| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 499,203 | A→G | 100% | intergenic (+47/‑249) | uhpA → / → PP_0411 | two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 499,203 | 0 | A | G | 98.8% | 146.6 / ‑6.5 | 44 | intergenic (+47/‑249) | uhpA/PP_0411 | two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/23); minor base T (1/0); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTG > NC_002947/499058‑499326 | aaGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGc < 2:367663/149‑1 (MQ=255) gACCATCAGCCCCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCCCAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGCCATCCGGGTGCCCCAAACTTGACGAAGCCTATGT‑GG‑GAGCGGGCAt > 3:95635/1‑149 (MQ=255) cctCAGCACCCACCAGGCACGCCTGATGCAGAAGTTGAACGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGcc < 4:241725/147‑1 (MQ=255) cATCAGCACCCACAAGGCACGCATGATGCAGAAGTTGAAAGTGAACTAGCTGGCGGCGCTGGTGAAGTACGACATGGAGAACAAGCTGATCTGATTGAGACATACGGGTGCCCGAAACTTTCCGAATCATATGT‑TG‑GAGCGTGCATTcc > 5:95041/1‑149 (MQ=255) tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCcg > 3:88941/1‑149 (MQ=255) cAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCcgc > 4:293098/1‑149 (MQ=255) ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAACC‑‑TGT‑GG‑GAGCGGGCATGCCCGCGaagaggc > 1:11107/1‑144 (MQ=39) cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACggg > 5:327881/1‑149 (MQ=255) acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGggc < 3:183493/149‑1 (MQ=255) acaAGGCAAGCCTGATGCAGAAGTTGAAAGTGAACTAGCGGGCTGCGCTGGTGAAGTACGCCATGGAGCACAAGATGATCTGATTTCGACATACGGGTGACCGAAACTTGCCTAAGCCTATGT‑TG‑GAGCGGGCATGCCCTCGAAAGggc < 6:95041/149‑1 (MQ=255) aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAACC‑‑TGT‑GG‑GAGCGGGCATGCCcgc > 6:392931/1‑132 (MQ=255) aCGCCTGATGCAGAAGTTGAAAGTGAAATCGCTGGCGGAGATGGTGAAGTAAGCCATGGAGCACAAGCTGATATGATTGAGACATACGGGTGCCCGAAACTTGCCGAAGCCTAGTT‑GG‑GAGCTGTCATGCCCGCGAACGggcatggcct > 3:5578/1‑149 (MQ=255) aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAt < 3:293098/148‑1 (MQ=255) tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCGGCTGAtct > 7:47331/1‑149 (MQ=255) gCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtctc > 2:289946/1‑149 (MQ=255) cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtctc < 2:35688/148‑1 (MQ=255) aGTTGAAAGTTCACTCGCTGGGGGAGCTGGTTAATTACGCCATGGAGCACAAGCTTATCTTATTGCGACATACGTGTGCCCGAAACTTGCCGAAGCCTCTGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATg < 4:5578/148‑1 (MQ=255) aGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATgg < 8:168099/149‑1 (MQ=255) gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCCTGCCCGCGCACGGGCCTGGGCGGCGGGGCTCTATggg > 6:227936/1‑148 (MQ=255) ttGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACACGCCGATCTGATTGCGACATACGTGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCt < 4:95635/149‑1 (MQ=255) aaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAATCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGCGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGa < 1:289946/148‑1 (MQ=255) gTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCg > 6:25964/1‑149 (MQ=255) aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc < 6:327881/148‑1 (MQ=255) tCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCCTTCCCCCGCACCGGCCAGGGCTGCGGATGTATATTGCTTGGGCGGccgca > 5:8197/1‑146 (MQ=255) cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc < 5:25964/149‑1 (MQ=255) cTGGTAAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc < 3:51957/149‑1 (MQ=255) gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCg < 8:47331/149‑1 (MQ=255) tACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGCACGGGCCTGGCCGGCTGCTCTCTATGGCTGAGCCGGCCTCTTCGCGGGGATTCCCGCTACca > 4:347379/1‑149 (MQ=255) tACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGcgaaga < 6:106944/149‑6 (MQ=255) cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGCACGGGCCTGGCCAGCTGATCTCGATGGCTGAGCCGGCCGATTGGCGGGCATGACCGCTCCCCCAg > 7:98530/1‑147 (MQ=255) cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg > 4:329876/1‑129 (MQ=255) cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg < 3:329876/129‑1 (MQ=255) aTGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAt > 2:305759/1‑106 (MQ=255) aTGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAt < 1:305759/106‑1 (MQ=255) acaAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGGTTggtggt > 5:270437/1‑149 (MQ=255) tctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCGGATCTCTATGGCTGGGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTgg > 5:383116/1‑139 (MQ=255) tctgatTGCCACATACGGGTGCCCGAAACTTGCCGACGCCTATGT‑GG‑GCGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTgg < 6:383116/139‑1 (MQ=255) tgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGc > 1:273074/1‑147 (MQ=255) ttGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATgg < 1:301728/87‑1 (MQ=255) ttGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATgg < 1:30791/87‑1 (MQ=255) aTACGGGTGCCCGAAACTTGCCGACGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGt < 7:77208/128‑1 (MQ=255) aTACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc < 7:308726/90‑1 (MQ=255) aTACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc > 8:308726/1‑90 (MQ=255) aaCTTGCCCATCCCTTGGT‑GG‑GAGCGGCCAGGCCCGCTAAGGGGCCTGGCCTGCTGATCTCTATGGCGCCGCCGGCCTCTTCGCGGGCAGGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGACATTCCAATCCCGCCATCCTTg < 7:200914/149‑1 (MQ=255) | AAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GG‑GAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTG > NC_002947/499058‑499326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |