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTAATGGAGCAGGCCCAGGGAATTCGCGAGCTGGCCAAGGCCGGCAAGCATCAGCTGACCGCTCCGCTAAAGGTCGGCGCCATCTACCCCGTCGGGCCCTACCTGtt                                                                                                                                  >  8:163395/1‑149 (MQ=255)
                        cccGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGc                                                                                                                             >  2:272967/1‑149 (MQ=255)
                          cGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCAc                                                                                                                           <  5:169853/149‑1 (MQ=255)
                          cGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCAc                                                                                                                           >  5:135495/1‑149 (MQ=255)
                          cGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCAc                                                                                                                           >  5:86742/1‑149 (MQ=255)
                          cGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCAc                                                                                                                           <  3:334548/149‑1 (MQ=255)
                              cGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGa                                                                                                                       >  4:197816/1‑149 (MQ=255)
                                 cGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATcc                                                                                                                    <  3:197816/149‑1 (MQ=255)
                                     aaCATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCTCTCAAGGTCTGCGACATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCa                                                                                                                <  2:242232/149‑1 (MQ=255)
                                        aTCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCt                                                                                                             <  6:135495/149‑1 (MQ=255)
                                        aTCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCt                                                                                                             <  6:86742/149‑1 (MQ=255)
                                         tcgtcgCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACGCCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTg                                                                                                            >  3:143835/1‑149 (MQ=255)
                                                ccAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACcgcg                                                                                                     >  3:178670/1‑149 (MQ=255)
                                                 cAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCATCTTATATATGAGATGAAGTTCGGCGACATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGt                                                                                                    <  4:364038/149‑1 (MQ=255)
                                                 cAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGt                                                                                                    >  7:150471/1‑149 (MQ=255)
                                                    gcgcAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGgc                                                                                                 >  2:182063/1‑149 (MQ=255)
                                                          aatgTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCGAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGcgccgc                                                                                            <  3:248852/145‑1 (MQ=255)
                                                          aaaGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTAGGCCCCTACCTGTTCCCCCCCCAGATACCGCAGCTGCCCCCCCTGGcgccgc                                                                                            >  4:248852/1‑148 (MQ=255)
                                                           aaGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCa                                                                                           >  3:166715/1‑148 (MQ=255)
                                                               aCTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCAGCTCAAGGTCGGCGCCATCTACACCGTCGGCAGCTACCTGTTCCCGCACCTGATCCAGCAGCTGCACCGCGTGGCGCCGCAAATGc                                                                                      >  5:38201/1‑149 (MQ=255)
                                                                  ggAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCGACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCCCAAATCCCGc                                                                                   >  5:382434/1‑149 (MQ=255)
                                                                   gAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCt                                                                                  <  3:327973/149‑1 (MQ=255)
                                                                   gAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAAGCAGCTGACCGCGCCGCTCAAGGGCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCg                                                                                    <  7:251993/147‑1 (MQ=255)
                                                                     gCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAATGTCGGCGCCATCTACGCCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTg                                                                                 >  1:28051/1‑148 (MQ=255)
                                                                     gCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGGTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTg                                                                                 >  5:150507/1‑148 (MQ=255)
                                                                      cAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGATCAAGGTCGGCGACATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTa                                                                               <  4:382579/149‑1 (MQ=255)
                                                                        ggCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACa                                                                             <  5:363770/149‑1 (MQ=255)
                                                                           ccAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATc                                                                           >  3:258860/1‑148 (MQ=255)
                                                                            cAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCga                                                                         <  1:48841/149‑1 (MQ=255)
                                                                             aGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCTCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCgaa                                                                        <  7:163395/149‑1 (MQ=255)
                                                                             aGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCgaa                                                                        <  4:37574/149‑1 (MQ=255)
                                                                              gggCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCg                                                                          >  7:220979/1‑146 (MQ=255)
                                                                               ggCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATACCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCgaag                                                                       >  7:127060/1‑148 (MQ=255)
                                                                                  aTTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGaaaa                                                                   <  5:216581/149‑1 (MQ=255)
                                                                                      gcgAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTc                                                               <  5:342821/149‑1 (MQ=255)
                                                                                       cgAGCTGGCCCAGGCCTGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCa                                                              <  1:297342/149‑1 (MQ=255)
                                                                                       cgAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCa                                                              >  3:107212/1‑149 (MQ=255)
                                                                                          gCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACGCCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑ccc                                                          <  6:344792/149‑1 (MQ=255)
                                                                                           cTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCa                                                         <  8:220979/149‑1 (MQ=255)
                                                                                                ccAGGCCGGCAAGATTCAGCTGACCGCTGAGCTCAAGGTCGGAGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTTCACCGCGTGGCGCCGCAAATTCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTa                                                     <  6:38201/148‑1 (MQ=255)
                                                                                                     ccGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGt                                               <  6:150507/149‑1 (MQ=255)
                                                                                                         cAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGaaa                                           <  3:195050/149‑1 (MQ=255)
                                                                                                               tCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGc                                                 >  4:169743/1‑137 (MQ=255)
                                                                                                               tCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGc                                                 <  3:169743/137‑1 (MQ=255)
                                                                                                               tCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTgc                                     >  6:153411/1‑149 (MQ=255)
                                                                                                                 aGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGt                                               >  5:200826/1‑137 (MQ=255)
                                                                                                                 aGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGt                                               <  6:200826/137‑1 (MQ=255)
                                                                                                                        cgctccgctcAAGGTCGGCGCCATCTACACCGTCGGCCACTTCCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGc                             >  6:126016/1‑148 (MQ=255)
                                                                                                                             cgctcAATGTCGGCGCCATCTACGCCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACt                        <  2:28051/148‑1 (MQ=255)
                                                                                                                                tcAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGAc                    >  6:28079/1‑149 (MQ=255)
                                                                                                                                   aGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGAcgcg                 <  1:182063/149‑1 (MQ=255)
                                                                                                                                      tCGGCGCCATCTACGCCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGAc                    <  8:65426/143‑1 (MQ=255)
                                                                                                                                      tCGGCGCCATCTACGCCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGAc                    >  7:65426/1‑143 (MQ=255)
                                                                                                                                      tCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCACCCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGt               <  1:272967/149‑1 (MQ=255)
                                                                                                                                         gcgcCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGatc           <  4:166715/149‑1 (MQ=255)
                                                                                                                                            ccATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGatcatc        <  7:361229/149‑1 (MQ=255)
                                                                                                                                              aTCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGc      >  2:213715/1‑149 (MQ=255)
                                                                                                                                               tCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGcc     >  5:399531/1‑149 (MQ=255)
                                                                                                                                                  acacCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGAGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCCGTGATCATCGCCCTg  >  7:232950/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGCAAAAGCGCAGTACGCCTGACCCCGGTCGGCGAAAACATCGTCGCCCAAGCGCAGAAAGTACTGGAGCAGGCCCAGGGCATTCGCGAGCTGGCCCAGGCCGGCAAGAATCAGCTGACCGCTCCGCTCAAGGTCGGCGCCATCTACACCGTCGGCCCCTACCTGTTCCCGCACCTGATCCCGCAGCTGCACCGCGTGGCGCCGCAAATGCCGCTGTACATCGAAGAAAACTTCA‑CCCACGTACTGCGTGAAAAGCTGCGCAACGGCGAACTGGACGCGGTGATCATCGCCCTG  >  NC_002947/6056023‑6056317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑