| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 524,178 | C→G | 20.4% | intergenic (+45/‑771) | tyrS → / → PP_16SC | tyrosine‑‑tRNA ligase/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 524,178 | 0 | C | G | 20.4% | 83.9 / 3.7 | 42 | intergenic (+45/‑771) | tyrS/PP_16SC | tyrosine‑‑tRNA ligase/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (4/29); new base G (7/2); total (11/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.56e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCAC > NC_002947/524036‑524326 | cAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATCGAAGCGGGGAGGCCGG‑TAGGCCTccc < 1:210950/148‑1 (MQ=255) gTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGCCGC‑GAGGccagccccc > 1:359902/1‑142 (MQ=255) gTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGc < 4:251947/149‑1 (MQ=255) aGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTg < 1:11841/149‑1 (MQ=255) ttCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGCCGGATGGGCTGCCCCCA‑TTGGTTTTTGGGGt > 4:327230/1‑149 (MQ=255) tGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGGTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTtggt < 4:86338/149‑1 (MQ=255) gTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGGGGG‑TTGGCCGGCCCGC‑TGTGTTTTTGGGGTTtgggg > 6:88461/1‑147 (MQ=255) gTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGGCGG‑GGGGGCGGCCCGCGATGGTTTTTGGGGTTtggt > 1:394612/1‑149 (MQ=255) ttctttGGGGATACCCTTGTGTGCAAACCTGGAAATACGTCTTTGGGCGGGGTTCCCCTCATGGGTGAGTTATGGCCTGTTGGGTTTAGATATATACACGGGGGGCGGCCGT‑TAGGCCCCCCCGC‑TTTGTTTTTGGGGTTTGGTGGtt < 6:56258/144‑1 (MQ=255) gTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTTGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGtt < 3:327230/148‑1 (MQ=255) tCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTg < 5:312138/148‑1 (MQ=255) cTTGGTGATACCCATGTGTGCCAAGCGGGCAATAAGTCGTTTGGCGGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATTTAGAAGGGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCtg < 4:333984/149‑1 (MQ=39) ttGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGGGGG‑GAGGGCCCGCCCC‑CGTGTTTTTTggggttgggggttgggtg > 2:145794/1‑145 (MQ=255) gCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGCGGGCGG‑GGGGGCAGCCCGC‑GGTGTGTTTGGGGGTTGGTGGTTGGGGTTGTag > 4:288035/1‑149 (MQ=255) tACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTaggg < 1:169076/149‑1 (MQ=255) ccATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGCAGCGGG‑GAGGCCTCCCCCC‑GTTTTTTTTGTGGGATGTGGGTGTCTGTTGTagtggga > 4:243653/1‑149 (MQ=255) aTGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCACAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGCCGC‑TAGGGCGGACCCC‑GTTTTTTTTTGGGGTTGGTGGTTGGGGTTGTtggggggcg > 1:272473/1‑146 (MQ=255) tgtgCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTagggggagggg < 5:139397/148‑1 (MQ=255) tgCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGGGCCGG‑GAGGGGGGCCCCC‑CGTTTTTTTTTGGTTTGGTGGGTGGTGTTGTtgtggggggggggg > 5:5656/1‑147 (MQ=255) gggCAAGAAGTCGTTTGGTCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGGAGCCCG‑TAGTGCTGCCCGC‑GTTGTTTTTTGGGGTTTGTGGTTGGGGTTGTATTGGGGGGGTGGGGtttt > 1:266741/1‑146 (MQ=255) ggCAATAAGTCGTTTGGCCGGGTTACCCTCACGGCTGAGTGATTGCCTGGATGGGGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTgaga < 1:145794/149‑1 (MQ=255) ggCAAGAATTGGTTTGGTCGGGTTACCCTCAAGGCTGAGTGATTGCGTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTgaga < 2:266741/149‑1 (MQ=255) cAAGCAGTCGTTTGGCCGGTTGACCGTCAAGGAGGCGTGCTTGCCTGGATGGGGGAGCTATATAGAAGCGGGGCGGACGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAgg < 5:117096/149‑1 (MQ=37) cAAGAAGGCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGTTGGCCTGGATGGTGTAGATATATAGACGCGGGGAGGCCTG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAgg < 3:30018/149‑1 (MQ=38) aaagaatTCGTTAGCCCGGGGTACCCACCAGGATGTCTGATTGCCTGGATGGTGTAGGTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAgg < 3:243653/148‑1 (MQ=37) tCGTTTGGCCGGGTTACCCTCAAGGCTTAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTgg < 3:64547/149‑1 (MQ=255) ggggTTCCCCGCAAGGCTTCGTGATTGCTGGGTGGGTGCAGCTAAATACACCCGGGGCGTCGGC‑TAGGCCGCCGGGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGTGTGTTGGCGGTGAGAt < 8:239312/148‑1 (MQ=255) ctggTTAGCCTCATGGGTGAGTTTTTGCCTGTTTGGGGTAGCTATTTCGTAGCGGGGAGGCGTT‑TTGGGCTGCGCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGTGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGAt < 3:158365/147‑1 (MQ=19) cgtgTTACCCTCCAGGCTGTGTGATTGCCGGGTTGGTGTAGTTATTTAGAAGCGGGTAGGCGGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGAt < 2:359902/146‑1 (MQ=37) cGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGAt < 5:88461/149‑1 (MQ=255) ggTTACCCTCAACGCTGAGTGATTGCCTGGCTGGTGTAGCTATATATAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGAtgt < 7:257772/149‑1 (MQ=255) ggCTGAGTGATTGCCTGGATGGTGTAGCTATTTAGAAGCGGGGATGGCGG‑GAGGGCGACCCGC‑GTTGGTTTTTGGGTTTGTGGGGTGGTGTTGTAAGGGGAGCGGGGTGTTTTGACGTGCTGTTGGGTGTGCTATGTGTCGGCCCCAAc > 8:280555/1‑149 (MQ=255) tgtgtTTGCCTGTAGTGTTTAGCTATTTAGAAGCTGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTg < 8:320787/148‑1 (MQ=21) ttGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑GAGGCCTCCCCGG‑GTTGTTTTTGGGGTTTGGTGGTTGGTGGTGTAGGGGGAGGGGGCTGGTTTGAGAGGGTGTTGGCGGTGAGATTTGTGCGCCCCAACCCATGCAacg > 5:378695/1‑149 (MQ=255) gggtAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGt < 6:390080/107‑1 (MQ=255) tgtAGCTATATAGAAGCGGGGTGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACATCCCTTGCAGCGCCTGTGAGATc < 2:105203/148‑1 (MQ=255) tgtAGCTATATAGAAGCGGGGAGGCCGG‑GAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATc < 7:280555/148‑1 (MQ=255) gcaGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTAGGGGTTTGGTGGTTGCTGTTTTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGATATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGccgcccgc < 2:272473/147‑1 (MQ=255) ggggCGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTGGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCg < 5:210166/149‑1 (MQ=255) tggCCGG‑TAGGCCCCCCCGC‑TTTGTTTTTGGGGTGTGGTGGTTGCTGTTCTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCtt < 7:138798/148‑1 (MQ=255) ggCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGGGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTg < 6:378695/149‑1 (MQ=255) gcggg‑TCGGCCGCCCCGC‑TTTGTTTTTGGGGGTTGGGGGGTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGGGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTga < 5:231638/146‑1 (MQ=21) gcctggaGGGCTCGCCGC‑TTTGTTTTTGGCGTTTGGGGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCTCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTga < 5:236908/143‑1 (MQ=21) ccTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCAc > 2:28419/1‑149 (MQ=255) | CAGTGGTCGACAAGGACTTCATGTTTGTCCTTGGTGCTACCCATGTGTGCCAAGCGGGCAAGAAGTCGTTTGGCCGGGTTACCCTCAAGGCTGAGTGATTGCCTGGATGGTGTAGCTATATAGAAGCGGGGAGGCCGG‑TAGGCCTCCCCGC‑TTTGTTTTTGGGGTTTGGTGGTTGCTGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCAC > NC_002947/524036‑524326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |