BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,380,722	G→T	6.0%	intergenic (‑110/+25)	PP_t67 ← / ← PP_t66	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,380,722	0	G	T	6.0%	319.6 / 8.3	100	intergenic (‑110/+25)	PP_t67/PP_t66	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base G (46/48); new base T (3/3); total (49/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-01

Only 100 of 101 total aligned reads displayed.

TTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTC  >  NC_002947/1380574‑1380861

ttATGAGCCCGACGTGCTACCAGGCTGCTACATCCCTCGTCAGTTGGGCGGATTCTACATTGTGTTTTTTGTATGTCATGCGCTAGGCTTTGATTTTTCGTCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACtgtg                                                                                                                                               <  1:265049/149‑1 (MQ=255)

   tGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGtt                                                                                                                                             <  5:119575/148‑1 (MQ=255)

     aGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGTCCACTGTGTTaa                                                                                                                                           >  3:36528/1‑148 (MQ=255)

        ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAg                                                                                                                                        <  5:357212/148‑1 (MQ=255)

         cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                      <  4:266035/149‑1 (MQ=255)

         cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                      <  2:32263/149‑1 (MQ=255)

         cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                      <  2:26191/149‑1 (MQ=255)

         cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                      <  1:306702/149‑1 (MQ=255)

            cgtgCTACCAGGCGTCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCATCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGcc                                                                                                                                   <  4:36528/146‑1 (MQ=255)

            cgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGcc                                                                                                                                   <  6:32104/149‑1 (MQ=255)

              aGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtt                                                                                                                                 <  1:25847/149‑1 (MQ=255)

                cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtttt                                                                                                                               <  4:349955/149‑1 (MQ=255)

                  aCCTGGCTGGACCTTCCGGCTTCCGTGGGGGGGTTTCTACTGTGTGTGGTTTGTATGTCAAGCGCTTGGCTTTTATTTTTCTACGTTTTTTGGCTATTGCTTATCAGCAGGCAAATAAAACGCCCACTGTGTAAACAGGGGCCTTTTc                                                                                                                              <  2:90564/148‑1 (MQ=255)

                   ccAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTc                                                                                                                              <  7:72303/147‑1 (MQ=255)

                   ccAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCtt                                                                                                                            <  5:203019/149‑1 (MQ=255)

                      ggCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑aa                                                                                                                        >  6:82350/1‑149 (MQ=255)

                      ggCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑aa                                                                                                                        <  8:46396/149‑1 (MQ=255)

                           ctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCt                                                                                                                     <  5:126238/147‑1 (MQ=255)

                             ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCt                                                                                                                             >  6:290088/1‑138 (MQ=255)

                             ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCt                                                                                                                             <  5:290088/138‑1 (MQ=255)

                             ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGtt                                                                                                                 >  4:190939/1‑149 (MQ=255)

                              cATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTgg                                                                                                                   <  7:94278/146‑1 (MQ=255)

                              cATCCCGCGTCAGTGCGGCTGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGCCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGtt                                                                                                                 <  3:48009/148‑1 (MQ=255)

                                tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCg                                                                                                              >  3:313565/1‑149 (MQ=255)

                                tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCg                                                                                                              >  3:2415/1‑149 (MQ=255)

                                 cccGCGTCAGTCGGGCGGATTCTACAGTGTCTGGCCTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAACGAAAAAGGCCACTGTGTTAACTGTGGCCTTTTCTTG‑AATCTGGTTGCgg                                                                                                             <  8:346659/149‑1 (MQ=18)

                                  ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCgg                                                                                                             >  6:187856/1‑148 (MQ=255)

                                  ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTAATCAGCAGGCAAAGAAAAAGGCCACTCTGTTAACAGTGGCCTATTCTTG‑AATCTGGATGCggg                                                                                                            >  2:199970/1‑149 (MQ=255)

                                   cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCCtttttcttt                                                                                                                          <  2:262428/135‑6 (MQ=18)

                                   cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCCtttttcttt                                                                                                                          >  1:262428/1‑130 (MQ=18)

                                   cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCgg                                                                                                             >  8:25823/1‑147 (MQ=255)

                                          gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGAt                                                                                                    <  2:28194/149‑1 (MQ=255)

                                          gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGAt                                                                                                    >  5:197515/1‑149 (MQ=255)

                                          gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCACGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGAt                                                                                                    >  6:639/1‑149 (MQ=255)

                                             gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCACGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTg                                                                                                 <  5:639/149‑1 (MQ=255)

                                             gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTAATTATTAGACGTTTTTTAGAAAATGATTATAATCACTCAAAGAAAAAGTCAACTTTGTTAACAGTGGACTTTTCTTT‑AATCTGGTTGCGGGAGCCGGAttt                                                                                                  >  4:198218/1‑148 (MQ=17)

                                                cGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                              <  4:2415/149‑1 (MQ=255)

                                                cGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                              >  2:284454/1‑149 (MQ=255)

                                                cGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCAATGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                              >  1:238203/1‑149 (MQ=255)

                                                       tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTTTCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACATTTGACTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCgccgac                                                                                       >  3:319836/1‑149 (MQ=18)

                                                       tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGc                                                                                                                                    <  8:361474/105‑1 (MQ=21)

                                                       tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                       >  3:213455/1‑149 (MQ=255)

                                                         cAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCt                                                                                     >  5:241947/1‑149 (MQ=255)

                                                              tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                 <  6:241947/148‑1 (MQ=255)

                                                              tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                 <  4:313565/148‑1 (MQ=255)

                                                              tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                 <  3:190939/148‑1 (MQ=255)

                                                               gtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGt                                                                               >  3:338555/1‑149 (MQ=255)

                                                                   tttGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGCAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                            <  1:284454/149‑1 (MQ=255)

                                                                       tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGt                                                                               >  4:14666/1‑141 (MQ=255)

                                                                       tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGt                                                                               <  3:14666/141‑1 (MQ=255)

                                                                       tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGc                                                                       <  5:82350/148‑1 (MQ=255)

                                                                       tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTAAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGcc                                                                      <  5:187856/149‑1 (MQ=255)

                                                                             aaGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCcgacg                                                                 <  6:197515/148‑1 (MQ=255)

                                                                             aaGCGCTAGGCTTTCATTATTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCcgacg                                                                 <  1:156276/148‑1 (MQ=255)

                                                                             aaGCGCTAGGCGTTGATTTTTCGACTTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGGCACTGTGTTAACAGTGGCCTTTTGTTG‑AATCTGGTTGCGGTAGCCTGATTTGAACCGACGACCTTCGGGTTATG‑AGCCcgacga                                                                <  1:199970/149‑1 (MQ=17)

                                                                              aGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAg                                                               >  4:224447/1‑149 (MQ=255)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGTCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCACGACGAGCTACCAGgct                                                     >  5:55917/1‑149 (MQ=255)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                 <  1:194955/122‑1 (MQ=255)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                 >  2:194955/1‑122 (MQ=255)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                           <  1:326127/128‑1 (MQ=255)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                           >  2:326127/1‑128 (MQ=255)

                                                                                        tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGgct                                                     >  5:306993/1‑149 (MQ=255)

                                                                                          tGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGgctgc                                                   >  2:335821/1‑149 (MQ=255)

                                                                                           gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAgg                                                       <  4:337737/144‑1 (MQ=255)

                                                                                           gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAgg                                                       >  3:337737/1‑144 (MQ=255)

                                                                                             tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑a                                                                         >  3:369099/1‑124 (MQ=255)

                                                                                             tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑a                                                                         <  4:369099/124‑1 (MQ=255)

                                                                                                  cGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATcc                                            <  4:213455/148‑1 (MQ=255)

                                                                                                   gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCcg                                          >  7:40847/1‑149 (MQ=255)

                                                                                                   gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGAGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCACGACGAGCTACCAGGCTGCTCCATCCcg                                          >  3:237769/1‑149 (MQ=255)

                                                                                                      gTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGt                                       <  7:25823/149‑1 (MQ=255)

                                                                                                             cgcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTGTCTTG‑AATCTGGTTCCGGGAGCCGGAGTTGAACCGAAGACCTTCGGGTTATG‑CGCCCGACGAGCTACCAGGCTGCTCCGTCCCGCGTCCGTggg                                >  5:387182/1‑149 (MQ=17)

                                                                                                              gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                               >  2:379331/1‑149 (MQ=255)

                                                                                                                  aTGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                           <  6:306993/149‑1 (MQ=255)

                                                                                                                  aTGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                           <  3:224447/149‑1 (MQ=255)

                                                                                                                    gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                       <  2:166084/88‑1 (MQ=255)

                                                                                                                    gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                       >  1:166084/1‑88 (MQ=255)

                                                                                                                    gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTc                                                                                   >  6:24684/1‑92 (MQ=255)

                                                                                                                    gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTc                                                                                   <  5:24684/92‑1 (MQ=255)

                                                                                                                      ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTc                        >  7:366916/1‑148 (MQ=255)

                                                                                                                           agcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAAGCCCGACGAGCTACCAGGCTGCTCCAt                                              <  1:223660/122‑1 (MQ=255)

                                                                                                                           agcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCAt                                              >  2:223660/1‑121 (MQ=39)

                                                                                                                            gcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCg                 >  8:39820/1‑149 (MQ=255)

                                                                                                                              agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGTATTTGAACCGACGACCTTCGGGTTTTG‑AGCCCGACGATCTACCAGGCTGCTACATCCCGCGTCAGTGGGGCGGATTCTACAGCgtg               <  2:238203/149‑1 (MQ=255)

                                                                                                                              agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCgtg               >  7:27595/1‑149 (MQ=255)

                                                                                                                                gCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCgtg               >  3:93543/1‑147 (MQ=255)

                                                                                                                                   aaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGtt          >  3:85171/1‑149 (MQ=255)

                                                                                                                                     gAAAATGGCCACTGTGTTAACTGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTta        <  4:237769/149‑1 (MQ=255)

                                                                                                                                      aaaaaGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTta        >  3:323237/1‑148 (MQ=255)

                                                                                                                                        aaaGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCACGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTatat     >  7:108034/1‑149 (MQ=255)

                                                                                                                                         aaGGCCACTGTTAACACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATg    >  5:128352/1‑149 (MQ=255)

                                                                                                                                         aaGGCCACTGTGTTAACAGTGGCCTTTTGTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATg    <  4:338555/149‑1 (MQ=255)

                                                                                                                                         aaGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGc                  >  8:127160/1‑135 (MQ=255)

                                                                                                                                         aaGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGc                  <  7:127160/135‑1 (MQ=255)

                                                                                                                                           ggCCACTGTTAACACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGt   >  2:197149/1‑148 (MQ=255)

                                                                                                                                            gCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGCCGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTc  >  7:139409/1‑148 (MQ=255)

                                                                                                                                              cACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGc                                                              <  3:85847/86‑1 (MQ=38)

                                                                                                                                              cACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGc                                                              >  4:85847/1‑86 (MQ=38)

                                                                                                                                               aCTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtat       >  1:232789/1‑140 (MQ=255)

                                                                                                                                               aCTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtat       <  2:232789/140‑1 (MQ=255)

TTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTC  >  NC_002947/1380574‑1380861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`