BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,380,723	T→A	6.0%	intergenic (‑111/+24)	PP_t67 ← / ← PP_t66	tRNA‑Met/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,380,723	0	T	A	6.0%	290.4 / 5.6	99	intergenic (‑111/+24)	PP_t67/PP_t66	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand): ref base T (46/47); new base A (3/3); total (49/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 101 total aligned reads displayed.

TGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGG  >  NC_002947/1380577‑1380871

tGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGtt                                                                                                                                                       <  5:119575/148‑1 (MQ=255)

  aGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGTCCACTGTGTTaa                                                                                                                                                     >  3:36528/1‑148 (MQ=255)

     ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAg                                                                                                                                                  <  5:357212/148‑1 (MQ=255)

      cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                                <  4:266035/149‑1 (MQ=255)

      cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                                <  1:306702/149‑1 (MQ=255)

      cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                                <  2:32263/149‑1 (MQ=255)

      cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                                <  2:26191/149‑1 (MQ=255)

         cgtgCTACCAGGCGTCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCATCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGcc                                                                                                                                             <  4:36528/146‑1 (MQ=255)

         cgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGcc                                                                                                                                             <  6:32104/149‑1 (MQ=255)

           aGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtt                                                                                                                                           <  1:25847/149‑1 (MQ=255)

             cTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCtttt                                                                                                                                         <  4:349955/149‑1 (MQ=255)

               aCCTGGCTGGACCTTCCGGCTTCCGTGGGGGGGTTTCTACTGTGTGTGGTTTGTATGTCAAGCGCTTGGCTTTTATTTTTCTACGTTTTTTGGCTATTGCTTATCAGCAGGCAAATAAAACGCCCACTGTGTAAACAGGGGCCTTTTc                                                                                                                                        <  2:90564/148‑1 (MQ=255)

                ccAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTc                                                                                                                                        <  7:72303/147‑1 (MQ=255)

                ccAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCtt                                                                                                                                      <  5:203019/149‑1 (MQ=255)

                   ggCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑aa                                                                                                                                  >  6:82350/1‑149 (MQ=255)

                   ggCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑aa                                                                                                                                  <  8:46396/149‑1 (MQ=255)

                        ctCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCt                                                                                                                               <  5:126238/147‑1 (MQ=255)

                          ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCt                                                                                                                                       <  5:290088/138‑1 (MQ=255)

                          ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCt                                                                                                                                       >  6:290088/1‑138 (MQ=255)

                          ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGtt                                                                                                                           >  4:190939/1‑149 (MQ=255)

                           cATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTgg                                                                                                                             <  7:94278/146‑1 (MQ=255)

                           cATCCCGCGTCAGTGCGGCTGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGCCGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGtt                                                                                                                           <  3:48009/148‑1 (MQ=255)

                             tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCg                                                                                                                        >  3:313565/1‑149 (MQ=255)

                             tCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCg                                                                                                                        >  3:2415/1‑149 (MQ=255)

                              cccGCGTCAGTCGGGCGGATTCTACAGTGTCTGGCCTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAACGAAAAAGGCCACTGTGTTAACTGTGGCCTTTTCTTG‑AATCTGGTTGCgg                                                                                                                       <  8:346659/149‑1 (MQ=18)

                               ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCgg                                                                                                                       >  6:187856/1‑148 (MQ=255)

                               ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTAATCAGCAGGCAAAGAAAAAGGCCACTCTGTTAACAGTGGCCTATTCTTG‑AATCTGGATGCggg                                                                                                                      >  2:199970/1‑149 (MQ=255)

                                cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCCtttttcttt                                                                                                                                    <  2:262428/135‑6 (MQ=18)

                                cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGCCtttttcttt                                                                                                                                    >  1:262428/1‑130 (MQ=18)

                                cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCgg                                                                                                                       >  8:25823/1‑147 (MQ=255)

                                       gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGAt                                                                                                              <  2:28194/149‑1 (MQ=255)

                                       gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGAt                                                                                                              >  5:197515/1‑149 (MQ=255)

                                       gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCACGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGAt                                                                                                              >  6:639/1‑149 (MQ=255)

                                          gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCACGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTg                                                                                                           <  5:639/149‑1 (MQ=255)

                                          gggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTAATTATTAGACGTTTTTTAGAAAATGATTATAATCACTCAAAGAAAAAGTCAACTTTGTTAACAGTGGACTTTTCTTT‑AATCTGGTTGCGGGAGCCGGAttt                                                                                                            >  4:198218/1‑148 (MQ=17)

                                             cGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                        <  4:2415/149‑1 (MQ=255)

                                             cGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                        >  2:284454/1‑149 (MQ=255)

                                             cGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCAATGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAAc                                                                                                        >  1:238203/1‑149 (MQ=255)

                                                    tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTTTCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACATTTGACTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCgccgac                                                                                                 >  3:319836/1‑149 (MQ=18)

                                                    tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGc                                                                                                                                              <  8:361474/105‑1 (MQ=21)

                                                    tACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                                 >  3:213455/1‑149 (MQ=255)

                                                      cAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCt                                                                                               >  5:241947/1‑149 (MQ=255)

                                                           tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                           <  4:313565/148‑1 (MQ=255)

                                                           tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                           <  6:241947/148‑1 (MQ=255)

                                                           tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                           <  3:190939/148‑1 (MQ=255)

                                                            gtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGt                                                                                         >  3:338555/1‑149 (MQ=255)

                                                                tttGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTGCAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                      <  1:284454/149‑1 (MQ=255)

                                                                    tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGt                                                                                         >  4:14666/1‑141 (MQ=255)

                                                                    tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGt                                                                                         <  3:14666/141‑1 (MQ=255)

                                                                    tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGc                                                                                 <  5:82350/148‑1 (MQ=255)

                                                                    tATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTAAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGcc                                                                                <  5:187856/149‑1 (MQ=255)

                                                                          aaGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCcgacg                                                                           <  6:197515/148‑1 (MQ=255)

                                                                          aaGCGCTAGGCTTTCATTATTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCcgacg                                                                           <  1:156276/148‑1 (MQ=255)

                                                                          aaGCGCTAGGCGTTGATTTTTCGACTTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGGCACTGTGTTAACAGTGGCCTTTTGTTG‑AATCTGGTTGCGGTAGCCTGATTTGAACCGACGACCTTCGGGTTATG‑AGCCcgacga                                                                          <  1:199970/149‑1 (MQ=17)

                                                                           aGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAg                                                                         >  4:224447/1‑149 (MQ=255)

                                                                                     tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGTCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCACGACGAGCTACCAGgct                                                               >  5:55917/1‑149 (MQ=255)

                                                                                     tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                           <  1:194955/122‑1 (MQ=255)

                                                                                     tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                           >  2:194955/1‑122 (MQ=255)

                                                                                     tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                                     <  1:326127/128‑1 (MQ=255)

                                                                                     tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATg                                                                                     >  2:326127/1‑128 (MQ=255)

                                                                                     tttGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGgct                                                               >  5:306993/1‑149 (MQ=255)

                                                                                       tGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGgctgc                                                             >  2:335821/1‑149 (MQ=255)

                                                                                        gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAgg                                                                 <  4:337737/144‑1 (MQ=255)

                                                                                        gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAgg                                                                 >  3:337737/1‑144 (MQ=255)

                                                                                          tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑a                                                                                   >  3:369099/1‑124 (MQ=255)

                                                                                          tttttCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑a                                                                                   <  4:369099/124‑1 (MQ=255)

                                                                                               cGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATcc                                                      <  4:213455/148‑1 (MQ=255)

                                                                                                gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCcg                                                    >  7:40847/1‑149 (MQ=255)

                                                                                                gACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGAGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCACGACGAGCTACCAGGCTGCTCCATCCcg                                                    >  3:237769/1‑149 (MQ=255)

                                                                                                   gTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGt                                                 <  7:25823/149‑1 (MQ=255)

                                                                                                          cgcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTGTCTTG‑AATCTGGTTCCGGGAGCCGGAGTTGAACCGAAGACCTTCGGGTTATG‑CGCCCGACGAGCTACCAGGCTGCTCCGTCCCGCGTCCGTggg                                          >  5:387182/1‑149 (MQ=17)

                                                                                                           gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                                         >  2:379331/1‑149 (MQ=255)

                                                                                                               aTGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                                     <  6:306993/149‑1 (MQ=255)

                                                                                                               aTGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                                     <  3:224447/149‑1 (MQ=255)

                                                                                                                 gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                                 >  1:166084/1‑88 (MQ=255)

                                                                                                                 gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCgacgac                                                                                                 <  2:166084/88‑1 (MQ=255)

                                                                                                                 gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTc                                                                                             <  5:24684/92‑1 (MQ=255)

                                                                                                                 gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTc                                                                                             >  6:24684/1‑92 (MQ=255)

                                                                                                                   ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTc                                  >  7:366916/1‑148 (MQ=255)

                                                                                                                        agcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAAGCCCGACGAGCTACCAGGCTGCTCCAt                                                        <  1:223660/122‑1 (MQ=255)

                                                                                                                        agcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCAt                                                        >  2:223660/1‑121 (MQ=39)

                                                                                                                         gcagGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCg                           >  8:39820/1‑149 (MQ=255)

                                                                                                                           agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGTATTTGAACCGACGACCTTCGGGTTTTG‑AGCCCGACGATCTACCAGGCTGCTACATCCCGCGTCAGTGGGGCGGATTCTACAGCgtg                         <  2:238203/149‑1 (MQ=255)

                                                                                                                           agGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCgtg                         >  7:27595/1‑149 (MQ=255)

                                                                                                                             gCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCgtg                         >  3:93543/1‑147 (MQ=255)

                                                                                                                                aaGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGtt                    >  3:85171/1‑149 (MQ=255)

                                                                                                                                  gAAAATGGCCACTGTGTTAACTGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTta                  <  4:237769/149‑1 (MQ=255)

                                                                                                                                   aaaaaGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTta                  >  3:323237/1‑148 (MQ=255)

                                                                                                                                     aaaGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCACGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTatat               >  7:108034/1‑149 (MQ=255)

                                                                                                                                      aaGGCCACTGTTAACACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATg              >  5:128352/1‑149 (MQ=255)

                                                                                                                                      aaGGCCACTGTGTTAACAGTGGCCTTTTGTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATg              <  4:338555/149‑1 (MQ=255)

                                                                                                                                      aaGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGc                            >  8:127160/1‑135 (MQ=255)

                                                                                                                                      aaGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGc                            <  7:127160/135‑1 (MQ=255)

                                                                                                                                        ggCCACTGTTAACACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGt             >  2:197149/1‑148 (MQ=255)

                                                                                                                                         gCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGCCGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTc            >  7:139409/1‑148 (MQ=255)

                                                                                                                                           cACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGc                                                                        <  3:85847/86‑1 (MQ=38)

                                                                                                                                           cACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGc                                                                        >  4:85847/1‑86 (MQ=38)

                                                                                                                                            aCTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtat                 >  1:232789/1‑140 (MQ=255)

                                                                                                                                            aCTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtat                 <  2:232789/140‑1 (MQ=255)

                                                                                                                                                  ttAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAgg  >  2:388954/1‑149 (MQ=255)

TGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCCTTTTCTTG‑AATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATG‑AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTAGG  >  NC_002947/1380577‑1380871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`