Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,356,024 T→C 7.7% intergenic (+291/‑101) PP_1180 → / → PP_1181 hypothetical protein/two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,356,0240TC 7.7% 256.3 / 9.0 91intergenic (+291/‑101)PP_1180/PP_1181hypothetical protein/two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base T (36/48);  new base C (2/5);  total (38/53)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

GCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGC  >  NC_002947/1355876‑1356170
                                                                                                                                                     |                                                                                                                                                   
gCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGAt                                                                                                                                                     <  2:165935/149‑1 (MQ=255)
  tGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGTTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTCGGAGATc                                                                                                                                                    >  3:323515/1‑148 (MQ=255)
    tAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCg                                                                                                                                                   <  6:289565/147‑1 (MQ=255)
      ggCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGc                                                                                                                                                <  2:57845/148‑1 (MQ=255)
      ggCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGCGAGACCGAGcg                                                                                                                                               >  2:331586/1‑148 (MQ=255)
            gtgCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCAccgccc                                                                                                                                         >  7:193996/1‑149 (MQ=255)
            gtgCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCAccgcc                                                                                                                                          <  7:63149/148‑1 (MQ=255)
                  aGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgggg                                                                                                                                   <  6:19262/149‑1 (MQ=255)
                  aGTGGTTTGCAGTATTTTTCCGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgggg                                                                                                                                   <  7:316201/149‑1 (MQ=255)
                   gTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGgc                                                                                                                                  <  1:10731/149‑1 (MQ=255)
                    tGGTTTGCAGTATTTTTCTGCACCGGGAACCCCTTGGGCAGTTTTTTTTGGGCCCGAAGCCTGTCACAAAAAACGGTTCCGTCGA‑GGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGgcgc                                                                                                                                <  5:173616/149‑1 (MQ=255)
                      gTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgg                                                                                                                                     <  7:50954/143‑1 (MQ=255)
                      gTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgg                                                                                                                                     >  8:50954/1‑143 (MQ=255)
                      gTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCt                                                                                                                               <  4:123038/149‑1 (MQ=255)
                       tttGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTc                                                                                                                              >  3:197767/1‑149 (MQ=255)
                         tGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCg                                                                                                                             <  6:202200/148‑1 (MQ=255)
                          gCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGt                                                                                                                           >  1:332118/1‑149 (MQ=255)
                           cAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGAtc                                                                                                                          <  6:154083/149‑1 (MQ=38)
                            aGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGtct                                                                                                                         >  5:122315/1‑149 (MQ=255)
                              tATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCg                                                                                                                             >  2:91069/1‑143 (MQ=255)
                              tATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCg                                                                                                                             <  1:91069/143‑1 (MQ=255)
                              tATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCg                                                                                                                       >  5:389433/1‑149 (MQ=255)
                                tttttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGAtctccc                                                                                                                      <  1:331586/148‑3 (MQ=37)
                                tttttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCa                                                                                                                     <  3:158593/149‑1 (MQ=37)
                                  tttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAg                                                                                                                    <  2:334088/148‑1 (MQ=255)
                                   tacaccACCGTGAAGCCCTTGGGCCGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGGTCAGACGAGGGGGCGGTAGGGCGTGGCTGAAGAGCTGGGGGG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGGCTCGCAgg                                                                                                                   <  5:97608/143‑1 (MQ=255)
                                      tGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGccc                                                                                                               >  5:256471/1‑149 (MQ=255)
                                       gCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGcccc                                                                                                              >  7:83331/1‑149 (MQ=255)
                                        cACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGccccc                                                                                                             >  7:234599/1‑149 (MQ=255)
                                        cACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGccc                                                                                                               <  6:357637/147‑1 (MQ=255)
                                        cACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGGGCCCTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCAggcac                                                                                                                <  8:27898/147‑4 (MQ=21)
                                          ccGTGAAGCCCGTGGGCAGTGTGTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCa                                                                                                           <  6:224517/149‑1 (MQ=255)
                                           cGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCAt                                                                                                          <  1:128544/149‑1 (MQ=255)
                                           cGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCAt                                                                                                          <  5:124736/149‑1 (MQ=255)
                                            gTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATc                                                                                                         <  3:165628/149‑1 (MQ=255)
                                             tGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATc                                                                                                         >  3:226860/1‑148 (MQ=255)
                                              gAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCt                                                                                                        >  3:76916/1‑148 (MQ=255)
                                                aGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTAc                                                                                                      <  6:243927/148‑1 (MQ=255)
                                                  accTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACAtt                                                                                                   <  8:83331/148‑1 (MQ=255)
                                                   ccTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACAtt                                                                                                   >  7:51349/1‑148 (MQ=255)
                                                     ttGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCCCCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCa                                                                                                 >  1:244774/1‑148 (MQ=255)
                                                       gggCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATa                                                                                               <  8:51349/148‑1 (MQ=255)
                                                       gcgCACTTATTTTTGGGAGCGAAGCCTCTCACAAAAAAAGCAACAGTCTAAGGCCCGCTAGCGAGTGTCTCAATCACTTGGGTG‑CCTGGGAGATCGAGCACAGCCAGCGGACCGCAAGGACTCCCAGGCCACCCCAACTACAAACATa                                                                                               >  8:249862/3‑148 (MQ=255)
                                                        ggCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATAc                                                                                              <  4:164707/148‑1 (MQ=255)
                                                          cAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATActc                                                                                            >  7:31284/1‑148 (MQ=255)
                                                           aGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACtctc                                                                                          <  1:393649/149‑1 (MQ=255)
                                                           aGTTTTTTTTGGGAGCGAAGACTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGAGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGACCCCCATCTACATTCAAGctct                                                                                           >  4:132366/1‑148 (MQ=255)
                                                            gTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCa                                                                                         <  2:328267/149‑1 (MQ=255)
                                                             ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATTGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                        <  6:389433/149‑1 (MQ=255)
                                                             ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTCCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                        >  4:184369/1‑149 (MQ=255)
                                                                  tttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGc                                                                                   <  4:197767/148‑1 (MQ=255)
                                                                     gggCGCGATGCGTGTCACAATAAACGGTTCAGTCGTGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTTGGAGATCGAGCACCGCCCGCGGGGAGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCt                                                                                <  2:244774/148‑1 (MQ=255)
                                                                      ggCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGa                                                                              <  6:122315/149‑1 (MQ=255)
                                                                       gcgcGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGac                                                                             <  2:153979/149‑1 (MQ=255)
                                                                       cagcgAAGCCTGTCACAAAAAACGGTACAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCCGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGa                                                                              >  4:266443/3‑148 (MQ=255)
                                                                        cgcgAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGaca                                                                            <  1:184182/149‑1 (MQ=255)
                                                                         gcgAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGacac                                                                           >  3:366741/1‑149 (MQ=255)
                                                                           gAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGa                                                                         <  4:283988/149‑1 (MQ=255)
                                                                               ccTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCGCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATccc                                                                     <  4:323515/149‑1 (MQ=255)
                                                                                   tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCt                                                                                >  1:168084/1‑134 (MQ=255)
                                                                                   tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCt                                                                                <  2:168084/134‑1 (MQ=255)
                                                                                   tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCaa                                                                   <  4:366741/147‑1 (MQ=255)
                                                                                   tCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAg                                                                  >  2:165405/1‑148 (MQ=255)
                                                                                     acaAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCgggg                                                                                                                                   >  2:92918/1‑82 (MQ=255)
                                                                                       aaaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGCGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATACATACTCTCAGC‑AGCCCTGACACGTTCCCACGaaca                                                              >  8:131795/1‑148 (MQ=39)
                                                                                        aaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGaacaac                                                            <  4:227270/149‑1 (MQ=255)
                                                                                        aaaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGaacaac                                                            <  1:165405/149‑1 (MQ=255)
                                                                                           aaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCATGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAg                                                         >  7:130337/1‑149 (MQ=255)
                                                                                           aaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAg                                                         <  4:226860/149‑1 (MQ=255)
                                                                                                   aGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGc                                                 >  7:157428/1‑149 (MQ=255)
                                                                                                       gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACACTCCGC‑TGCCCTGCCACGATCCCAAGAACAACAAGGCCAACGCCATg                                             >  8:255720/1‑149 (MQ=255)
                                                                                                       gAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCGACATTCATACTCTCAGC‑TGCCCTGACACCATCCCAAGAACAACAAGGCCAAAGCCATg                                             >  2:206492/1‑149 (MQ=255)
                                                                                                             gCGGTAGGGCGTGTCTGAAGATCTGGTGTG‑CCTGGTAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGaccac                                       <  3:132366/149‑1 (MQ=255)
                                                                                                               ggTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATCGACCAccc                                     >  8:342849/1‑149 (MQ=255)
                                                                                                                gTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                     >  5:346189/1‑148 (MQ=255)
                                                                                                                        tgtCTGAAGATCTGGGGTG‑CCTGGGAGATCGTGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCATGCCCTGACTCGATCCCTAGAACAACAAGGCCAAAGCCATGGACCACCCCATAcccc                             <  1:107498/149‑1 (MQ=255)
                                                                                                                           cTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCAt                         >  8:112963/1‑149 (MQ=255)
                                                                                                                            tGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCTATGTTCATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                        <  7:255720/149‑1 (MQ=255)
                                                                                                                              aaGATCTGGGGTG‑CCTGGGAGATCGAGCAGCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAACCATGGACCACCCCATAGCCCGCATc                        <  4:126225/147‑1 (MQ=255)
                                                                                                                              aaGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATc                        <  6:346189/147‑1 (MQ=255)
                                                                                                                                 aTCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATCCCCCGCATCCTCAt                   >  6:113813/1‑149 (MQ=255)
                                                                                                                                  tCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtc                  >  8:364654/1‑149 (MQ=255)
                                                                                                                                      gggTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATTGACCACCCCATACCCCGCATCCTCAtcgt                <  7:342849/147‑1 (MQ=255)
                                                                                                                                        gggcccTGCGAGACCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtcg              >  4:53108/5‑147 (MQ=21)
                                                                                                                                          g‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAga          <  2:366669/149‑1 (MQ=255)
                                                                                                                                              tGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacga       >  2:346751/1‑149 (MQ=255)
                                                                                                                                               gggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac      >  5:347225/1‑149 (MQ=255)
                                                                                                                                                ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac      <  8:157428/148‑1 (MQ=255)
                                                                                                                                                ggAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAgacgac      <  8:31284/148‑1 (MQ=255)
                                                                                                                                                  agaCCGAGCGCCCCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAgc  <  3:53108/149‑1 (MQ=37)
                                                                                                                                                   gaTCGAGCACCGACCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCGGACACGATCCCTATCACAACATGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAgc  <  7:249862/149‑1 (MQ=255)
                                                                                                                                                    aTCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAgc  <  8:234599/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCTGTAGGCCTTGTGCCAAGTGGTTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTG‑CCTGGGAGATCGAGCACCGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGC‑TGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGC  >  NC_002947/1355876‑1356170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑