| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 953,557 | G→T | 7.4% | E122D (GAG→GAT) | cyoC → | cytochrome bo terminal oxidase subunit III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 953,557 | 0 | G | T | 7.4% | 166.1 / 3.3 | 54 | E122D (GAG→GAT) | cyoC | cytochrome bo terminal oxidase subunit III |
| Reads supporting (aligned to +/- strand): ref base G (22/28); new base T (2/2); total (24/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01 | |||||||||||
TGTTGAAACGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACG > NC_002947/953410‑953702 | tGTTGAAACGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATGTGTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAg > 1:101885/1‑149 (MQ=255) gTTGAAACGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGtt > 6:219438/1‑149 (MQ=255) aaCGCTTTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGTTGTTCGATGGCAAGAAAGCAGGTGTACTTGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAACTCATAGATTTCCATc < 7:357089/149‑1 (MQ=255) aaCGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCAt < 3:233926/148‑1 (MQ=255) cGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCAc < 6:182996/149‑1 (MQ=255) cGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCAc < 7:107527/149‑1 (MQ=255) gCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAACTCATAGATTTCCATcgcg < 2:42224/149‑4 (MQ=255) tgctgctgTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGc > 4:220246/1‑149 (MQ=255) ctgctgTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTACAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGa < 3:377168/149‑1 (MQ=255) ctgTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAg < 6:162453/147‑1 (MQ=255) tCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCtt > 3:110969/1‑149 (MQ=255) gATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAg < 6:296404/139‑1 (MQ=255) gATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAg > 5:296404/1‑139 (MQ=255) gATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGcc > 8:344205/1‑149 (MQ=255) aTCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGccc < 4:110969/149‑1 (MQ=255) aTCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGccc > 1:71146/1‑149 (MQ=255) aCCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCAGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCcgca < 5:230714/149‑1 (MQ=255) tcctCGCCATG‑CTGTAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGTAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGt < 8:262396/146‑1 (MQ=255) gCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGt > 4:59935/1‑149 (MQ=255) ccATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTc < 3:105045/149‑1 (MQ=255) aaGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCgg < 3:207163/148‑1 (MQ=255) cAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTgg > 7:160237/1‑149 (MQ=255) aaGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTggt < 7:344205/149‑1 (MQ=255) gCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTAc < 2:71146/149‑1 (MQ=255) gtgtACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCAc > 5:72651/1‑149 (MQ=255) gtgtACTGGGCTGGTTGGCTGTGACCTTCCTGTACGGTGCACGCTTCATCGCTATGGAAATCGAGGAGTTCCATCACCTGATCAGCTAGGGCTTAGGACCGCAGCGCAGTGGCTTCATGTCGGGCTTCTTCGCCCTGGTAGTTACCCAc > 5:399675/1‑149 (MQ=255) tACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACgg < 3:220246/148‑1 (MQ=255) tACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGt > 6:70150/1‑149 (MQ=255) cTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTc < 1:343098/111‑1 (MQ=255) cTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTc > 2:343098/1‑111 (MQ=255) ggCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTg < 8:272807/147‑1 (MQ=255) ggCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCa > 4:76159/1‑149 (MQ=255) ggCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCa < 5:219438/149‑1 (MQ=255) tGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGt < 3:59935/149‑1 (MQ=255) gggTTGGCGGTGACCTTCCGGTTCGGTGCAGGCTTCATCGCTATGGAAATCTCTGAGTGCCATAACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTTCACGt < 4:385172/148‑1 (MQ=255) cTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCTCTGGTAGGTACCCACGGTCTGCACGTGActgct > 7:29033/1‑149 (MQ=255) gtgACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTgg > 2:291014/1‑149 (MQ=255) cTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGa > 7:61410/1‑149 (MQ=255) cTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGa > 1:138575/1‑149 (MQ=255) ccTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGAt < 3:76159/147‑1 (MQ=255) ttCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGa < 2:111420/148‑1 (MQ=255) ttCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGAt < 8:160237/149‑1 (MQ=255) cGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATg < 6:98600/148‑1 (MQ=255) ggTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATgg < 8:110153/148‑1 (MQ=255) cAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAAt > 4:365559/1‑148 (MQ=255) cAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATc < 2:259089/149‑1 (MQ=255) cATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCTCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCatgat < 8:29033/147‑1 (MQ=255) aTCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTa > 5:357649/1‑149 (MQ=255) aTCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACCGCTGGTCTGATCTGGATGGCAATCATGATGTa > 2:166422/1‑149 (MQ=255) ggtgATGGAACTCATAGATTTACATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTAc > 2:305044/4‑148 (MQ=255) gtgATGGAACTCATAGATTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGTTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTAc > 4:213885/3‑147 (MQ=255) ggAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATc > 7:327976/1‑148 (MQ=255) tGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCAc < 2:101885/148‑1 (MQ=255) tGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACg < 1:291014/149‑1 (MQ=255) tGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACg > 1:271714/1‑149 (MQ=255) gTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGt > 2:287641/1‑93 (MQ=255) gTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGt < 1:287641/93‑1 (MQ=255) | TGTTGAAACGCTGTTCCTGCTGCTGTCCTCGATCACCTTCGGCTTCGCCATG‑CTGAAGATGTTCGATGGCAAGAAAGCAGGTGTACTGGGCTGGTTGGCTGTGACCTTCCTGTTCGGTGCAGGCTTCATCGCGATGGAAATCTATGAGTTCCATCACCTGATCAGCGAGGGCTTCGGCCCGCAGCGCAGTGGCTTCCTGTCGGGCTTCTTCGCCCTGGTAGGTACCCACGGTCTGCACGTGACTGCTGGTCTGATCTGGATGGCAATCATGATGTACCAGATCAACAAGCACG > NC_002947/953410‑953702 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |