Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 998,243 A→C 7.4% S626A (TCC→GCC)  PP_0861 ← outer membrane ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947998,2430AC 7.4% 157.2 / 3.8 54S626A (TCC→GCC) PP_0861outer membrane ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base A (24/26);  new base C (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CACAGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGTCCCATTTGGTGCCGATTT  >  NC_002947/998098‑998389
                                                                                                                                                 |                                                                                                                                                  
cacaGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAg                                                                                                                                                 >  7:290055/1‑149 (MQ=255)
cacaGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAg                                                                                                                                                 >  4:101524/1‑149 (MQ=255)
  caGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCt                                                                                                                                               <  3:116502/149‑1 (MQ=255)
     gCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGa                                                                                                                                            >  7:128300/1‑149 (MQ=255)
     gCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTGTCCGCCAGCGGCAAGCTcac                                                                                                                                            <  1:158355/149‑3 (MQ=255)
        ggCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATAc                                                                                                                                         >  1:154346/1‑149 (MQ=255)
         gcgcTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCTAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATAcc                                                                                                                                        <  7:346656/149‑1 (MQ=255)
          cgcTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATAccc                                                                                                                                       <  6:190080/149‑1 (MQ=255)
           gcTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCt                                                                                                                                      <  2:362179/149‑1 (MQ=255)
             tgttgtTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGc                                                                                                                                    <  6:76247/149‑1 (MQ=255)
             tgttgtTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTGTCCGCCAGCGGCAAGCTcacc                                                                                                                                           >  1:226562/1‑139 (MQ=255)
             tgttgtTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTGTCCGCCAGCGGCAAGCTcacc                                                                                                                                           <  2:226562/142‑4 (MQ=255)
              gttgttCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCa                                                                                                                                   >  8:85229/1‑149 (MQ=255)
                    cGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCTTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCgg                                                                                                                              <  8:290055/148‑1 (MQ=255)
                       cGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGttt                                                                                                                          <  8:273713/149‑1 (MQ=255)
                            tCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCAcgcg                                                                                                                               >  5:90716/1‑139 (MQ=255)
                            tCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCAcgcg                                                                                                                               <  6:90716/139‑1 (MQ=255)
                              ggCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACCCCTGCAACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGAc                                                                                                                   >  3:78187/1‑149 (MQ=255)
                                    tGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTtctc                                                                                                             >  6:9044/1‑149 (MQ=255)
                                      aTTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCgt                                                                                                           <  3:127717/149‑1 (MQ=255)
                                         gCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCgtagg                                                                                                        >  4:8162/1‑149 (MQ=255)
                                          ccGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCgtagg                                                                                                        >  5:77469/1‑148 (MQ=255)
                                                    ccTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTcc                                                                                             <  1:229949/149‑1 (MQ=255)
                                                      ttGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTCTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCAc                                                                                           >  2:146502/1‑149 (MQ=255)
                                                         cccAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCt                                                                                         <  8:128300/148‑1 (MQ=255)
                                                           cAGCGGGCCGCCGTCGATCTGGCGAGCCGGCATGTAGGTGTAGCAGGCAAACACCTGCCACTTGTCGTTGAGCTTGCCGCTGGCGGACTGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGa                                                                                      <  6:190163/149‑1 (MQ=255)
                                                                ggCCGCCGTCGATCTGGCGAGCATGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATAACCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCgg                                                                                 >  6:77067/1‑149 (MQ=255)
                                                                      cgTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGttt                                                                           <  3:101524/149‑1 (MQ=255)
                                                                            tCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTtc                                                                     >  7:360380/1‑149 (MQ=255)
                                                                                    gCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCg                                                             <  4:38433/149‑1 (MQ=255)
                                                                                      cTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCgg                                                            >  4:2318/1‑148 (MQ=255)
                                                                                         cATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAAca                                                        <  6:144749/149‑1 (MQ=255)
                                                                                                gtgtAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTg                                                               >  5:161865/1‑135 (MQ=255)
                                                                                                gtgtAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTg                                                               <  6:161865/135‑1 (MQ=255)
                                                                                                   tAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTc                                                                         <  3:154744/122‑1 (MQ=255)
                                                                                                   tAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTc                                                                         >  4:154744/1‑122 (MQ=255)
                                                                                                     gCCGGCAAACACCTGCCTCTTGTGGGTGTGCTTGCCGCTGGCGGACAGCTCGATAGCGTGCACGCGGGTTTGGGCGAGGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTc                                             <  3:4813/148‑1 (MQ=255)
                                                                                                        ggCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGc                                          >  6:20082/1‑148 (MQ=255)
                                                                                                           aaaCACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGa                                        >  5:13348/1‑147 (MQ=255)
                                                                                                           aaaCACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACa                                      <  7:85229/149‑1 (MQ=255)
                                                                                                           aaaCACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTGCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACa                                      <  6:207214/149‑1 (MQ=255)
                                                                                                                ccTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGgcgc                                 >  3:391535/1‑149 (MQ=255)
                                                                                                                    ccACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTgg                              <  3:44157/148‑1 (MQ=255)
                                                                                                                     cACTTGTCGGTGAGCTTCCCGCTGGCGGACCGCTCGATACCCTGCACGGGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACGGGCGCTGGTc                            <  4:78187/149‑1 (MQ=255)
                                                                                                                      aCTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCg                           <  5:9044/149‑1 (MQ=255)
                                                                                                                        ttGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAg                         >  2:47793/1‑149 (MQ=255)
                                                                                                                        ttGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAg                         >  7:335998/1‑149 (MQ=255)
                                                                                                                          gTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAg                         >  1:115631/1‑147 (MQ=255)
                                                                                                                             ggTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGTAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGt                    <  5:77067/149‑1 (MQ=255)
                                                                                                                             ggTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGt                    <  2:115631/149‑1 (MQ=255)
                                                                                                                             atcgAGCTGTCCGCCAGCGGCAAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGt                    >  3:382882/4‑149 (MQ=255)
                                                                                                                                   cTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCtt                                                                      <  4:339479/93‑1 (MQ=255)
                                                                                                                                   cTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCtt                                                                      >  3:339479/1‑93 (MQ=255)
                                                                                                                                               ggACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGTCCCATTTGGTGCCGAttt  >  4:329210/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CACAGGCTGGCGCTGTTGTTCGGCGTGTTCGGCAGCTGATTGCCGTCGTTGGCCTTGCCCAGCGGGCCGCCGTCGATCTGGCGAGCCTGCATGTAGGTGTAGCCGGCAAACACCTGCCACTTGTCGGTGAGCTTGCCGCTGGCGGACAGCTCGATACCCTGCACGCGGGTTTCGCCGACGTTCTCGTAGGTAGTGGTGTCCACCTGGACGCGGGCGTTTTCCTTCTCGGTGCGGAACAGCGCAGCGGTCAGCGACAGGCGCTGGTCGAGCAGGTCCCATTTGGTGCCGATTT  >  NC_002947/998098‑998389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑