Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,101,799 C→G 6.7% A127G (GCA→GGA) ‡ mlaD → phospholipid ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,101,7990CG 6.7% 284.3 / 8.5 89A127G (GCA→GGA) ‡mlaDphospholipid ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (41/42);  new base G (4/2);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

ACACCGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGT  >  NC_002947/1101652‑1101946
                                                                                                                                                   |                                                                                                                                                   
acacCGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGc                                                                                                                                                     >  4:246311/1‑148 (MQ=255)
    cGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAcca                                                                                                                                                >  7:353385/1‑149 (MQ=255)
    cGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAcca                                                                                                                                                >  2:349514/1‑149 (MQ=255)
    cGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACACCACTGCCTCGATCCTGACAGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAcca                                                                                                                                                >  1:98064/1‑149 (MQ=255)
     ggTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGACGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccat                                                                                                                                               <  1:262422/149‑1 (MQ=255)
       tCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatc                                                                                                                                              >  5:210464/1‑148 (MQ=255)
         gggTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatccat                                                                                                                                           <  7:241850/149‑1 (MQ=255)
           gTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGa                                                                                                                                         <  7:160783/149‑1 (MQ=255)
            tGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCATGGATGGTTGCACCATCCTTGag                                                                                                                                        <  3:209490/149‑2 (MQ=255)
              aCGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGaca                                                                                                                                       >  6:20663/1‑148 (MQ=255)
                   gCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGt                                                                                                                                 >  3:170257/1‑149 (MQ=255)
                     aGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGCGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCg                                                                                                                               >  4:195849/1‑149 (MQ=255)
                      gCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCgg                                                                                                                              <  4:256403/149‑1 (MQ=255)
                          gACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCa                                                                                                                            <  4:141046/147‑1 (MQ=255)
                          gACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCa                                                                                                                            <  3:246311/147‑1 (MQ=255)
                          gACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCAc                                                                                                                           <  7:200649/148‑1 (MQ=255)
                           aCAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTg                                                                                                                         <  6:189373/149‑1 (MQ=255)
                               gtcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTTGCAccatcctt                                                                                                                                           <  5:289245/127‑3 (MQ=255)
                               gtcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTTGCAccatcctt                                                                                                                                           >  6:289245/1‑125 (MQ=255)
                                tcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCt                                                                                                                    >  3:290472/1‑149 (MQ=255)
                                  ggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTg                                                                                                                   >  7:10958/1‑148 (MQ=255)
                                   gtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTgga                                                                                                                 <  4:290472/149‑1 (MQ=255)
                                       aTAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTggagga                                                                                                              >  7:25589/1‑148 (MQ=255)
                                        tAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATc                                                                                                            <  1:13431/149‑1 (MQ=255)
                                           ccTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGa                                                                                                         >  3:137554/1‑149 (MQ=255)
                                            cTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGAt                                                                                                        <  1:349514/149‑1 (MQ=255)
                                             tGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGAtt                                                                                                       >  2:49868/1‑149 (MQ=255)
                                               ccgaccgaCTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGCGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGAtt                                                                                                       <  3:195849/147‑1 (MQ=255)
                                               ccgaccgaCTCCACTGCCTCGATCCTGACCGCCGGTATGCTTGGTGAGAAGTACATCTGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTTGAGGATCTGATTgg                                                                                                     <  2:98064/149‑1 (MQ=255)
                                                     gacTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGGGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGtt                                                                                               >  8:141367/1‑149 (MQ=255)
                                                      acTCCACTGCCTCGCTCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTc                                                                                              <  8:353385/149‑1 (MQ=255)
                                                        tCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCaa                                                                                                  >  5:389163/1‑143 (MQ=255)
                                                        tCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCaa                                                                                                  <  6:389163/143‑1 (MQ=255)
                                                          cACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTg                                                                                           <  5:290038/148‑1 (MQ=255)
                                                          cACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGc                                                                                          >  6:344357/1‑149 (MQ=255)
                                                          cACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGc                                                                                          >  4:129443/1‑149 (MQ=255)
                                                            cTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTc                                                                                        <  6:210464/149‑1 (MQ=255)
                                                                cTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAAct                                                                                    >  2:340101/1‑149 (MQ=255)
                                                                    aTCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGt                                                                                >  5:281109/1‑149 (MQ=255)
                                                                    aTCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGt                                                                                >  1:141079/1‑149 (MQ=255)
                                                                     tCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGtt                                                                               >  8:134391/1‑149 (MQ=255)
                                                                     tCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGtt                                                                               <  3:129443/149‑1 (MQ=255)
                                                                          aCCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCaa                                                                          <  5:344357/149‑1 (MQ=255)
                                                                          aCCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCa                                                                           <  4:170257/148‑1 (MQ=255)
                                                                            cgccgGTTTGCTTGGTGAGAAGTACATCGACATCAGCGTTGGCGGTGGAGACGAAGTGCTCAAGGATTGTGCAACCATACATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTACTGCTAAACTATGTTGGCAAg                                                                         >  1:284175/1‑148 (MQ=255)
                                                                              ccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAggta                                                                      >  6:191671/1‑147 (MQ=255)
                                                                                ggTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCTGTGAAGACGAAGTGCTCAAGGATTGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAAcc                                                                    <  3:317781/149‑1 (MQ=255)
                                                                                    tGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGaa                                                                 <  8:10958/148‑1 (MQ=255)
                                                                                         ggTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCt                                                                                            >  6:191825/1‑116 (MQ=255)
                                                                                         ggTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCt                                                                                            <  5:191825/116‑1 (MQ=255)
                                                                                          gTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTCCTCAAGTATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGATGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAgc                                                           <  4:332857/148‑1 (MQ=255)
                                                                                           tGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGCAgcg                                                          >  3:284727/1‑148 (MQ=255)
                                                                                           tGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAgcgc                                                         <  5:20663/149‑1 (MQ=255)
                                                                                            gagaAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAgcgc                                                         >  5:22970/1‑148 (MQ=255)
                                                                                             agaAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAAc                                                                     >  1:382014/1‑135 (MQ=255)
                                                                                             agaAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAAc                                                                     <  2:382014/135‑1 (MQ=255)
                                                                                                         gCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGATCAACTCTGTTGGCAAGGAACCCAAAGAAGCGCAACCGGCTAAta                                             >  4:304412/1‑146 (MQ=255)
                                                                                                          cATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAg                                          <  2:141079/149‑1 (MQ=255)
                                                                                                           aTCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAg                                          >  6:258333/1‑148 (MQ=255)
                                                                                                              aGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAg                                       >  4:57464/1‑148 (MQ=255)
                                                                                                                cGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTAc                                    <  1:340101/149‑1 (MQ=255)
                                                                                                                  ttGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGa                                        <  5:286729/143‑1 (MQ=255)
                                                                                                                  ttGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGa                                        >  6:286729/1‑143 (MQ=255)
                                                                                                                  ttGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTAcc                                   >  8:136893/1‑148 (MQ=255)
                                                                                                                     gCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGTAAGGAAc                                                                     >  2:252185/1‑111 (MQ=255)
                                                                                                                     gCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGTAAGGAAc                                                                     <  1:252185/111‑1 (MQ=255)
                                                                                                                       ggTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGAt                             >  5:175491/1‑149 (MQ=255)
                                                                                                                         tGAAGACGATGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCAGTGGTGCTGGTGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGAttt                           <  3:304412/149‑1 (MQ=255)
                                                                                                                         tGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGAttt                           <  6:281109/149‑1 (MQ=255)
                                                                                                                          gAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGAttt                           <  5:118313/148‑1 (MQ=255)
                                                                                                                           aaGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTc                          <  6:22970/148‑1 (MQ=255)
                                                                                                                           aaGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTc                          <  7:134391/148‑1 (MQ=255)
                                                                                                                             gACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGAt                       >  2:62707/1‑149 (MQ=255)
                                                                                                                                gAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCaa                                                       >  2:39713/1‑114 (MQ=255)
                                                                                                                                gAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCaa                                                       <  1:39713/114‑1 (MQ=255)
                                                                                                                                gAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCt                    <  7:141367/149‑1 (MQ=255)
                                                                                                                                gAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCt                    >  7:16183/1‑149 (MQ=255)
                                                                                                                                       tctaGGATTGTTCAACCATCGATGACACACAGTGGGCAGTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTaa                                           <  5:32916/116‑1 (MQ=255)
                                                                                                                                       tCATGGATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGACCCGAAAGACGCGCAACCGCCTAATTAAGGAGTACCCATCATTTCGATCATCCGAc               >  7:193136/1‑147 (MQ=255)
                                                                                                                                       tCATGGATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGGATCCGGCTATTTAAGTAGTACCCTTGATTTGGATCCTGCGACGt             >  7:198698/1‑149 (MQ=255)
                                                                                                                                         aaGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTTGCAAGGAACCGAAAGAAGCGCAACCGGCTAATAAAGGAGTACCCATGATTTCGATCCTGCGACGt             <  4:137554/147‑1 (MQ=255)
                                                                                                                                         aaGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTg            <  1:49868/148‑1 (MQ=255)
                                                                                                                                          tggATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTgga          >  1:286199/2‑148 (MQ=255)
                                                                                                                                            gATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCct        >  6:187351/1‑149 (MQ=255)
                                                                                                                                              tGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCctgc      <  7:136893/149‑1 (MQ=255)
                                                                                                                                                gTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCctgctg    >  3:287114/1‑149 (MQ=255)
                                                                                                                                                 tGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTaa                                           <  7:33539/109‑1 (MQ=255)
                                                                                                                                                 tGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTaa                                           >  8:33539/1‑109 (MQ=255)
                                                                                                                                                  gCAACCATCCATGACACACAGTCGGCAGTGGTGCTGGAGCATCTGATTGGCAAGTACCTGCTCAACTCTGTTGCCAAGGAACCGAAACAACCACAACCGGCTATTTAAGGAGTACCCATGATATCGATCCTGCGACGTGGCCTCCAGGt  >  2:110236/1‑149 (MQ=255)
                                                                                                                                                  gCAACCATCCATGACACACAGTCGGCACTGGTGATGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGt  >  7:291519/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACACCGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGT  >  NC_002947/1101652‑1101946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑