| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 85,077 | T→G | 10.2% | intergenic (+55/+75) | aroE → / ← PP_0075 | shikimate 5‑dehydrogenase/choline sulfate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 85,077 | 0 | T | G | 10.2% | 147.5 / 4.6 | 55 | intergenic (+55/+75) | aroE/PP_0075 | shikimate 5‑dehydrogenase/choline sulfate transporter |
| Reads supporting (aligned to +/- strand): ref base T (28/20); new base G (2/4); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGC > NC_002947/84929‑85221 | gcaggcagCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGt < 3:366492/149‑1 (MQ=255) caggcagCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTa > 5:208703/1‑149 (MQ=255) gcagCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAaagta‑agt > 6:172135/1‑149 (MQ=255) gcagCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑cgt > 5:343209/1‑149 (MQ=255) tGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTGCCCCAAAGTA‑CGTCGTAc > 3:196640/1‑149 (MQ=255) gAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACg > 6:280946/1‑149 (MQ=255) aaGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑cgtc > 8:96858/1‑143 (MQ=255) aaGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑cgtc < 7:96858/143‑1 (MQ=255) aaGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTAc < 4:97136/147‑1 (MQ=255) aaGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGc > 4:96317/1‑149 (MQ=255) ccTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGa > 7:225524/1‑149 (MQ=255) ttcttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATc > 8:292653/1‑149 (MQ=255) cttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATc < 3:96317/147‑1 (MQ=255) cttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑AGTCGTACGCCGATc < 5:172135/147‑1 (MQ=255) aTCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCCCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtg < 7:224356/148‑1 (MQ=255) gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTgg < 6:245601/146‑1 (MQ=255) gtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTTGCAG‑CCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACgg < 1:310310/149‑1 (MQ=18) gtggcgtgAGGCCGGATACGGCGCCGGTATTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGCCGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCg > 3:98086/1‑149 (MQ=255) gtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTGCCACAAAGTA‑CGTCGTTAGCCGCTGCTCGGTGGGAGGGGccgt > 3:206950/1‑147 (MQ=38) aGGCCGGATACGGCGCCGGTGTTGGCTTAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCTTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGc < 5:232319/148‑1 (MQ=255) aGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGc < 3:173707/148‑1 (MQ=255) gCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTc < 1:388434/149‑1 (MQ=18) ccGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTcg > 3:76606/1‑149 (MQ=255) cGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTcgc > 3:253577/1‑149 (MQ=255) gcgggTGTGGGCGGAAGTGCGCCGGCAACGGGCGGGGGGCGGCGCGGGCCGGGGCCGCTTGGCGGCCCTATCGCGCCGCAAGGCCTTTCCCACAATGTA‑CGCCGTCCGCCTATCCTCTGGGGGTGCGGCCTTGCGTCGCGAT‑GGGCTGc < 4:150570/146‑1 (MQ=255) gcCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCACGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGc < 6:208703/149‑1 (MQ=255) ggCTTAAGTGCGCCGGCAACCCGCGCGGGGCTGCGCGGCCGGGGGCCGCTTGGGGCCCCTACCGCCCCCCACGCCCGTCCCCAAAACGAC‑CGGCCTCCGCCGACCCGCTGGGGCCGCGCCCTTGGGCCGCGAT‑GGGCTGCAAAGCAGAc < 4:206950/149‑1 (MQ=255) cTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGCTCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGAcc > 2:90985/1‑148 (MQ=255) tGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCa < 8:225524/149‑1 (MQ=255) cTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCATGCGTCGCGAT‑GGGCTGCAAAGCAGACCAAGAAt > 7:187383/1‑149 (MQ=255) cgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATccc < 6:173617/149‑1 (MQ=255) cgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATcc < 5:163961/148‑1 (MQ=255) cgcCGGCAACTCGCTCGGGCCTGAGCTGGCCGGGGCCGCGTGGCGGCCCTATCGCGACGCAACGCCGGTCCCACACCGGA‑CGGCGGACGCCGCGCAGAGGTGGGAGCGGCCTTTCGTCGCGAT‑GGGATGCAAAGCAAACCCatcatcac > 6:311308/1‑142 (MQ=255) cGGCAACTCGCTCGGGGCGGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGTCGTACGACG‑TACTTTGTggg < 2:241668/101‑1 (MQ=17) gCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCt > 2:56997/1‑149 (MQ=255) gctcgGGGCTGAGCTGGCCGGGGCCGGTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCt < 6:343209/149‑1 (MQ=255) gctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCt < 4:253577/149‑1 (MQ=255) ctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATGCTCAGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATGCCCACCTCATTccg > 2:116212/1‑147 (MQ=255) tGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTGCCACAAAGTA‑CGTCGTTCGCCGATCCTCTGTGGGAGCGGCCTTGCGGCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCCACCGCATTCCTCAAACCGGATCggg > 3:349945/1‑149 (MQ=39) tGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCggg > 6:177215/1‑149 (MQ=255) gggCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAAc < 1:56997/149‑1 (MQ=255) gcggcCCTATCGCGATGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCtt > 8:31337/1‑149 (MQ=255) gcggcCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCtt < 5:280946/149‑1 (MQ=255) gcggcCCTATCGCGACGCAAGGCCCTTCCCACAAAGTA‑AGTCGTACGCCGAACCAATGTGGGAGCGGGCGTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATACCAACCTCATTCCTCAAACCGGATCCGGGACAACTCCAGCCCt > 8:225921/1‑148 (MQ=255) cggcCGTATGGCGCCGCACGGCCGTGCCCCCAAAGGA‑CGGCGTACGCCGAGCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTc < 5:311308/149‑1 (MQ=38) ccTTTCGCGACGCATGGCCGTTCCCACAATTTA‑GTTCTTACGCCGATCCGCTGTGGGTGCGGCCTTGGGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGc < 4:196640/149‑1 (MQ=38) ccTATCGCGACGCAAGGCCGTTCCCACAAACTA‑CGTCGTACGCCCATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCAATACTCAAACCGGAACGGCCACCACTCCACCCCGACCAgg > 2:38958/1‑148 (MQ=38) tCGCGAGGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCtt < 4:76606/147‑1 (MQ=255) tCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAcc > 3:131391/1‑116 (MQ=255) tCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAcc < 4:131391/116‑1 (MQ=255) cagctCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGCACGGCCTTGCGTCGCGATAGGGCTGCAAAGTAGACACAGAATCCCAACCTCATTCCTCAAACCGAATCGGGCACAACGCAACCACTTCCAGCTTGTGCAACTACTCGa > 8:323847/5‑149 (MQ=9) cGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCACGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGAt > 1:101322/1‑148 (MQ=255) cccACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCACCTCCTCGATCACCt > 1:195762/1‑149 (MQ=255) cacaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGc > 3:198024/1‑149 (MQ=255) cacaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGc > 1:144032/1‑149 (MQ=255) cacaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCACCCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGc > 7:131471/1‑149 (MQ=255) aaaGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCACACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGc > 8:240033/1‑149 (MQ=255) | GCAGGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGAT‑GGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGC > NC_002947/84929‑85221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |