Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,017,452	G→T	9.6%	S137* (TCA→TAA)	PP_5461 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,017,452	0	G	T	9.6%	202.8 / 9.4	70	S137* (TCA→TAA)	PP_5461	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/26);  new base T (4/3);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGATCATTCTGATCTGGTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGACTATCAAATCTATCTTGCAAAAGT  >  NC_002947/1017306‑1017596                                                                                                                                                     |                                                                                                                                                 gTGATCATTCTGATCTGGTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGTAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCtgat                                                                                                                                                <  3:360276/149‑1 (MQ=255)   gATCATTCTGATCTGGTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCtgatg                                                                                                                                               >  8:144714/1‑148 (MQ=255)    aTCATTCTGATCTGGTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCtgatga                                                                                                                                              <  4:174624/148‑1 (MQ=255)        ttCTGATCTGGTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAacac                                                                                                                                          >  4:300432/1‑148 (MQ=255)        ttCTGATCTGGTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAacac                                                                                                                                          >  6:287621/1‑148 (MQ=255)           tGATCTGGTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCAA‑GTTTGGTGTTCTGATGAACACCa                                                                                                                                        >  3:37190/1‑147 (MQ=255)               cTGGTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACATCTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATATTTCCT‑GTTTGGTGTTCTGATGAACACCAAAGGc                                                                                                                                   <  4:125819/148‑1 (MQ=255)                  gTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTc                                                                                                                                <  3:28722/148‑1 (MQ=255)                   ttCTTTATTCATTGAGATTTTGGTGGACGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGt                                                                                                                              <  4:156060/149‑1 (MQ=255)                   ttCTTTATTCATTGAGATTTTGGGGGACGCTTTGCGGCCCGTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGTTGTCGTGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGt                                                                                                                              <  8:183452/149‑1 (MQ=255)                       ttATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGAc                                                                                                                           <  4:37190/148‑1 (MQ=255)                             aTTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTttct                                                                                                                    >  7:305424/1‑149 (MQ=255)                              ttGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGACCACCAAAGGCCTCGTGACATTttctt                                                                                                                   >  8:222016/1‑149 (MQ=255)                                 agaTTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCg                                                                                                                 <  8:257061/148‑1 (MQ=255)                                   aTTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGta                                                                                                              >  6:48568/1‑149 (MQ=255)                                    ttttGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGta                                                                                                              >  3:241859/1‑148 (MQ=255)                                       aaagggCCGCAAAGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGtatat                                                                                                           >  8:255004/4‑148 (MQ=255)                                             ccGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGt                                                                                                    <  7:47019/149‑1 (MQ=255)                                              cGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGta                                                                                                              <  6:255641/138‑1 (MQ=255)                                              cGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGta                                                                                                              >  5:255641/1‑138 (MQ=255)                                              cGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTa                                                                                                   <  7:304723/149‑1 (MQ=255)                                               gCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGGAGCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTGTCATGAAAACCAAAGGCCTCGTGACATTTAATTCGGTATATAAATTGTa                                                                                                   >  1:106704/1‑149 (MQ=255)                                                 tttGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGAt                                                                                                >  4:116187/1‑149 (MQ=255)                                                   tGCGGCCCTTTTGCTACAACTCTTCGCTACAATAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGCACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGAttt                                                                                              >  4:116461/1‑149 (MQ=255)                                                      ggCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTg                                                                                           >  6:380601/1‑149 (MQ=255)                                                      ggCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTg                                                                                           >  6:260073/1‑149 (MQ=255)                                                       gCCCTTTTTCTACAACTCTTCGCTACATAAGGCGTGTTCAAGTCAGG‑GCCCGGATGTCTGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTgg                                                                                          <  6:72462/149‑1 (MQ=255)                                                       gCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTgg                                                                                          <  5:329513/149‑1 (MQ=255)                                                         ccTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTgggg                                                                                        <  7:255004/149‑1 (MQ=255)                                                          cTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCtgatg                                                                                                                                               >  3:115806/1‑93 (MQ=255)                                                          cTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCtgatg                                                                                                                                               <  4:115806/93‑1 (MQ=255)                                                             ttGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGa                                                                                      <  3:116187/147‑1 (MQ=255)                                                              tGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAgt                                                                                    <  7:362115/148‑1 (MQ=255)                                                                cTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTa                                                                                                   >  6:204115/1‑131 (MQ=255)                                                                cTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTa                                                                                                   >  6:149604/1‑131 (MQ=255)                                                                cTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTa                                                                                                   <  5:204115/131‑1 (MQ=255)                                                                cTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTa                                                                                                   <  5:149604/131‑1 (MQ=255)                                                                    aaCTCTTCTCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGCAATTTTCTTCGGTATATAAATTGTAGATTTTTGTGGGAGTGTGACaa                                                                             >  4:244004/1‑149 (MQ=255)                                                                         ttCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCg                                                                        >  6:362549/1‑149 (MQ=255)                                                                         ttCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTACTGATGAACACCAAAGGCCTCATGACATTTACTTCGGTATATAAATTGTAGATTATAGGGTGAGTTTGCCAATGCCg                                                                        >  7:187531/1‑149 (MQ=255)                                                                            gCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTttctt                                                                                                                   <  6:393917/103‑1 (MQ=255)                                                                            gCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTttctt                                                                                                                   >  5:393917/1‑103 (MQ=255)                                                                                  aaaGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCg                                                               >  4:221200/1‑149 (MQ=255)                                                                                      gCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGc                                                           <  5:260073/149‑1 (MQ=255)                                                                                      gCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGc                                                           <  3:166957/149‑1 (MQ=255)                                                                                            gCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCt                                                     <  8:305424/149‑1 (MQ=255)                                                                                            gCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGACAATGGCGTATTTTTCGCTGCggtact                                                     >  5:61806/1‑145 (MQ=255)                                                                                             cAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTg                                                    >  8:131817/1‑149 (MQ=255)                                                                                             cAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTg                                                    >  3:69780/1‑149 (MQ=255)                                                                                               aGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTg                                                    >  8:18912/1‑147 (MQ=255)                                                                                               aGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTa                                                  <  3:300432/149‑1 (MQ=255)                                                                                                  cAGG‑GACCGGATGGCGTGCTTGAGCTCAATAAATCCA‑GTTTGGTGTTCTTATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGat                                               <  3:244004/149‑1 (MQ=255)                                                                                                   aGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCAGGTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGa                                                >  1:69352/1‑148 (MQ=255)                                                                                                    gg‑gCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTATTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGatat                                             <  4:69780/149‑1 (MQ=255)                                                                                                    gg‑gCCCGGATGTCGGACTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGatat                                             >  3:341220/1‑149 (MQ=255)                                                                                                       gCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGa                                           <  5:48568/149‑1 (MQ=255)                                                                                                                tCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATa                                  <  2:106704/149‑1 (MQ=255)                                                                                                                   ggCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGt                                >  1:353769/1‑148 (MQ=255)                                                                                                                      ttGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATg                            <  7:18912/149‑1 (MQ=255)                                                                                                                        gagaTCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATg                            <  7:144714/147‑1 (MQ=255)                                                                                                                        gagaTCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGa                          >  2:391782/1‑149 (MQ=255)                                                                                                                         agaTCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGAc                         >  7:268244/1‑149 (MQ=255)                                                                                                                          gaTCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCATGTAGATATGAGTGCTCATAGTAATGGAc                         >  3:286340/1‑148 (MQ=255)                                                                                                                           aTCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGTCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGTGGGAGTGTGCCAATGGCGTATTTTTAGCTGCGGTCCTGTAGATATGAGTGCTCATAGAAATGGACTa                       >  3:6027/1‑149 (MQ=255)                                                                                                                           aTCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGACAATGGCGTATTTTTAGCTGCGGGCCTGTAGATATTAGTGCTCATAGTAATGGACTa                       >  5:370620/1‑149 (MQ=255)                                                                                                                              aaTAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGCCATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGATCATAGTAATGGACTATc                     >  3:254583/1‑148 (MQ=255)                                                                                                                                   aTCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGACTATCAAatc                >  1:285016/1‑148 (MQ=255)                                                                                                                                         ctttGGTGTTCATCAGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATa                                  >  7:328469/2‑125 (MQ=255)                                                                                                                                         ctttGGTGTTCATCAGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATa                                  <  8:328469/124‑1 (MQ=255)                                                                                                                                          tttGGTGTTCTTATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGACTATCAAatctatc            <  3:322756/146‑1 (MQ=255)                                                                                                                                          tttGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGACTATCAAatctatc            >  4:322756/1‑146 (MQ=255)                                                                                                                                          tttGGTGTTCATCAGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGACTATCAAATCTATCtt          >  7:364888/1‑148 (MQ=255)                                                                                                                                          tttGGTGTTCATCAGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGACTATCAAATCTATCTTg         >  8:243934/1‑149 (MQ=255)                                                                                                                                          tttGGTGTTCATCAGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGACAATGGAGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGACTATCAAATCTATCtt          >  3:366088/1‑148 (MQ=255)                                                                                                                                                 ttCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGATCATAGTAATGGACTATCAAATCTATCTTGCAAAAGt  >  8:184044/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GTGATCATTCTGATCTGGTTCTTTATGCATTGAGATTTTGGGGGCCGCTTTGCGGCCCTTTTGCTACAACTCTTCGCTACAAAAGGCGTGTGCAAGGCAGG‑GCCCGGATGTCGGGCTTGAGATCAATAAATCCA‑GTTTGGTGTTCTGATGAACACCAAAGGCCTCGTGACATTTTCTTCGGTATATAAATTGTAGATTTTTGGGGGAGTGTGCCAATGGCGTATTTTTCGCTGCGGGCCTGTAGATATGAGTGCTCATAGTAATGGACTATCAAATCTATCTTGCAAAAGT  >  NC_002947/1017306‑1017596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑