BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,350,502	T→A	8.5%	K11I (AAA→ATA) ‡	PP_1176 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,350,502	0	T	A	8.5%	339.5 / 15.7	117	K11I (AAA→ATA) ‡	PP_1176	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (47/60); new base A (5/5); total (52/65)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

Only 100 of 121 total aligned reads displayed.

GAGAGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTCC  >  NC_002947/1350354‑1350649

gagaGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAtt                                                                                                                                                      >  8:292928/1‑149 (MQ=255)

 agagGCGTTTCTTAAGATAAAGGGTTTTCGCTAAGTGTCGGTTCTTAAGCGCCGGTAAACGTTATGGGGATGTCCAAAAAAATGCAATGGCGGGATCCGTATGCATACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAtt                                                                                                                                                      <  1:272311/149‑1 (MQ=255)

   agCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                   <  6:339008/149‑1 (MQ=255)

    gCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                   <  7:242973/148‑1 (MQ=255)

     ccGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCa                                                                                                                                                 <  7:28107/149‑1 (MQ=255)

     ccGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTACTGGTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                   >  6:84510/1‑147 (MQ=255)

        tttCTGACGAGAACGTGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGcc                                                                                                                                              <  2:120816/149‑1 (MQ=255)

             gACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                         >  7:236993/1‑149 (MQ=255)

              aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                         >  6:3401/1‑148 (MQ=255)

              aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                          <  8:52083/147‑1 (MQ=255)

              aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                          <  2:133841/147‑1 (MQ=255)

              aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttg                                                                                                                                           <  3:60458/146‑1 (MQ=255)

              aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttg                                                                                                                                           <  5:150128/146‑1 (MQ=255)

                gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCTTTTGGCAGCCTGGTTGAt                                                                                                                                      >  8:341173/1‑149 (MQ=255)

                gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGAt                                                                                                                                      <  8:296173/149‑1 (MQ=255)

                gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttttatt                                                                                                                                      <  4:111855/149‑4 (MQ=255)

                gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttttatt                                                                                                                                      <  2:55846/149‑4 (MQ=255)

                gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCAATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttt                                                                                                                                          <  4:13842/145‑3 (MQ=255)

                gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCAATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttt                                                                                                                                          >  3:13842/1‑143 (MQ=255)

                   aaCGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGc                                                                                                                                    >  7:367712/1‑148 (MQ=255)

                     cGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCt                                                                                                                                   >  8:20445/1‑147 (MQ=255)

                      gggATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAg                                                                                                                                >  5:273289/1‑149 (MQ=255)

                       ggATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAACACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGc                                                                                                                               <  2:130791/149‑1 (MQ=255)

                       ggATGCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTACCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGc                                                                                                                               <  6:390187/149‑1 (MQ=255)

                             cGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGt                                                                                                                          >  2:94535/1‑148 (MQ=255)

                              gCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGa                                                                                                                                       <  8:297943/134‑1 (MQ=255)

                              gCTATCTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGTTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTATGCCAGGCAATAAAAAGGATGCCATTTGGCAGCCTTGTTGATGCTCAGCACGGtc                                                                                                                         >  3:32296/1‑148 (MQ=255)

                                  tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                      <  1:241794/147‑1 (MQ=255)

                                  tgtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                      <  7:371419/147‑1 (MQ=255)

                                    tgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTcaatcaat                                                                                                                  <  7:292928/149‑1 (MQ=255)

                                     gAGCGTTCTTAAGCTCCTGAAAACGTTATCTCGATGTCCAAAAACAGTCAAGGTCGGCAGCCGTA‑TGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGAAGCCTTGTTGATGCTCAGCCCGGTcaatcaat                                                                                                                  <  5:209989/148‑1 (MQ=255)

                                     gAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCaatcaatc                                                                                                                 >  8:62322/1‑149 (MQ=255)

                                     gAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCaatcaatc                                                                                                                 >  7:290956/1‑149 (MQ=255)

                                      aGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATcc                                                                                                                >  3:127818/1‑149 (MQ=255)

                                      aGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATcc                                                                                                                <  1:250040/149‑1 (MQ=255)

                                       gCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAGACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTCCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATccc                                                                                                               <  7:296382/149‑1 (MQ=255)

                                          ttCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGc                                                                                                            <  6:273289/149‑1 (MQ=255)

                                            cTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCt                                                                                                           >  7:96486/1‑148 (MQ=255)

                                               aaGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCaa                                                                                                        <  8:367712/148‑1 (MQ=255)

                                                   gcCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGccc                                                                                                   >  2:225895/1‑149 (MQ=255)

                                                     cGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCa                                                                                                 >  7:52191/1‑149 (MQ=255)

                                                       gAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCTTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAcc                                                                                               >  3:270640/1‑149 (MQ=255)

                                                       gAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTCGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAcc                                                                                               >  1:322517/1‑149 (MQ=255)

                                                        aaaaCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAccc                                                                                              >  7:18669/1‑149 (MQ=255)

                                                         aaaCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTGAATCAATCCCAGCTCAACGCCCCACCCg                                                                                             >  4:319574/1‑149 (MQ=255)

                                                           aCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTc                                                                                           >  1:61506/1‑149 (MQ=255)

                                                             gTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCt                                                                                          >  1:327010/1‑148 (MQ=255)

                                                             gTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTACTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCt                                                                                          >  4:154975/1‑148 (MQ=255)

                                                              ttATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGTCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGa                                                                                        >  2:84613/1‑149 (MQ=255)

                                                                aTCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGAt                                                                                       <  8:234741/148‑1 (MQ=255)

                                                                   gcgATGTGCAATATCAGGCAAGGTCGGCTGACGTA‑GGTAACGTCCTGTTCTGCTTCTGCCAGGCAAAAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATAAATCCCATCTGAACGCCCCACCCGTCTGATACt                                                                                    <  8:145993/148‑1 (MQ=255)

                                                                   gcgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTc                                                                                   <  1:225866/149‑1 (MQ=255)

                                                                    cgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCg                                                                                  <  8:18669/149‑1 (MQ=255)

                                                                     gATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGGTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGa                                                                                 <  5:84510/149‑1 (MQ=255)

                                                                      aTGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCTTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGAt                                                                                <  4:270640/149‑1 (MQ=255)

                                                                      aTGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGAt                                                                                <  7:62322/149‑1 (MQ=255)

                                                                           cAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCacac                                                                           >  2:221016/1‑149 (MQ=255)

                                                                           cAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCacac                                                                           >  4:343395/1‑149 (MQ=255)

                                                                             aaaaCAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACAcgc                                                                         <  4:99128/149‑1 (MQ=255)

                                                                                aCAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGtc                                                                      <  1:225895/149‑1 (MQ=255)

                                                                                  aGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGtctc                                                                    <  8:236993/149‑1 (MQ=255)

                                                                                    gCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCa                                                                   >  7:146368/1‑148 (MQ=255)

                                                                                       ctgTCGGCAGCCGTA‑GGAAAAGTCCTGTTCATCTTCTGCCAGGCAATAACAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTAGATCACACGCGTCTCAaag‑a                                                              <  8:149289/147‑1 (MQ=255)

                                                                                        ggTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaag‑aa                                                             <  6:65652/149‑1 (MQ=255)

                                                                                            ggCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGTCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGttc                                                         <  1:84613/149‑1 (MQ=255)

                                                                                                 ccGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTtctc                                                    <  7:287708/149‑1 (MQ=255)

                                                                                                   gTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCct                                                  >  6:96357/1‑149 (MQ=255)

                                                                                                       ggAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCcttct                                               <  5:399820/149‑1 (MQ=255)

                                                                                                       ggAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCcttct                                               <  1:94535/149‑1 (MQ=255)

                                                                                                         aaaCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCttcttc                                             <  5:96357/149‑1 (MQ=255)

                                                                                                           aCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCttcttc                                             >  3:327137/1‑147 (MQ=255)

                                                                                                              tCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTc                                        >  5:334565/1‑149 (MQ=255)

                                                                                                               ccTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTc                                        >  4:237448/1‑148 (MQ=255)

                                                                                                                   ttctgcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCAt                                     <  2:322517/147‑1 (MQ=255)

                                                                                                                   ttctgcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATg                                    <  7:20445/148‑1 (MQ=255)

                                                                                                                     ctgcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCCCCCGTCTGATACTCGATCCCCCGCGTCGCAAAG‑AAGTTCTTCGCCTGCTGCAAGGCCAGGa                                   >  7:245229/1‑147 (MQ=255)

                                                                                                                        cttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGAtc                                 <  4:127818/146‑1 (MQ=255)

                                                                                                                         ttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGc                             >  1:12878/1‑149 (MQ=255)

                                                                                                                                   cAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   <  2:257364/149‑1 (MQ=255)

                                                                                                                                   cAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   <  8:52191/149‑1 (MQ=255)

                                                                                                                                   cAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   <  2:327010/149‑1 (MQ=255)

                                                                                                                                    atcaacaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   >  6:79630/7‑148 (MQ=255)

                                                                                                                                        aaaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTtcttct                                                     >  6:125842/1‑110 (MQ=255)

                                                                                                                                         aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACgg             <  3:58780/149‑1 (MQ=255)

                                                                                                                                         aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACgg             >  4:58780/1‑149 (MQ=255)

                                                                                                                                         aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGATCAACGCCCCACCCGTCTGATACTCGATCACACCCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCCCTCATCCATGGGAACgg             >  8:28875/1‑149 (MQ=255)

                                                                                                                                         aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCACCCCGGTCAATCAATCCCTACTCAACGCCCCACCCGTCTCATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCGATCATCTCGCTCATCCATGGGAACgg             <  2:223114/149‑1 (MQ=255)

                                                                                                                                          aaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   >  2:306722/1‑142 (MQ=255)

                                                                                                                                          aaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                   <  1:306722/142‑1 (MQ=255)

                                                                                                                                           aGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGTCAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACggg            <  1:294267/149‑1 (MQ=255)

                                                                                                                                           aGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGt           >  8:242824/1‑149 (MQ=255)

                                                                                                                                           aGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGt           <  5:79630/149‑1 (MQ=255)

                                                                                                                                            ggCTTCCAGTTGGCAGCCTTGTTGATGCTCAGCCCGTTCAATCAATCACAGCTCAACGCCCCACCCGTCTGATACTCGATCACACTCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGtt          <  4:32296/149‑1 (MQ=255)

                                                                                                                                            ggCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAACCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGtt          <  3:343395/149‑1 (MQ=255)

                                                                                                                                            ggCTGCCAAATGGCAGCCTTGTTGCTGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGtt          >  8:124974/1‑149 (MQ=255)

                                                                                                                                             gCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATAACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATCATCTCGCTCATCCATGGGAACGGGTTg         >  5:204821/1‑149 (MQ=255)

                                                                                                                                              ggggcctttGGCCGCCTTGTTGATGCTCCGCCCCGTCCATCACTCCCAGCTCCACGCCCCCCCCGTCTGCTACTCGCTCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATCATCTCGCTCATCCCTGGGAACGGGTTgg        <  8:245229/143‑1 (MQ=255)

                                                                                                                                              cTGCCATTTGGAAGCCTTTTTGATGCTCAGCCCGGTCATTCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCTTCTCAATA‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTgg        <  1:304182/149‑1 (MQ=255)

                                                                                                                                                 ccATTTGGCAGGCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTa      <  4:185731/148‑1 (MQ=255)

                                                                                                                                                    tttGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAATTACATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTcc  >  3:225108/1‑149 (MQ=255)

GAGAGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTA‑GGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAG‑AAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTCC  >  NC_002947/1350354‑1350649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`