Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,145,448	A→G	10.4%	intergenic (‑115/+26)	hemO ← / ← PP_1006	heme oxygenase/heme receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,145,448	0	A	G	10.4%	175.8 / 10.0	67	intergenic (‑115/+26)	hemO/PP_1006	heme oxygenase/heme receptor
Reads supporting (aligned to +/- strand):  ref base A (30/30);  new base G (5/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

CGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCACTG  >  NC_002947/1145302‑1145596                                                                                                                                                   |                                                                                                                                                     cgcTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCaaa                                                                                                                                                    <  7:360537/149‑1 (MQ=255)   cTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAaga                                                                                                                                                  <  2:108548/149‑1 (MQ=255)           gcAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTa                                                                                                                                          >  5:119725/1‑149 (MQ=255)           gcAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCCTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAATAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTa                                                                                                                                          <  8:151234/149‑1 (MQ=255)               ggCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGATGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGa                                                                                                                                      <  3:85242/149‑1 (MQ=255)                gCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCATTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGAc                                                                                                                                     <  1:258542/149‑1 (MQ=255)                           ggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGCCGTAACGCCt                                                                                                                            >  8:353940/1‑147 (MQ=255)                           ggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCa                                                                                                                          <  6:119725/149‑1 (MQ=255)                                  ggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATcc                                                                                                                   <  5:34270/149‑1 (MQ=255)                                  ggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATcc                                                                                                                   <  2:109142/149‑1 (MQ=255)                                  ggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATc                                                                                                                    <  7:353940/148‑1 (MQ=255)                                        tCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAAcc                                                                                                             <  4:250612/149‑1 (MQ=255)                                          cTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATcc                                                                                                                   >  8:193041/1‑141 (MQ=255)                                          cTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATcc                                                                                                                   <  7:193041/141‑1 (MQ=255)                                          cTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACca                                                                                                            >  6:5150/1‑148 (MQ=255)                                          cTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCAGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACca                                                                                                            >  2:208236/1‑148 (MQ=255)                                             ggAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACcaca                                                                                                          <  6:139763/147‑1 (MQ=255)                                             ggAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACAtt                                                                                                        >  6:259450/1‑149 (MQ=255)                                              gAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACAtt                                                                                                        >  2:136306/1‑148 (MQ=255)                                                aaGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGa                                                                                                      >  4:100027/1‑148 (MQ=255)                                                 agagGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTCCCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATa                                                                                                    <  4:300724/149‑1 (MQ=255)                                                 agagGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCATCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATa                                                                                                    <  5:276710/149‑1 (MQ=255)                                                 agagCGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCCGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATa                                                                                                    <  5:362669/149‑1 (MQ=255)                                                     ggCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCg                                                                                                 >  8:267419/1‑148 (MQ=255)                                                        gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGt                                                                                             >  4:224285/1‑149 (MQ=255)                                                                 cAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAaga                                                                                                                                                  <  2:236329/87‑1 (MQ=255)                                                                 cAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAaga                                                                                                                                                  >  1:236329/1‑87 (MQ=255)                                                                  aaGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCaaa                                                                                                               >  6:179833/1‑121 (MQ=255)                                                                  aaGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCaaa                                                                                                               <  5:179833/121‑1 (MQ=255)                                                                         aaCAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCg                                                                                    <  7:369268/141‑1 (MQ=255)                                                                         aaCAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGACGGTACAGACGTAACGCCTCAGCTATCCCAAAACACATTGATACCGAACTTGAGCCCCg                                                                                    >  8:369268/1‑141 (MQ=255)                                                                          aCAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGt                                                                             >  7:89384/1‑147 (MQ=255)                                                                                    cGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                                                 <  7:267419/149‑1 (MQ=255)                                                                                     gagaCAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATg                                                                >  2:264655/1‑149 (MQ=255)                                                                                      agaCAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCAGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACAACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGt                                                               >  3:27769/1‑149 (MQ=255)                                                                                       gaCAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGtt                                                              >  3:26150/1‑149 (MQ=255)                                                                                         cAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtgg                                                            <  3:224285/149‑1 (MQ=255)                                                                                                     ccGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca                                                >  5:135360/1‑149 (MQ=255)                                                                                                           tttGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgccg                                          >  5:260898/1‑149 (MQ=255)                                                                                                              gCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGGCGTAACGGCTCAGATATCCCAAACCCCATTGATACCGAAGTTGGGCCCCGGCATGGTCGGGCGGTCGCTGTTGGCTGGCTGGGGCACAGCCGCCt                                       >  5:63416/1‑149 (MQ=255)                                                                                                                gTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCa                                     >  1:353272/1‑149 (MQ=255)                                                                                                                gTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCa                                     <  7:33126/149‑1 (MQ=255)                                                                                                                          ccGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcgt                           >  2:108577/1‑149 (MQ=255)                                                                                                                           cGGCCTCTTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcgtc                          >  7:232102/1‑149 (MQ=255)                                                                                                                            ggCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcgtcg                         >  7:270195/1‑149 (MQ=255)                                                                                                                             gCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcgtcg                         >  8:212097/1‑148 (MQ=255)                                                                                                                             gCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcgtcg                         >  4:219483/1‑148 (MQ=255)                                                                                                                              ccTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTa                       <  4:26150/149‑1 (MQ=255)                                                                                                                               ctctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcgtcg                         >  5:305928/1‑146 (MQ=255)                                                                                                                               ctctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg                      >  7:355110/1‑149 (MQ=255)                                                                                                                               ctctTCGCGTGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg                      <  1:208236/149‑1 (MQ=255)                                                                                                                               ctctTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg                      <  5:5150/149‑1 (MQ=255)                                                                                                                                tctTTGCGGGCACGCCAGCGAAGAGGCCGGTACTGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGAGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGc                     >  2:183906/1‑149 (MQ=255)                                                                                                                                tctTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTCGc                     >  3:264701/1‑149 (MQ=255)                                                                                                                                tctTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGc                     >  6:177729/1‑149 (MQ=255)                                                                                                                                 ctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGt                                                                     >  7:343660/1‑100 (MQ=38)                                                                                                                                 ctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGt                                                                     <  8:343660/100‑1 (MQ=38)                                                                                                                                     gcgGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTgcgc                <  3:219483/149‑1 (MQ=255)                                                                                                                                        ggCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCAcc             <  3:100027/149‑1 (MQ=255)                                                                                                                                             gCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTgg                               <  7:328317/126‑1 (MQ=255)                                                                                                                                             gCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCATgg                               >  8:328317/1‑126 (MQ=255)                                                                                                                                              cccGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTTCGCACCGAATcc       <  8:355110/149‑1 (MQ=255)                                                                                                                                              cccGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATcc       <  1:264655/149‑1 (MQ=255)                                                                                                                                              cccGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATcc       <  1:108577/149‑1 (MQ=255)                                                                                                                                              cccGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATcc       <  8:89384/149‑1 (MQ=255)                                                                                                                                               ccGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATcc       >  3:332042/1‑148 (MQ=255)                                                                                                                                                cgcgAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCa     <  8:232102/149‑1 (MQ=255)                                                                                                                                                cGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCa     >  1:306863/1‑149 (MQ=255)                                                                                                                                                  cAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCACt   <  5:177729/149‑1 (MQ=255)                                                                                                                                                   aaaGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCCTAGCTGCGAACCGAATCCCACTg  >  4:291504/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCACTG  >  NC_002947/1145302‑1145596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑