Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,128,893	G→T	5.3%	G30W (GGG→TGG)	PP_5376 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,128,893	0	G	T	5.3%	240.9 / 3.5	75	G30W (GGG→TGG)	PP_5376	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (39/32);  new base T (2/2);  total (41/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

TCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGT  >  NC_002947/6128746‑6129039                                                                                                                                                    |                                                                                                                                                    tCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACg                                                                                                                                                     <  3:249727/148‑1 (MQ=255)  cACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACgg                                                                                                                                                    >  5:162341/1‑148 (MQ=255)      ggggAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAga                                                                                                                                               <  1:354089/149‑1 (MQ=255)            aCAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTATCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTACTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGAtt                                                                                                                                          >  4:383141/1‑148 (MQ=255)               ggTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAgg                                                                                                                                      <  6:139050/149‑1 (MQ=255)                    tgcCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAAt                                                                                                                                 >  6:111068/1‑149 (MQ=255)                     gcCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAAt                                                                                                                                 >  7:248837/1‑148 (MQ=255)                       ccccTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGaa                                                                                                                              <  7:236542/149‑1 (MQ=255)                          cTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACTGGAAGATGATTCAGGCGAATGAACAg                                                                                                                           >  5:59006/1‑149 (MQ=255)                          cTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACTGGAAGATGATTCAGGCGAATGAACAg                                                                                                                           >  5:53341/1‑149 (MQ=255)                            aaGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACTGGAAGATGATTCAGGCGAATGAACAGGc                                                                                                                         <  6:59006/149‑1 (MQ=255)                            aaGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGc                                                                                                                         >  4:168438/1‑149 (MQ=255)                             aGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACTGGAAGATGATTCAGGCGAATGAACAGGc                                                                                                                         <  6:53341/148‑1 (MQ=255)                                  cAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATgc                                                                                                                    <  1:8726/148‑1 (MQ=255)                                   aGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATgcgc                                                                                                                  >  6:187046/1‑149 (MQ=255)                                    gTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCCGGCGAATGAACAGGCCATgcgcg                                                                                                                 >  2:24575/1‑149 (MQ=255)                                         acCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATAc                                                                                                            >  3:199422/1‑149 (MQ=255)                                              aTCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGc                                                                                                       <  3:350627/149‑1 (MQ=255)                                                  gAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCt                                                                                                     <  2:89150/147‑1 (MQ=255)                                                  gAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCt                                                                                                     >  1:89150/1‑147 (MQ=255)                                                     tcaCGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGAAGGCTTCCTCGGCCGCGATGGCTGTAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATc                                                                                                <  2:247900/147‑1 (MQ=255)                                                     tGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCAt                                                                                                                      >  2:172582/1‑127 (MQ=255)                                                     tGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCAt                                                                                                                      <  1:172582/127‑1 (MQ=255)                                                     tGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATc                                                                                                <  2:85355/149‑1 (MQ=255)                                                       aCGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAg                                                                                                                           <  5:289594/120‑1 (MQ=255)                                                       aCGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAg                                                                                                                           >  6:289594/1‑120 (MQ=255)                                                       aCGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCgg                                                                                              <  5:111068/149‑1 (MQ=255)                                                       aCGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGACTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCa                                                                                                                                        <  8:83166/107‑1 (MQ=255)                                                         gAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGt                                                                                             >  1:250574/1‑148 (MQ=255)                                                           aCATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTaa                                                                                           >  3:310332/1‑148 (MQ=255)                                                               gAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑c                                                                                     >  6:183840/1‑149 (MQ=255)                                                                     tctcAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCt                                                                                >  3:97546/1‑148 (MQ=255)                                                                     tctcAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑ACGCCt                                                                                >  6:239043/1‑148 (MQ=255)                                                                         aaCGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAgg                                                                            <  3:168438/148‑1 (MQ=255)                                                                          aCGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGtt                                                                          <  4:310332/149‑1 (MQ=255)                                                                           cGGCCTTGCCGCATTACTGATTCTGATGGCTTCCTCGGCCTCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTg                                                                         <  8:374299/149‑1 (MQ=255)                                                                               aTTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATAGGTAAAAAT‑CCCCCTGAGGTAGTca                                                                      >  3:271336/1‑147 (MQ=255)                                                                                      ccTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGc                                                              >  6:144344/1‑149 (MQ=255)                                                                                      ccTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCCCCTGAGGTTGTCCACTACCg                                                               >  5:100094/1‑148 (MQ=255)                                                                                       cTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGc                                                              >  8:74853/1‑148 (MQ=255)                                                                                       cTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCt                                                             <  5:183840/149‑1 (MQ=255)                                                                                          aCTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACg                                                          >  2:171152/1‑149 (MQ=255)                                                                                           cTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACgg                                                         <  6:162341/149‑1 (MQ=255)                                                                                            tGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTc                                                                       <  2:258571/134‑1 (MQ=255)                                                                                            tGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTc                                                                       >  1:258571/1‑134 (MQ=255)                                                                                            tGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACgg                                                         >  6:240184/1‑148 (MQ=255)                                                                                            tGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACgg                                                         >  2:116994/1‑148 (MQ=255)                                                                                              aTTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCAc                                                                    <  4:281446/135‑1 (MQ=255)                                                                                              aTTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCAc                                                                    >  3:281446/1‑135 (MQ=255)                                                                                              aTTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGaa                                                       <  7:74853/148‑1 (MQ=255)                                                                                              aTTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGaa                                                       <  8:248837/148‑1 (MQ=255)                                                                                              aTTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAAt                                                      <  2:250574/149‑1 (MQ=255)                                                                                              aTTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAAt                                                      >  7:366106/1‑149 (MQ=255)                                                                                               ttCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATg                                                     >  1:291822/1‑149 (MQ=255)                                                                                                   gATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATc                                                 >  1:297719/1‑149 (MQ=255)                                                                                                    aTGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGTCCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATc                                                 >  3:379718/1‑148 (MQ=255)                                                                                                       gCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAg                                              <  5:187046/148‑1 (MQ=255)                                                                                                           ccTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGtt                                         >  7:79245/1‑149 (MQ=255)                                                                                                            cTCGGCCGCTATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTACATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTg                                        <  4:199422/149‑1 (MQ=255)                                                                                                            cTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGtt                                         <  8:79245/148‑1 (MQ=255)                                                                                                             tCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACAGCTACGGAATGGATCTAGATGTTg                                        >  5:237701/1‑148 (MQ=255)                                                                                                               ggCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCa                                      >  1:395831/1‑148 (MQ=255)                                                                                                                  cgcgATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAg                                  <  8:366106/149‑1 (MQ=255)                                                                                                                   gcgATGGCTGAAGGTGGATCTGACCGCCTGGACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGt                                 >  1:32636/1‑149 (MQ=255)                                                                                                                   gcgATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGt                                 <  5:239043/149‑1 (MQ=255)                                                                                                                       tGGCTGAAGGTGGATCTGTCCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGAtt                             <  4:379718/149‑1 (MQ=255)                                                                                                                          cTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGAttcgt                          >  3:45043/1‑146 (MQ=255)                                                                                                                          cTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCt                          >  3:50181/1‑149 (MQ=255)                                                                                                                           tGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCAAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCtg                         >  1:74163/1‑149 (MQ=255)                                                                                                                             aaGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAATACCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCtgt                        <  1:116994/149‑1 (MQ=255)                                                                                                                             aaGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCtgt                        >  2:77560/1‑148 (MQ=255)                                                                                                                               ggTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGAc                     >  2:188308/1‑149 (MQ=255)                                                                                                                                  ggATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCtgt                        >  7:269043/1‑143 (MQ=255)                                                                                                                                  ggATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCtgt                        <  8:269043/143‑1 (MQ=255)                                                                                                                                       tGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGAccc             >  4:82498/1‑149 (MQ=255)                                                                                                                                        gACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGAccc             <  6:100094/148‑1 (MQ=255)                                                                                                                                                  aCGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCtgt  <  1:77560/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TCACCGGGGAGACAGGTGCTGCCCCCTAAGATTCAGTCACACCAAATCGGAGTGACGAACATGAAAGCTCTCAACGGCATTGCCGCCTTACTGATTCTGATGGCTTCCTCGGCCGCGATGGCTGAAGGTGGATCTGACCGCCTGCACGGGAAGATGATTCAGGCGAATGAACAGGCCATGCGCGCATACGCAGCCTCCATCGGTAAAAAT‑CCGCCTGAGGTTGTCCACTACCGCTACGGAATGGATCTAGATGTTGCAAAAGTGATTGCTGTGACCTCGACCCGGGGAAGCTGT  >  NC_002947/6128746‑6129039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑