Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 927,092 A→G 5.2% I6177T (ATC→ACC)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947927,0920AG 5.2% 224.5 / 2.2 76I6177T (ATC→ACC) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (34/38);  new base G (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

GTACGCGCCCGGCCAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCGGTGAGGCCGGCGGCGCTGT  >  NC_002947/926946‑927239
                                                                                                                                                  |                                                                                                                                                   
gtACGCGCCCGGCCAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGAtg                                                                                                                                                   <  5:146795/149‑1 (MQ=255)
 tACGCGCCCGGCCAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGAtgt                                                                                                                                                  <  4:42921/149‑1 (MQ=255)
   cgcgCCCGGCCAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTgg                                                                                                                                                >  4:117187/1‑149 (MQ=255)
    gcgcCCGGCCAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCATCACCCTGCAGGGTGGTGCggg                                                                                                                                               <  2:172214/149‑2 (MQ=255)
    gcgcCCGGCCAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGc                                                                                                                                               >  6:252691/1‑149 (MQ=255)
      gcCCGGCCAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCa                                                                                                                                             >  6:232100/1‑149 (MQ=255)
          ggCCAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGTCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCt                                                                                                                                         >  3:62209/1‑149 (MQ=255)
            ccAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTgg                                                                                                                                       <  5:252691/149‑1 (MQ=255)
             cAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGc                                                                                                                                      <  8:122538/149‑1 (MQ=255)
               ggTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGTCACCCTGGCTg                                                                                                                                    <  1:225481/149‑1 (MQ=255)
                gTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGc                                                                                                                                   >  2:339961/1‑149 (MQ=255)
                     ccAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCGTCg                                                                                                                              >  8:149965/1‑149 (MQ=255)
                     ccAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATc                                                                                                                               >  2:602/1‑148 (MQ=255)
                     ccAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATc                                                                                                                               >  6:143292/1‑148 (MQ=255)
                     ccAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATc                                                                                                                               >  7:212853/1‑148 (MQ=255)
                     ccAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCACATCACCCTGCAGGGTGGTGCgg                                                                                                                                                <  3:375823/131‑1 (MQ=255)
                     ccAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCACATCACCCTGCAGGGTGGTGCgg                                                                                                                                                >  4:375823/1‑131 (MQ=255)
                     ccAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCAGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATc                                                                                                                               >  1:125562/1‑148 (MQ=255)
                      cAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATc                                                                                                                               >  8:115345/1‑147 (MQ=255)
                       aGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAg                                                                                                                            <  5:387271/149‑1 (MQ=255)
                          tCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAggtg                                                                                                                         <  2:236451/149‑1 (MQ=255)
                             aTGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTc                                                                                                                      <  5:11954/149‑1 (MQ=255)
                                cTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGtt                                                                                                                   <  5:280103/149‑1 (MQ=255)
                                  ggCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTg                                                                                                                                    >  6:105029/1‑130 (MQ=255)
                                  ggCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTg                                                                                                                                    <  5:105029/130‑1 (MQ=255)
                                   gCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGTCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGcc                                                                                                                <  4:62209/149‑1 (MQ=255)
                                   gCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGtt                                                                                                                   <  7:151184/146‑1 (MQ=255)
                                   gCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACCCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGAAGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGcc                                                                                                                >  4:359877/1‑149 (MQ=255)
                                        aCCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGc                                                                                                                                      <  8:280957/122‑1 (MQ=255)
                                        aCCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGc                                                                                                                                      <  6:157610/122‑1 (MQ=255)
                                           gccgccCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCATGGCTGCCATCTAGGTGGTCGTTGCCGTCAGTGc                                                                                                        >  7:12723/1‑149 (MQ=255)
                                            ccgccCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGGGGTCGTTGCCGTCAGTGcc                                                                                                       <  2:257706/149‑1 (MQ=255)
                                             cgccCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCg                                                                                                      >  7:316700/1‑149 (MQ=255)
                                               ccccACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGc                                                                                                    <  5:143292/149‑1 (MQ=255)
                                                cccACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGc                                                                                                    >  3:386382/1‑148 (MQ=255)
                                                 ccACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGa                                                                                                  >  1:198970/1‑149 (MQ=255)
                                                     agcagGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATca                                                                                               >  4:44449/1‑148 (MQ=255)
                                                       cagGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACAt                                                                                            <  7:115345/149‑1 (MQ=255)
                                                       cagGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACAt                                                                                            <  5:149901/149‑1 (MQ=255)
                                                         gggTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCg                                                                                          <  8:83952/149‑1 (MQ=255)
                                                         gggTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCg                                                                                          >  5:339991/1‑149 (MQ=255)
                                                          ggTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCgg                                                                                         >  3:68059/1‑149 (MQ=255)
                                                            tgtCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTg                                                                                       <  1:339961/149‑1 (MQ=255)
                                                                  ttCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTg                                                                                                                                        <  4:339962/94‑1 (MQ=255)
                                                                     ccGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGc                                                                               <  5:232100/148‑1 (MQ=255)
                                                                      cGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGc                                                                               >  3:223337/1‑147 (MQ=255)
                                                                           caacgacgacgaTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCACGGTGATGTGGCCACCCTGGCGGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATAACATCGGTGGCCGCTGCAAGGCTg                                                                        >  6:381950/7‑149 (MQ=255)
                                                                           cACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGGGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTg                                                                        >  7:212495/1‑149 (MQ=255)
                                                                                       tGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCcgcg                                                            >  8:284525/1‑149 (MQ=255)
                                                                                         gCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCcgcg                                                            <  1:602/147‑1 (MQ=255)
                                                                                         gCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGa                                                          <  1:327377/149‑1 (MQ=255)
                                                                                          cAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGAc                                                         >  3:286839/1‑149 (MQ=255)
                                                                                               ttCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACtcgtc                                                    <  7:29107/149‑1 (MQ=255)
                                                                                                    gTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCa                                                <  4:223337/148‑1 (MQ=255)
                                                                                                       gACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTAt                                            >  2:209636/1‑149 (MQ=255)
                                                                                                        accacccccAGCGTGTCGTCACCCGCACCACCCTTCAGGGTGATGTGGCCCCCCGGGCGGCCATCGAGGTGGGCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCGGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATc                                           <  5:302551/149‑1 (MQ=255)
                                                                                                           accaccaGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGc                                        <  2:316729/149‑1 (MQ=255)
                                                                                                           accaccaGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGc                                        <  3:277065/149‑1 (MQ=255)
                                                                                                           accaccaGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGc                                        <  2:198970/149‑1 (MQ=255)
                                                                                                              acccGCGTGTCGGCACCCGCACCACCCTGCCTGGGGATGTGGCCTCCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCAGGAGATCACATCGGTGGCCGCTGCCAGGGTGGCGAGTGCCGCGTTCTCGTCGCCAGTATCGGCcag                                     <  8:12723/149‑1 (MQ=255)
                                                                                                              accaGCGGGTCGTCACCCGCACCACCCTGCAGGGGTATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCcag                                     <  8:212495/149‑1 (MQ=255)
                                                                                                               ccaGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCcag                                     >  1:307235/1‑148 (MQ=255)
                                                                                                                       tcgtcACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACtcgtc                                                    >  3:100844/1‑125 (MQ=255)
                                                                                                                       tcgtcACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACtcgtc                                                    <  4:100844/125‑1 (MQ=255)
                                                                                                                             cccGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGc                      >  1:307382/1‑149 (MQ=255)
                                                                                                                              ccacatcaccCTGCAGGGTGGTGCGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCg                     >  6:355557/7‑149 (MQ=255)
                                                                                                                              ccGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCg                     >  7:279995/1‑149 (MQ=255)
                                                                                                                              ccGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCg                     >  2:30503/1‑149 (MQ=255)
                                                                                                                              ccGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCg                     <  4:286839/149‑1 (MQ=255)
                                                                                                                               cGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCgg                    >  3:319188/1‑149 (MQ=255)
                                                                                                                                   ccaccCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCGGTGAg                <  4:386382/149‑1 (MQ=255)
                                                                                                                                         tGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCGGTGAGGCcggc          >  2:261443/1‑149 (MQ=255)
                                                                                                                                           cAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCGGTGAGGCcggcgg        <  6:339991/149‑1 (MQ=255)
                                                                                                                                            aGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCGGTGAGGCCggcggc       <  3:117187/149‑1 (MQ=255)
                                                                                                                                             gggTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCGGTGAGGCCGgcggcg      <  8:212853/149‑1 (MQ=255)
                                                                                                                                              ggTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCGGTGAGGCCGGCGgcgc     >  8:111411/1‑149 (MQ=255)
                                                                                                                                                  aTGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCGGTGAGGCCGGCGGCGCTGt  <  8:279995/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTACGCGCCCGGCCAGGTTGCCCAGGTCAATGCTGGCATCACCGCCGCCCCACAGCAGGGTGTCGGTTCCCGAGCCACCGTCGACGCTGGCAAAGTTCTGGTCGACCACCACCAGCGTGTCGTCACCCGCACCACCCTGCAGGGTGATGTGGCCACCCTGGCTGCCATCGAGGTGGTCGTTGCCGTCAGTGCCGGCGATCACATCGGTGGCCGCTGCCAGGCTGGCGAGTGCCGCGGACTCGTCGCCAGTATCGGCCAGCAGCGATGTGGTGGCGGTGAGGCCGGCGGCGCTGT  >  NC_002947/926946‑927239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑