Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 794,514 G→T 6.0% R219L (CGC→CTC)  PP_0680 → ATP‑dependent protease‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947794,5140GT 6.0% 214.1 / 2.2 66R219L (CGC→CTC) PP_0680ATP‑dependent protease‑like protein
Reads supporting (aligned to +/- strand):  ref base G (34/28);  new base T (2/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

AAAAGGACGTGGCCTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGC  >  NC_002947/794367‑794662
                                                                                                                                                   |                                                                                                                                                    
aaaaGGACGTGGCCTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAgcgc                                                                                                                                                     <  1:24221/149‑1 (MQ=255)
 aaaGGACGTGGCCTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGcc                                                                                                                                                    >  2:120847/1‑149 (MQ=255)
     gACGTGGCCTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCaa                                                                                                                                                >  2:3117/1‑149 (MQ=255)
       cGTGGCCTTGTACCGCGATGCCAGCAACGTCGGCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACg                                                                                                                                              <  5:110977/149‑1 (MQ=255)
         tGGCCTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGaa                                                                                                                                            <  4:319197/149‑1 (MQ=255)
           gCCTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGCGCTCCTCaagcagg                                                                                                                                           <  7:147163/148‑6 (MQ=255)
           gCCTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAag                                                                                                                                           <  1:41339/148‑1 (MQ=255)
             cTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGTGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGc                                                                                                                                        >  4:8898/1‑149 (MQ=255)
             cTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGCGCTCCTCaagcagg                                                                                                                                           <  2:330345/146‑6 (MQ=255)
             cTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGCGCTCCTCaagcagg                                                                                                                                           >  1:330345/1‑141 (MQ=255)
                 tACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTgg                                                                                                                                     <  5:381472/148‑1 (MQ=255)
                 tACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGc                                                                                                                                    <  3:262034/149‑1 (MQ=255)
                   ccGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTgg                                                                                                                                     >  6:154507/1‑146 (MQ=255)
                   ccGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTgg                                                                                                                                     <  5:154507/146‑1 (MQ=255)
                    cgcgATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAg                                                                                                                                 >  2:361061/1‑149 (MQ=255)
                        aTGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGATGTATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGGGAGCAATTCCACGAGGTCATTGCCCTGCTTGAGGAGCGGCTCAACGAAGAGCTGGCCAGCGTg                                                                                                                             <  2:200653/149‑1 (MQ=255)
                            cAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCg                                                                                                                          >  6:190565/1‑148 (MQ=255)
                             aGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGc                                                                                                                         >  1:227180/1‑148 (MQ=255)
                              gCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCaa                                                                                                                       <  8:119070/149‑1 (MQ=255)
                               cAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAAt                                                                                                                      <  5:190565/149‑1 (MQ=255)
                               cAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAAt                                                                                                                      <  7:79754/149‑1 (MQ=255)
                               cAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAAt                                                                                                                      <  1:361061/149‑1 (MQ=255)
                                aaCGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGTGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATg                                                                                                                     <  3:8898/149‑1 (MQ=255)
                                      gCCTTGACGCCGATGGCTGATGGGAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGTGGTGCGCGAGCAATTCCACGATGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAgcg                                                                                                               <  7:345054/149‑1 (MQ=255)
                                      gCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGATTTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAgcg                                                                                                               <  2:58868/149‑1 (MQ=255)
                                      gCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAgc                                                                                                                <  1:354799/148‑1 (MQ=255)
                                        cTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAgcgc                                                                                                              >  8:49506/1‑148 (MQ=255)
                                          tCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGaa                                                                                                           >  5:155910/1‑149 (MQ=255)
                                               ccGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGaa                                                                                                      <  1:3117/149‑1 (MQ=255)
                                               ccGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGaa                                                                                                      <  1:120847/149‑1 (MQ=255)
                                                         aTGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCt                                                                                             <  3:369294/148‑1 (MQ=255)
                                                              aaGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTgcgc                                                                                         >  6:56534/1‑147 (MQ=255)
                                                                  cgcTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCATTGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCt                                                                                    >  6:205606/1‑148 (MQ=255)
                                                                       gATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACg                                                                               >  8:296019/1‑148 (MQ=255)
                                                                            aGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGaaa                                                                         <  7:296019/149‑1 (MQ=255)
                                                                             gCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCTCACAACCAGCTGCGCCAGCTCAACGAAGAAAc                                                                        >  4:26347/1‑149 (MQ=255)
                                                                                 aaTTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCAt                                                                     <  6:26712/148‑1 (MQ=255)
                                                                                 aaTTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATc                                                                    <  2:227180/149‑1 (MQ=255)
                                                                                   tttGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATccc                                                                  >  8:129771/1‑147 (MQ=255)
                                                                                   tttGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCAc                                                                  >  1:377155/1‑149 (MQ=255)
                                                                                        ccAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTgg                                                             >  6:25477/1‑149 (MQ=255)
                                                                                                cGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCACCGAAGAAACCATCACCCTGGCACTGCAg                                                     >  4:38077/1‑149 (MQ=255)
                                                                                                cGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAg                                                     <  8:140245/149‑1 (MQ=255)
                                                                                                  gaggagGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGcc                                                   >  5:13597/1‑149 (MQ=255)
                                                                                                  gaggagGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGcc                                                   >  3:123598/1‑149 (MQ=255)
                                                                                                     gaggTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGtt                                                >  2:7120/1‑149 (MQ=255)
                                                                                                           cgcgAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGcc                                                   <  1:263779/140‑1 (MQ=255)
                                                                                                           cgcgAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGcc                                                   >  2:263779/1‑140 (MQ=255)
                                                                                                           cgcgAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTgg                                           <  5:25477/148‑1 (MQ=255)
                                                                                                            gcgAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGgcg                                         >  3:336046/1‑149 (MQ=255)
                                                                                                            gcgAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGgcg                                         >  1:386009/1‑149 (MQ=255)
                                                                                                             cgAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCg                                                  <  8:129823/139‑1 (MQ=255)
                                                                                                             cgAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCg                                                  >  7:129823/1‑139 (MQ=255)
                                                                                                              gAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGcgcc                                       >  2:94502/1‑149 (MQ=255)
                                                                                                                    ttCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTc                                 >  5:181190/1‑149 (MQ=255)
                                                                                                                      ccACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCg                                <  4:336046/148‑1 (MQ=255)
                                                                                                                        aCGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGa                              >  2:208429/1‑148 (MQ=255)
                                                                                                                          gAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCCGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGaaaa                           <  7:227720/149‑1 (MQ=255)
                                                                                                                            gtaCATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGATTTCAAGCATGACGCAATTGAATCGCGAATCTAACAACCAGATGCGCCAGCTAAACGAAGAAACCATCAACCTTGCACTGCAGACGATGCTTGCGCCTCTGTCGGAAAAg                          >  1:307135/3‑148 (MQ=255)
                                                                                                                             gACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATCGAAGCGCGAATCGAACAACCAGCTGCGCGAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTa                        >  5:274699/1‑149 (MQ=255)
                                                                                                                               cATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCAAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACg                      >  6:263007/1‑149 (MQ=255)
                                                                                                                                   ctcttcgttGAGGCGCTCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCg                   >  7:226377/8‑148 (MQ=255)
                                                                                                                                     ccTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGaa                             >  4:50111/1‑136 (MQ=255)
                                                                                                                                     ccTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGaa                             <  3:50111/136‑1 (MQ=255)
                                                                                                                                     ccTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGaa                 >  2:265795/1‑148 (MQ=255)
                                                                                                                                       tGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAAc              >  5:262091/1‑149 (MQ=255)
                                                                                                                                                agCGCCTCAACCAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGGGAATCGAACAAACAGCTCCGCCAGCTCAACGAAGAACCCATCACACTCGCACTGCAGCCGTAGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGgtg     >  8:95400/1‑149 (MQ=255)
                                                                                                                                                   gcCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTgc  <  2:377155/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AAAAGGACGTGGCCTTGTACCGCGATGCCAGCAACGTCGCCTTCACGCCGATGGCTGATGGCAAGGCGCTGGATGAAGCTGAATTTGCCCAATTGCCGGAGGAGGTGCGCGAGCAATTCCACGAGGACATTGCCCTGCTTGAGGAGCGCCTCAACGAAGAGCTGGCCAGCCTGCCGCAATGGAAGCGCGAATCGAACAACCAGCTGCGCCAGCTCAACGAAGAAACCATCACCCTGGCACTGCAGCCGTTGCTGGCGCCGCTGTCGGAAAAGTACGCCGAAAACGCGGCGGTGTGC  >  NC_002947/794367‑794662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑