Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	548,908	G→T	5.2%	L586L (CTG→CTT)	fusA →	elongation factor G 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	548,908	0	G	T	5.2%	311.5 / 2.6	98	L586L (CTG→CTT)	fusA	elongation factor G 1
Reads supporting (aligned to +/- strand):  ref base G (50/42);  new base T (3/3);  total (53/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTCGAAGTTGTAACCC  >  NC_002947/548762‑549056                                                                                                                                                     |                                                                                                                                                     gacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                                                                                                                                    <  6:398309/149‑1 (MQ=255) gacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                                                                                                                                    <  4:37666/149‑1 (MQ=255)   cAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAgg                                                                                                                                                  >  5:198307/1‑149 (MQ=255)        gCAACATCACAGAAGGTCTGGTGTTCTCCATCGAAGTCGTTGGTGGTTTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCTTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCcgc                                                                                                                                              <  7:11594/148‑1 (MQ=255)             aTCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                                                                                                                        >  6:340474/1‑149 (MQ=255)                acaGTAGGTCTGGTGTTCTACATCGAAGTCGTTGGTGGTTTGATTCCTAAGGAATACATCCCTGCGATCGAGAAGGGTATCGAA‑‑GAGCAGATGTAAATCGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGa                                                                                                                                     <  2:378880/149‑1 (MQ=255)                acaGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGa                                                                                                                                     <  1:21042/149‑1 (MQ=255)                acaGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGa                                                                                                                                     <  4:96071/149‑1 (MQ=255)                acaGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGa                                                                                                                                     >  8:224423/1‑149 (MQ=255)                  ataaGGTCCGGTGCTCTCCAACGAATTCGTTGGTTGTGTGTTTCCTAATGAATACATCCCTGCGATCCAGAATGGTATCGAATCCAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGa                                                                                                                                     <  1:314389/147‑1 (MQ=255)                             tgtTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAAGACATCCCTGCGATCCAGATGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTGATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGGTCGTACCac                                                                                                                        >  6:293167/1‑149 (MQ=255)                                 ctcCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTa                                                                                                                            >  3:144470/1‑141 (MQ=255)                                 ctcCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTa                                                                                                                            <  4:144470/141‑1 (MQ=255)                                 caccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCacgacg                                                                                                                    >  3:144/3‑149 (MQ=255)                                  tcCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGt                                                                                                                   >  2:32033/1‑149 (MQ=255)                                  tcCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGt                                                                                                                   >  7:102022/1‑149 (MQ=255)                                  tcCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGt                                                                                                                   >  3:136387/1‑149 (MQ=255)                                    cAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTc                                                                                                                  >  5:156811/1‑148 (MQ=255)                                    cAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTc                                                                                                                  >  7:28445/1‑148 (MQ=255)                                     aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGa                                                                                                                >  6:40942/1‑149 (MQ=255)                                     aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGa                                                                                                                <  6:47248/149‑1 (MQ=255)                                        gAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGa                                                                                                                <  2:230460/146‑1 (MQ=255)                                        gAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGa                                                                                                                >  1:230460/1‑146 (MQ=255)                                           gTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATACGACGGTTCGTACCACGACGTCGACTCCaa                                                                                                          <  1:32033/149‑1 (MQ=255)                                            tCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAAc                                                                                                         <  7:224423/149‑1 (MQ=255)                                            tCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAAc                                                                                                         <  6:162010/149‑1 (MQ=255)                                            tCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAAc                                                                                                         >  2:385126/1‑149 (MQ=255)                                                  ctggggTGATCCCTAAGGAATACATCCCTCCGATCCACAAGGGTATCGAA‑‑GAGCACATGAAAAACGGCGTTGTTGCCGGTTAGCCTCTCATCGGCCTGAAGGCCGCGGTATTCGCCGGTTCGTACCACGACGTCGACTCCAACGAAAt                                                                                                    <  4:199659/147‑1 (MQ=255)                                                    ggtgTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAAc                                                                                                         >  1:279614/1‑141 (MQ=255)                                                    ggtgTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAAc                                                                                                         <  2:279614/141‑1 (MQ=255)                                                        tgATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTCATGCCGGTTATCCTCTCATCGGCCTGAAGGCCGCGGTATTCGAGGGTTCCTACCACGACGTCGACTCCAACGAAATGGCGtt                                                                                              >  5:273774/1‑148 (MQ=255)                                                            tCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGCCTCCATCGATATGGCGTTc                                                                                             >  4:248260/1‑145 (MQ=255)                                                              cTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTc                                                                                             >  3:33118/1‑143 (MQ=255)                                                              cTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGAt                                                                                        <  4:108017/148‑1 (MQ=255)                                                               tAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTc                                                                                             >  4:43356/1‑142 (MQ=255)                                                                 aGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCgc                                                                                     <  6:156811/148‑1 (MQ=255)                                                                  ggAATAGATCCGTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTTAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCgctg                                                                                   <  7:183862/149‑1 (MQ=255)                                                                  cgAATACATCACTTCGATGGAGAATGGTATCGAA‑‑GAGCAGTTGAACAACGGCGTTGTTGCCGGTTATCGTGTGATCGGCCTGATGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCgctg                                                                                   <  6:273774/148‑1 (MQ=255)                                                                    aaTACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTc                                                                                                                  <  4:364612/116‑1 (MQ=255)                                                                    aaTACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCgctgct                                                                                 >  8:149091/1‑149 (MQ=255)                                                                     aTACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCtt                                                                                <  6:198307/149‑1 (MQ=255)                                                                       aCATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGCCTCCAACGAAATGGCGTTCAAGATCGCTGCTTc                                                                               >  4:262923/1‑148 (MQ=255)                                                                        cATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGa                                                                             <  1:250174/149‑1 (MQ=255)                                                                        cATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGa                                                                             >  3:76861/1‑149 (MQ=255)                                                                        cATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGa                                                                             >  3:71594/1‑149 (MQ=255)                                                                           cccTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATgg                                                                          >  8:229538/1‑149 (MQ=255)                                                                            ccTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGc                                                                         >  3:384690/1‑149 (MQ=255)                                                                            ccTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGc                                                                         >  1:12167/1‑149 (MQ=255)                                                                             cTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACAACGACGTCGACTCCAACGAAATGCCGATCAAGATCGCTGCTTCGATGGc                                                                         >  4:239555/1‑148 (MQ=255)                                                                              tGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGa                                                                       >  8:13607/1‑149 (MQ=255)                                                                                   tCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGAcc                                                                     >  6:58188/1‑146 (MQ=255)                                                                                   tCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCaa                                                                   <  5:58188/148‑1 (MQ=255)                                                                                    ccAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAg                                                                  >  2:31954/1‑148 (MQ=255)                                                                                     cAGAAGGGTATCGAA‑‑GAGCTGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTAGCACGACGTCGACTCCAACGAAATGGGGTTCAAGCTCGCTGCTTCGCTGGCGACCAAGc                                                                 >  6:276429/1‑148 (MQ=255)                                                                                             tataGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTg                                                            >  4:216105/1‑145 (MQ=255)                                                                                             tATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTg                                                            <  3:216105/145‑1 (MQ=255)                                                                                             tATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGcc                                                         >  6:66536/1‑148 (MQ=255)                                                                                              aTCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGcc                                                         >  7:364427/1‑147 (MQ=255)                                                                                               tCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGcc                                                         >  7:349854/1‑146 (MQ=255)                                                                                               tCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCa                                                       <  4:136387/148‑1 (MQ=255)                                                                                               tCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCa                                                       <  5:340474/148‑1 (MQ=255)                                                                                                cGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAg                                                      >  3:52665/1‑148 (MQ=255)                                                                                                   a‑‑gagCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAgg                                                  <  6:357474/149‑1 (MQ=255)                                                                                                   a‑‑gagCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAgg                                                  >  1:137763/1‑149 (MQ=255)                                                                                                         cAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCgg                                              <  3:43356/149‑1 (MQ=255)                                                                                                          aGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCTACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGt                                             >  8:143350/1‑149 (MQ=255)                                                                                                           gATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTg                                            <  8:28445/149‑1 (MQ=255)                                                                                                            aTGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCAAGAAGGGCGGTgg                                           >  7:242760/1‑149 (MQ=255)                                                                                                                aaaaCGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACGAAGCAACTGGCCCAGAAGGGCGGTGGCGTg                                       <  1:31954/149‑1 (MQ=255)                                                                                                                aaaaCGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTg                                       >  8:69296/1‑149 (MQ=255)                                                                                                                aaaaCGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTg                                       <  8:349854/149‑1 (MQ=255)                                                                                                                 aaaCGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTgg                                      >  6:120908/1‑149 (MQ=255)                                                                                                                 aaaCGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTgg                                      >  4:172503/1‑149 (MQ=255)                                                                                                                     ggCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTtct                                  <  5:66536/149‑1 (MQ=255)                                                                                                                      gCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAATGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTtctc                                 <  5:293167/149‑1 (MQ=255)                                                                                                                          tgttgCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGc                             <  8:102022/149‑1 (MQ=255)                                                                                                                           gttgCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGc                             <  8:364427/148‑1 (MQ=255)                                                                                                                              gCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGa                          <  5:40942/148‑1 (MQ=255)                                                                                                                              gCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGa                          <  4:52665/148‑1 (MQ=255)                                                                                                                                 ggTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCAt                      <  4:71594/149‑1 (MQ=255)                                                                                                                                 ggTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCAt                      <  4:76861/149‑1 (MQ=255)                                                                                                                                     aTCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGaa                   <  7:149091/148‑1 (MQ=255)                                                                                                                                     aTCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGa                    >  2:327211/1‑147 (MQ=255)                                                                                                                                      tCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGaaa                  <  5:120908/148‑1 (MQ=255)                                                                                                                                      tCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAg                 >  6:72995/1‑149 (MQ=255)                                                                                                                                        aataccgcGGCCTTCAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTTAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTc               >  4:17380/8‑149 (MQ=255)                                                                                                                                         tctGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTc               >  5:122883/1‑148 (MQ=255)                                                                                                                                         tctGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTc               >  4:174479/1‑148 (MQ=255)                                                                                                                                         ataccgcGGCCTTCAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTtct                                  <  5:45257/123‑1 (MQ=255)                                                                                                                                         ataccgcGGCCTTCAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTtct                                  >  6:45257/7‑129 (MQ=255)                                                                                                                                          taccgcGGCCTTCAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTCg              >  1:264963/6‑148 (MQ=255)                                                                                                                                             aTCGGCCTTAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCTACTCCAACTAAATGGCGTTCAAGATCGCTGCTTCGATGGCTACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCTATCATGAAAGTCGAAg           <  7:143350/148‑1 (MQ=255)                                                                                                                                             aTCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTCGaa            <  1:385126/147‑1 (MQ=255)                                                                                                                                              tCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGTTTCTCGAGCCGATCATGAAAGTCGAAGtt         <  3:172503/149‑1 (MQ=255)                                                                                                                                                   cTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTCGAAGTTGTAAc    >  3:157570/1‑149 (MQ=255)                                                                                                                                                    tGAAGGCCGCGGTATTCGACGGTTCGTACCCCGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTCGAAGTTGTAAcc   >  5:214742/1‑149 (MQ=255)                                                                                                                                                    tGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTCGAAGTTGTAAcc   >  6:364005/1‑149 (MQ=255)                                                                                                                                                    tGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTCGAAGTTGTAAcc   >  1:61670/1‑149 (MQ=255)                                                                                                                                                     gAAGGCCGCGGTATTAGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGAGCTGCCCTGGTTCTCGACCCGATCATGAAAGTCGAAGTTGAAAcac  >  8:285415/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAA‑‑GAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAACGAAATGGCGTTCAAGATCGCTGCTTCGATGGCGACCAAGCAACTGGCCCAGAAGGGCGGTGGCGTGGTTCTCGAGCCGATCATGAAAGTCGAAGTTGTAACCC  >  NC_002947/548762‑549056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑