Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 550,549 C→G 6.7% intergenic (+30/‑112) tufB → / → rpsJ elongation factor Tu‑B/30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947550,5490CG 6.7% 270.9 / 8.6 90intergenic (+30/‑112)tufB/rpsJelongation factor Tu‑B/30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base C (43/40);  new base G (3/3);  total (47/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

ATTCAGATGACTGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAA  >  NC_002947/550403‑550692
                                                                                                                                                    |                                                                                                                                                
aTTCAGATGACTGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAg                                                                                                                                                >  6:218498/1‑149 (MQ=255)
 ttCAGATGACTGTTACCCTGATCAAGGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAg                                                                                                                                                >  1:205360/1‑149 (MQ=255)
 ttCAGATGACTGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGc                                                                                                                                               >  8:285354/1‑149 (MQ=255)
    aGATGACTGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggg                                                                                                                                            >  8:297088/1‑149 (MQ=255)
      aTGACTGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggggg                                                                                                                                          <  7:247510/149‑1 (MQ=255)
         aCTGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGGGG‑tt                                                                                                                                       <  4:338365/148‑1 (MQ=255)
          cTGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑tttt                                                                                                                                     <  8:109332/149‑1 (MQ=255)
          cTGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑tttt                                                                                                                                     <  7:285354/149‑1 (MQ=255)
           tGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑ttttt                                                                                                                                    >  7:243421/1‑149 (MQ=255)
             ttACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑ttttt                                                                                                                                    <  8:257662/147‑1 (MQ=255)
                cccTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGGGG‑ttttttcaa                                                                                                                                <  6:317719/148‑4 (MQ=255)
                cccTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTa                                                                                                                                <  1:173276/148‑1 (MQ=255)
                cccTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTa                                                                                                                                <  5:143512/148‑1 (MQ=255)
                cccTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTAt                                                                                                                               >  7:200394/1‑149 (MQ=255)
                 ccTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTAtt                                                                                                                              >  6:6594/1‑149 (MQ=255)
                 ccTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTAtt                                                                                                                              >  4:195599/1‑149 (MQ=255)
                 ccTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCCCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTAtt                                                                                                                              >  5:85379/1‑149 (MQ=255)
                 ccTGATCAA‑GACCATCGCAATGGAAGACGGGCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTAtt                                                                                                                              <  3:125804/149‑1 (MQ=255)
                     cggatctaCCATCGCAATGGAAGACGGTCTGCGCTTCGCTAGCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTgg                                                                                                                            <  7:279321/141‑1 (MQ=255)
                     aTCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTgg                                                                                                                            <  7:297088/147‑1 (MQ=255)
                      tCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGtt                                                                                                                         <  5:218498/149‑1 (MQ=255)
                        aa‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGa                                                                                                                       >  6:121388/1‑149 (MQ=255)
                             ccATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑tttttt                                                                                                                                   >  3:127954/1‑133 (MQ=255)
                             ccATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑tttttt                                                                                                                                   <  4:127954/133‑1 (MQ=255)
                             ccATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATAATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTgg                                                                                                                            >  8:279321/1‑140 (MQ=255)
                              cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAGAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACt                                                                                                                   >  3:219302/1‑148 (MQ=255)
                               aTCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGt                                                                                                                          <  7:176708/140‑1 (MQ=255)
                               aTCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTaa                                                                                                                 >  3:149901/1‑149 (MQ=255)
                               aTCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACGCGAGCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTTTTGGGttagatcg                                                                                                                   >  8:176708/1‑141 (MQ=25)
                                tCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGac                                                                                                                      >  8:81265/1‑143 (MQ=255)
                                tCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGac                                                                                                                      <  7:81265/143‑1 (MQ=255)
                                   cAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTgg                                                                                                             <  2:205360/149‑1 (MQ=255)
                                    aaTGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTTATTggg                                                                                                            >  6:322437/1‑149 (MQ=255)
                                     aTGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTgggg                                                                                                           <  8:32892/149‑1 (MQ=255)
                                      tGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTTTTGGGTTGACACTAATTggggg                                                                                                          >  8:60253/1‑149 (MQ=255)
                                         aaGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGt                                                                                                       <  5:121388/149‑1 (MQ=255)
                                           gACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGACCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTc                                                                                                      >  2:237588/1‑148 (MQ=255)
                                                 ttgggcTTCGCTATCTGTGAAGGCGGTCGTACCGTCGGTGCCGGCGTTGTGGCTATAATGATCGA‑GTAATCACTGGACCGTTTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGa                                                                                                <  8:243421/144‑1 (MQ=38)
                                                 cTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggg                                                                                                                                            <  8:59767/105‑1 (MQ=255)
                                                 cTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggg                                                                                                                                            >  7:59767/1‑105 (MQ=255)
                                                     gcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCAc                                                                                           >  2:379591/1‑149 (MQ=255)
                                                     gcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCAc                                                                                           <  3:292324/149‑1 (MQ=255)
                                                      cTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGGGG‑ttttt                                                                                                                                    >  8:89279/1‑107 (MQ=255)
                                                       ttCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGc                                                                                         >  5:392471/1‑149 (MQ=255)
                                                         cGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAGAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGc                                                                                         <  4:219302/147‑1 (MQ=255)
                                                             aTCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCtt                                                                                   >  2:255607/1‑149 (MQ=255)
                                                              tCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCttt                                                                                  <  3:195599/149‑1 (MQ=255)
                                                              tCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTAAGCGGGGG‑TTTTTTTTATTGGGTTGACAATAATTGGGGGCGTCTATAGAATCTCGACTCCttt                                                                                  >  8:83874/1‑149 (MQ=255)
                                                               ccGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGAGGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCttt                                                                                  >  1:96637/1‑149 (MQ=255)
                                                                cGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGtt                                                                                                                         <  7:253955/108‑1 (MQ=255)
                                                                cGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGtt                                                                                                                         >  8:253955/1‑108 (MQ=255)
                                                                cGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCtttt                                                                                 <  4:19790/148‑1 (MQ=255)
                                                                         ggTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTa                                                                       <  8:200394/149‑1 (MQ=255)
                                                                          gTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAg                                                                      >  7:216071/1‑149 (MQ=255)
                                                                               aCCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTTTTGGGTTGACACTAATTTGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGt                                                                 >  2:126254/1‑149 (MQ=255)
                                                                               aCCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGt                                                                 >  1:234123/1‑149 (MQ=255)
                                                                                 cGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTcc                                                               >  8:210509/1‑149 (MQ=255)
                                                                                 cGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTcc                                                               <  4:149901/149‑1 (MQ=255)
                                                                                     ggCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTATAGCGGGCGTAGTGCGTCCGtt                                                            <  7:60253/148‑1 (MQ=255)
                                                                                      gcgcCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGTGGG‑TTTTTTTTATTGGGTTGACAATAATTGGGGGCGTCTATAGaa                                                                                               <  5:238316/112‑1 (MQ=255)
                                                                                      gcgcCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGaa                                                                                               >  6:238316/1‑112 (MQ=255)
                                                                                      gcgcCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTgg                                                          <  1:379591/149‑1 (MQ=255)
                                                                                       cgcCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTggg                                                         <  1:177717/149‑1 (MQ=255)
                                                                                          cGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGtt                                                            >  3:107489/1‑143 (MQ=255)
                                                                                          cGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGtt                                                            <  4:107489/143‑1 (MQ=255)
                                                                                            gCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAg                                                    >  7:55511/1‑149 (MQ=255)
                                                                                               ttGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTTTAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCt                                                 >  7:70984/1‑149 (MQ=255)
                                                                                               ttGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCt                                                 >  1:152668/1‑149 (MQ=255)
                                                                                                     ccAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTc                                           <  5:6594/149‑1 (MQ=255)
                                                                                                      cAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCt                                          >  4:62332/1‑149 (MQ=255)
                                                                                                      cAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCt                                          >  3:352555/1‑149 (MQ=255)
                                                                                                             atcGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGAGTCCGTTGGGAACAGCCTGGAGTCTGAAATc                                    >  8:242370/1‑148 (MQ=255)
                                                                                                                gA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATAACTCCTCCTTTTAGCGGGAGTAGTGCGTCCGTTGGTAACAGCATGGAGTATGAAATCaaa                                 >  2:150782/1‑149 (MQ=255)
                                                                                                                gA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTTTAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCaa                                 <  8:70984/148‑1 (MQ=255)
                                                                                                                gA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCaa                                 <  2:234123/148‑1 (MQ=255)
                                                                                                                gA‑GTAATCAATCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAAt                                <  7:83874/149‑1 (MQ=255)
                                                                                                                   gTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGc                              <  5:375144/149‑1 (MQ=255)
                                                                                                                        cACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATAACGCCTCCTTTTAGCGGGAGTAGTGCGTACGTTGGGAACAGCCTGGAGGCTGAAATCCAATGCaaaa                          >  8:167444/1‑148 (MQ=255)
                                                                                                                          cTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATc                        >  2:188708/1‑148 (MQ=255)
                                                                                                                             gaacGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGc                     <  6:85379/145‑1 (MQ=255)
                                                                                                                             gACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGc                     <  1:237588/148‑1 (MQ=255)
                                                                                                                                   ttGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCg              >  7:169724/1‑149 (MQ=255)
                                                                                                                                      aaaaaaCCCCCGCTGAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTa            >  4:166585/1‑148 (MQ=255)
                                                                                                                                       aaaaaCCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTAt           >  6:384737/1‑148 (MQ=255)
                                                                                                                                        aaaaCCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCa         <  1:188708/149‑1 (MQ=255)
                                                                                                                                           aCCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGc                     >  4:110156/1‑134 (MQ=255)
                                                                                                                                           aCCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGc                     <  3:110156/134‑1 (MQ=255)
                                                                                                                                               ccGCTGAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCaa                            <  1:389280/123‑1 (MQ=255)
                                                                                                                                               ccGCTGAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCaa                            >  2:389280/1‑123 (MQ=255)
                                                                                                                                               ccGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGaa  <  7:210509/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ATTCAGATGACTGTTACCCTGATCAA‑GACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGTGGCCAAAATCATCGA‑GTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAA  >  NC_002947/550403‑550692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑