Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 927,711 G→A 7.5% L5971L (CTG→TTG)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947927,7110GA 7.5% 155.7 / 2.3 53L5971L (CTG→TTG) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (26/23);  new base A (2/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATGGAGCTGAGCAAGCCGATGCCGCCGGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGG  >  NC_002947/927576‑927857
                                                                                                                                       |                                                                                                                                                  
tgATGGAGCTGAGCAAGCCGATGCCGCCGGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACtggccg                                                                                                                                       <  8:88856/149‑1 (MQ=255)
  aTGGAGCTGAGCAAGCCGATGCCGCCGGCGGTGACACGTACGTGGTGGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACtggccggt                                                                                                                                     <  5:343810/149‑1 (MQ=255)
    ggAGCTGAGCAAGCCGATGCCGCCGGCGGTGACACGTACGTGGTAGGTACCCGCGCCCTGGTTCTCGAAGGTATACACGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCCACTGTACCAGCACGTCCtggccggtgg                                                                                                                                   >  8:372779/1‑149 (MQ=255)
       gCTGAGCAAGCCGATGCCGCCGGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACtggccggtggccg                                                                                                                                <  5:395513/149‑1 (MQ=255)
            gCAAGCCGATGCCGCCGGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGttg                                                                                                                           <  6:129303/149‑1 (MQ=255)
                gCCGATGCCGCCGGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGttgagct                                                                                                                       <  7:174592/149‑1 (MQ=255)
                      gccgccGGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACtggccggtggccgg                                                                                                                               >  5:303537/1‑135 (MQ=255)
                      gccgccGGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGCCACCGACTGTACCAGCACGTACtggccggtggccgg                                                                                                                               <  6:303537/135‑1 (MQ=255)
                        cgccgGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGCGCTTGcgcagc                                                                                                               >  8:374225/1‑149 (MQ=255)
                        cgccgGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGagcagc                                                                                                               >  7:185946/1‑149 (MQ=255)
                              cggTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGAtg                                                                                                         <  8:185946/149‑1 (MQ=255)
                                 tGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGAtggtg                                                                                                      <  4:251718/149‑1 (MQ=255)
                                  gACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGAt                                                                                                          <  5:358024/144‑1 (MQ=255)
                                  gACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGAt                                                                                                          >  6:358024/1‑144 (MQ=255)
                                   acacGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATTCCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAACACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATggtggt                                                                                                    >  6:27080/1‑149 (MQ=255)
                                   acacGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATggtggt                                                                                                    <  1:43737/149‑1 (MQ=255)
                                   acacGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAACACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATggtggt                                                                                                    >  6:32607/1‑149 (MQ=255)
                                        tacgtGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCggg                                                                                                                              <  4:98420/118‑1 (MQ=255)
                                        tacgtGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCggg                                                                                                                              >  3:98420/1‑118 (MQ=255)
                                             ggtaggtaCCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGttgagcttg                                                                                                                     <  1:237905/122‑1 (MQ=255)
                                             ggtaggtaCCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGttgagcttg                                                                                                                     >  2:237905/1‑122 (MQ=255)
                                                   taCCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTc                                                                                    <  7:24490/149‑1 (MQ=255)
                                                    aCCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTc                                                                                    >  3:125521/1‑148 (MQ=255)
                                                      cGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCaa                                                                                  >  7:320183/1‑148 (MQ=255)
                                                           cccTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGtt                                                                            >  1:266578/1‑149 (MQ=255)
                                                              tGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTtggt                                                                         >  4:151326/1‑149 (MQ=255)
                                                               ggTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTtggtg                                                                        >  8:34760/1‑149 (MQ=255)
                                                                gTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTtggtg                                                                        <  1:87239/148‑1 (MQ=255)
                                                                 ttCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGa                                                                      <  8:205070/149‑1 (MQ=255)
                                                                         ggTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGacca                                                              <  7:374225/149‑1 (MQ=255)
                                                                            aaaCCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCAGAGTTGAGCTTGAGCAGCTCGAAGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGaccacca                                                           >  7:255370/3‑149 (MQ=255)
                                                                                   gcgcCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAACCCGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAg                                                    <  5:32607/149‑1 (MQ=255)
                                                                                   gcgcCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAACACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAg                                                    <  5:27080/149‑1 (MQ=255)
                                                                                       cgccgccCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGaccaccacc                                                         >  4:69593/1‑140 (MQ=255)
                                                                                       cgccgccCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGaccaccacc                                                         <  3:69593/140‑1 (MQ=255)
                                                                                             ccAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTg                                          <  3:151326/149‑1 (MQ=255)
                                                                                                gTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGaccacca                                                           <  1:170614/129‑1 (MQ=255)
                                                                                                gTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGaccacca                                                           >  2:170614/1‑129 (MQ=255)
                                                                                                     cTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTgg                                   <  2:266578/148‑1 (MQ=255)
                                                                                                      tGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTggcg                                 >  5:201063/1‑149 (MQ=255)
                                                                                                       gACCAGCGAACCACCTGGCACCGACTGAACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTggcgg                                >  1:16280/1‑149 (MQ=255)
                                                                                                          cAGCGAACCACCTGGCATCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCaa                             >  2:251979/1‑149 (MQ=255)
                                                                                                          cAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCaa                             >  2:78081/1‑149 (MQ=255)
                                                                                                            gCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAAcc                           >  4:124000/1‑149 (MQ=255)
                                                                                                               aaCCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCgtg                        >  7:249488/1‑149 (MQ=255)
                                                                                                                        gCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAg                                                    >  6:255963/1‑112 (MQ=255)
                                                                                                                        gCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGgtgt                 <  8:249488/147‑1 (MQ=255)
                                                                                                                        gCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAg               <  6:201063/149‑1 (MQ=255)
                                                                                                                           ccGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTatcag            >  5:82734/1‑145 (MQ=255)
                                                                                                                                   aCCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCg    >  5:182913/1‑149 (MQ=255)
                                                                                                                                    ccAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCg    <  2:229470/148‑1 (MQ=255)
                                                                                                                                     cAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCggg  >  6:122265/1‑149 (MQ=255)
                                                                                                                                     cAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCggg  >  2:183389/1‑149 (MQ=255)
                                                                                                                                       gCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGt             <  5:361473/136‑1 (MQ=255)
                                                                                                                                       gCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGt             >  6:361473/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                                  
TGATGGAGCTGAGCAAGCCGATGCCGCCGGCGGTGACACGTACGTGGTAGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTGAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGG  >  NC_002947/927576‑927857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑