Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	508,775	A→T	6.9%	V79D (GTC→GAC)	estP ←	esterase EstP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	508,775	0	A	T	6.9%	209.0 / 5.2	73	V79D (GTC→GAC)	estP	esterase EstP
Reads supporting (aligned to +/- strand):  ref base A (35/32);  new base T (2/3);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

GCATTGGGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAACACGATCAGGG  >  NC_002947/508628‑508922                                                                                                                                                    |                                                                                                                                                     gCATTGGGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGAc                                                                                                                                                     <  6:284294/149‑1 (MQ=255)   aTTGGGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCTACGGGTTAAGGTCGCCTGGCGCGACGc                                                                                                                                                   <  7:146979/149‑1 (MQ=255)         gTTTCCTGGGGGGATGACGGTCTCGGACGTGGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATGGGGCTGGATACCTACGGATGTCGACGGGTTACGGTCGCCTGGCGCGCGGC‑CCAggc                                                                                                                                            >  4:198927/1‑149 (MQ=255)         gTTTCCTGGAGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAggc                                                                                                                                            <  5:336286/149‑1 (MQ=255)          tttCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGG‑GCGTCGCGCCAggcg                                                                                                                                           <  6:347880/149‑1 (MQ=255)            tCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGCCGCCTGG‑GCGTCGCGCCAggcga                                                                                                                                          <  4:278517/148‑2 (MQ=255)              cTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCa                                                                                                                                       >  7:201030/1‑149 (MQ=255)                   gggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAg                                                                                                                                  >  5:377340/1‑149 (MQ=255)                    ggaggaCGGTCTCGGACGTTGTCGTGATCGACTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAgg                                                                                                                                 <  3:198927/149‑1 (MQ=255)                    ggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAgg                                                                                                                                 <  7:182309/149‑1 (MQ=255)                    ggATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCGGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACGTCCGGATGTCGCCGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAgg                                                                                                                                 <  5:308253/149‑1 (MQ=255)                                 ggACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTAGTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTACGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGaca                                                                                                                    >  8:323891/1‑149 (MQ=255)                                 ggACGTTGTCGTGATCGAGTACAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGaca                                                                                                                    >  2:269902/1‑149 (MQ=255)                                      ttGTCGTGCTCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGGCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCggg                                                                                                               <  2:343546/149‑1 (MQ=255)                                         tCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGc                                                                                                             >  7:263786/1‑148 (MQ=255)                                           gTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCg                                                                                                            >  3:113582/1‑147 (MQ=255)                                              aTCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGtt                                                                                                       >  2:108876/1‑149 (MQ=255)                                                       aGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGc                                                                                              >  5:184874/1‑149 (MQ=255)                                                       aGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGc                                                                                              >  7:26751/1‑149 (MQ=255)                                                       aGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGG‑GCGTCGCGCCAggcgac                                                                                                                                         >  3:123507/1‑104 (MQ=255)                                                       aGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGG‑GCGTCGCGCCAggcgac                                                                                                                                         <  4:123507/106‑3 (MQ=255)                                                             tgctgGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACg                                                                                        >  7:316527/1‑149 (MQ=255)                                                               ctgGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCa                                                                                          <  6:377340/145‑1 (MQ=255)                                                               ctgGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACg                                                                                        <  1:365858/147‑1 (MQ=255)                                                               ctgGGGGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTTGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGt                                                                                      <  2:331167/149‑1 (MQ=255)                                                                   gtggtgTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGt                                                                                  <  7:340376/149‑1 (MQ=255)                                                                    tggtgTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTg                                                                                 <  8:316527/149‑1 (MQ=255)                                                                        gtACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAc                                                                             >  6:77767/1‑149 (MQ=255)                                                                         tACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAc                                                                             <  1:269902/148‑1 (MQ=255)                                                                          aCCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgc                                                                           >  2:211433/1‑149 (MQ=255)                                                                            ccgccgACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgcgc                                                                         <  8:37611/149‑1 (MQ=255)                                                                            ccgccgACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgcgc                                                                         >  5:250456/1‑149 (MQ=255)                                                                            ccgccgACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgcgc                                                                         >  5:249296/1‑149 (MQ=255)                                                                            ccgccgACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgcgc                                                                         >  5:144497/1‑149 (MQ=255)                                                                            ccgccgACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgcgc                                                                         >  2:122826/1‑149 (MQ=255)                                                                             cgccgACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAAcgcgcg                                                                        <  8:201030/149‑1 (MQ=255)                                                                              gccgACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACgcgcgc                                                                       >  4:88998/1‑149 (MQ=255)                                                                                   cTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTggg                                                                  <  8:12/149‑1 (MQ=255)                                                                                    tGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTgggg                                                                 >  6:306954/1‑149 (MQ=255)                                                                                       ccAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTAc                                                              >  6:128084/1‑149 (MQ=255)                                                                                          gTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGc                                                           <  4:333515/149‑1 (MQ=255)                                                                                              ttaCCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaa                                                        <  1:108876/148‑1 (MQ=255)                                                                                              ttaCCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaa                                                        <  3:88998/148‑1 (MQ=255)                                                                                                aCCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaaca                                                      <  6:144497/148‑1 (MQ=255)                                                                                                 ccATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGt                                                                                                  >  4:336975/1‑103 (MQ=255)                                                                                                 ccATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGt                                                                                                  <  3:336975/103‑1 (MQ=255)                                                                                                 ccATAGGGCTGGATACATACGGATGTCGACGGGTTAAGGTCGACTGTCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaacaa                                                     <  6:272033/148‑1 (MQ=255)                                                                                                  cATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCcaac                                                       >  5:234902/1‑145 (MQ=255)                                                                                                  cATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGa                                                   <  6:234902/149‑1 (MQ=255)                                                                                                    tCGGGCTGTATACCTACGGATGTCGACGGGTTAAGTTCGCCTGGAGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGTTACCGCCAACAAGATc                                                 <  8:263786/149‑1 (MQ=255)                                                                                                      gggCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCgg                                               <  5:77767/149‑1 (MQ=255)                                                                                                       ggCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCgg                                               >  3:250170/1‑148 (MQ=255)                                                                                                          tGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGaaa                                           >  1:135665/1‑149 (MQ=255)                                                                                                          tGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGaaa                                           >  5:357331/1‑149 (MQ=255)                                                                                                            gATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGaaa                                           <  4:353618/147‑1 (MQ=255)                                                                                                            gATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACt                                         >  4:204832/1‑149 (MQ=255)                                                                                                                 cTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATc                                                 <  5:207014/136‑1 (MQ=255)                                                                                                                 cTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATc                                                 >  6:207014/1‑136 (MQ=255)                                                                                                                 cTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCg                                    >  3:115196/1‑149 (MQ=255)                                                                                                                 cTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCg                                    >  2:39922/1‑149 (MQ=255)                                                                                                                 cTACGGATGTCGACGGGATAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCg                                    >  5:27305/1‑149 (MQ=255)                                                                                                                  tACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATAGGGAAACTGCCCgg                                   >  2:267485/1‑149 (MQ=255)                                                                                                                            gACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGa                          <  6:184874/148‑1 (MQ=255)                                                                                                                            gACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGa                          <  6:250456/148‑1 (MQ=255)                                                                                                                            gACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGa                          <  5:306954/148‑1 (MQ=255)                                                                                                                                 gTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGt                    <  1:211433/149‑1 (MQ=255)                                                                                                                                  ttAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTc                   >  1:151622/1‑149 (MQ=255)                                                                                                                                     aGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCCTCGGCGAGGCTGTCGc                 >  4:309625/1‑148 (MQ=255)                                                                                                                                      ggccGCCTGG‑GCGTCGCGCCAGGCGACCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGcc                >  2:323080/4‑148 (MQ=255)                                                                                                                                       gTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCa               >  6:75963/1‑148 (MQ=255)                                                                                                                                            cTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAacac          >  7:260512/1‑148 (MQ=255)                                                                                                                                            cTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAacac          >  1:255703/1‑148 (MQ=255)                                                                                                                                                   gACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAACACGATCAggg  <  8:260512/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCATTGGGGTTTCCTGGGGGGATGACGGTCTCGGACGTTGTCGTGATCGAGTCCAGGATCTGCTGGGTGGTGTACCCGCCGACTGCCCAGTTATTACCATCGGGCTGGATACCTACGGATGTCGACGGGTTAAGGTCGCCTGGCGCGACGC‑CCAGGCGGCCACCGAGGATCATCGGCGACACCGGGGCGAAGTTGCCGTCGGCGTCACGGTTGGTGAAACGCGCGCCTGGGGTACCGCCAACAAGATCGGGAAACTGCCCGGCATCGGCGAGGCTGTCGCCAAACACGATCAGGG  >  NC_002947/508628‑508922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑