Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 955,155 C→T 15.3% intergenic (+109/‑204) cyoE → / → alaC protoheme IX farnesyltransferase/aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947955,1550CT15.3% 91.2 / 6.2 39intergenic (+109/‑204)cyoE/alaCprotoheme IX farnesyltransferase/aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (15/18);  new base T (2/4);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

CAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGA  >  NC_002947/955008‑955292
                                                                                                                                                     |                                                                                                                                         
cAGACCGCTGCGGATGTGCTGATGACCTACGCATGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCt                                                                                                                                          <  8:194261/149‑1 (MQ=255)
cAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCTGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCt                                                                                                                                          <  4:315313/149‑1 (MQ=255)
cAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCt                                                                                                                                          <  2:179567/149‑1 (MQ=255)
cAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCt                                                                                                                                          <  2:251195/149‑1 (MQ=255)
cAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCt                                                                                                                                          <  4:19666/149‑1 (MQ=255)
  gACCGCGGCGGATGTGCTGATGACCTACGCACGCGGACACTGCGGCCTGTTGCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGt                                                                                                                                        <  6:175775/149‑1 (MQ=39)
    ccGCTGCTGATGTGCTGATGACCTACGCAAGCTGAAACTTCTGCCTGTTTCACGAAATCCCCGGACACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAg                                                                                                                                      <  1:376974/149‑1 (MQ=39)
    ccGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAg                                                                                                                                      <  3:219925/149‑1 (MQ=255)
       cTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTT‑CTGTGCTGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AGGAACCTTGTAGGAGc                                                                                                                                  <  7:227531/149‑1 (MQ=25)
          cGGATGTGCTGATGACCTACGCACGCTGACACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCgg                                                                                                                                <  5:346320/149‑1 (MQ=255)
          cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGc                                                                                                                                  <  4:208217/147‑1 (MQ=255)
          cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCgg                                                                                                                                <  5:23069/149‑1 (MQ=255)
                     atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCcgc                                                                                                                      <  8:24215/148‑1 (MQ=255)
                                    tGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTAG‑CGGGTTAAACCGGGACTAC‑AAGGTTGCGGTAGGAGCGGGTTTGCCCGGGGAGAGGGCCGca                                                                                                         >  8:283723/1‑146 (MQ=255)
                                           gctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGt                                                                                                                                        <  3:211245/108‑1 (MQ=255)
                                           gctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGt                                                                                                                                        >  4:211245/1‑108 (MQ=255)
                                                 tGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTGCTAC‑AAGGTTGCTGTAGGAGCGGGTTTTCCCGCGAAGAGGCCAGCACAGaaaaattaaaatt                                                                                         >  7:259401/1‑148 (MQ=255)
                                                               cccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTTAAACCCCTGCTTC‑AAGGTTGCGGTTTGGGCGGGTTTTACCGCGAAGAGGCCCGCCCAGAAAAATAAAAATAACTGGTTTTaaaa                                                                            >  7:36245/1‑148 (MQ=255)
                                                               cccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AGGAACCTTGTAGGAGc                                                                                                                                  <  5:98944/94‑1 (MQ=18)
                                                               cccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AGGAACCTTGTAGGAGc                                                                                                                                  >  6:98944/1‑94 (MQ=18)
                                                               cccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTAAACCcgctcc                                                                                                                   >  4:154755/1‑106 (MQ=255)
                                                               cccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTAAACCcgctcc                                                                                                                   <  3:154755/109‑4 (MQ=255)
                                                                       cTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCATAGCCGGGTAAAACC‑CGCCGACAAAGGTGACGGTGGGGGCGGGGTTAAGC‑CGAAGGGGCCCCCCCACAAAAAAAAAAAAAACAGCTTTTTaaaaaaaaaaat                                                                   >  6:233991/1‑143 (MQ=255)
                                                                             cGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑GGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGaaaa                                                             <  4:256619/149‑1 (MQ=255)
                                                                                            ggggTATTTTTCCTGTGCCGCCCTCTTCG‑CGTATAAACCCGAAAATAA‑AAGTTTCCTTTAGTTGCGTTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACa                                               <  5:233991/148‑1 (MQ=255)
                                                                                                                     ttCG‑CGGGTAAACCCGCTCCGAC‑AAGGTTCCAGTAGGAGCGGGGTTACCCCGGAACAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGAGCTGAAAAGTTCGAATAAAACAGGAATTATTCCTTTACTGGCATCCTg                     >  6:74702/1‑149 (MQ=255)
                                                                                                                        g‑cgGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGttt                  <  8:259401/149‑1 (MQ=255)
                                                                                                                        g‑cgGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGACAGCACAGAAAAATAAAAATTCCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGATATTATACTTAACTGGAATCCTGgtt                  >  2:363793/1‑149 (MQ=37)
                                                                                                                           gggTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGa                                                                >  4:350728/1‑101 (MQ=255)
                                                                                                                           gggTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGa                                                                <  3:350728/101‑1 (MQ=255)
                                                                                                                                 aCCCGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCtaataa                                                   <  7:54410/108‑1 (MQ=21)
                                                                                                                                 aCCCGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCtaataa                                                   >  8:54410/1‑108 (MQ=21)
                                                                                                                                 aCCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTa          >  2:153419/1‑149 (MQ=255)
                                                                                                                                  cccGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAt         >  3:222902/1‑149 (MQ=255)
                                                                                                                                   ccGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc        >  2:35243/1‑149 (MQ=255)
                                                                                                                                   ccGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc        >  8:290760/1‑149 (MQ=255)
                                                                                                                                       tCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttct    >  7:17375/1‑149 (MQ=255)
                                                                                                                                         cTAC‑AAGTTTCTTTTTGGTGGGGTTTAACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttct    <  3:384897/147‑1 (MQ=255)
                                                                                                                                          tAC‑AAGGATCCTGTAGGAGCGCGTTTAACAGCGAAGAGGCCATCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAAAAGGAAATTATCCTTTACATTCATACTGTTTCGGAACTATCTTATGa  >  6:215947/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                         
CAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCG‑CGGGTAAACCCGCTCCTAC‑AAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGA  >  NC_002947/955008‑955292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑