Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	82,857	G→T	8.2%	P91Q (CCG→CAG)	qor‑I ←	quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	82,857	0	G	T	8.2%	178.0 / 5.4	61	P91Q (CCG→CAG)	qor‑I	quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (24/32);  new base T (2/3);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

CTTCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAGTAGGTATCGAT  >  NC_002947/82712‑83002                                                                                                                                                  |                                                                                                                                                   cTTCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGgc                                                                                                                                                 >  7:289183/1‑149 (MQ=255) cTTCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGgc                                                                                                                                                 <  7:4361/149‑1 (MQ=255)  ttCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGgcc                                                                                                                                                >  1:174968/1‑149 (MQ=255)       cTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGGCCCGCTGggcgccta                                                                                                                                           <  1:336063/149‑3 (MQ=255)             ggTCTGATTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCCCCGCTGCCCCCTGCTCGAAGCTGATGGTGGCCGGCAGCGTGCCCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTCTAGGCGCCCAGCGCGCCGCCGGCAtg‑tg                                                                                                                                    >  2:336409/1‑149 (MQ=255)               tCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGcc                                                                                                                                  >  6:204779/1‑149 (MQ=255)               tCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGc                                                                                                                                   <  7:317816/148‑1 (MQ=255)                   cTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCcaca                                                                                                                               <  3:186508/148‑1 (MQ=255)                   cTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCcac                                                                                                                                <  5:336376/147‑1 (MQ=255)                   cTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCacac                                                                                                                              >  1:46174/1‑149 (MQ=255)                      cagcagGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACg                                                                                                                             <  1:63083/147‑1 (MQ=255)                       agcagGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGc                                                                                                                                   >  3:109321/1‑140 (MQ=255)                       agcagGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGc                                                                                                                                   <  4:109321/140‑1 (MQ=255)                          agGTACTGTACGGTCAAACCCTTGATCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTcc                                                                                                                       <  4:256423/149‑1 (MQ=255)                            gTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCACGCTGTAGGCGGCCCGCTGggcgcctaca                                                                                                                                         >  7:20307/1‑126 (MQ=255)                            gTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCACGCTGTAGGCGGCCCGCTGggcgcctaca                                                                                                                                         <  8:20307/130‑5 (MQ=255)                               cTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCt                                                                                                                  <  5:71998/149‑1 (MQ=255)                                 gTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCtt                                                                                                                 >  3:274411/1‑148 (MQ=255)                                  tACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCg                                                                                                                                                    >  6:188922/1‑113 (MQ=255)                                    cGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAg                                                                                                              <  4:274411/148‑1 (MQ=255)                                      ggccaaCCCTTGTGCATCACCGCTGCCGCCTGCTCGATGCTGATGGTGTCCTGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCg                                                                                                           <  6:257538/145‑1 (MQ=255)                                       tCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCgg                                                                                                          >  3:385968/1‑149 (MQ=255)                                           aCCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGaca                                                                                                      <  2:304816/149‑1 (MQ=255)                                                      tCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCAtg‑tg                                                                                                                                    <  5:76897/108‑1 (MQ=255)                                                      tCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCAtg‑tg                                                                                                                                    >  6:76897/1‑108 (MQ=255)                                                        aCCGCTGCCGCCTGCTTGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATGTTGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCa                                                                                          <  1:81235/149‑1 (MQ=255)                                                        aCCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCa                                                                                          <  8:129959/148‑1 (MQ=255)                                                               ccgccTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCctc                                                                                   <  4:385968/148‑1 (MQ=255)                                                                         gAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCAAACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTg                                                                        >  1:230778/1‑149 (MQ=255)                                                                           aGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGGCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGcc                                                                      <  8:252421/149‑1 (MQ=255)                                                                           aGCTGATGGTGTCCGGAAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGcc                                                                      <  8:252418/149‑1 (MQ=255)                                                                              tGATGGTGTCCGGCAGATTGACCAGGTTGGCTTCCGGTAGCTTATGCACCTCGCTGTAGGCGCCCAGCGGGACGCCGTCATG‑TGCGACACTGTCTCCTACCTTCAGCCGGTAAACGCCTTc                                                                                               <  8:284505/121‑1 (MQ=255)                                                                               gATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCtt                                                                  >  4:155283/1‑149 (MQ=255)                                                                                aTGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCtt                                                                  <  2:174968/148‑1 (MQ=255)                                                                                    tgtCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTa                                                             <  5:204779/149‑1 (MQ=255)                                                                                     gtCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACcaca                                                                            <  1:178125/133‑1 (MQ=255)                                                                                     gtCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACcaca                                                                            >  2:178125/1‑133 (MQ=255)                                                                                              cTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGc                                                      >  5:208453/1‑146 (MQ=255)                                                                                              cTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGc                                                      >  4:86981/1‑146 (MQ=255)                                                                                                tGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCCGCATG‑TGCCACACGCTATCCTACATTCAGCCGGGACACGCCTTCCCCCACGCCCTCTAACACACCTGCCGCTGCGCTACCCAGGCCac                                                   >  8:274095/1‑146 (MQ=255)                                                                                                        ttGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGa                                         <  2:230778/149‑1 (MQ=255)                                                                                                             ttCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGAcggcg                                    >  8:202763/1‑149 (MQ=255)                                                                                                                cGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACggcggc                                  <  7:202763/148‑1 (MQ=255)                                                                                                                cGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACggcggc                                  <  2:46174/148‑1 (MQ=255)                                                                                                                 ggTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTAccc                                                          >  4:286236/1‑123 (MQ=255)                                                                                                                 ggTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTAccc                                                          <  3:286236/123‑1 (MQ=255)                                                                                                                            cACCCCGCTGTAGGCGCCCAGCGGGCCGCCTGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCg                      <  2:384382/148‑1 (MQ=255)                                                                                                                             aCCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGcgaggt                    >  6:326481/1‑144 (MQ=255)                                                                                                                              ccTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCtt                   <  6:208453/149‑1 (MQ=255)                                                                                                                              ccTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCtt                   >  4:143355/1‑149 (MQ=255)                                                                                                                               cTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTc                  >  1:19943/1‑149 (MQ=255)                                                                                                                               cTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTc                  >  6:274945/1‑149 (MQ=255)                                                                                                                               cTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTc                  >  3:354740/1‑149 (MQ=255)                                                                                                                                  gCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCgg                <  7:78881/148‑1 (MQ=255)                                                                                                                                  gCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCgg                <  2:19943/148‑1 (MQ=255)                                                                                                                                   cTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGaa              <  8:289183/149‑1 (MQ=255)                                                                                                                                    tGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAg             >  5:106013/1‑149 (MQ=255)                                                                                                                                      tAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAGt            <  3:86981/148‑1 (MQ=255)                                                                                                                                       cggcggcCCGCTGGGCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAg                          >  2:41530/2‑133 (MQ=255)                                                                                                                                       cggcggcCCGCTGGGCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAg                          <  1:41530/132‑1 (MQ=255)                                                                                                                                         gcgcCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAGTAgg         >  4:282926/1‑148 (MQ=255)                                                                                                                                               aGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGTCGCATACAGCCCGCTTCGGAAGTAGGTATCGAt  <  4:354740/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CTTCACCTCATAGGTCTGCTTCAGCAGGTACTGTACGGTCAAACCCTTGAGCATCACCGCTGCCGCCTGCTCGAAGCTGATGGTGTCCGGCAGCTTGACCAGGTTGGCTTCCGGTAGCGTATGCACCTCGCTGTAGGCGCCCAGCGGGCCGCCGGCATG‑TGCCACACGGTCTCCTACCTTCAGCCGGGACACGCCTTCGCCCACGGCCTCTACCACACCTGCCGCTTCGGTACCCAGGCCAGAAGGCAGGGACGGCGGCGCATACAGCCCGCTTCGGAAGTAGGTATCGAT  >  NC_002947/82712‑83002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑