Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 516,912 C→T 7.2% R27H (CGC→CAC)  PP_0429 ← short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947516,9120CT 7.2% 202.3 / 4.4 69R27H (CGC→CAC) PP_0429short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (29/35);  new base T (2/3);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

CTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTGCCTTTTG  >  NC_002947/516766‑517058
                                                                                                                                                   |                                                                                                                                                  
cttcCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTgc                                                                                                                                                    <  3:253919/147‑1 (MQ=255)
   ccAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGCCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCCGCGTATCTCGATGGCGTGCCACCAGGATCA‑GATTGCGCCcg                                                                                                                                               >  8:358032/1‑149 (MQ=255)
     aGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGATCAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCcgcg                                                                                                                                             <  8:250287/149‑1 (MQ=255)
     aGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCACCcgcg                                                                                                                                             >  7:229150/1‑149 (MQ=255)
        tctAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCg                                                                                                                                          >  1:23925/1‑149 (MQ=255)
          tAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTg                                                                                                                                        >  1:366088/1‑149 (MQ=255)
            gCACGAAGCCTGAAAGGCGCAGCGGCTGACTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGt                                                                                                                                         <  6:305400/146‑1 (MQ=255)
             cACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTCGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCCTTGGCGTGCCACCAGGAGCA‑GATTGCGCCCGCCCCGTGCCa                                                                                                                                     >  5:83741/1‑149 (MQ=255)
                     cTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAAGAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTcc                                                                                                                             <  2:237103/149‑1 (MQ=255)
                     cTGAAAGGCGCAGCGGCTGGCTGAGGGCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTcc                                                                                                                             <  3:89031/149‑1 (MQ=255)
                      tGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTGTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCg                                                                                                                            >  1:323428/1‑149 (MQ=255)
                      tGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCg                                                                                                                            >  5:205130/1‑149 (MQ=255)
                          aGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCaa                                                                                                                        <  7:291263/149‑1 (MQ=255)
                              gcAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAaggcc                                                                                                                    <  2:230765/149‑1 (MQ=255)
                              gcAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAaggc                                                                                                                     >  6:306548/1‑148 (MQ=255)
                                 gCGGCTGGCTGATGTCGCAGGCGCGGAACAGCACCTCGTCGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGTGCCCGCGCCGTGCCAGCGCTTCCGCCAatgccagg                                                                                                                 <  4:285033/149‑1 (MQ=255)
                                  cGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAaggccaggc                                                                                                                <  3:173188/149‑1 (MQ=255)
                                       ggctgAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGccaggcccagg                                                                                                           >  8:268638/1‑149 (MQ=255)
                                        gctgAGGTCGCATGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGccaggcccag                                                                                                            <  8:196591/147‑1 (MQ=255)
                                          tgAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCCGGCCCAGGCCg                                                                                                        >  2:303387/1‑149 (MQ=255)
                                             ggTCGCAGGCGCTGAAAAGCAACTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTg                                                                                                     <  8:171306/149‑1 (MQ=255)
                                             ggTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTg                                                                                                     <  8:319784/149‑1 (MQ=255)
                                                cGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGaa                                                                                                  <  6:178379/149‑1 (MQ=255)
                                                 gCAGGCGCGGAACAGCACCTCGACGCCGTATCGCTGGGGCAGGTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGTTCA‑GTTTGCGCCCGCGGCGTGCCAGCGCTTCCGCCAAGGGCAGGGCGAGGCCGCTGGaa                                                                                                  <  6:83741/148‑1 (MQ=255)
                                                 gCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAg                                                                                                 <  5:306548/149‑1 (MQ=255)
                                                       gcgGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAg                                                                                           >  5:84624/1‑149 (MQ=255)
                                                         ggAACAGCACCTCGACGCCGAAACTCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGt                                                                                          >  5:174309/1‑148 (MQ=255)
                                                                cACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATgg                                                                                  >  1:330559/1‑149 (MQ=255)
                                                                 aCCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGTGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCAt                                                                                    <  6:314446/146‑1 (MQ=255)
                                                                 aCCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGTGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCAt                                                                                    <  2:242219/146‑1 (MQ=255)
                                                                 aCCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGTGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCAt                                                                                    >  5:314446/1‑146 (MQ=255)
                                                                 aCCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGTGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCAt                                                                                    >  1:242219/1‑146 (MQ=255)
                                                                     cGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTaa                                                                             >  7:10922/1‑149 (MQ=255)
                                                                        cGCCGAAACTCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACgg                                                                          <  6:174309/149‑1 (MQ=255)
                                                                        cGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACgg                                                                          <  1:226521/149‑1 (MQ=255)
                                                                           cGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGt                                                                        >  2:34097/1‑148 (MQ=255)
                                                                            gAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCa                                                                      <  2:23925/149‑1 (MQ=255)
                                                                             aaaCGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCACCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCAt                                                                     <  8:229150/149‑1 (MQ=255)
                                                                                 gCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCaa                                                                 <  2:246827/149‑1 (MQ=255)
                                                                                 gCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCaa                                                                 <  6:114959/149‑1 (MQ=255)
                                                                                       tCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTcc                                                           >  5:321298/1‑149 (MQ=255)
                                                                                           cTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTggg                                                       <  3:35279/149‑1 (MQ=255)
                                                                                            tCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTgggg                                                      >  3:102750/1‑149 (MQ=255)
                                                                                               aTGGCAACAGGCTCCAGCGTTTCTCGTTGGCGGGCCACCAGTATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGGTGGAATCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCg                                                   <  3:27513/149‑1 (MQ=255)
                                                                                               aTGGCAACAGGCTCCAGCGTTTCTAGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCg                                                   <  5:301819/149‑1 (MQ=255)
                                                                                                 ggCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCgg                                                  >  2:168510/1‑148 (MQ=255)
                                                                                                    aaCAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGaa                                                >  7:16682/1‑147 (MQ=255)
                                                                                                           tCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCAGGATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCAt                                                                     >  1:53058/1‑120 (MQ=255)
                                                                                                             cAGCGTTTTTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTa                                     <  3:285466/149‑1 (MQ=255)
                                                                                                             cAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTGCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTTACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTa                                     <  7:358032/149‑1 (MQ=255)
                                                                                                             cAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTa                                     <  7:106964/149‑1 (MQ=255)
                                                                                                              aGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAg                                    >  6:21667/1‑149 (MQ=255)
                                                                                                              aGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAg                                    <  4:102750/149‑1 (MQ=255)
                                                                                                               gCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAgt                                   >  1:76947/1‑149 (MQ=255)
                                                                                                                  tttCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTa                                <  2:330559/149‑1 (MQ=255)
                                                                                                                      tcGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAAc                            >  5:313516/1‑149 (MQ=255)
                                                                                                                        gTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACtt                          <  6:313516/149‑1 (MQ=255)
                                                                                                                            gCGTGCCACCAGGATCA‑GCTTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGccc                      <  5:317088/149‑1 (MQ=255)
                                                                                                                            gCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGccc                      <  2:366088/149‑1 (MQ=255)
                                                                                                                            gCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACCGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTTccc                      >  5:327120/1‑149 (MQ=255)
                                                                                                                                 ccaccaGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGc                  >  5:12674/1‑148 (MQ=255)
                                                                                                                                   accaGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAg               <  2:188031/149‑1 (MQ=255)
                                                                                                                                        gATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGtt          >  2:286438/1‑149 (MQ=255)
                                                                                                                                         aTCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTg         >  1:114979/1‑149 (MQ=255)
                                                                                                                                         aTCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTg         >  3:20403/1‑149 (MQ=255)
                                                                                                                                           cA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTGcc       >  6:398596/1‑149 (MQ=255)
                                                                                                                                            a‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTGcc       <  8:16682/148‑1 (MQ=255)
                                                                                                                                               aTTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTGCCttt    <  6:321298/149‑1 (MQ=255)
                                                                                                                                                ttGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTGCCtttt   <  6:12674/149‑1 (MQ=255)
                                                                                                                                                ttGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTGCCtttt   <  5:398596/149‑1 (MQ=255)
                                                                                                                                                  gcgcCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTGCCTTTTg  <  5:21667/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACGCCGAAACGCTGGGTCAGCTCGATGGCAACAGGCTCCAGCGTTTCTCGTTGGCGTGCCACCAGGATCA‑GATTGCGCCCGCGCCGTGCCAGCGCTTCCGCCAAGGCCAGGCCCAGGCCGCTGGAAGCACCAGTGATCATGGCGTAACGGGTCATGCAAGGCTCCTGGGGGCGGAAGAGGGCGCCTAGTGTACAACTTGCCCGTGCAAGGTGTTGCCTTTTG  >  NC_002947/516766‑517058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑