Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,449,139	T→C	6.4%	*287R (TGA→CGA)	PP_1266 →	fusaric acid resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,449,139	0	T	C	6.4%	177.9 / 2.6	62	*287R (TGA→CGA)	PP_1266	fusaric acid resistance protein
Reads supporting (aligned to +/- strand):  ref base T (22/36);  new base C (2/2);  total (24/38)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-01

CGAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCTTT  >  NC_002947/1448991‑1449283                                                                                                                                                     |                                                                                                                                                 cgAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGtt                                                                                                                                                  <  4:90106/149‑1 (MQ=255) cgAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGtt                                                                                                                                                  <  4:84799/149‑1 (MQ=255)      tGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCt                                                                                                                                             >  6:62822/1‑149 (MQ=255)        aTCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTc                                                                                                                                           >  2:261782/1‑149 (MQ=255)           gCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTCGCGAAGGTCAACCTTcgc                                                                                                                                         <  2:46317/148‑1 (MQ=255)            ccGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGa                                                                                                                                       >  2:370234/1‑149 (MQ=255)            ccGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGa                                                                                                                                       <  2:247022/149‑1 (MQ=255)             cGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGAc                                                                                                                                      >  5:135171/1‑149 (MQ=255)                cGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTCAACCTTcgccaggct                                                                                                                                   <  6:387070/149‑7 (MQ=255)                   gAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGG‑TTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCg                                                                                                                               >  4:15192/1‑149 (MQ=255)                     aCCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCgg                                                                                                                              >  4:55051/1‑149 (MQ=255)                      cccGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGc                                                                                                                             >  8:168122/1‑149 (MQ=255)                      cccGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGc                                                                                                                             <  7:76341/149‑1 (MQ=255)                            cTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCg                                                                                                                       >  2:226852/1‑149 (MQ=255)                            cTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCg                                                                                                                       >  4:89450/1‑149 (MQ=255)                             ttCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCg                                                                                                                       >  6:149695/1‑148 (MQ=255)                              tCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGca                                                                                                                     >  5:383502/1‑149 (MQ=255)                               cAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGca                                                                                                                     <  1:204051/148‑1 (MQ=255)                                 aCTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGcatca                                                                                                                  <  1:226852/149‑1 (MQ=255)                                      gTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCtgt                                                                                                             <  5:62822/149‑1 (MQ=255)                                      gTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCtgt                                                                                                             <  5:208512/149‑1 (MQ=255)                                      gTGCGGCTGGCACAGCGGGGGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCtgt                                                                                                             <  3:103698/149‑1 (MQ=255)                                        ggtgCTGGCTCAGCGGGGGCCGGGGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTgg                                                                                                           <  8:188812/146‑1 (MQ=255)                                         cGGCTGGCACAGCGGGGGCCGGGGCGCATTCACATCGATGAAGAGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTgg                                                                                                           <  2:143710/148‑1 (MQ=255)                                           gCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAg                                                                                                        <  5:94876/149‑1 (MQ=255)                                           gCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAg                                                                                                        >  2:148842/1‑149 (MQ=255)                                               gCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCct                                                                                                    >  8:31555/1‑149 (MQ=255)                                                         tGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGatgcat                                                                                          <  8:33470/149‑1 (MQ=255)                                                         tGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGatgcat                                                                                          <  3:55051/149‑1 (MQ=255)                                                           ccGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGatgcatgc                                                                                        <  6:383502/149‑1 (MQ=255)                                                                 cgcATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTAtt                                                                                   <  4:236811/148‑1 (MQ=255)                                                                   cATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGc                                                                                <  3:265938/149‑1 (MQ=255)                                                                   cATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGc                                                                                <  2:122189/149‑1 (MQ=255)                                                                   cATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGc                                                                                <  5:161725/149‑1 (MQ=255)                                                                    aTTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGc                                                                                >  7:213975/1‑148 (MQ=255)                                                                     ttCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCa                                                                              <  1:370234/149‑1 (MQ=255)                                                                        acaTCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTc                                                                           <  5:149695/149‑1 (MQ=255)                                                                         caTCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCg                                                                          <  4:301863/149‑1 (MQ=255)                                                                             gATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCt                                                                       <  6:194468/148‑1 (MQ=255)                                                                             gATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCt                                                                       <  6:189032/148‑1 (MQ=255)                                                                                   gTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGACGGCTCAATCTg                                                                >  2:357667/1‑149 (MQ=255)                                                                                      ccGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCgg                                                             >  6:126584/1‑149 (MQ=255)                                                                                                   tGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTgca                                                 <  7:31555/148‑1 (MQ=255)                                                                                                           gcggGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGt                                        <  1:20521/149‑1 (MQ=255)                                                                                                           gcggGGACGACGGGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGTCCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGt                                        <  4:293404/149‑1 (MQ=255)                                                                                                            cggGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTc                                       <  3:89450/149‑1 (MQ=255)                                                                                                            cggGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTc                                       <  8:213975/149‑1 (MQ=255)                                                                                                             ggggACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTcc                                      <  5:203211/149‑1 (MQ=255)                                                                                                                      gtgtACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGATGATGCATGCGTATTAGCAAGTCGGCTCAATCTCCGGCAGCCTCTTGCAGCAACTGGTCCAAAtcctcc                             >  1:395236/1‑149 (MQ=255)                                                                                                                         tACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCgg                           <  6:135171/148‑1 (MQ=255)                                                                                                                         tACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGt                          >  1:272199/1‑149 (MQ=255)                                                                                                                          aCGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTg                         >  6:52240/1‑149 (MQ=255)                                                                                                                           cGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTgg                        <  1:148842/149‑1 (MQ=255)                                                                                                                               ggtAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTg                    <  3:15192/149‑1 (MQ=255)                                                                                                                                   gTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTgg                                         >  4:280082/1‑124 (MQ=255)                                                                                                                                   gTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTgg                                         <  3:280082/124‑1 (MQ=255)                                                                                                                                      aaGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTAt             >  8:239186/1‑149 (MQ=255)                                                                                                                                      aaGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCACCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTAt             <  7:168122/149‑1 (MQ=255)                                                                                                                                        tggtcgCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATg            >  5:378893/6‑148 (MQ=255)                                                                                                                                           tGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGGATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCGTCGTGCAGCAACTGGTGCCATGCGACCTGTGGGTTGGCGGTATga           >  8:312006/1‑145 (MQ=255)                                                                                                                                             gCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACAt      <  5:126584/149‑1 (MQ=255)                                                                                                                                             gCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACAt      >  2:8315/1‑149 (MQ=255)                                                                                                                                                 aGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCTCAATCGTCCGGTGGGTTGCCGCTATGCAACATCttt  <  1:261782/149‑1 (MQ=255)                                                                                                                                                 aGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCttt  <  7:312006/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTGGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATCTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCTTT  >  NC_002947/1448991‑1449283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑