Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,253,429 G→C 6.3% intergenic (‑75/+32) PP_1096 ← / ← metG electron transport complex protein/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,253,4290GC 6.3% 200.8 / 4.1 63intergenic (‑75/+32)PP_1096/metGelectron transport complex protein/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base C (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

AGAGTGGCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCA  >  NC_002947/1253282‑1253570
                                                                                                                                                   |                                                                                                                                              
agagTGGCTGTCGGTGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCTGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGc                                                                                                                                               <  6:203819/149‑1 (MQ=255)
  agTGGCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAg                                                                                                                                             <  5:137453/149‑1 (MQ=255)
      gCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCg                                                                                                                                         >  3:172488/1‑149 (MQ=255)
         gTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGAGTAATTGTGGGAGCGGGCAGGCCGcga                                                                                                                                      >  8:284230/1‑147 (MQ=255)
          tCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTcc                                                                                                                                     >  1:74050/1‑149 (MQ=255)
           cGGAGCAGGCACAGGTGATTGATCAGTGCTGAGCTGATCAGGACCAGGATGTAGTCATTCATGAGAGGACGATAGCCGGCAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGCCTGCCCGCTcc                                                                                                                                     <  5:187964/148‑1 (MQ=255)
                   gCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGc                                                                                                                            <  2:46386/149‑1 (MQ=255)
                     acaGGTGATTGATCAGTGCGGCGCTGATCATGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGccc                                                                                                                          >  6:5740/1‑149 (MQ=255)
                     acaGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGccc                                                                                                                          >  4:253288/1‑149 (MQ=255)
                     acaGGTGATTGATCAGTGCGGCCCTGATCAGGACCAGGATGTAGTCATTCATGATATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGccc                                                                                                                          <  3:14685/149‑1 (MQ=255)
                             ttgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCg                                                                                                                  <  7:303186/149‑1 (MQ=255)
                             ttgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCg                                                                                                                  <  2:173454/149‑1 (MQ=255)
                              tgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCgg                                                                                                                 >  6:332645/1‑149 (MQ=255)
                                atCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGt                                                                                                                >  1:218790/1‑148 (MQ=255)
                                    gTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACtt                                                                                                           <  2:218790/149‑1 (MQ=255)
                                       cggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCa                                                                                                                       <  3:128831/134‑1 (MQ=255)
                                       cggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCa                                                                                                                       >  4:128831/1‑134 (MQ=255)
                                       cggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGa                                                                                                         <  3:115746/148‑1 (MQ=255)
                                         gcgcTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATgc                                                                                                      <  1:194193/149‑1 (MQ=255)
                                              gATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctgg                                                                                                 >  5:83923/1‑149 (MQ=255)
                                               aTCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggc                                                                                                >  2:135200/1‑149 (MQ=255)
                                               aTCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctg                                                                                                  <  4:357086/147‑1 (MQ=255)
                                                  aGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggcctg                                                                                             >  3:35879/1‑149 (MQ=255)
                                                    gACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCg                                                                                                                  >  6:122419/1‑126 (MQ=255)
                                                    gACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGGTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCg                                                                                                                  <  5:122419/126‑1 (MQ=255)
                                                       cAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTg                                                                                        >  3:129498/1‑149 (MQ=255)
                                                           aTGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgcgc                                                                                    <  7:251315/149‑1 (MQ=255)
                                                           aTGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgcgc                                                                                    <  5:238369/149‑1 (MQ=255)
                                                           aTGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgcgc                                                                                    <  4:129498/149‑1 (MQ=255)
                                                                gTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTg                                                                               >  5:260592/1‑149 (MQ=255)
                                                                   attcatGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑cc                                                                           <  6:260592/149‑1 (MQ=255)
                                                                    ttcatGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCg                                                                          >  8:274243/1‑149 (MQ=255)
                                                                     tcatGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCgg                                                                         >  3:189306/1‑149 (MQ=255)
                                                                       atGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGTAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGc                                                                       >  7:126847/1‑149 (MQ=255)
                                                                       atGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCggg                                                                        <  5:5740/148‑1 (MQ=255)
                                                                       atGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGc                                                                       >  7:81616/1‑149 (MQ=255)
                                                                            aTGACGATAGCCGGGAAGCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGGTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTcagc                                                                  <  6:346731/149‑1 (MQ=255)
                                                                                 gATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTa                                                             <  4:172488/149‑1 (MQ=255)
                                                                                   tAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGa                                                           <  3:253288/149‑1 (MQ=255)
                                                                                          gAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATttcttc                                                    <  8:243233/149‑1 (MQ=255)
                                                                                               ggggCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGc                                               <  6:83923/149‑1 (MQ=255)
                                                                                               ggggCGATTGTCCGGGTTTGACATGAAGGGGACTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGATACTTGAAGCGCTGGCCTGGCTTGGCCACGCTGT‑CCGGGCACAGCAGGTAGATTTCTTCACCGc                                               >  4:355766/1‑149 (MQ=255)
                                                                                                    gATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAggg                                          >  8:332355/1‑149 (MQ=255)
                                                                                                    gATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTACCACAATGGCCCGCAGGGCGGTTACTTGATTCGCTGCCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAggg                                          >  5:109820/1‑149 (MQ=255)
                                                                                                         tCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCgg                                      >  3:200897/1‑148 (MQ=255)
                                                                                                         tCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCgg                                      >  2:297533/1‑148 (MQ=255)
                                                                                                               tttGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCAt                               <  2:74050/149‑1 (MQ=255)
                                                                                                                  gACATGAAGGGGCCTGCGTAATTGTGGGAGCGTGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGa                             <  1:292589/148‑1 (MQ=255)
                                                                                                                  gACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGa                             <  7:274243/148‑1 (MQ=255)
                                                                                                                      tGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCAt                         <  1:294305/149‑1 (MQ=255)
                                                                                                                        aaGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGc                       <  6:137890/148‑1 (MQ=255)
                                                                                                                        aaGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGc                       <  6:143972/148‑1 (MQ=255)
                                                                                                                           gggCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTc                   >  6:221291/1‑149 (MQ=255)
                                                                                                                           gggCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAACGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTc                   <  5:332645/149‑1 (MQ=255)
                                                                                                                             gCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCg                  >  1:191869/1‑148 (MQ=255)
                                                                                                                               cTGCGTAATTGTGGGAGCTGGCTGGAGGCTCCCACAATGGCCCGGAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCTGac               <  8:126847/149‑1 (MQ=255)
                                                                                                                                  cGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACg            >  7:63221/1‑149 (MQ=255)
                                                                                                                                          tgGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAgcg    >  5:304854/1‑149 (MQ=255)
                                                                                                                                           gggAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGCCCATGCCTTCGGACACGCCAAAgcg    >  6:369471/1‑148 (MQ=255)
                                                                                                                                           gggAGCGGCCTGCCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAgcg    >  8:199247/1‑148 (MQ=255)
                                                                                                                                            ggAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGCCCCTGCCTTCGGCCACGCCAAAGCGCa  >  2:124043/1‑149 (MQ=255)
                                                                                                                                             gAGCGGCCTGCCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgc                                                                                      >  7:330376/1‑65 (MQ=37)
                                                                                                                                             gAGCGGCCTGCCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgc                                                                                      <  8:330376/65‑1 (MQ=37)
                                                                                                                                                   |                                                                                                                                              
AGAGTGGCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGT‑CCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCA  >  NC_002947/1253282‑1253570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑