Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 616,451 T→A 8.1% noncoding (71/142 nt) PP_mr11 ← FMN regulatory region

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947616,4510TA 8.1% 176.1 / 4.9 62noncoding (71/142 nt)PP_mr11FMN regulatory region
Reads supporting (aligned to +/- strand):  ref base T (27/30);  new base A (2/3);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

AAACGCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGCAGCAGTAAC  >  NC_002947/616303‑616597
                                                                                                                                                    |                                                                                                                                                  
aaaCGCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACAt                                                                                                                                                    <  4:286614/149‑1 (MQ=255)
      tcctcGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCgg                                                                                                                                              >  1:24111/1‑149 (MQ=255)
       cctcGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCggg                                                                                                                                             <  3:285449/149‑1 (MQ=255)
       ccgcgggAATGAGCGATCAACGTCACGGGGCGAACAAACTGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCATCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCggg                                                                                                                                             <  8:340633/143‑1 (MQ=255)
        ctcGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCggg                                                                                                                                             <  6:288563/148‑1 (MQ=255)
        ctcGTGAATGATCGATGAACGTCTCGGGCCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCggg                                                                                                                                             <  5:347123/148‑1 (MQ=255)
         tcGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGgc                                                                                                                                           >  3:94805/1‑149 (MQ=255)
         tcGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGgc                                                                                                                                           >  8:235461/1‑149 (MQ=255)
           gTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACCATGTCgggggtc                                                                                                                                         <  4:92982/149‑4 (MQ=255)
           gTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACCATGTCggggg                                                                                                                                           <  8:237391/147‑2 (MQ=255)
           gTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGgcgc                                                                                                                                         >  7:208958/1‑149 (MQ=255)
                tGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTcgcg                                                                                                                                    >  5:75702/1‑149 (MQ=255)
                     gATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTc                                                                                                                               <  7:78417/149‑1 (MQ=255)
                            gTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTc                                                                                                                        >  8:64609/1‑149 (MQ=255)
                               tcGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACCATGTCgggggt                                                                                                                                          <  1:23140/128‑3 (MQ=255)
                               tcGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACCATGTCgggggt                                                                                                                                          >  2:23140/1‑126 (MQ=255)
                                 ggggCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGa                                                                                                                      <  1:234376/146‑1 (MQ=255)
                                 ggggCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGa                                                                                                                      <  6:361442/146‑1 (MQ=255)
                                 ggggCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGAttt                                                                                                                   <  2:24111/149‑1 (MQ=255)
                                 ggggCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGAtt                                                                                                                    <  1:315845/148‑1 (MQ=255)
                                  gggCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACACCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTa                                                                                                                  >  8:101603/1‑149 (MQ=255)
                                            aaTGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTgg                                                                                                        <  7:64609/149‑1 (MQ=255)
                                                   gcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTa                                                                                                 >  6:11408/1‑149 (MQ=255)
                                                   gcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTa                                                                                                 <  7:235461/149‑1 (MQ=255)
                                                      cAAGGGCGCCCGGATGGCCCCTGCATAACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTacac                                                                                              <  2:67645/149‑1 (MQ=255)
                                                                 ggATGGCCACTTCATGACGCCTTCTTTCATCCAGACTATGACGATTGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGaa                                                                                   <  1:329765/149‑1 (MQ=255)
                                                                        cccTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCTGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAgaccgac                                                                            >  5:299422/1‑149 (MQ=255)
                                                                        cccTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTc                                                                                                                        >  6:284248/1‑105 (MQ=255)
                                                                        cccTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTc                                                                                                                        <  5:284248/105‑1 (MQ=255)
                                                                        cccTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAgaccgac                                                                            >  7:293151/1‑149 (MQ=255)
                                                                            gCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAg                                                                        >  4:157518/1‑149 (MQ=255)
                                                                                 aCGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCCTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATa                                                                    >  3:137605/1‑148 (MQ=255)
                                                                                      ttcttTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATGGAGca                                                               >  1:137304/1‑148 (MQ=255)
                                                                                      ttcttTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGca                                                               >  3:101036/1‑148 (MQ=255)
                                                                                             aTCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGcc                                                        >  8:132372/1‑148 (MQ=255)
                                                                                                cGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGCCTTACCCCCCGCCCTGAAGACCGCCCCAGCATACAGCACAGTGCCAccc                                                    >  2:37966/1‑149 (MQ=255)
                                                                                                cGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCAccc                                                    <  6:299422/149‑1 (MQ=255)
                                                                                                 ggACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCAccc                                                    >  8:335021/1‑148 (MQ=255)
                                                                                                  gACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATg                                                                                                                                                   <  5:198454/52‑1 (MQ=255)
                                                                                                  gACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATg                                                                                                                                                   >  6:198454/1‑52 (MQ=255)
                                                                                                     tATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGTCATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTg                                               <  3:269276/149‑1 (MQ=255)
                                                                                                     tATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGTTTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTg                                               <  7:132372/149‑1 (MQ=255)
                                                                                                                gCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGcc                                                                                        >  6:228328/1‑97 (MQ=255)
                                                                                                                gCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGcc                                                                                        <  5:228328/97‑1 (MQ=255)
                                                                                                                 ctctGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGt                                   <  4:46927/149‑1 (MQ=255)
                                                                                                                      taGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCTCGGGCTCATCTTTCGATATACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTctac                              <  5:187076/148‑1 (MQ=255)
                                                                                                                        gTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTctacta                            <  4:94805/149‑1 (MQ=255)
                                                                                                                           gCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCCTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTAcc                          <  4:137605/148‑1 (MQ=255)
                                                                                                                              ccAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCcgcg                                     >  3:383034/1‑134 (MQ=255)
                                                                                                                              ccAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCcgcg                                     <  4:383034/134‑1 (MQ=255)
                                                                                                                               cAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCgg                     >  4:218918/1‑149 (MQ=255)
                                                                                                                                      gctgACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACt              >  6:284157/1‑149 (MQ=255)
                                                                                                                                       ctgACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCgaga                <  7:335021/146‑1 (MQ=255)
                                                                                                                                       ctgACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCgag                 <  7:101603/145‑1 (MQ=255)
                                                                                                                                          aCCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgca          <  8:208958/149‑1 (MQ=255)
                                                                                                                                          aCCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgc           <  4:631/148‑1 (MQ=255)
                                                                                                                                          aCCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgc           <  3:218918/148‑1 (MQ=255)
                                                                                                                                          aCCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGACCAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGTGACTTg            >  4:66491/1‑147 (MQ=255)
                                                                                                                                           gccccGACCATGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGGCCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgcag         >  2:50913/2‑149 (MQ=255)
                                                                                                                                            ccccGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgcag         >  2:351752/1‑148 (MQ=255)
                                                                                                                                            ccccGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgcag         >  1:71294/1‑148 (MQ=255)
                                                                                                                                             cccGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGt                                                >  8:90580/1‑108 (MQ=255)
                                                                                                                                                  cATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGCAGCAGTAAc  <  8:53534/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAACGCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGCAGCAGTAAC  >  NC_002947/616303‑616597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑