Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,149,824 T→A 6.2% intergenic (‑2008/‑1166) PP_5393 ← / → PP_5395 metal‑binding chaperone/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,149,8240TA 6.2% 244.5 / 5.3 80intergenic (‑2008/‑1166)PP_5393/PP_5395metal‑binding chaperone/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/45);  new base A (2/3);  total (32/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TCGGCCCCGATGCGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGATGCGACCCCA  >  NC_002947/6149676‑6149963
                                                                                                                                                    |                                                                                                                                           
tCGGCCCCGATGCGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGAt                                                                                                                                             >  6:158251/1‑149 (MQ=255)
  ggCCCCGATGCGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGc                                                                                                                                           <  3:182964/149‑1 (MQ=255)
   gCCCCGATGCGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCg                                                                                                                                          >  6:352494/1‑149 (MQ=255)
    ccccGATGCGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCATCGGAGTCTACTTCGAAGCgg                                                                                                                                         <  2:268852/149‑1 (MQ=255)
       cGATGCGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCa                                                                                                                                      >  3:224020/1‑149 (MQ=255)
        gATGCGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCATCGGAGTCTACTTCGAAGCgg                                                                                                                                         <  5:380366/145‑1 (MQ=255)
        gATGCGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCATCGGAGTCTACTTCGAAGCgg                                                                                                                                         >  6:380366/1‑145 (MQ=255)
            cGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTg                                                                                                                                 <  2:104022/149‑1 (MQ=255)
             gACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTg                                                                                                                                 >  2:293192/1‑148 (MQ=255)
             gACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGc                                                                                                                                <  2:27907/149‑1 (MQ=255)
                  gCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGa                                                                                                                            >  5:147084/1‑148 (MQ=255)
                   ccGTCTTGGGGGCAAGGCCCAGTAGCGACTTGATGGCGTCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGaaa                                                                                                                          <  4:241549/149‑1 (MQ=255)
                   ccGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGCGCAACGTTAATGAGATAATGACTGCCGCGGCTTCGAAGTAGACTCCGATGGGGCCCTCCTGGATgc                                                                                                                            >  3:300647/1‑146 (MQ=255)
                   ccGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGaaa                                                                                                                          >  5:359218/1‑149 (MQ=255)
                   ccGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGaaa                                                                                                                          <  5:323650/149‑1 (MQ=255)
                   ccGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGa                                                                                                                            >  8:237574/1‑147 (MQ=255)
                    cGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAg                                                                                                                         <  1:193193/149‑1 (MQ=255)
                             ggCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGAt                                                                                                                                             >  2:196424/1‑120 (MQ=255)
                             ggCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGAt                                                                                                                                             <  1:196424/120‑1 (MQ=255)
                             ggCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCggg                                                                                                                 >  2:142694/1‑148 (MQ=255)
                             ggCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGa                                                                                                                <  3:194198/149‑1 (MQ=255)
                                     ccAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGTGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGc                                                                                                        <  6:386790/149‑1 (MQ=255)
                                     ccAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGTGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGc                                                                                                        <  6:392058/149‑1 (MQ=255)
                                     ccAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGCGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGc                                                                                                        <  4:63870/149‑1 (MQ=255)
                                     ccAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGc                                                                                                        <  1:167943/149‑1 (MQ=255)
                                     ccAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGc                                                                                                        <  4:39318/149‑1 (MQ=255)
                                        ggAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCgg                                                                                                      >  5:283284/1‑148 (MQ=255)
                                          aGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGaa                                                                                                   <  5:158251/149‑1 (MQ=255)
                                          aGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGaa                                                                                                   <  7:196468/149‑1 (MQ=255)
                                              aCTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACt                                                                                               >  5:135617/1‑149 (MQ=255)
                                               cTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACtg                                                                                              >  5:341822/1‑149 (MQ=255)
                                                ttGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACtgt                                                                                             >  3:103066/1‑149 (MQ=255)
                                                ttGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACtgt                                                                                             >  6:144173/1‑149 (MQ=255)
                                                   aTGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTgg                                                                                           <  8:298250/148‑1 (MQ=255)
                                                    tGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGcc                                                                                         >  6:335802/1‑149 (MQ=255)
                                                      gcggcgGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCg                                                                                        <  6:261336/148‑1 (MQ=255)
                                                      gcggcgGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCg                                                                                        <  7:237574/148‑1 (MQ=255)
                                                        ggcggAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCaa                                                                                     <  6:147084/149‑1 (MQ=255)
                                                        ggcggAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCaa                                                                                     >  1:207141/1‑149 (MQ=255)
                                                        ggcggAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGCCACAGTGTTCCCGCag                                                                                     <  4:1730/149‑2 (MQ=255)
                                                            gAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCt                                                                                 <  5:352494/149‑1 (MQ=255)
                                                               gTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGta                                                                              <  4:128160/149‑1 (MQ=255)
                                                               gTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGta                                                                              >  7:86570/1‑149 (MQ=255)
                                                                tttGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGtat                                                                             <  1:142694/149‑1 (MQ=255)
                                                                  tgtgAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGtata                                                                            <  1:100265/148‑1 (MQ=255)
                                                                   gtgAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAgg                                                                          >  1:392139/1‑149 (MQ=255)
                                                                   gtgAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAg                                                                           <  4:103066/148‑1 (MQ=255)
                                                                   gtgAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAg                                                                           <  3:72372/148‑1 (MQ=255)
                                                                    tgAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAgg                                                                          <  4:224020/148‑1 (MQ=255)
                                                                    tgAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGt                                                                         <  1:293192/149‑1 (MQ=255)
                                                                      aGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTAc                                                                       <  6:341822/149‑1 (MQ=255)
                                                                           gcCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTAcgccgc                                                                  <  7:303640/149‑1 (MQ=255)
                                                                           gcCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTAcgccg                                                                   >  1:202421/1‑148 (MQ=255)
                                                                             cTTGAGCTCGAGCATTTGGCCGAGCAACGTAAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAg                                                                <  8:157632/149‑1 (MQ=255)
                                                                                  gCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTa                                                           >  5:365196/1‑149 (MQ=255)
                                                                                   cTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTAc                                                          <  6:76849/149‑1 (MQ=255)
                                                                                        gCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCaaa                                                     <  6:136506/149‑1 (MQ=255)
                                                                                        gCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCaaa                                                     <  2:202421/149‑1 (MQ=255)
                                                                                          aTTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGta                                                                              <  6:257976/122‑1 (MQ=255)
                                                                                          aTTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGta                                                                              >  5:257976/1‑122 (MQ=255)
                                                                                               gCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTAt                                                >  8:222846/1‑147 (MQ=255)
                                                                                                       aCGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCca                                       >  2:267095/1‑148 (MQ=255)
                                                                                                         gTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACat                                    <  2:207141/149‑1 (MQ=255)
                                                                                                              tGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCAGATGCCGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCTGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCg                               >  4:283244/1‑149 (MQ=255)
                                                                                                                agaTAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCgg                              <  6:135617/148‑1 (MQ=255)
                                                                                                                    aaTGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAACCCTAGCAGCGTCCACATATTCGGGGTGc                         >  2:206582/1‑149 (MQ=255)
                                                                                                                     aTGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGc                         >  7:309105/1‑148 (MQ=255)
                                                                                                                     aTGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGc                         >  7:132527/1‑148 (MQ=255)
                                                                                                                        aCTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGAtt                     <  2:175143/149‑1 (MQ=255)
                                                                                                                            tggCCGCATCGGAGTCTACTTCGAAGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGc                         >  4:76583/3‑141 (MQ=255)
                                                                                                                            tggCCGCATCGGAGTCTACTTCGAAGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGc                         <  3:76583/139‑1 (MQ=255)
                                                                                                                            cAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACg                  <  6:365196/148‑1 (MQ=255)
                                                                                                                              gCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAAt               <  8:309105/149‑1 (MQ=255)
                                                                                                                                  cTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGAt           <  1:267095/149‑1 (MQ=255)
                                                                                                                                  cTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGAt           <  8:132527/149‑1 (MQ=255)
                                                                                                                                   ttCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGATg          >  5:366597/1‑149 (MQ=255)
                                                                                                                                   ttCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGATg          >  5:360654/1‑149 (MQ=255)
                                                                                                                                     cGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTAAAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGATGc         <  1:206582/148‑1 (MQ=255)
                                                                                                                                      gAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGATGCGa       <  6:359218/149‑1 (MQ=255)
                                                                                                                                         gTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGATGCGAccc    >  7:193226/1‑149 (MQ=255)
                                                                                                                                            gACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAATCCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGATGCGACCCca  <  5:335802/148‑1 (MQ=255)
                                                                                                                                               tCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGc                         >  3:105713/1‑122 (MQ=255)
                                                                                                                                               tCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGc                         <  4:105713/122‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
TCGGCCCCGATGCGACGGGCCGTCTTGGGGGCAAGGCCCAGGAGCGACTTGATGGCGGCGGAGGTTTGTGAGCGCGCCTTGAGCTCGAGCATTTGGCCGAGCAACGTTAATGAGATAATGACTGCAGCCGCTTCGAAGTAGACTCCGATGCGGCCATCCTGCATGAAAGTCGTCGGGAAAACCTGCGGGAACACTGTGGCCGCAACGCTGTATAGGTACGCCGCAGCCGTACCCAAACCTATCAGCGTCCACATATTCGGGCTGCGATTACGAATCGATGCGACCCCA  >  NC_002947/6149676‑6149963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑