Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,356,019 G→C 6.9% intergenic (+286/‑106) PP_1180 → / → PP_1181 hypothetical protein/two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,356,0190GC 6.9% 273.6 / 8.6 87intergenic (+286/‑106)PP_1180/PP_1181hypothetical protein/two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (35/46);  new base C (2/4);  total (37/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01

TATTCGCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGAC  >  NC_002947/1355871‑1356166
                                                                                                                                                     |                                                                                                                                                    
tATTCGCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTgg                                                                                                                                                      <  5:191341/149‑1 (MQ=255)
 aTTCGCAGTAGGCCTTCTCCCAAGTGGTTAGCAGTAAATTTCTGCAACGTCAAGCCCTAGGGCAGTTTTTTTTGCACGCGAAGCCTCTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTCAAGATCTGGGGTG‑CCTgg                                                                                                                                                      >  2:107498/1‑148 (MQ=255)
  ttCGCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTCTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTggg                                                                                                                                                     <  5:80973/148‑1 (MQ=255)
   tCGCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTgg                                                                                                                                                      >  5:19262/1‑146 (MQ=255)
     gCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTgg                                                                                                                                                      >  3:283988/1‑144 (MQ=255)
     gCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTgg                                                                                                                                                      >  2:128544/1‑144 (MQ=255)
     gCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGgaga                                                                                                                                                  <  8:120493/148‑1 (MQ=255)
     gCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGAt                                                                                                                                                 <  2:165935/149‑1 (MQ=255)
       tGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGTTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTCGGAGATc                                                                                                                                                >  3:323515/1‑148 (MQ=255)
         tAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCg                                                                                                                                               <  6:289565/147‑1 (MQ=255)
           ggCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGc                                                                                                                                            <  2:57845/148‑1 (MQ=255)
           ggCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGCGAGACCGAGcg                                                                                                                                           >  2:331586/1‑148 (MQ=255)
                 gtgCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCAccgccc                                                                                                                                     >  7:193996/1‑149 (MQ=255)
                 gtgCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCAccgcc                                                                                                                                      <  7:63149/148‑1 (MQ=255)
                       aGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgggg                                                                                                                               <  6:19262/149‑1 (MQ=255)
                       aGTGGTTTGCAGTATTTTTCCGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgggg                                                                                                                               <  7:316201/149‑1 (MQ=255)
                        gTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGgc                                                                                                                              <  1:10731/149‑1 (MQ=255)
                         tGGTTTGCAGTATTTTTCTGCACCGGGAACCCCTTGGGCAGTTTTTTTTGGGCCCGAAGCCTGTCACAAAAAACGGTTCCGTCGA‑GGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGgcgc                                                                                                                            <  5:173616/149‑1 (MQ=255)
                           gTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgg                                                                                                                                 >  8:50954/1‑143 (MQ=255)
                           gTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgg                                                                                                                                 <  7:50954/143‑1 (MQ=255)
                           gTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCt                                                                                                                           <  4:123038/149‑1 (MQ=255)
                            tttGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTc                                                                                                                          >  3:197767/1‑149 (MQ=255)
                              tGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCg                                                                                                                         <  6:202200/148‑1 (MQ=255)
                               gCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGt                                                                                                                       >  1:332118/1‑149 (MQ=255)
                                cAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGAtc                                                                                                                      <  6:154083/149‑1 (MQ=38)
                                 aGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGtct                                                                                                                     >  5:122315/1‑149 (MQ=255)
                                   tATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCg                                                                                                                         >  2:91069/1‑143 (MQ=255)
                                   tATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCg                                                                                                                         <  1:91069/143‑1 (MQ=255)
                                   tATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCg                                                                                                                   >  5:389433/1‑149 (MQ=255)
                                     tttttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGAtctccc                                                                                                                  <  1:331586/148‑3 (MQ=37)
                                     tttttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCa                                                                                                                 <  3:158593/149‑1 (MQ=37)
                                       tttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAg                                                                                                                <  2:334088/148‑1 (MQ=255)
                                        tacaccACCGTGAAGCCCTTGGGCCGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGGTCAGACGAGGGGGCGGTAGGGCGTGGCTGAAGAGCTGGGGGG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGGCTCGCAgg                                                                                                               <  5:97608/143‑1 (MQ=255)
                                           tGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGccc                                                                                                           >  5:256471/1‑149 (MQ=255)
                                            gCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGcccc                                                                                                          >  7:83331/1‑149 (MQ=255)
                                             cACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGccccc                                                                                                         >  7:234599/1‑149 (MQ=255)
                                             cACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGccc                                                                                                           <  6:357637/147‑1 (MQ=255)
                                             cACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGGGCCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCAggcac                                                                                                            <  8:27898/147‑4 (MQ=21)
                                               ccGTGAAGCCCGTGGGCAGTGTGTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCa                                                                                                       <  6:224517/149‑1 (MQ=255)
                                                cGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCAt                                                                                                      <  1:128544/149‑1 (MQ=255)
                                                cGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCAt                                                                                                      <  5:124736/149‑1 (MQ=255)
                                                 gTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATc                                                                                                     <  3:165628/149‑1 (MQ=255)
                                                  tGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATc                                                                                                     >  3:226860/1‑148 (MQ=255)
                                                   gAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCt                                                                                                    >  3:76916/1‑148 (MQ=255)
                                                     aGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTAc                                                                                                  <  6:243927/148‑1 (MQ=255)
                                                       accTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACAtt                                                                                               <  8:83331/148‑1 (MQ=255)
                                                        ccTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACAtt                                                                                               >  7:51349/1‑148 (MQ=255)
                                                          ttGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCCCCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCa                                                                                             >  1:244774/1‑148 (MQ=255)
                                                            gggCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATa                                                                                           <  8:51349/148‑1 (MQ=255)
                                                            gcgCACTTATTTTTGGGAGCGAAGCCTCTCACAAAAAAAGCAACAGTCTAAGGCCCGCTAGCGAGTGTCTCAATCACTTGGGTG‑CCTGGGAGATCGAGCACAGCCAGCGGACCGCAAGGACTCCCAGGCCACCCCAACTACAAACATa                                                                                           >  8:249862/3‑148 (MQ=255)
                                                             ggCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATAc                                                                                          <  4:164707/148‑1 (MQ=255)
                                                               cAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATActc                                                                                        >  7:31284/1‑148 (MQ=255)
                                                                aGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACtctc                                                                                      <  1:393649/149‑1 (MQ=255)
                                                                aGTTTTTTTTGGGAGCGAAGACTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGAGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGACCCCCATCTACATTCAAGctct                                                                                       >  4:132366/1‑148 (MQ=255)
                                                                 gTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCa                                                                                     <  2:328267/149‑1 (MQ=255)
                                                                  ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATTGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                    <  6:389433/149‑1 (MQ=255)
                                                                  ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTCCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                    >  4:184369/1‑149 (MQ=255)
                                                                       tttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGc                                                                               <  4:197767/148‑1 (MQ=255)
                                                                          gggCGCGATGCGTGTCACAATAAACGGTTCAGTCGTGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTTGGAGATCGAGCACCGCCCGCGGGGAGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCt                                                                            <  2:244774/148‑1 (MQ=255)
                                                                           ggCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGa                                                                          <  6:122315/149‑1 (MQ=255)
                                                                            gcgcGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGac                                                                         <  2:153979/149‑1 (MQ=255)
                                                                            cagcgAAGCCTGTCACAAAAAACGGTACAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCCGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGa                                                                          >  4:266443/3‑148 (MQ=255)
                                                                             cgcgAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGaca                                                                        <  1:184182/149‑1 (MQ=255)
                                                                              gcgAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGacac                                                                       >  3:366741/1‑149 (MQ=255)
                                                                                gAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGa                                                                     <  4:283988/149‑1 (MQ=255)
                                                                                    ccTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCGCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATccc                                                                 <  4:323515/149‑1 (MQ=255)
                                                                                        tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCt                                                                            <  2:168084/134‑1 (MQ=255)
                                                                                        tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCt                                                                            >  1:168084/1‑134 (MQ=255)
                                                                                        tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCaa                                                               <  4:366741/147‑1 (MQ=255)
                                                                                        tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAg                                                              >  2:165405/1‑148 (MQ=255)
                                                                                          acaAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgggg                                                                                                                               >  2:92918/1‑82 (MQ=255)
                                                                                            aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGCGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATACATACTCTCAGC‑AGCCCTGACACGTTCCCACGaaca                                                          >  8:131795/1‑148 (MQ=39)
                                                                                             aaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGaacaac                                                        <  1:165405/149‑1 (MQ=255)
                                                                                             aaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGaacaac                                                        <  4:227270/149‑1 (MQ=255)
                                                                                                aaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCATGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAg                                                     >  7:130337/1‑149 (MQ=255)
                                                                                                aaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAg                                                     <  4:226860/149‑1 (MQ=255)
                                                                                                        aGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGc                                             >  7:157428/1‑149 (MQ=255)
                                                                                                            gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACACTCCGC‑TGCCCTGCCACGATCCCAAGAACAACAAGGCCAACGCCATg                                         >  8:255720/1‑149 (MQ=255)
                                                                                                            gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCGACATTCATACTCTCAGC‑TGCCCTGACACCATCCCAAGAACAACAAGGCCAAAGCCATg                                         >  2:206492/1‑149 (MQ=255)
                                                                                                                  gCGGTAGGGCGTGTCTGAAGATCTGGTGTG‑CCTGGTAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGaccac                                   <  3:132366/149‑1 (MQ=255)
                                                                                                                    ggTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATCGACCAccc                                 >  8:342849/1‑149 (MQ=255)
                                                                                                                     gTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                 >  5:346189/1‑148 (MQ=255)
                                                                                                                             tgtCTGAAGATCTGGGGTG‑CCTGGGAGATCGTGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCATGCCCTGACTCGATCCCTAGAACAACAAGGCCAAAGCCATGGACCACCCCATAcccc                         <  1:107498/149‑1 (MQ=255)
                                                                                                                                cTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCAt                     >  8:112963/1‑149 (MQ=255)
                                                                                                                                 tGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCTATGTTCATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                    <  7:255720/149‑1 (MQ=255)
                                                                                                                                   aaGATCTGGGGTG‑CCTGGGAGATCGAGCAGCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAACCATGGACCACCCCATAGCCCGCATc                    <  4:126225/147‑1 (MQ=255)
                                                                                                                                   aaGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                    <  6:346189/147‑1 (MQ=255)
                                                                                                                                      aTCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATCCCCCGCATCCTCAt               >  6:113813/1‑149 (MQ=255)
                                                                                                                                       tCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtc              >  8:364654/1‑149 (MQ=255)
                                                                                                                                           gggTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATTGACCACCCCATACCCCGCATCCTCAtcgt            <  7:342849/147‑1 (MQ=255)
                                                                                                                                             gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtcg          >  4:53108/5‑147 (MQ=21)
                                                                                                                                               g‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAga      <  2:366669/149‑1 (MQ=255)
                                                                                                                                                   tGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacga   >  2:346751/1‑149 (MQ=255)
                                                                                                                                                    gggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac  >  5:347225/1‑149 (MQ=255)
                                                                                                                                                     ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac  <  8:157428/148‑1 (MQ=255)
                                                                                                                                                     ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac  <  8:31284/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TATTCGCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGAC  >  NC_002947/1355871‑1356166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑