Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,174,709	A→C	6.2%	intergenic (‑71/+48)	glmU ← / ← atpC	bifunctional N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/ATP synthase subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,174,709	0	A	C	6.2%	203.3 / 3.9	65	intergenic (‑71/+48)	glmU/atpC	bifunctional N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/ATP synthase subunit epsilon
Reads supporting (aligned to +/- strand):  ref base A (32/29);  new base C (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

CGGGTGCAACACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCCGCGCCTTTCAGA  >  NC_002947/6174561‑6174856                                                                                                                                                     |                                                                                                                                                     cGGGTGCAACACTTAGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGCGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGa                                                                                                                                                      <  2:291760/149‑1 (MQ=255)  gggTGCAACACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAACGGGTAGCCGAg                                                                                                                                                     >  8:217453/1‑149 (MQ=255)     tGCAACACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGc                                                                                                                                                   >  2:311936/1‑148 (MQ=255)       cAACACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTAc                                                                                                                                                <  1:156844/149‑1 (MQ=255)       cAACACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTAc                                                                                                                                                >  6:323784/1‑149 (MQ=255)        aaCACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTAcc                                                                                                                                               <  2:338435/149‑1 (MQ=255)        aaCACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTAcc                                                                                                                                               >  1:338435/1‑149 (MQ=255)         acacTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGAGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTAccc                                                                                                                                              >  6:279057/1‑149 (MQ=255)            cTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCttt                                                                                                                                           <  7:217453/149‑1 (MQ=255)             tttGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCttt                                                                                                                                           >  3:209210/1‑148 (MQ=255)             tttGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCttt                                                                                                                                           >  2:338780/1‑148 (MQ=255)               tGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCTCGGCTACCCttttt                                                                                                                                         <  5:55284/148‑2 (MQ=255)               tGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCttt                                                                                                                                           <  4:209210/146‑1 (MQ=255)               tGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTa                                                                                                                                         <  5:323784/148‑1 (MQ=255)                gggCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATTGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACt                                                                                                                                       >  2:268069/1‑149 (MQ=255)                   cAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCGACCACTCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGACGAGGATACCCTTTTACTCaa                                                                                                                                    >  5:278736/1‑149 (MQ=255)                       gcCGAGCTCATGCGGGTGCCTTGGCCGGCGGCGTGAATAACGTTATCGAGTGTCATTACTGGCTACAAATCCTGGGCGGTAAGTGAAAGAGCGGGGGAGAACACAGATAATGTAAAAGGGTAGCCTCGGCTACCCTTTTTCtttttctg                                                                                                                                <  8:264292/149‑8 (MQ=25)                         cGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCaa                                                                                                                             <  8:19402/149‑1 (MQ=255)                          gAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCaa                                                                                                                             >  3:283188/1‑148 (MQ=255)                           aGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACa                                                                                                                           <  7:76801/149‑1 (MQ=255)                                  gCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGACTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTa                                                                                                                    >  4:70015/1‑149 (MQ=255)                                     ggTGCCTTGGCCGGCGGCGATTATAACGATATCGAGTGACATTACTTGCTACCAATCCTGGGCGGTCAGTGAAAGTCCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCa                                                                                                                 <  3:211386/149‑1 (MQ=255)                                       tGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGc                                                                                                               <  8:9025/149‑1 (MQ=255)                                          cTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAATGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCa                                                                                                                 >  3:40899/1‑144 (MQ=255)                                          cTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAATGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCa                                                                                                                 <  4:40899/144‑1 (MQ=255)                                          cTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCgg                                                                                                            >  1:393016/1‑149 (MQ=255)                                           ttGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAATAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCCGCCggg                                                                                                           >  2:356834/1‑149 (MQ=255)                                             ggCCGGCGGCGAGAATAACGATATCGAGTGAAATTACTTGCTACCAATCCTGGGCGGTCATTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCgggg                                                                                                          <  5:374791/148‑1 (MQ=255)                                              gCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGcc                                                                                                        <  6:118593/149‑1 (MQ=255)                                                   cggcgAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTa                                                                                                   <  5:140831/149‑1 (MQ=255)                                                      cgAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACtt                                                                                                <  2:393016/149‑1 (MQ=255)                                                      cgAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACtt                                                                                                <  1:172130/149‑1 (MQ=255)                                                      cgAGAATAACGATATCGAGTGAAATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGTCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACtt                                                                                                <  3:70015/149‑1 (MQ=255)                                                           aTAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCtt                                                                                           >  8:368115/1‑149 (MQ=255)                                                            tAACGATCTCGAGTGACATTACTGGCTACCAATCCTGGGCGGGCAGTGAAAGACCGGGGGAGAAAACAGAACAAGAAAAAGGGTCGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCttt                                                                                          <  6:278736/149‑1 (MQ=255)                                                                       aGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTATGCGCATTTGCt                                                                               >  7:271968/1‑149 (MQ=255)                                                                           aCATTACTGGCTACCAAAACTGGGCGGTCAGTCAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCCGCCGGGGCCGAGTACATGCCTTTCCGCATTTGCTGGAc                                                                           >  2:340819/1‑149 (MQ=255)                                                                            cATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGAct                                                                          <  7:368115/149‑2 (MQ=255)                                                                                  tGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACg                                                                     >  4:351685/1‑148 (MQ=255)                                                                                         cAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTggg                                                             >  7:11351/1‑149 (MQ=255)                                                                                         cAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTggg                                                             >  7:219542/1‑149 (MQ=255)                                                                                                gggCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCttt                                                                                          <  4:127077/113‑1 (MQ=255)                                                                                                    ggTCAGTGAAAGACCGGTGTAGAAAACATAAAAATAAAAAGGGTACCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATGAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCg                                                  <  5:194615/149‑1 (MQ=255)                                                                                                        aGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCTAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCCGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGc                                                >  4:377265/1‑147 (MQ=255)                                                                                                          tGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATAAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGa                                            >  3:383793/1‑149 (MQ=255)                                                                                                             aaGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGt                                         >  2:288540/1‑149 (MQ=255)                                                                                                               gACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTgc                                       <  1:288540/149‑1 (MQ=255)                                                                                                               gACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCCTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTgc                                       >  7:33000/1‑149 (MQ=255)                                                                                                                    ggggCGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTgcggcgg                                  <  2:102628/149‑1 (MQ=255)                                                                                                                    ggggAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTgcggcgg                                  >  6:38831/1‑149 (MQ=255)                                                                                                                    ggggAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTgcggcgg                                  >  2:37656/1‑149 (MQ=255)                                                                                                                          aaaaCAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCg                                                        <  4:186240/121‑1 (MQ=255)                                                                                                                          aaaaCAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCg                                                        >  3:186240/1‑121 (MQ=255)                                                                                                                                 aaaaagaaaaagGGTAGCCGAGGCTACCCTTTTACTCAATCAGCACCAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGt                      <  1:329098/149‑1 (MQ=255)                                                                                                                                    aagaaaaagGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGaa                  <  1:340819/149‑1 (MQ=255)                                                                                                                                      gaaaaagGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGcc                                               <  1:361300/118‑1 (MQ=255)                                                                                                                                      gaaaaagGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGcc                                               >  2:361300/1‑118 (MQ=255)                                                                                                                                       taaaagGGTAGCCTCGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTc               >  4:148289/2‑149 (MQ=255)                                                                                                                                        aaaagGGTAGCCTCGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTcc              >  8:13117/1‑149 (MQ=255)                                                                                                                                             ggTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCCGCGcc         <  4:283188/149‑1 (MQ=255)                                                                                                                                                aGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCCGCGCCtt       >  2:197266/1‑148 (MQ=255)                                                                                                                                                 gCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCCGCGCCttt      >  6:381470/1‑148 (MQ=255)                                                                                                                                                 gCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGAAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCCGCGCCttt      >  5:247239/1‑148 (MQ=255)                                                                                                                                                  ccGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCCGCGCCTTTCa    <  1:311936/149‑1 (MQ=255)                                                                                                                                                    gAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCCGCGCCTTTCAg   >  1:336711/1‑148 (MQ=255)                                                                                                                                                    gAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCCGCGCCTTTCAGa  <  5:279057/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CGGGTGCAACACTTTGGGCAGCGCCGAGCGCATGCGGGTGCCTTGGCCGGCGGCGAGAATAACGATATCGAGTGACATTACTGGCTACCAATCCTGGGCGGTCAGTGAAAGACCGGGGGAGAAAACAGAAAAAGAAAAAGGGTAGCCGAGGCTACCCTTTTACTCAATC‑GCAACAGAAGTTATCAGCCGGGGCCGATTACTTGCCTTTGCGCATTTGCTGGACGGTACGCAGCTGGGCTGCGGCCTCGGCCAGACGTGCGGCGGCGGCGCCGTAGTCGAAGTCCGCGCCTTTCAGA  >  NC_002947/6174561‑6174856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑