Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 704,122 T→A 5.3% L72H (CTC→CAC)  PP_5745 → paraquat‑inducible protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947704,1220TA 5.3% 234.8 / 2.8 76L72H (CTC→CAC) PP_5745paraquat‑inducible protein A
Reads supporting (aligned to +/- strand):  ref base T (37/35);  new base A (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

AGCACTTCGCAAACCTGCACACGTTGCGGCGCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGT  >  NC_002947/703977‑704269
                                                                                                                                                  |                                                                                                                                                   
aGCACTTCGCAAACCTGCACACGTTGCGGCGCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCgg                                                                                                                                                  >  5:359917/1‑149 (MQ=255)
        gCAAACCTGCACACGTTGCGGCGCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAggg                                                                                                                                            >  5:113984/1‑147 (MQ=255)
        gCAAACCTGCACACGTTGCGGCGCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGAGGGTACTCACGGTCatgatc                                                                                                                                          <  5:69287/149‑6 (MQ=255)
                gCACACGTTGCGGCGCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGa                                                                                                                                   >  6:304857/1‑148 (MQ=255)
                   cacGTTGCGGCGCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACAcc                                                                                                                               <  1:78940/149‑1 (MQ=255)
                    acGTTGCGGCGCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACca                                                                                                                              <  6:80363/149‑1 (MQ=255)
                    acGTTGCGGCGCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACca                                                                                                                              <  5:105327/149‑1 (MQ=255)
                      gTTGCGGCGCTATAGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCAAACATACTGCCGATCAT‑GACCGTGCGTACCCTAGGTCAGGGTAGCCCGGACACcat                                                                                                                             >  1:57982/1‑148 (MQ=255)
                           ggcgCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTTATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTTAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTc                                                                                                                       <  1:360377/149‑1 (MQ=255)
                             cgcTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCg                                                                                                                     <  6:113984/149‑1 (MQ=255)
                               cTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGCTCATAGACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACATGATGGTGTCCgg                                                                                                                    <  1:289214/149‑1 (MQ=255)
                               cTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGgt                                                                                                                   <  7:170376/149‑1 (MQ=255)
                               cTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGgt                                                                                                                   <  6:359917/149‑1 (MQ=255)
                                tATCGTTCATGCACGCCGGCCGAAGAGTATCGTGCGCACGTGGGCGTTTCTGATTGCGGCGTCGATCCTCTACTTCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGTTCAGGGTAGACCGGACACCATCATGTCCGgtg                                                                                                                  <  5:119866/149‑1 (MQ=255)
                                tATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGgtg                                                                                                                  >  3:60067/1‑149 (MQ=255)
                                tATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGCCCGgtg                                                                                                                  >  3:376277/1‑149 (MQ=255)
                                tATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCAGGCCAACATACTGCCGATCAT‑TACCGTGAGTACCCTCGGTCAGGGTAGACCGGACACCATCATGTCAGgtg                                                                                                                  >  3:46804/1‑149 (MQ=255)
                                    gTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGtcat                                                                                                              <  7:84236/149‑1 (MQ=255)
                                    gTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGtca                                                                                                               >  4:74064/1‑148 (MQ=255)
                                     tgcatgcaCGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGtcatc                                                                                                             <  2:298712/149‑1 (MQ=255)
                                       catgcaCGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAg                                                                                                                                              <  4:248397/114‑1 (MQ=255)
                                       catgcaCGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAg                                                                                                                                              >  3:248397/1‑114 (MQ=255)
                                        atgcaCGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCAcc                                                                                                          <  4:232433/149‑1 (MQ=255)
                                          gcaCGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCt                                                                                                        >  4:354257/1‑149 (MQ=255)
                                            aCGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGgt                                                                                                                   >  4:99933/1‑136 (MQ=255)
                                            aCGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGgt                                                                                                                   <  3:99933/136‑1 (MQ=255)
                                            aCGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGc                                                                                                      >  2:72214/1‑149 (MQ=255)
                                            aCGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGATGATTGCGGGGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGc                                                                                                      <  3:374694/149‑1 (MQ=255)
                                              gccggccgAACAGTATAGTGCGGACCTGGGCGTTGCTGATTGCTGCGTCTATCCTCTACATGCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCTGTCAGGGTAGGCCGGACACCATCATGTCCGGTGTCATCTCCCTGCTc                                                                                                    <  4:323548/149‑1 (MQ=255)
                                                   ccgAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCa                                                                                               >  7:357601/1‑149 (MQ=255)
                                                     gAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACg                                                                                             <  4:60067/149‑1 (MQ=255)
                                                          gTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGCTGTCATCACCCTGCTCAAGAACGGCATg                                                                                        >  1:125211/1‑149 (MQ=255)
                                                             tCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCTTCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTg                                                                                     >  5:333897/1‑149 (MQ=255)
                                                                    cagcTGGGCGGTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTg                                                                              <  4:46804/146‑1 (MQ=255)
                                                                         gggCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCt                                                                         >  4:384817/1‑149 (MQ=255)
                                                                            cGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCtgtg                                                                      <  3:384817/149‑1 (MQ=255)
                                                                                  tGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGtt                                                                 >  1:376219/1‑148 (MQ=255)
                                                                                    aTTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCAt                                                              >  3:48910/1‑149 (MQ=255)
                                                                                     ttGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCAtt                                                             <  3:74064/149‑1 (MQ=255)
                                                                                       gcggcgTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGGTGTGGTGTTCATTg                                                            >  4:168580/1‑148 (MQ=255)
                                                                                         ggcgTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCa                                                         <  7:249895/149‑1 (MQ=255)
                                                                                          gcggcgATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAg                                                        <  5:200156/149‑1 (MQ=255)
                                                                                               gATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATcc                                                   <  8:357601/149‑1 (MQ=255)
                                                                                                aTCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCt                                                  >  5:284525/1‑149 (MQ=255)
                                                                                                   ctctACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGt                                               >  3:351874/1‑149 (MQ=255)
                                                                                                    tctACATCCCGGCCAAAATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTggac                                              >  5:226469/1‑147 (MQ=255)
                                                                                                      tACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCAAGTATCCTGGTccc                                            >  4:247087/1‑149 (MQ=255)
                                                                                                           cccGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGt                                       >  3:107776/1‑149 (MQ=255)
                                                                                                           cccGGCCAACATACTGCCGATCAT‑GACCGTCAGTACCCTCCGTCAGGGTAGCCCGGACACCATCATCTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCTGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGt                                       <  2:57982/149‑1 (MQ=255)
                                                                                                            ccGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGtt                                      <  2:251488/149‑1 (MQ=255)
                                                                                                                  aaCATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCt                                >  1:7612/1‑149 (MQ=255)
                                                                                                                    cATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCtgg                              >  2:151568/1‑149 (MQ=255)
                                                                                                                    cATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCtgg                              >  2:127679/1‑149 (MQ=255)
                                                                                                                    cATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCtgg                              >  6:196049/1‑149 (MQ=255)
                                                                                                                       aCTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCtggtgg                           >  2:56724/1‑149 (MQ=255)
                                                                                                                        cTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGt                                                                                      <  7:278121/89‑1 (MQ=255)
                                                                                                                        cTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGt                                                                                      >  8:278121/1‑89 (MQ=255)
                                                                                                                         tGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTggg                          >  2:112730/1‑148 (MQ=255)
                                                                                                                           ccGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCAt                       >  2:26096/1‑149 (MQ=255)
                                                                                                                           ccGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGGTCAAGCTGGTGGGCAt                       >  6:263127/1‑149 (MQ=255)
                                                                                                                             gATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGATACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCg                     >  5:83122/1‑149 (MQ=255)
                                                                                                                               tCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGACCCCACGTTCAAGCTGGTGGGCATCGGc                   <  6:226469/149‑1 (MQ=255)
                                                                                                                                 aT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCt                  <  3:354257/148‑1 (MQ=255)
                                                                                                                                  t‑GACCGAGGGTACTCACGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTg                >  5:42272/1‑149 (MQ=255)
                                                                                                                                  t‑GACCGAGGGTACTCACGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTg                >  3:162366/1‑149 (MQ=255)
                                                                                                                                     aCCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGc               <  6:284525/148‑1 (MQ=255)
                                                                                                                                        gagggTACTCACGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCtgtt           <  6:42272/145‑1 (MQ=255)
                                                                                                                                        gTGAGTACCCTCGGTCAGGGTAGCCCGGATACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCtgtt           <  6:83122/149‑1 (MQ=255)
                                                                                                                                        gTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCtgtt           <  5:304857/149‑1 (MQ=255)
                                                                                                                                         tGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCa                                                         <  6:121354/102‑1 (MQ=255)
                                                                                                                                         tGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCa                                                         >  5:121354/1‑102 (MQ=255)
                                                                                                                                         tGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCtgttg          >  4:388787/1‑149 (MQ=255)
                                                                                                                                         tGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCtgttg          <  3:168580/149‑1 (MQ=255)
                                                                                                                                         tGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCtgttg          <  2:376219/149‑1 (MQ=255)
                                                                                                                                               cccTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTcc    >  8:370452/1‑149 (MQ=255)
                                                                                                                                                 cTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGt  <  4:48910/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGCACTTCGCAAACCTGCACACGTTGCGGCGCTATCGTGCATGCACGCCGGCCGAACAGTATCGTGCGCACCTGGGCGTTGCTGATTGCGGCGTCGATCCTCTACATCCCGGCCAACATACTGCCGATCAT‑GACCGTGAGTACCCTCGGTCAGGGTAGCCCGGACACCATCATGTCCGGTGTCATCACCCTGCTCAAGCACGGCATGGTGCCCATTGCGGCTGTGGTGTTCATTGCCAGTATCCTGGTCCCCACGTTCAAGCTGGTGGGCATCGGCTTGCTGTTGTACTCCGT  >  NC_002947/703977‑704269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑