Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 556,986 A→C 6.3% K12T (AAG→ACG)  rpsN → 30S ribosomal protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947556,9860AC 6.3% 298.2 / 9.5 95K12T (AAG→ACG) rpsN30S ribosomal protein S14
Reads supporting (aligned to +/- strand):  ref base A (40/49);  new base C (3/3);  total (43/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

ATGCTCTGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGC  >  NC_002947/556838‑557134
                                                                                                                                                    |                                                                                                                                                     
aTGCTCTGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGaa                                                                                                                                                       <  4:270150/149‑1 (MQ=255)
  gCTCTGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGc                                                                                                                                                     >  2:337651/1‑149 (MQ=255)
    tctGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGATAATGGCCAAGAAGAGCATGAAAAACCGCGACCTGAAGctt                                                                                                                                                   >  8:363849/1‑147 (MQ=255)
    tctGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑t                                                                                                                                                  >  5:321309/1‑149 (MQ=255)
      tGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACTAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑t                                                                                                                                                  <  3:253501/147‑1 (MQ=255)
      tGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑t                                                                                                                                                  <  4:134438/147‑1 (MQ=255)
        cgcgGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAg                                                                                                                                               >  4:287005/1‑148 (MQ=255)
             tgatctcATCACCCTGCCCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGCTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAATAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACg                                                                                                                                         <  1:140642/143‑1 (MQ=255)
               tGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCT‑TCAGCTCGCgg                                                                                                                                        <  4:350810/148‑1 (MQ=255)
               tGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCT‑TCAGCTCGCgg                                                                                                                                        <  4:298303/148‑1 (MQ=255)
               tGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACgg                                                                                                                                        <  6:125914/148‑1 (MQ=255)
                 gACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAg                                                                                                                                     >  4:95715/1‑149 (MQ=255)
                  aCATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAg                                                                                                                                     >  5:76384/1‑148 (MQ=255)
                    aTCACCCTGACCACCACTCCTCGTTCGGAACACGCAGGCCGCGCTCTGCTGCGTGCTTTCAAGCTCCCGTTCCGCAACAGATTCGAGTACGAAAATGGCCAAGAAGACCAACAAAAACCGCGACCAGAACCG‑TCAGCTCACgcacatcc                                                                                                                                  >  2:140642/1‑142 (MQ=255)
                     tCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCaaa                                                                                                                                 <  7:195633/149‑1 (MQ=255)
                      cACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAg                                                                                                                                >  6:250656/1‑149 (MQ=255)
                       aCCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGt                                                                                                                               <  7:363849/149‑1 (MQ=255)
                        cccTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGtt                                                                                                                              <  7:86272/149‑1 (MQ=255)
                        cccTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGtt                                                                                                                              <  1:210050/149‑1 (MQ=255)
                         ccTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTc                                                                                                                             >  6:26016/1‑149 (MQ=255)
                          cTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCg                                                                                                                            <  6:321309/149‑1 (MQ=255)
                             accaccACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCt                                                                                                                          <  5:250656/148‑1 (MQ=255)
                             accaccACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCCTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGc                                                                                                                           <  4:218772/147‑1 (MQ=255)
                                   acTGCTCGTTCGGACTACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCAGGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGc                                                                                                                   <  6:291886/149‑1 (MQ=255)
                                     tGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGtt                                                                                                                              <  7:219719/136‑1 (MQ=255)
                                     tGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGtt                                                                                                                              >  8:219719/1‑136 (MQ=255)
                                     tGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGt                                                                                                                 >  2:284832/1‑149 (MQ=255)
                                       cTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTg                                                                                                                <  5:88491/148‑1 (MQ=255)
                                        tCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgct                                                                                                              >  2:375006/1‑149 (MQ=255)
                                          gTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgctga                                                                                                            <  8:113104/149‑1 (MQ=255)
                                           ttCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgctgag                                                                                                           <  4:187913/149‑1 (MQ=255)
                                              ggACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgctgagct                                                                                                         >  2:22681/1‑148 (MQ=255)
                                               gacgacGAAAGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTgctgagctg                                                                                                        >  4:19202/1‑148 (MQ=255)
                                                acgacgAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCT‑TCAGCTCGCGGttttt                                                                                                                                   <  6:102758/120‑5 (MQ=38)
                                                acgacgAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGTGAGCTGACGCT‑TCAGCTCGCGGttttt                                                                                                                                   >  5:102758/1‑116 (MQ=38)
                                                 cgacgaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGaaa                                                                                                     <  7:260331/149‑1 (MQ=255)
                                                  gacgaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAg                                                                                                    <  3:287005/149‑1 (MQ=255)
                                                  gacgaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAg                                                                                                    >  3:274295/1‑149 (MQ=255)
                                                  gacgaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAg                                                                                                    >  6:222694/1‑149 (MQ=255)
                                                  gaagaATGCCGCGCTCTGCTGGGTGCTTTCAATTTCCCGTTCCGCAACTGTTTGGAGTATGAAAATGGCCAAGAACATCATGAAAAACCGCGAGCTGAAGCGCTCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGaaa                                                                                                     <  1:284832/146‑1 (MQ=255)
                                                     gaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAGACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGa                                                                                                 >  8:175493/1‑149 (MQ=255)
                                                     gaAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGa                                                                                                 <  3:76313/149‑1 (MQ=255)
                                                       aGGGCGCGCTCTGCTGCTTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCT‑AGAAGCGTGCTGAGCTGAAAGCGACCa                                                                                              <  3:209579/149‑1 (MQ=255)
                                                           cgcgCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCg                                                                                           >  4:352093/1‑149 (MQ=255)
                                                           cgcgCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCg                                                                                           >  8:295447/1‑149 (MQ=255)
                                                             cgcTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGt                                                                                          >  2:312325/1‑148 (MQ=255)
                                                             cgcTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTc                                                                                         <  3:19202/149‑1 (MQ=255)
                                                                tctGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAAc                                                                                      >  1:334889/1‑149 (MQ=255)
                                                                  tgctgcGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAAc                                                                                      <  3:352093/147‑1 (MQ=255)
                                                                       cGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAAc                                                                                <  1:312325/148‑1 (MQ=255)
                                                                       cGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAAc                                                                                <  7:295447/148‑1 (MQ=255)
                                                                        gTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTACGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGc                                                                              <  1:273298/149‑1 (MQ=255)
                                                                            tttCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCctct                                                                          >  8:83465/1‑149 (MQ=255)
                                                                            tttCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGGAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCctct                                                                          >  2:109553/1‑149 (MQ=255)
                                                                             ttCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCtctc                                                                         >  1:59723/1‑149 (MQ=255)
                                                                              tCAAGTTCCCGTTCCGCAACTGATTGGAGTAGG‑AAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCtctc                                                                         <  5:390756/147‑1 (MQ=255)
                                                                                aaGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCag                                                                      <  6:342953/149‑1 (MQ=255)
                                                                                aaGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCa                                                                       <  5:222694/148‑1 (MQ=255)
                                                                                  gTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCagaa                                                                    <  5:26016/149‑1 (MQ=255)
                                                                                  gTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCag                                                                      >  2:326495/1‑147 (MQ=255)
                                                                                      ccGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCGGAGCTGAAAGCGACCATCGTCACCCTGACCGCCTCTCCAGAAGAGc                                                                >  6:279500/1‑149 (MQ=255)
                                                                                      ccGGTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGc                                                                <  4:291856/149‑1 (MQ=255)
                                                                                       cGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCg                                                               >  6:300691/1‑149 (MQ=255)
                                                                                           ccGCAACTTATTGGAGTATGAAAATGGCAAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                            <  1:335076/148‑1 (MQ=255)
                                                                                           ccGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                            <  5:204661/148‑1 (MQ=255)
                                                                                           ccGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                            <  6:76384/148‑1 (MQ=255)
                                                                                           ccGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGttt                                                            <  2:334889/148‑1 (MQ=255)
                                                                                            cGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCTTGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCg                                                          <  1:143668/149‑1 (MQ=255)
                                                                                            cGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTc                                                           >  5:1512/1‑148 (MQ=255)
                                                                                            cGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTc                                                           <  6:1512/148‑1 (MQ=255)
                                                                                             gCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGc                                                         >  6:41244/1‑149 (MQ=255)
                                                                                             gCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGc                                                         <  1:337651/149‑1 (MQ=255)
                                                                                              cAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCt                                                        <  5:41244/149‑1 (MQ=255)
                                                                                                  tGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCg                                                    <  3:95715/149‑1 (MQ=255)
                                                                                                        gAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCg                                              >  6:1154/1‑149 (MQ=255)
                                                                                                           tAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGctc                                           >  7:225403/1‑149 (MQ=255)
                                                                                                             ggAAAATGGCCAAGAAGAGCATGAAAGACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGctct                                          <  7:175493/148‑1 (MQ=255)
                                                                                                             ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTg                                         <  1:326495/149‑1 (MQ=255)
                                                                                                             ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTg                                         >  1:352952/1‑149 (MQ=255)
                                                                                                             ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTg                                         <  1:22681/149‑1 (MQ=255)
                                                                                                             ggAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTg                                         >  2:352153/1‑149 (MQ=255)
                                                                                                              gAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGATCTg                                         >  6:133915/1‑148 (MQ=255)
                                                                                                               aaaaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCTTCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTg                                         >  3:22344/1‑147 (MQ=255)
                                                                                                               aaaaTGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCACCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGc                                        <  4:253466/148‑1 (MQ=255)
                                                                                                                     gCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAag                                   >  1:208451/1‑147 (MQ=255)
                                                                                                                       cAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagcagc                               >  4:370467/1‑149 (MQ=255)
                                                                                                                          gaagaGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCAc                            <  2:208451/149‑1 (MQ=255)
                                                                                                                              agCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGa                        <  1:352153/149‑1 (MQ=255)
                                                                                                                                     aaaaCCTCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGATCTGAAAGCGCCCATc                                                                                            >  2:194339/1‑74 (MQ=255)
                                                                                                                                     aaaaCCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATc                                                                                            <  1:194339/74‑1 (MQ=255)
                                                                                                                                     aaaaCCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAgc                 <  1:375006/149‑1 (MQ=255)
                                                                                                                                       taCCGTGAGCTGACGCT‑TCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAgcgc               >  2:152261/2‑149 (MQ=255)
                                                                                                                                       taCCGTGAGCTGACGCT‑TCAGCTCGCGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAgcgc               >  5:311390/2‑149 (MQ=255)
                                                                                                                                                 tGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagcag                                <  6:317797/122‑1 (MQ=255)
                                                                                                                                                 tGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAagcag                                >  5:317797/1‑122 (MQ=255)
                                                                                                                                                  gAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgc    >  4:205560/1‑149 (MQ=255)
                                                                                                                                                   aaGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgc    <  5:133915/148‑1 (MQ=255)
                                                                                                                                                    aGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGGTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTgcgc  <  5:300691/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGCTCTGCGCGGTTTGGACATCACCCTGACCACCACTGCTCGTTCGGACGACGAAGGCCGCGCTCTGCTGCGTGCTTTCAAGTTCCCGTTCCGCAACTGATTGGAGTAGGAAAATGGCCAAGAAGAGCATGAAAAACCGCGAGCTGAAGCG‑TCAGCTCACGGTAGCAAAGTTCGCTAAGAAGCGTGCTGAGCTGAAAGCGACCATCGTCAACCTGAACGCCTCTCCAGAAGAGCGTTTCGCTGCCGTTGTCGCTCTGCAGAAGCAGCCACGCGACGCTAGCGCCTCGCGCCTGCGC  >  NC_002947/556838‑557134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑