Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 735,618 C→T 9.6% intergenic (‑44/+89) PP_0627 ← / ← PP_0628 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947735,6180CT 9.6% 172.6 / 6.7 62intergenic (‑44/+89)PP_0627/PP_0628hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (31/25);  new base T (3/3);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

AGGAGCAGCCACGGCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAA  >  NC_002947/735471‑735763
                                                                                                                                                   |                                                                                                                                                 
aGGAGCAGCCACGGCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACa                                                                                                                                                  <  5:136163/149‑1 (MQ=255)
        ccACGGCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTcgc                                                                                                                                          <  2:257736/149‑1 (MQ=255)
        ccACGGCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTcgc                                                                                                                                          >  7:7942/1‑149 (MQ=255)
          aCGGCCGGCAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCCTCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCGAACGCTGTAGCGTTTgcgc                                                                                                                                        <  2:354493/149‑1 (MQ=255)
            ggCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAg                                                                                                                                      <  2:11533/149‑1 (MQ=255)
            ggCAGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCGAACGCTGTAGCGTTTGCGCAg                                                                                                                                      <  4:217101/149‑1 (MQ=255)
               cGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGgcg                                                                                                                                   >  7:216277/1‑149 (MQ=255)
                ggAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGgcgc                                                                                                                                  <  4:85116/149‑1 (MQ=255)
                  aaGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGCCACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCtt                                                                                                                                <  7:313430/149‑1 (MQ=255)
                       ccTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGaaa                                                                                                                            >  5:205432/1‑148 (MQ=255)
                        cTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACa                                                                                                                          <  1:54647/149‑1 (MQ=255)
                                gACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACAGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGAt                                                                                                                    <  4:82393/147‑1 (MQ=255)
                                gACTTGGTGCTGGGGGGCTCGCAGATACCCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATaa                                                                                                                  >  5:82979/1‑149 (MQ=255)
                                 aCTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAAt                                                                                                                 <  8:216277/149‑1 (MQ=255)
                                       tGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTc                                                                                                           >  8:243063/1‑149 (MQ=255)
                                         cTGGGGGGCTAGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGTGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCCATTGAAACAAATGATAATGGTTTCt                                                                                                          >  3:305664/1‑148 (MQ=255)
                                              gggCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCt                                                                                                    <  6:148774/149‑1 (MQ=255)
                                                  tCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGgcg                                                                                                >  4:186246/1‑149 (MQ=255)
                                                    gCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGcc                                                                                              >  5:75154/1‑149 (MQ=255)
                                                      aGATACTCATCTACAAGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGa                                                                                            <  7:322566/149‑1 (MQ=255)
                                                          aCTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAgtg                                                                                         >  7:219545/1‑148 (MQ=255)
                                                              aTCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGc                                                                                    >  2:70277/1‑149 (MQ=255)
                                                                   cacGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCg                                                                               <  6:82979/149‑1 (MQ=255)
                                                                    acgacgCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCg                                                                               >  8:217994/1‑148 (MQ=255)
                                                                       acgCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCaaa                                                                           <  1:251176/149‑1 (MQ=255)
                                                                         gCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCaaa                                                                           >  3:370117/1‑147 (MQ=255)
                                                                              tCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGc                                                                    >  8:15951/1‑149 (MQ=255)
                                                                                ttCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTg                                                                  >  4:221857/1‑149 (MQ=255)
                                                                                 tCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCGAACGCTGTAGCGTTtgcg                                                                                                                                         >  5:332129/1‑77 (MQ=38)
                                                                                 tCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCGAACGCTGTAGCGTTtgcg                                                                                                                                         <  6:332129/77‑1 (MQ=38)
                                                                                  ccGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCt                                                                   <  5:182851/146‑1 (MQ=255)
                                                                                  ccGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCt                                                                   <  4:298911/146‑1 (MQ=255)
                                                                                   cGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCAc                                                               <  6:75154/149‑1 (MQ=255)
                                                                                          aGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGAt                                                                                                                    >  7:27912/1‑89 (MQ=255)
                                                                                          aGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGAt                                                                                                                    <  8:27912/89‑1 (MQ=255)
                                                                                            gCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCa                                                        >  4:141838/1‑147 (MQ=255)
                                                                                              gACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGa                                                    >  8:261028/1‑149 (MQ=255)
                                                                                              gACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGa                                                    <  8:7942/149‑1 (MQ=255)
                                                                                                    tCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATg                                              >  6:164707/1‑149 (MQ=255)
                                                                                                    tCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATg                                              >  2:39466/1‑149 (MQ=255)
                                                                                                            ggCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTc                                      >  5:210970/1‑149 (MQ=255)
                                                                                                                  gTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGa                                 <  7:217994/148‑1 (MQ=255)
                                                                                                                       gTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGAtgc                           >  2:81845/1‑149 (MQ=255)
                                                                                                                       gTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGAtgc                           >  4:291666/1‑149 (MQ=255)
                                                                                                                       gTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGAtgc                           >  5:126277/1‑149 (MQ=255)
                                                                                                                         aGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGAtgctg                         >  5:220787/1‑149 (MQ=255)
                                                                                                                          gACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGAtgctgc                        <  8:219545/149‑1 (MQ=255)
                                                                                                                                    ccTGCGCGAACGCTGTAGCGTTTGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGtt              >  7:256821/2‑149 (MQ=255)
                                                                                                                                    aCTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGtt              <  3:141838/149‑1 (MQ=255)
                                                                                                                                    aCTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGtt              >  3:226653/1‑149 (MQ=255)
                                                                                                                                    aCTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGtt              >  7:70236/1‑149 (MQ=255)
                                                                                                                                    aCTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGtt              >  7:64817/1‑149 (MQ=255)
                                                                                                                                     cTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTg             <  7:15951/149‑1 (MQ=255)
                                                                                                                                           aaaCGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCACAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCcgcg        >  8:123954/1‑148 (MQ=255)
                                                                                                                                           aaaCGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCATTCTATCACTTGCGACGAGTGTAATCAATCGCAAAAAGTCCCTGCACTAGAAAAAAGACTGATGCCTAAGTCGAAGAAGATGATCCTGAACAGGTTGCcgag        >  4:134152/1‑146 (MQ=255)
                                                                                                                                            aaCGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGGCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCa      <  6:205432/149‑1 (MQ=255)
                                                                                                                                             aCGCTGTAGCGTTTGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTTTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAg     >  7:252080/1‑149 (MQ=255)
                                                                                                                                               gCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGATTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAATACTGATGCCTACGTCGAATACGATACTGCGGCGCAGGTTGCCTCGCAgg    >  8:345008/1‑148 (MQ=255)
                                                                                                                                                cTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAggta  <  5:164707/149‑3 (MQ=255)
                                                                                                                                                cTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGaa  <  1:39466/149‑1 (MQ=255)
                                                                                                                                                 tACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGccc                   <  8:227064/131‑1 (MQ=255)
                                                                                                                                                 tACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGccc                   >  7:227064/1‑131 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGGAGCAGCCACGGCCGGAAGGGCCTGCGCTCGACTTGGTGCTGGGGGGCTCGCAGATACTCATCGACACGACGCTGATCTTCCGGGTTCAGGCGACTGGTCATGGTGGGCCTCGTCAGGTAGACGTTTGTGACTGCGCAAACGCTACAGCGTTCGCGCAGGCGCTTGAAACAAATGATAATGCTTTCTATCCCTGGCGCCGAGTGTACGCCATCGCAAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAA  >  NC_002947/735471‑735763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑