Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,004,526 A→T 6.9% Q184L (CAG→CTG)  PP_0866 → transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,004,5260AT 6.9% 203.2 / 4.4 72Q184L (CAG→CTG) PP_0866transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base A (33/34);  new base T (2/3);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

CGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCATAT  >  NC_002947/1004379‑1004670
                                                                                                                                                     |                                                                                                                                                
cGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAg                                                                                                                                                 <  7:14435/149‑1 (MQ=255)
 ggATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAgg                                                                                                                                                <  3:369528/149‑1 (MQ=255)
       cTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAgtg                                                                                                                                          <  8:302468/149‑1 (MQ=255)
       cTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAgtg                                                                                                                                          <  7:254854/149‑1 (MQ=255)
        tCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAgtgt                                                                                                                                         >  4:383119/1‑149 (MQ=255)
        tCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAgtgt                                                                                                                                         >  5:281691/1‑149 (MQ=255)
        tCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAgtgt                                                                                                                                         >  6:294134/1‑149 (MQ=255)
         cGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTC‑ACACTGCCCTGGGCAgtgtc                                                                                                                                        >  8:40705/1‑147 (MQ=255)
          gCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCTGGGCAgtgt                                                                                                                                         <  4:271573/147‑1 (MQ=255)
           cATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCTGGGCAgtgtcga                                                                                                                                      <  5:155337/149‑4 (MQ=255)
           cATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTC‑ACACTGCCCTGGGCAgtgtcgac                                                                                                                                     <  7:40705/149‑5 (MQ=255)
            aTCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATc                                                                                                                                     <  1:186787/149‑1 (MQ=255)
                 gAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTTATCGTca                                                                                                                                 <  8:38331/148‑1 (MQ=255)
                 gAGCTCAATGGCAACACGCAGGTGCAGGGCGAGTGCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGt                                                                                                                                   <  8:132341/146‑1 (MQ=255)
                     tCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAgg                                                                                                                             >  4:53319/1‑148 (MQ=255)
                     tCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAgg                                                                                                                             >  2:257331/1‑148 (MQ=255)
                      cAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGAG‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAgg                                                                                                                             >  1:107577/1‑148 (MQ=255)
                      cAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑CACTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAgg                                                                                                                             >  1:102440/1‑148 (MQ=255)
                      cAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTa                                                                                                                           >  1:209428/1‑149 (MQ=255)
                       aaTGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGGGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCCGCGTCGCCCAGGACAGTACCCGGCCGTTTGGGGTAGACACTGCCCAGGGCAGTGTGATCGGACCAGGTAc                                                                                                                          >  8:316869/1‑149 (MQ=255)
                          ggCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCg                                                                                                                       >  2:367582/1‑149 (MQ=255)
                              acacGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCttt                                                                                                                   >  8:5868/1‑149 (MQ=255)
                               cacGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTg                                                                                                                  <  8:59108/149‑1 (MQ=255)
                                 cGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGAc                                                                                                                <  3:383119/149‑1 (MQ=255)
                                 cGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGAc                                                                                                                <  5:294134/149‑1 (MQ=255)
                                            gTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTcac                                                                                                                                >  4:101932/1‑122 (MQ=255)
                                            gTCGAGTGCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTg                                                                                                       <  8:337504/147‑1 (MQ=255)
                                               gAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCt                                                                                                        <  6:56500/143‑1 (MQ=255)
                                               gAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCt                                                                                                        >  5:56500/1‑143 (MQ=255)
                                               gAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGAcc                                                                                                   >  3:28332/1‑148 (MQ=255)
                                                aGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACcca                                                                                                 <  6:302168/149‑1 (MQ=255)
                                                  ttCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTcaca                                                                                                                               >  8:73475/1‑117 (MQ=255)
                                                  ttCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTcaca                                                                                                                               <  7:73475/117‑1 (MQ=255)
                                                     aGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACccagcca                                                                                             <  6:264836/148‑1 (MQ=255)
                                                            gCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGt                                                                                                                            <  8:357804/110‑1 (MQ=255)
                                                            gCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGt                                                                                                                            >  7:357804/1‑110 (MQ=255)
                                                                ccGGCACGTGAG‑C‑CTCAACGCTCGTGAAGTGATGTTCAGCGTCGCCCATGAAAGTAACCGGCCGTTTGTGGTCGACACTCACCAGGGCACTGTGATCGTCACAGGAACCCGCTTAGACCTCCGCCAGGACCCAGCCAGCACCGGtctgc                                                                                 >  8:350280/2‑148 (MQ=255)
                                                                 cGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGc                                                                                >  4:74686/1‑149 (MQ=255)
                                                                      cGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTgg                                                                           <  6:281691/149‑1 (MQ=255)
                                                                             c‑cTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAgg                                                                     >  7:259804/1‑149 (MQ=255)
                                                                             c‑cTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAgg                                                                     >  7:265068/1‑149 (MQ=255)
                                                                               cTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCCCCCGTGTGGCGGTGGAACAggg                                                                    >  6:21332/1‑149 (MQ=255)
                                                                                 gAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGt                                                                  >  7:64205/1‑149 (MQ=255)
                                                                                            aaGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGCTCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTg                                                       >  3:372989/1‑149 (MQ=255)
                                                                                               tGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCCGCCAGCACCCGGGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAg                                                    >  8:61749/1‑149 (MQ=255)
                                                                                                 aTGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAgg                                                   <  7:350280/148‑1 (MQ=255)
                                                                                                    ttCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGc                                                 >  7:42971/1‑147 (MQ=255)
                                                                                                       aGCGTCGACCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAgg                                             <  8:354328/148‑1 (MQ=255)
                                                                                                        gCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCgt                                                                                     >  1:178597/1‑107 (MQ=255)
                                                                                                        gCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCgt                                                                                     <  2:178597/107‑1 (MQ=255)
                                                                                                          gTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGa                                         <  2:209428/149‑1 (MQ=255)
                                                                                                               ccATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCaa                                               <  4:128080/138‑1 (MQ=255)
                                                                                                               ccATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCaa                                               >  3:128080/1‑138 (MQ=255)
                                                                                                                cATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGcc                                   >  4:60009/1‑149 (MQ=255)
                                                                                                                cATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGACACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGcc                                   >  8:232075/1‑149 (MQ=255)
                                                                                                                    aCAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGgc                                <  1:19014/148‑1 (MQ=255)
                                                                                                                        tAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAAc                           <  3:53319/149‑1 (MQ=255)
                                                                                                                         aaCCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACt                          >  4:366711/1‑149 (MQ=255)
                                                                                                                          aCCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGGGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACt                          <  5:21332/148‑1 (MQ=255)
                                                                                                                           ccGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGa                        >  6:261211/1‑149 (MQ=255)
                                                                                                                                  tttGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCgg                 >  8:31799/1‑149 (MQ=255)
                                                                                                                                    tgtgGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCggg                <  5:261211/148‑1 (MQ=255)
                                                                                                                                        gTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAggg           >  2:257412/1‑149 (MQ=255)
                                                                                                                                          tcacacTGCCCTGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGt         >  8:64197/3‑149 (MQ=255)
                                                                                                                                          cGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGt         <  1:257412/149‑1 (MQ=255)
                                                                                                                                                tGCCCAGGGCAGTGTGATCGTCACAGGTACCAGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCata   <  2:102440/149‑1 (MQ=255)
                                                                                                                                                  cccAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGNGTCGCata   <  8:265068/147‑1 (MQ=255)
                                                                                                                                                  cccAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCatat  <  3:74686/148‑1 (MQ=255)
                                                                                                                                                  cccAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCatat  <  3:60009/148‑1 (MQ=255)
                                                                                                                                                  cccAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCata   <  8:259804/147‑1 (MQ=255)
                                                                                                                                                  cccAGGGCAGTGTGATCGTCACAGGTACCCGCTTGGACGTCCGCCTGGACCCAGCCCGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCatat  <  4:372989/148‑1 (MQ=255)
                                                                                                                                                  cccAGGGCAGTGTGATCGGCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCata   <  2:107577/147‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCG‑C‑CTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGTAACCGGCCGTTTGTGGTCGACACTGCCCAGGGCAGTGTGATCGTCACAGGTACCCGCTTTGACGTCCGCCTGGACCCAGCCAGCACCCGTGTGGCGGTGGAACAGGGGTCGGTGCGGGTGCAGGGCAAGGGCGACTCGCCGGCGCAACTGACGGCCGGGCAGGGGTCGCATAT  >  NC_002947/1004379‑1004670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑