BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,350,501	T→A	8.5%	K11N (AAA→AAT) ‡	PP_1176 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,350,501	0	T	A	8.5%	325.1 / 14.6	117	K11N (AAA→AAT) ‡	PP_1176	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (47/59); new base A (5/5); total (52/65)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 122 total aligned reads displayed.

GGAGAGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTCC  >  NC_002947/1350353‑1350649

 gagaGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAtt                                                                                                                                                      >  8:292928/1‑149 (MQ=255)

  agagGCGTTTCTTAAGATAAAGGGTTTTCGCTAAGTGTCGGTTCTTAAGCGCCGGTAAACGTTATGGGGATGTCCAAAAAAATGCAATGGCGGGATCCGTATGCATACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAtt                                                                                                                                                      <  1:272311/149‑1 (MQ=255)

     gCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                   <  7:242973/148‑1 (MQ=255)

      ccGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                   >  2:250040/1‑147 (MQ=255)

      ccGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCa                                                                                                                                                 <  6:214420/149‑1 (MQ=255)

      ccGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTACTGGTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                   >  6:84510/1‑147 (MQ=255)

            cTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCtt                                                                                                                                            <  4:140722/148‑1 (MQ=255)

             tGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCAGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGAAGCCttgt                                                                                                                                          >  7:149289/1‑149 (MQ=255)

              gACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                         >  7:236993/1‑149 (MQ=255)

               aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                         >  6:3401/1‑148 (MQ=255)

               aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                          <  8:52083/147‑1 (MQ=255)

               aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                          <  2:133841/147‑1 (MQ=255)

               aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttg                                                                                                                                           <  3:60458/146‑1 (MQ=255)

               aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttg                                                                                                                                           <  5:150128/146‑1 (MQ=255)

                 gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCTTTTGGCAGCCTGGTTGAt                                                                                                                                      >  8:341173/1‑149 (MQ=255)

                 gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGAt                                                                                                                                      <  8:296173/149‑1 (MQ=255)

                 gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttttatt                                                                                                                                      <  2:55846/149‑4 (MQ=255)

                 gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCAATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttt                                                                                                                                          <  4:13842/145‑3 (MQ=255)

                 gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCAATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttt                                                                                                                                          >  3:13842/1‑143 (MQ=255)

                       gggATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAg                                                                                                                                >  5:273289/1‑149 (MQ=255)

                        ggATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAACACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGc                                                                                                                               <  2:130791/149‑1 (MQ=255)

                        ggATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGc                                                                                                                               <  3:389005/149‑1 (MQ=255)

                        ggATGCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTACCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGc                                                                                                                               <  6:390187/149‑1 (MQ=255)

                              cGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGt                                                                                                                          <  2:48392/148‑1 (MQ=255)

                              cGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGt                                                                                                                          >  2:94535/1‑148 (MQ=255)

                               gCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGa                                                                                                                                       <  8:297943/134‑1 (MQ=255)

                               gCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGa                                                                                                                                       >  7:297943/1‑134 (MQ=255)

                                  aTGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCCGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                      >  8:201358/1‑148 (MQ=255)

                                   tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                      <  1:241794/147‑1 (MQ=255)

                                   tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                      <  7:371419/147‑1 (MQ=255)

                                   tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                      <  4:289603/147‑1 (MQ=255)

                                     tgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTcaatcaat                                                                                                                  <  7:292928/149‑1 (MQ=255)

                                      gAGCGTTCTTAAGCTCCTGAAAACGTTATCTCGATGTCCAAAAACAGTCAAGGTCGGCAGCCGTA‑TGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGAAGCCTTGTTGATGCTCAGCCCGGTcaatcaat                                                                                                                  <  5:209989/148‑1 (MQ=255)

                                      gAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCaatcaatc                                                                                                                 >  8:62322/1‑149 (MQ=255)

                                      gAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCaatcaatc                                                                                                                 >  7:290956/1‑149 (MQ=255)

                                       aGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATcc                                                                                                                <  1:250040/149‑1 (MQ=255)

                                        gCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAGACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTCCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATccc                                                                                                               <  7:296382/149‑1 (MQ=255)

                                           ttCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGc                                                                                                            <  6:273289/149‑1 (MQ=255)

                                                aaGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCaa                                                                                                        <  8:367712/148‑1 (MQ=255)

                                                    gcCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGccc                                                                                                   >  2:225895/1‑149 (MQ=255)

                                                      cGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCa                                                                                                 >  3:185731/1‑149 (MQ=255)

                                                      cGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCa                                                                                                 >  7:52191/1‑149 (MQ=255)

                                                        gAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCTTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAcc                                                                                               >  3:270640/1‑149 (MQ=255)

                                                        gAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTCGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAcc                                                                                               >  1:322517/1‑149 (MQ=255)

                                                         aaaaCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAccc                                                                                              >  7:18669/1‑149 (MQ=255)

                                                          aaaCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTGAATCAATCCCAGCTCAACGCCCCACCCg                                                                                             >  4:319574/1‑149 (MQ=255)

                                                           aaCGTTATCGCGATGTCCAAAAACAGGCAATGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGt                                                                                            <  7:96004/149‑1 (MQ=255)

                                                            aCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTc                                                                                           >  1:61506/1‑149 (MQ=255)

                                                              gTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCt                                                                                          >  1:327010/1‑148 (MQ=255)

                                                              gTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTACTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCt                                                                                          >  4:154975/1‑148 (MQ=255)

                                                               ttATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGTCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGa                                                                                        >  2:84613/1‑149 (MQ=255)

                                                                 aTCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGAt                                                                                       <  8:234741/148‑1 (MQ=255)

                                                                    gcgATGTGCAATATCAGGCAAGGTCGGCTGACGTA‑GGTAACGTCCTGTTCTGCTTCTGCCAGGCAAAAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATAAATCCCATCTGAACGCCCCACCCGTCTGATACt                                                                                    <  8:145993/148‑1 (MQ=255)

                                                                    gcgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTc                                                                                   <  1:225866/149‑1 (MQ=255)

                                                                     cgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTc                                                                                   >  6:399820/1‑148 (MQ=255)

                                                                     cgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCg                                                                                  <  8:18669/149‑1 (MQ=255)

                                                                      gATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGGTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGa                                                                                 <  5:84510/149‑1 (MQ=255)

                                                                       aTGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCTTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGAt                                                                                <  4:270640/149‑1 (MQ=255)

                                                                       aTGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGAt                                                                                <  7:62322/149‑1 (MQ=255)

                                                                            cAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCacac                                                                           >  2:221016/1‑149 (MQ=255)

                                                                              aaaaCAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACAcgc                                                                         <  4:99128/149‑1 (MQ=255)

                                                                                 aCAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGtc                                                                      <  1:225895/149‑1 (MQ=255)

                                                                                   aGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGtctc                                                                    <  8:236993/149‑1 (MQ=255)

                                                                                     gCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCa                                                                   >  7:146368/1‑148 (MQ=255)

                                                                                        ctgTCGGCAGCCGTA‑GGAAAAGTCCTGTTCATCTTCTGCCAGGCAATAACAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTAGATCACACGCGTCTCAaag‑a                                                              <  8:149289/147‑1 (MQ=255)

                                                                                         ggTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaag‑aa                                                             <  6:65652/149‑1 (MQ=255)

                                                                                             ggCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGTCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGttc                                                         <  1:84613/149‑1 (MQ=255)

                                                                                                  ccGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTtctc                                                    <  7:287708/149‑1 (MQ=255)

                                                                                                    gTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCct                                                  >  6:96357/1‑149 (MQ=255)

                                                                                                        ggAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCcttct                                               <  5:399820/149‑1 (MQ=255)

                                                                                                        ggAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCcttct                                               <  1:94535/149‑1 (MQ=255)

                                                                                                          aaaCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCttcttc                                             <  5:96357/149‑1 (MQ=255)

                                                                                                            aCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCttcttc                                             >  3:327137/1‑147 (MQ=255)

                                                                                                              gTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGATCAACGCCCCACCCGTCTGATACTCGAACACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGt                                         >  5:38167/1‑149 (MQ=255)

                                                                                                               tCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTc                                        >  5:334565/1‑149 (MQ=255)

                                                                                                                ccTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTc                                        >  4:237448/1‑148 (MQ=255)

                                                                                                                      ctgcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCCCCCGTCTGATACTCGATCCCCCGCGTCGCAAAG‑AAGTTCTTCGCCTGCTGCAAGGCCAGGa                                   >  7:245229/1‑147 (MQ=255)

                                                                                                                         cttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGAtc                                 <  4:127818/146‑1 (MQ=255)

                                                                                                                          ttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGc                             >  1:12878/1‑149 (MQ=255)

                                                                                                                                    cAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   <  8:52191/149‑1 (MQ=255)

                                                                                                                                    cAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   <  2:257364/149‑1 (MQ=255)

                                                                                                                                    cAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   <  2:327010/149‑1 (MQ=255)

                                                                                                                                         aaaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTtcttct                                                     >  6:125842/1‑110 (MQ=255)

                                                                                                                                         aaaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTtcttct                                                     <  5:125842/110‑1 (MQ=255)

                                                                                                                                          caaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTtctc                                                    >  7:255902/2‑110 (MQ=255)

                                                                                                                                          aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACgg             <  3:58780/149‑1 (MQ=255)

                                                                                                                                          aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACgg             >  4:58780/1‑149 (MQ=255)

                                                                                                                                          aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGATCAACGCCCCACCCGTCTGATACTCGATCACACCCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCCCTCATCCATGGGAACgg             >  8:28875/1‑149 (MQ=255)

                                                                                                                                          aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCACCCCGGTCAATCAATCCCTACTCAACGCCCCACCCGTCTCATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCGATCATCTCGCTCATCCATGGGAACgg             <  2:223114/149‑1 (MQ=255)

                                                                                                                                           aaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   >  2:306722/1‑142 (MQ=255)

                                                                                                                                           aaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   <  1:306722/142‑1 (MQ=255)

                                                                                                                                            aGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGTCAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACggg            <  1:294267/149‑1 (MQ=255)

                                                                                                                                            aGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGt           >  8:242824/1‑149 (MQ=255)

                                                                                                                                             ggCTTCCAGTTGGCAGCCTTGTTGATGCTCAGCCCGTTCAATCAATCACAGCTCAACGCCCCACCCGTCTGATACTCGATCACACTCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGtt          <  4:32296/149‑1 (MQ=255)

                                                                                                                                             ggCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAACCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGtt          <  3:343395/149‑1 (MQ=255)

                                                                                                                                             ggCTGCCAAATGGCAGCCTTGTTGCTGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGtt          >  8:124974/1‑149 (MQ=255)

                                                                                                                                               ggggcctttGGCCGCCTTGTTGATGCTCCGCCCCGTCCATCACTCCCAGCTCCACGCCCCCCCCGTCTGCTACTCGCTCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATCATCTCGCTCATCCCTGGGAACGGGTTgg        <  8:245229/143‑1 (MQ=255)

                                                                                                                                               cTGCCATTTGGAAGCCTTTTTGATGCTCAGCCCGGTCATTCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCTTCTCAATA‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTgg        <  1:304182/149‑1 (MQ=255)

                                                                                                                                                  ccATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTa      <  2:12878/148‑1 (MQ=255)

                                                                                                                                                     tttGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAATTACATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTcc  >  3:225108/1‑149 (MQ=255)

GGAGAGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTCC  >  NC_002947/1350353‑1350649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`