Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,054,478 G→T 8.9% intergenic (‑15/+22) PP_0912 ← / ← PP_0913 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,054,4780GT 8.9% 204.0 / 6.9 67intergenic (‑15/+22)PP_0912/PP_0913hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base T (3/3);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

CCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCACAACCTGGTTGCC  >  NC_002947/1054333‑1054622
                                                                                                                                                 |                                                                                                                                                
ccccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGc                                                                                                                                                 >  6:185621/1‑147 (MQ=255)
ccccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGc                                                                                                                                                 <  5:185621/147‑1 (MQ=255)
  ccAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCCTTGTGCCGGGCGTTTTCgttt                                                                                                                                             <  1:345140/149‑4 (MQ=255)
   cAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCCTTGTGCCGGGCGTTTTCgttt                                                                                                                                             <  8:288546/148‑4 (MQ=255)
   cAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCCTTGTGCCGGGCGTTTTCgttt                                                                                                                                             >  7:288546/1‑145 (MQ=255)
        aTCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAgcgc                                                                                                                                       <  7:141715/149‑1 (MQ=255)
            aGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTAca                                                                                                                                   <  8:35184/149‑1 (MQ=255)
                   gcagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACATGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGAt                                                                                                                            >  2:274596/1‑149 (MQ=255)
                    cagcaCTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAgg                                                                                                                              <  8:337592/146‑1 (MQ=255)
                          tgtgCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAAc                                                                                                                      >  4:256167/1‑148 (MQ=255)
                                tcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGaa                                                                                                                                           >  8:124377/1‑121 (MQ=255)
                                tcctGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGaa                                                                                                                                           <  7:124377/121‑1 (MQ=255)
                                     ttAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCa                                                                                                          <  3:145174/149‑1 (MQ=255)
                                      tAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGaa                                                                                                                       <  5:10078/135‑1 (MQ=255)
                                      tAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAg                                                                                                         >  5:222536/1‑149 (MQ=255)
                                      tAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTGTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGGGGTACAGCTGGGTCAGaa                                                                                                                       >  6:10078/1‑135 (MQ=255)
                                       aGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAgt                                                                                                        >  5:254935/1‑149 (MQ=255)
                                         gTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAgtgt                                                                                                      >  8:313401/1‑149 (MQ=255)
                                          tttGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTc                                                                                                     >  5:241380/1‑149 (MQ=255)
                                          tttGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTc                                                                                                     >  4:43188/1‑149 (MQ=255)
                                              atctatggCCTACCGGATGAGCTTCTGCGTGCACGCCAGGCTGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCgg                                                                                                  <  6:254935/142‑1 (MQ=255)
                                              aTCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCggg                                                                                                 >  6:384279/1‑149 (MQ=255)
                                               tCGAAGGTCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGt                                                                                                >  8:174386/1‑149 (MQ=255)
                                                cGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGACAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGtt                                                                                               >  5:121784/1‑149 (MQ=255)
                                                 gAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTc                                                                                              <  6:222536/149‑1 (MQ=255)
                                                 gAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGCAAGCGTTATACCAGGCAGCAAAAGAAAACGCACGGCACAAGGCCGGGCGTTTGCGCTGAAgcgcaata                                                                                                                                   >  1:89911/1‑108 (MQ=255)
                                                   aGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAg                                                                                            >  8:318666/1‑149 (MQ=255)
                                                   aGGCCTACAGGATGAGCAGCGGCGTGACCGCCAGCCAGCGGTAAGCGTTTAACCCGGCAGCAAACCAAAACGCCCGGCACATGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAg                                                                                            <  4:203266/149‑1 (MQ=255)
                                                     gCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGt                                                                                          <  3:43188/149‑1 (MQ=255)
                                                      ccTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGtt                                                                                         <  6:241380/149‑1 (MQ=255)
                                                       cTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTg                                                                                        >  8:353690/1‑149 (MQ=255)
                                                       cTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTg                                                                                        >  5:346839/1‑149 (MQ=255)
                                                            ggATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAAt                                                                                   >  6:28087/1‑149 (MQ=255)
                                                             gATGAGCAGCTGCGTGCACGCCTGGCAGCGGGAAGGTTTTTACCCGGCAGCAAACCAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAg                                                                                            <  5:252597/139‑1 (MQ=255)
                                                              aTGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGc                                                                                 >  1:235069/1‑149 (MQ=255)
                                                                 aGCATCTGCGTTCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCa                                                                              <  1:274596/149‑1 (MQ=255)
                                                                 aGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCTGGGTTCAGGTTGGCAATGc                                                                                 <  5:51753/146‑1 (MQ=255)
                                                                 aGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCTGGGTTCAGGTTGGCAATGc                                                                                 >  6:51753/1‑146 (MQ=255)
                                                                         cGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTg                                                                         <  7:318666/146‑1 (MQ=255)
                                                                            gCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCg                                                                     >  7:138968/1‑147 (MQ=255)
                                                                            gCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCg                                                                     >  1:63713/1‑147 (MQ=255)
                                                                             cACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCa                                                                   <  3:256167/148‑1 (MQ=255)
                                                                                gCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCt                                                               >  5:82109/1‑149 (MQ=255)
                                                                                   aGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGtt                                                            <  5:28087/149‑1 (MQ=255)
                                                                                   aGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGtt                                                            <  2:73874/149‑1 (MQ=255)
                                                                                     gCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGc                                                                                 <  1:387034/126‑1 (MQ=255)
                                                                                     gCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCCGCACAAGGCCGGGCGTTTTCGGTGAAGCGCTACAGCAGGATAAGAACTCGTGATACGCAGTGTCCGGGTTAAGGTTGGCAATGc                                                                                 >  2:387034/1‑126 (MQ=255)
                                                                                      cAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcga                                                         <  2:63713/149‑1 (MQ=255)
                                                                                       aGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgat                                                        >  2:206681/1‑149 (MQ=255)
                                                                                       aGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTtcgat                                                        <  7:353690/149‑1 (MQ=255)
                                                                                           ggAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGTCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGAc                                                    <  3:190666/149‑1 (MQ=255)
                                                                                             aaGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGAccc                                                  >  6:156043/1‑149 (MQ=255)
                                                                                                  ttttACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCt                                             >  7:54916/1‑149 (MQ=255)
                                                                                                      aCCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCtt                                         >  6:311824/1‑149 (MQ=255)
                                                                                                         cGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGAc                                      <  5:156043/149‑1 (MQ=255)
                                                                                                           gcagcaAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCa                                    <  6:346839/149‑1 (MQ=255)
                                                                                                                      aaaCGCCCGGCCTTGTGCCGGGCGTTTTCGTTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATg                         >  2:339489/1‑149 (MQ=255)
                                                                                                                      aaaCGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATg                         <  6:121784/149‑1 (MQ=255)
                                                                                                                          gCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGt                     >  1:352939/1‑149 (MQ=255)
                                                                                                                               gCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGc                <  3:227528/149‑1 (MQ=255)
                                                                                                                                cacaAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGca               <  8:138968/149‑1 (MQ=255)
                                                                                                                                     ggCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGGAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCACAAcc          >  3:125276/1‑149 (MQ=255)
                                                                                                                                       ccGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCACAACCTg        <  5:311824/149‑1 (MQ=255)
                                                                                                                                          ggCGTTTTCGTTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCACAACCTGGtt     <  1:339489/149‑1 (MQ=255)
                                                                                                                                          ggCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCACAACCTGGtt     <  7:313401/149‑1 (MQ=255)
                                                                                                                                            cGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCACAACCTGGTTGc   <  2:352939/149‑1 (MQ=255)
                                                                                                                                             gTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCACAACCTGGTTGcc  >  6:253729/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCCCAGACATCAAGAATCTGCAGCACTGTGCCTCCTGTTAGGTTTGATCGAAGGCCTACAGGATGAGCATCTGCGTGCACGCCAGGCAGCGGGAAGCGTTTTACCCGGCAGCAAACGAAAACGCCCGGCACAAGGCCGGGCGTTTGCGGTGAAGCGCTACAGCAGGATCAGAACTCGTGATCCGCAGTGTCCGGGTTCAGGTTGGCAATGCCCAGCTTGCCCGCAGCCTGTTCGATCGACCCGGTCTGCTTGACCAGGGCGGCGATGGCGTCCCGCACAACCTGGTTGCC  >  NC_002947/1054333‑1054622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑