Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 998,237 T→C 7.2% S628G (AGC→GGC)  PP_0861 ← outer membrane ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947998,2370TC 7.2% 154.6 / 2.1 55S628G (AGC→GGC) PP_0861outer membrane ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base C (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

TGGTCCACAGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGT  >  NC_002947/998093‑998371
                                                                                                                                                |                                                                                                                                      
tggtCCACAGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCTAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCg                                                                                                                                    >  8:346656/1‑149 (MQ=255)
tggtCCACAGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCg                                                                                                                                    >  5:76247/1‑149 (MQ=255)
     cacaGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAg                                                                                                                               >  7:290055/1‑149 (MQ=255)
     cacaGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAg                                                                                                                               >  4:101524/1‑149 (MQ=255)
       caGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCt                                                                                                                             <  3:116502/149‑1 (MQ=255)
          gCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGa                                                                                                                          >  7:128300/1‑149 (MQ=255)
          gCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTGTCCGCCAGCGGCAAGCTcac                                                                                                                          <  1:158355/149‑3 (MQ=255)
             ggCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATAc                                                                                                                       >  1:154346/1‑149 (MQ=255)
              gcgcTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCTAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATAcc                                                                                                                      <  7:346656/149‑1 (MQ=255)
               cgcTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATAccc                                                                                                                     <  6:190080/149‑1 (MQ=255)
                gcTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCt                                                                                                                    <  2:362179/149‑1 (MQ=255)
                  tgttgtTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGc                                                                                                                  <  6:76247/149‑1 (MQ=255)
                  tgttgtTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTGTCCGCCAGCGGCAAGCTcacc                                                                                                                         >  1:226562/1‑139 (MQ=255)
                  tgttgtTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTGTCCGCCAGCGGCAAGCTcacc                                                                                                                         <  2:226562/142‑4 (MQ=255)
                   gttgttCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCa                                                                                                                 >  8:85229/1‑149 (MQ=255)
                         cGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCTTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCgg                                                                                                            <  8:290055/148‑1 (MQ=255)
                            cGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGttt                                                                                                        <  8:273713/149‑1 (MQ=255)
                                 tCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCAcgcg                                                                                                             <  6:90716/139‑1 (MQ=255)
                                 tCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCAcgcg                                                                                                             >  5:90716/1‑139 (MQ=255)
                                   ggCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACCCCTGCAACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGAc                                                                                                 >  3:78187/1‑149 (MQ=255)
                                         tGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTtctc                                                                                           >  6:9044/1‑149 (MQ=255)
                                           aTTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCgt                                                                                         <  3:127717/149‑1 (MQ=255)
                                              gCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCgtagg                                                                                      >  4:8162/1‑149 (MQ=255)
                                               ccGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCgtagg                                                                                      >  5:77469/1‑148 (MQ=255)
                                                         ccTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTcc                                                                           <  1:229949/149‑1 (MQ=255)
                                                           ttGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTCTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCAc                                                                         >  2:146502/1‑149 (MQ=255)
                                                              cccAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCt                                                                       <  8:128300/148‑1 (MQ=255)
                                                                cAGCGGGCCGCCGTCGATCTGGCGAGCCGGCATGTAGGTGTAGCAGGCAAACACCTGCCACTTGTCGTTGAGCTTGCCGCTGGCGGACTGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGa                                                                    <  6:190163/149‑1 (MQ=255)
                                                                     ggCCGCCGTCGATCTGGCGAGCATGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATAACCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCgg                                                               >  6:77067/1‑149 (MQ=255)
                                                                           cgTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGttt                                                         <  3:101524/149‑1 (MQ=255)
                                                                                 tCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTtc                                                   >  7:360380/1‑149 (MQ=255)
                                                                                         gCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCg                                           <  4:38433/149‑1 (MQ=255)
                                                                                           cTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCgg                                          >  4:2318/1‑148 (MQ=255)
                                                                                              cATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAAca                                      <  6:144749/149‑1 (MQ=255)
                                                                                                     gtgtAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTg                                             <  6:161865/135‑1 (MQ=255)
                                                                                                     gtgtAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTg                                             >  5:161865/1‑135 (MQ=255)
                                                                                                        tAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTc                                                       >  4:154744/1‑122 (MQ=255)
                                                                                                        tAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTc                                                       <  3:154744/122‑1 (MQ=255)
                                                                                                          gCCGGCAAACACCTGCCTCTTGTGGGTGTGCTTGCCGCTGGCGGACAGCTCGATAGCGTGCACGCGGGTTTGGGCGAGGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTc                           <  3:4813/148‑1 (MQ=255)
                                                                                                             ggCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGc                        >  6:20082/1‑148 (MQ=255)
                                                                                                                aaaCACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGa                      >  5:13348/1‑147 (MQ=255)
                                                                                                                aaaCACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACa                    <  7:85229/149‑1 (MQ=255)
                                                                                                                aaaCACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTGCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACa                    <  6:207214/149‑1 (MQ=255)
                                                                                                                     ccTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGgcgc               >  3:391535/1‑149 (MQ=255)
                                                                                                                         ccACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTgg            <  3:44157/148‑1 (MQ=255)
                                                                                                                          cACTTGTCGGTGAGCTTCCCGCTGGCGGACCGCTCGATACCCTGCACGGGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACGGGCGCTGGTc          <  4:78187/149‑1 (MQ=255)
                                                                                                                           aCTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCg         <  5:9044/149‑1 (MQ=255)
                                                                                                                             ttGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAg       >  2:47793/1‑149 (MQ=255)
                                                                                                                             ttGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAg       >  7:335998/1‑149 (MQ=255)
                                                                                                                               gTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAg       >  1:115631/1‑147 (MQ=255)
                                                                                                                                  ggTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGTAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGt  <  5:77067/149‑1 (MQ=255)
                                                                                                                                  ggTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGt  <  2:115631/149‑1 (MQ=255)
                                                                                                                                  atcgAGCTGTCCGCCAGCGGCAAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGt  >  3:382882/4‑149 (MQ=255)
                                                                                                                                        cTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCtt                                                    <  4:339479/93‑1 (MQ=255)
                                                                                                                                        cTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCtt                                                    >  3:339479/1‑93 (MQ=255)
                                                                                                                                                |                                                                                                                                      
TGGTCCACAGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGT  >  NC_002947/998093‑998371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑