Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	82,854	A→C	8.2%	L92R (CTG→CGG)	qor‑I ←	quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	82,854	0	A	C	8.2%	178.6 / 5.9	61	L92R (CTG→CGG)	qor‑I	quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (25/31);  new base C (2/3);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

ACCCGCCTTCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAGTAGGTATCGAT  >  NC_002947/82706‑83002                                                                                                                                                     |                                                                                                                                                      ccccGCCTTCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCa                                                                                                                                                       >  7:252421/2‑149 (MQ=255)       cTTCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGgc                                                                                                                                                 <  7:4361/149‑1 (MQ=255)       cTTCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGgc                                                                                                                                                 >  7:289183/1‑149 (MQ=255)        ttCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGgcc                                                                                                                                                >  1:174968/1‑149 (MQ=255)             cTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGGCCCGCTGggcgccta                                                                                                                                           <  1:336063/149‑3 (MQ=255)                   ggTCTGATTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCCCCGCTGCCCCCTGCTCGAAGCTGATGGTGGCCGGCAGCGTGCCCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTCTAGGCGCCCAGCGCGCCGCCGGCAtg‑tg                                                                                                                                    >  2:336409/1‑149 (MQ=255)                     tCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGcc                                                                                                                                  >  6:204779/1‑149 (MQ=255)                     tCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGc                                                                                                                                   <  7:317816/148‑1 (MQ=255)                         cTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCcaca                                                                                                                               <  3:186508/148‑1 (MQ=255)                         cTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCcac                                                                                                                                <  5:336376/147‑1 (MQ=255)                         cTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCacac                                                                                                                              >  1:46174/1‑149 (MQ=255)                            cagcagGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACg                                                                                                                             <  1:63083/147‑1 (MQ=255)                             agcagGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGc                                                                                                                                   <  4:109321/140‑1 (MQ=255)                             agcagGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGc                                                                                                                                   >  3:109321/1‑140 (MQ=255)                                agGTACTGTACGGTCAAACCCTTGATCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTcc                                                                                                                       <  4:256423/149‑1 (MQ=255)                                  gTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCACGCTGTAGGCGGCCCGCTGggcgcctaca                                                                                                                                         >  7:20307/1‑126 (MQ=255)                                  gTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCACGCTGTAGGCGGCCCGCTGggcgcctaca                                                                                                                                         <  8:20307/130‑5 (MQ=255)                                     cTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCt                                                                                                                  <  5:71998/149‑1 (MQ=255)                                       gTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCtt                                                                                                                 >  3:274411/1‑148 (MQ=255)                                        tACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCg                                                                                                                                                    >  6:188922/1‑113 (MQ=255)                                          cGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAg                                                                                                              <  4:274411/148‑1 (MQ=255)                                            ggccaaCCCTTGTGCATCACCGCTGCCGCCTGCTCGATGCTGATGGTGTCCTGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCg                                                                                                           <  6:257538/145‑1 (MQ=255)                                             tCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCgg                                                                                                          >  3:385968/1‑149 (MQ=255)                                                 aCCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGaca                                                                                                      <  2:304816/149‑1 (MQ=255)                                                            tCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCAtg‑tg                                                                                                                                    <  5:76897/108‑1 (MQ=255)                                                            tCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCAtg‑tg                                                                                                                                    >  6:76897/1‑108 (MQ=255)                                                              aCCGCTGCCGCCTGCTTGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATGTTGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCa                                                                                          <  1:81235/149‑1 (MQ=255)                                                              aCCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCa                                                                                          <  8:129959/148‑1 (MQ=255)                                                                     ccgccTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCctc                                                                                   <  4:385968/148‑1 (MQ=255)                                                                               gAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCAAACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTg                                                                        >  1:230778/1‑149 (MQ=255)                                                                                 aGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGGCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGcc                                                                      <  8:252421/149‑1 (MQ=255)                                                                                 aGCTGATGGTGTCCGGAAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGcc                                                                      <  8:252418/149‑1 (MQ=255)                                                                                    tGATGGTGTCCGGCAGATTGACCAGGTTGGCTTCCGGTAGCTTATGCACCTCGCTGTAGGCGCCCAGCGGGACGCCGTCATG‑TGCGACACTGTCTCCTACCTTCAGCCGGTAAACGCCTTc                                                                                               <  8:284505/121‑1 (MQ=255)                                                                                     gATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCtt                                                                  >  4:155283/1‑149 (MQ=255)                                                                                      aTGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCtt                                                                  <  2:174968/148‑1 (MQ=255)                                                                                          tgtCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTa                                                             <  5:204779/149‑1 (MQ=255)                                                                                           gtCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACcaca                                                                            >  2:178125/1‑133 (MQ=255)                                                                                           gtCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACcaca                                                                            <  1:178125/133‑1 (MQ=255)                                                                                                    cTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGc                                                      >  5:208453/1‑146 (MQ=255)                                                                                                    cTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGc                                                      >  4:86981/1‑146 (MQ=255)                                                                                                      tGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCCGCATG‑TGCCACACGCTATCCTACATTCAGCCGGGACACGCCTTCCCCCACGCCCTCTAACACACCTGCCGCTGCGCTACCCAGGCCac                                                   >  8:274095/1‑146 (MQ=255)                                                                                                              ttGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGa                                         <  2:230778/149‑1 (MQ=255)                                                                                                                   ttCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGAcggcg                                    >  8:202763/1‑149 (MQ=255)                                                                                                                      cGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACggcggc                                  <  2:46174/148‑1 (MQ=255)                                                                                                                      cGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACggcggc                                  <  7:202763/148‑1 (MQ=255)                                                                                                                       ggTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTAccc                                                          >  4:286236/1‑123 (MQ=255)                                                                                                                       ggTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTAccc                                                          <  3:286236/123‑1 (MQ=255)                                                                                                                                  cACCCCGCTGTAGGCGCCCAGCGGGCCGCCTGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCg                      <  2:384382/148‑1 (MQ=255)                                                                                                                                   aCCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGcgaggt                    >  6:326481/1‑144 (MQ=255)                                                                                                                                    ccTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCtt                   <  6:208453/149‑1 (MQ=255)                                                                                                                                    ccTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCtt                   >  4:143355/1‑149 (MQ=255)                                                                                                                                     cTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTc                  >  6:274945/1‑149 (MQ=255)                                                                                                                                     cTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTc                  >  1:19943/1‑149 (MQ=255)                                                                                                                                     cTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTc                  >  3:354740/1‑149 (MQ=255)                                                                                                                                        gCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCgg                <  7:78881/148‑1 (MQ=255)                                                                                                                                        gCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCgg                <  2:19943/148‑1 (MQ=255)                                                                                                                                         cTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGaa              <  8:289183/149‑1 (MQ=255)                                                                                                                                          tGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAg             >  5:106013/1‑149 (MQ=255)                                                                                                                                            tAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAGt            <  3:86981/148‑1 (MQ=255)                                                                                                                                             cggcggcCCGCTGGGCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAg                          >  2:41530/2‑133 (MQ=255)                                                                                                                                             cggcggcCCGCTGGGCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAg                          <  1:41530/132‑1 (MQ=255)                                                                                                                                               gcgcCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAGTAgg         >  4:282926/1‑148 (MQ=255)                                                                                                                                                     aGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGTCGCATACAGCCCGCTTCGGAAGTAGGTATCGAt  <  4:354740/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      ACCCGCCTTCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAGTAGGTATCGAT  >  NC_002947/82706‑83002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑