Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,445,047 T→C 5.2% I224T (ATC→ACC)  PP_1263 → fusaric acid resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,445,0470TC 5.2% 233.3 / 2.1 77I224T (ATC→ACC) PP_1263fusaric acid resistance protein
Reads supporting (aligned to +/- strand):  ref base T (33/40);  new base C (2/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

GCCAGGCGCAGCTGGAACTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCGGGGGAAGGCGCGCAGTTGC  >  NC_002947/1444900‑1445193
                                                                                                                                                    |                                                                                                                                                  
gCCAGGCGCAGCTGGAACTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATc                                                                                                                                                   <  2:377854/149‑1 (MQ=255)
      cgcAGCTGGAACTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCACAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCACGCGTCGCCTGGATGCCGGT‑ATCGGCAcc                                                                                                                                             >  6:60616/1‑149 (MQ=255)
          gCTGGAACTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGACCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGGTGCCGAT‑ACCGGCATCCagg                                                                                                                                         <  1:85409/149‑3 (MQ=255)
               aaCTGCAGCAGAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATCTCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGaa                                                                                                                                    <  4:234844/149‑1 (MQ=255)
               aaCTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCTGGT‑ATCGGCACCCATTTCGaa                                                                                                                                    <  1:285981/149‑1 (MQ=255)
               aaCTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCg                                                                                                                                      >  5:207021/1‑147 (MQ=255)
               aaCTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGaa                                                                                                                                    <  6:207015/149‑1 (MQ=255)
               aaCTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGaa                                                                                                                                    <  6:236708/149‑1 (MQ=255)
               aaCTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGaa                                                                                                                                    <  7:364728/149‑1 (MQ=255)
               aaCTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGaa                                                                                                                                    <  2:266409/149‑1 (MQ=255)
                aCTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAAGTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGaa                                                                                                                                    <  8:131397/148‑1 (MQ=255)
                 cTGCAGAACAATGTGGTGCGTGCCTACACCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGt                                                                                                                                  <  5:227980/149‑1 (MQ=255)
                  tGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCTTAAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCACTGGCAAAGCGTCGCCTGGATTCCGGT‑ATCGGAACCCATTTAGAAGt                                                                                                                                  >  5:374756/1‑148 (MQ=255)
                   gCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGt                                                                                                                                  >  2:289457/1‑147 (MQ=255)
                    cAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAg                                                                                                                               >  3:258260/1‑149 (MQ=255)
                    cAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAg                                                                                                                               >  3:209548/1‑149 (MQ=255)
                     aGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGc                                                                                                                              >  7:329166/1‑149 (MQ=255)
                      gAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGc                                                                                                                              >  7:320248/1‑148 (MQ=255)
                       aaCAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAgt                                                                                                                              >  6:183074/1‑146 (MQ=255)
                       aaCAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAgg                                                                                                                              >  6:177318/1‑146 (MQ=255)
                              tggtgCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGaa                                                                                                                     <  2:393007/149‑1 (MQ=255)
                               ggtgCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAg                                                                                                                    >  4:195506/1‑149 (MQ=255)
                                   cgtgcCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGcgcc                                                                                                                <  7:289846/149‑1 (MQ=255)
                                      gcCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCAGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCt                                                                                                             >  4:117084/1‑149 (MQ=255)
                                         tACATCCAGCTGAGCCTGCATATTGCCCAGCGCGATATCGTAAACCCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGcc                                                                                                          >  2:361070/1‑149 (MQ=255)
                                           cATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCg                                                                                                         <  1:317117/148‑1 (MQ=255)
                                           cATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCg                                                                                                         <  3:363684/148‑1 (MQ=255)
                                             tCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGaa                                                                                                      <  8:200005/149‑1 (MQ=255)
                                                aGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGTAATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAAc                                                                                                    <  1:289457/149‑1 (MQ=255)
                                                     aGCCTGCATTTTTCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGATCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCTGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGTTGCCGGAAACCCAcc                                                                                               <  6:374756/148‑1 (MQ=255)
                                                        cTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAACCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGt                                                                                             >  7:176510/1‑147 (MQ=255)
                                                          gCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGc                                                                                         >  2:369984/1‑149 (MQ=255)
                                                            aTTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTc                                                                                       <  4:209548/149‑1 (MQ=255)
                                                             ttttGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTc                                                                                       >  3:282106/1‑148 (MQ=255)
                                                                  cccAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGc                                                                                 <  4:258260/149‑1 (MQ=255)
                                                                       cgcgATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTcga                                                                            <  4:266850/149‑1 (MQ=255)
                                                                           atatCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACgaa                                                                        <  5:367286/149‑1 (MQ=255)
                                                                              tCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACgaaga                                                                      <  6:197499/148‑1 (MQ=255)
                                                                                 tCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAAtt                                                                  <  5:299500/149‑1 (MQ=255)
                                                                                     ggCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGccc                                                              >  3:211917/1‑149 (MQ=255)
                                                                                     ggCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGccc                                                              <  6:88060/149‑1 (MQ=255)
                                                                                     ggCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGccc                                                              <  4:282106/149‑1 (MQ=255)
                                                                                     ggCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGccc                                                              <  1:393754/149‑1 (MQ=255)
                                                                                     ggCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGccc                                                              <  8:320248/149‑1 (MQ=255)
                                                                                        cGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGa                                                           >  1:106068/1‑149 (MQ=255)
                                                                                         gAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGAc                                                          <  3:195506/149‑1 (MQ=255)
                                                                                              ggAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGc                                                      >  2:110666/1‑148 (MQ=255)
                                                                                               gAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGc                                                      <  8:329166/147‑1 (MQ=255)
                                                                                                 aCAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAAcc                                                  <  1:110666/149‑1 (MQ=255)
                                                                                                  cagcagGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAACCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCa                                                 <  8:176510/149‑1 (MQ=255)
                                                                                                    gcagGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGc                                               >  3:311750/1‑149 (MQ=255)
                                                                                                           cAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCAGCAACCAGCTGGCCGc                                        >  6:103688/1‑149 (MQ=255)
                                                                                                             gATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCAc                                      <  1:369984/149‑1 (MQ=255)
                                                                                                                cctggccctggcCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTgg                                   >  8:169048/1‑149 (MQ=255)
                                                                                                                    gccctggccAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCgg                               <  7:169048/149‑1 (MQ=255)
                                                                                                                    gccctggccAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCg                                <  3:292943/148‑1 (MQ=255)
                                                                                                                       ctggccAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGc                              >  6:234435/1‑147 (MQ=255)
                                                                                                                       ctggccAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGc                              >  1:211516/1‑147 (MQ=255)
                                                                                                                        tggccAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCAGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTAGACGAAGAAATTGCCCTGACGCGCAACCAGCTGGCCGCACTGGCCGGCAAg                           >  2:163112/1‑149 (MQ=255)
                                                                                                                           ccAAGCGTCGCCTGGATCCCGGT‑ATCGGCAACCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGACGGAAACCCACCCTCAGCTCGACAGCATCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGc                        >  2:144005/1‑149 (MQ=255)
                                                                                                                            cAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCAcc                                                                                               <  2:76908/77‑1 (MQ=255)
                                                                                                                            cAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCAcc                                                                                               >  1:76908/1‑77 (MQ=255)
                                                                                                                            cAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGcc                       >  4:46969/1‑149 (MQ=255)
                                                                                                                            cAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGcc                       >  4:186880/1‑149 (MQ=255)
                                                                                                                              aGCGTCGCCTGGATGCCGGT‑CTCGGCACCCATTTCGAATTCAGCCAGGCCGAAGCGCCGCTGACGTAAACCCACCGTCAGCTCTACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCg                     <  8:300570/149‑1 (MQ=255)
                                                                                                                                 gTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCgggg                  <  5:234435/149‑1 (MQ=255)
                                                                                                                                   cGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCggggg                 >  1:135487/1‑148 (MQ=255)
                                                                                                                                    aaatGGGTGCCGAT‑ACCGGCATCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCggggg                 >  1:48989/4‑147 (MQ=255)
                                                                                                                                      cTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCGGGGGAAgg             >  3:279884/1‑149 (MQ=255)
                                                                                                                                      atGGGTGCCGAT‑ACCGGCATCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCGGGGGAAgg             >  8:274820/2‑149 (MQ=255)
                                                                                                                                             ccGAT‑ACCGGCATCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCGGGGGAAGGCGCGCa       <  7:274820/148‑1 (MQ=255)
                                                                                                                                              cGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCg                                         >  1:216830/1‑113 (MQ=255)
                                                                                                                                              cGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCg                                         <  2:216830/113‑1 (MQ=255)
                                                                                                                                              cGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCGGGGGAAGGCGCGCAGt     >  4:264159/1‑149 (MQ=255)
                                                                                                                                               ggT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTGAGCTCGACAGCCTCGACGAAGAAATTGCCCTGAGGCGCTTCCAGCTGGCCGCACTGGCCGGCAAGGGCCCGGGGGAAGGCGCGCAGtt    <  1:361070/149‑1 (MQ=255)
                                                                                                                                               ggT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCACCCAGCTGGCCGCACTGGCCGGCAAGGGCCCGGGGAAAGGCGCGCAGtt    <  2:340179/149‑1 (MQ=255)
                                                                                                                                                 t‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCGGGGGAAGGCGCGCAGTTGc  <  5:60616/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCCAGGCGCAGCTGGAACTGCAGAACAATGTGGTGCGTGCCTACATCCAGCTGAGCCTGCATTTTGCCCAGCGCGATATCGTCAAGGCCGAACTGGAACAGCAGGAGCAGATCCTGGCCCTGGCCAAGCGTCGCCTGGATGCCGGT‑ATCGGCACCCATTTCGAAGTCAGCCAGGCCGAAGCGCCGCTGCCGGAAACCCACCGTCAGCTCGACAGCCTCGACGAAGAAATTGCCCTGACCCGCAACCAGCTGGCCGCACTGGCCGGCAAGGGCCCGGGGGAAGGCGCGCAGTTGC  >  NC_002947/1444900‑1445193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑