Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 675,591 A→T 7.4% A36A (GCT→GCA)  PP_0577 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947675,5910AT 7.4% 198.3 / 5.5 68A36A (GCT→GCA) PP_0577hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/30);  new base T (3/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

GTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTCC  >  NC_002947/675447‑675736
                                                                                                                                                |                                                                                                                                                 
gTTGCGCCAGCTCCTGCTTCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCAGTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAgccagcca                                                                                                                                               <  1:308358/149‑1 (MQ=255)
gTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccagc                                                                                                                                                 >  7:302886/1‑147 (MQ=255)
gTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGcagccagc                                                                                                                                                 >  4:90602/1‑147 (MQ=255)
gTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAgccagcca                                                                                                                                               <  7:95363/149‑1 (MQ=255)
   gcgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAgg                                                                                                                                             <  8:302886/148‑1 (MQ=255)
    cgcCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAggg                                                                                                                                            <  1:296730/148‑1 (MQ=255)
     gcCAGCTCCAGCTGCGGTAGGGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCTGGCTGCCAGGGCa                                                                                                                                          <  6:379377/149‑1 (MQ=255)
      ccagctccagctGGGGTAGTGCCCATACTCCCGAGCGGGCGCAGGGTAAGCACGGGCGTCGAAGGTGGGGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCGGCTGGCCACATACGGGTTGCTGCCCTGGCTGGCTGCCAGGGCag                                                                                                                                         <  8:228590/149‑2 (MQ=255)
      ccagctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCaa                                                                                                                                         <  5:177904/149‑1 (MQ=255)
        agctccagctGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATg                                                                                                                                       >  6:166741/1‑149 (MQ=255)
                     gTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCa                                                                                                                          <  7:70072/149‑1 (MQ=255)
                      tAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAg                                                                                                                         <  7:202111/149‑1 (MQ=255)
                            ccATAATCACGAGCGGGCGCAGGGTAAGGACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGcatga                                                                                                                   >  1:224638/1‑146 (MQ=255)
                            ccATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCacaa                                                                                                                   >  6:349866/1‑149 (MQ=255)
                               tAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAg                                                                                                                >  1:355485/1‑149 (MQ=255)
                                 aTCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGAc                                                                                                              <  7:216634/149‑1 (MQ=255)
                                  tCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACg                                                                                                             >  7:240675/1‑149 (MQ=255)
                                  tCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACg                                                                                                             <  2:101367/149‑1 (MQ=255)
                                      gAGCGGGCGCAGGGTAAGCACTGGCGTCGACGGTGGGGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTAc                                                                                                         <  3:58436/149‑1 (MQ=255)
                                         cGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGc                                                                                                      <  8:240675/149‑1 (MQ=255)
                                         cGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGc                                                                                                      <  6:159644/149‑1 (MQ=255)
                                               cAGGGTAAGCACTGGCGGCGAAGGTGGGGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTAt                                                                                                <  8:255125/149‑1 (MQ=255)
                                                  ggTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTtc                                                                                             >  1:175757/1‑149 (MQ=255)
                                                      aGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCACTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTccc                                                                                         >  7:249594/1‑149 (MQ=255)
                                                        cACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcgg                                                                                       >  2:247140/1‑149 (MQ=255)
                                                               gTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACATCCGGTACGGCATGTAGTTCTCCcggcgcggcg                                                                                >  1:133331/1‑149 (MQ=255)
                                                               gTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcgcggcg                                                                                <  5:285591/149‑1 (MQ=255)
                                                               gTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCcggcgcggcg                                                                                >  1:133330/1‑149 (MQ=255)
                                                                tCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGcggcgg                                                                               <  5:166741/149‑1 (MQ=255)
                                                                  gAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGAt                                                                             >  4:35073/1‑149 (MQ=255)
                                                                  gAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGAt                                                                             >  3:30357/1‑149 (MQ=255)
                                                                   aaGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCacaac                                                                                                                  <  1:194346/111‑1 (MQ=255)
                                                                   aaGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCacaac                                                                                                                  >  2:194346/1‑111 (MQ=255)
                                                                            gccgccTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggc                                                                   >  2:277231/1‑149 (MQ=255)
                                                                              cgccTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGggcgg                                                                 >  7:215476/1‑149 (MQ=255)
                                                                                  tGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGAGGATTAGCGGGGGCGGCaa                                                              >  4:382438/1‑148 (MQ=255)
                                                                                        ggggCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGt                                                       <  4:180593/149‑1 (MQ=255)
                                                                                              ggtggtAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGt                                                 <  2:175757/149‑1 (MQ=255)
                                                                                                   tAACGGGCGGCTGCGTGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACGCAGTGCAGGTAGCgg                                            <  8:242962/149‑1 (MQ=255)
                                                                                                    aaCGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGa                                                                              <  8:273718/114‑1 (MQ=255)
                                                                                                    aaCGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGa                                                                              >  7:273718/1‑114 (MQ=255)
                                                                                                    aaCGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGc                                           >  3:84243/1‑149 (MQ=255)
                                                                                                          cggcTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGTGGCCAAGcc                                     <  2:133330/149‑1 (MQ=255)
                                                                                                          cggagtctgGCCACATACGGGTGGCTGCCCTGGCAGCCAGACAGGGCAATGGGCAGCAGGGCCAGTCACAACAGACGGTACGGAATGTATTTCTCCCGGCGCGGCGGATTAGATGGGGCGGCAAACCCAGTGCAGGTAGTGTCCAAGcc                                     <  2:133331/143‑1 (MQ=255)
                                                                                                           ggcTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGccc                                    >  6:30722/1‑149 (MQ=255)
                                                                                                              tgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGc                                 >  7:5863/1‑149 (MQ=255)
                                                                                                              tgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGc                                 >  1:60609/1‑149 (MQ=255)
                                                                                                              tgctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACCGTACGGCATGTATTTCTCCCGGCGCGGCGCATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGACCGGc                                 >  6:196974/1‑149 (MQ=255)
                                                                                                               gctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGc                                 >  2:23050/1‑148 (MQ=255)
                                                                                                                ctgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTACTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCg                                >  4:100840/1‑148 (MQ=255)
                                                                                                                 tgGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCg                                >  1:387277/1‑147 (MQ=255)
                                                                                                                   gCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGaa                              <  3:35073/147‑1 (MQ=255)
                                                                                                                    ccACATACGGGTTGCTGCACTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggt                           <  8:249594/149‑1 (MQ=255)
                                                                                                                     cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtg                          >  7:129486/1‑149 (MQ=255)
                                                                                                                     cacaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtg                          >  8:126276/1‑149 (MQ=255)
                                                                                                                      acaTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTACTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtgg                         <  3:100840/149‑1 (MQ=255)
                                                                                                                       caTACGTGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggg                        <  6:258562/149‑1 (MQ=255)
                                                                                                                       caTACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAggtggg                        <  5:349866/149‑1 (MQ=255)
                                                                                                                              gTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCTAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGa                 >  7:306633/1‑149 (MQ=255)
                                                                                                                              gTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGc                   >  3:158629/1‑147 (MQ=255)
                                                                                                                              cccattgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGa                 >  7:249029/6‑149 (MQ=255)
                                                                                                                                 gctgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAACCCGGCGAAGGTGGGGTGGCGACg               <  2:355485/148‑1 (MQ=255)
                                                                                                                                 attgcCCTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACgg              >  4:17153/3‑149 (MQ=255)
                                                                                                                                   tgcCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCAAAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTg            >  1:273837/1‑149 (MQ=255)
                                                                                                                                     cccTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTgg           <  8:215476/148‑1 (MQ=255)
                                                                                                                                      ccTGGCTGGCTGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGc         >  2:32577/1‑149 (MQ=255)
                                                                                                                                       cTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGc         >  5:382937/1‑148 (MQ=255)
                                                                                                                                        tGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCATAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGc         >  4:382931/1‑147 (MQ=255)
                                                                                                                                        tGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGc                                      <  7:85482/118‑1 (MQ=255)
                                                                                                                                        tGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGc                                      >  8:85482/1‑118 (MQ=255)
                                                                                                                                          gCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGt     <  2:273837/149‑1 (MQ=255)
                                                                                                                                          gCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGGGGGGTGGCGACGGTGGGCCAGt     <  4:30357/149‑1 (MQ=255)
                                                                                                                                            agccagccAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTc   <  3:382438/149‑1 (MQ=255)
                                                                                                                                             gccagccaGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTcc  <  6:382937/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GTTGCGCCAGCTCCAGCTGCGGTAGTGCCCATAATCACGAGCGGGCGCAGGGTAAGCACTGGCGTCGAAGGTGGTGGCCGCCTGGGCGGGGGCCGGTGGTAACGGGCGGCTGCTGGCCACATACGGGTTGCTGCCCTGGCAGCCAGCCAGGGCAATGGGCAGCAGGGCCAGGCACAACAGACGGTACGGCATGTATTTCTCCCGGCGCGGCGGATTAGCGGGGGCGGCAAACCCAGTGCAGGTAGCGGCCAAGCCCGGCGAAGGTGGGGTGGCGACGGTGGGCCAGTTCC  >  NC_002947/675447‑675736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑