Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,054,464 A→T 6.1% intergenic (‑1/+36) PP_0912 ← / ← PP_0913 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,054,4640AT 6.1% 184.2 / 2.2 65intergenic (‑1/+36)PP_0912/PP_0913hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (31/30);  new base T (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

GATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCA  >  NC_002947/1054320‑1054609
                                                                                                                                                |                                                                                                                                                 
gATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCACACGAAAACGCCCGGCACAAGGc                                                                                                                                               >  4:227528/1‑149 (MQ=255)
gATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGc                                                                                                                                               >  8:78019/1‑149 (MQ=255)
 aTTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGcc                                                                                                                                              <  5:7540/149‑1 (MQ=255)
      gCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCg                                                                                                                                         <  2:225973/149‑1 (MQ=255)
           cGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGttt                                                                                                                                      <  6:371839/147‑1 (MQ=255)
             ccccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGc                                                                                                                                    >  6:185621/1‑147 (MQ=255)
             ccccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGc                                                                                                                                    <  5:185621/147‑1 (MQ=255)
               ccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCCTTGTGCCGGGCGTTTTCgttt                                                                                                                                <  1:345140/149‑4 (MQ=255)
                cAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCCTTGTGCCGGGCGTTTTCgttt                                                                                                                                <  8:288546/148‑4 (MQ=255)
                cAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCCTTGTGCCGGGCGTTTTCgttt                                                                                                                                >  7:288546/1‑145 (MQ=255)
                     aTCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAgcgc                                                                                                                          <  7:141715/149‑1 (MQ=255)
                         aGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAca                                                                                                                      <  8:35184/149‑1 (MQ=255)
                                gcagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACATGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGAt                                                                                                               >  2:274596/1‑149 (MQ=255)
                                 cagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAgg                                                                                                                 <  8:337592/146‑1 (MQ=255)
                                       tgtgCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAAc                                                                                                         >  4:256167/1‑148 (MQ=255)
                                             tcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGaa                                                                                                                              <  7:124377/121‑1 (MQ=255)
                                             tcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGaa                                                                                                                              >  8:124377/1‑121 (MQ=255)
                                                  ttAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCa                                                                                             <  3:145174/149‑1 (MQ=255)
                                                   tAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGaa                                                                                                          <  5:10078/135‑1 (MQ=255)
                                                   tAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAg                                                                                            >  5:222536/1‑149 (MQ=255)
                                                   tAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTGTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGGGGTACAGCTGGGTCAGaa                                                                                                          >  6:10078/1‑135 (MQ=255)
                                                    aGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAgt                                                                                           >  5:254935/1‑149 (MQ=255)
                                                      gTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAgtgt                                                                                         >  8:313401/1‑149 (MQ=255)
                                                       tttGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTc                                                                                        >  4:43188/1‑149 (MQ=255)
                                                       tttGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTc                                                                                        >  5:241380/1‑149 (MQ=255)
                                                           atctatggCCTACCGGATGAGCTTCTGCGTGCACGCCAGGCTGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCgg                                                                                     <  6:254935/142‑1 (MQ=255)
                                                           aTCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCggg                                                                                    >  6:384279/1‑149 (MQ=255)
                                                            tCGAAGGTCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGt                                                                                   >  8:174386/1‑149 (MQ=255)
                                                             cGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGACAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGtt                                                                                  >  5:121784/1‑149 (MQ=255)
                                                              gAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTc                                                                                 <  6:222536/149‑1 (MQ=255)
                                                              gAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGCAAGCGTTATACCAGGCAGCAAAAGAAAACGCACGGCACAAGGCCGGGCGTTTGCGCTGAAgcgcaata                                                                                                                      >  1:89911/1‑108 (MQ=255)
                                                                aGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAg                                                                               >  8:318666/1‑149 (MQ=255)
                                                                aGGCCTACAGGATGAGCAGCGGCGTGACCGCCAGCCAGCGGTAAGCGTTTAACCCGGCAGCAAACCAAAACGCCCGGCACATGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAg                                                                               <  4:203266/149‑1 (MQ=255)
                                                                  gCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGt                                                                             <  3:43188/149‑1 (MQ=255)
                                                                   ccTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGtt                                                                            <  6:241380/149‑1 (MQ=255)
                                                                    cTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTg                                                                           >  5:346839/1‑149 (MQ=255)
                                                                    cTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTg                                                                           >  8:353690/1‑149 (MQ=255)
                                                                         ggATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAAt                                                                      >  6:28087/1‑149 (MQ=255)
                                                                          gATGAGCAGCTGCGTGCACGCCTGGCAGCGGGAAGGTTTTTACCCGGCAGCAAACCAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAg                                                                               <  5:252597/139‑1 (MQ=255)
                                                                           aTGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGc                                                                    >  1:235069/1‑149 (MQ=255)
                                                                              aGCATCTGCGTTCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCa                                                                 <  1:274596/149‑1 (MQ=255)
                                                                              aGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCTGGGTTCAGGTTGGCAATGc                                                                    <  5:51753/146‑1 (MQ=255)
                                                                              aGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCTGGGTTCAGGTTGGCAATGc                                                                    >  6:51753/1‑146 (MQ=255)
                                                                                      cGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTg                                                            <  7:318666/146‑1 (MQ=255)
                                                                                         gCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCg                                                        >  1:63713/1‑147 (MQ=255)
                                                                                         gCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCg                                                        >  7:138968/1‑147 (MQ=255)
                                                                                          cACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCa                                                      <  3:256167/148‑1 (MQ=255)
                                                                                             gCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCt                                                  >  5:82109/1‑149 (MQ=255)
                                                                                                aGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGtt                                               <  2:73874/149‑1 (MQ=255)
                                                                                                aGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGtt                                               <  5:28087/149‑1 (MQ=255)
                                                                                                  gCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGc                                                                    <  1:387034/126‑1 (MQ=255)
                                                                                                  gCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCCGCACAAGGCCGGGCGTTTTCGGTGAAGCGCTACAGCAGGATAAGAACTCGTGATACGCAGTGTCCGGGTTAAGGTTGGCAATGc                                                                    >  2:387034/1‑126 (MQ=255)
                                                                                                   cAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcga                                            <  2:63713/149‑1 (MQ=255)
                                                                                                    aGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgat                                           >  2:206681/1‑149 (MQ=255)
                                                                                                    aGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgat                                           <  7:353690/149‑1 (MQ=255)
                                                                                                        ggAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGTCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGAc                                       <  3:190666/149‑1 (MQ=255)
                                                                                                          aaGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGAccc                                     >  6:156043/1‑149 (MQ=255)
                                                                                                               ttttACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCt                                >  7:54916/1‑149 (MQ=255)
                                                                                                                   aCCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCtt                            >  6:311824/1‑149 (MQ=255)
                                                                                                                      cGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGAc                         <  5:156043/149‑1 (MQ=255)
                                                                                                                        gcagcaAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCa                       <  6:346839/149‑1 (MQ=255)
                                                                                                                                   aaaCGCCCGGCCTTGTGCCGGGCGTTTTCGTTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATg            >  2:339489/1‑149 (MQ=255)
                                                                                                                                   aaaCGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATg            <  6:121784/149‑1 (MQ=255)
                                                                                                                                       gCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGt        >  1:352939/1‑149 (MQ=255)
                                                                                                                                            gCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGc   <  3:227528/149‑1 (MQ=255)
                                                                                                                                             cacaAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGca  <  8:138968/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GATTCGGCCTTCGCCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCA  >  NC_002947/1054320‑1054609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑