Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 155,543 G→T 6.8% intergenic (‑248/+35) yjbB ← / ← ygdQ Na+/Pi‑cotransporter/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947155,5430GT 6.8% 233.3 / 4.3 73intergenic (‑248/+35)yjbB/ygdQNa+/Pi‑cotransporter/transport protein
Reads supporting (aligned to +/- strand):  ref base G (34/34);  new base T (3/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

CTGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGC  >  NC_002947/155398‑155683
                                                                                                                                                 |                                                                                                                                            
cTGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCg                                                                                                                                            >  1:352815/1‑148 (MQ=255)
   tCGCCAGCTTCGCATTGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGaa                                                                                                                                          <  3:277377/147‑1 (MQ=255)
   tCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAgg                                                                                                                                        <  7:188546/149‑1 (MQ=255)
     gCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGc                                                                                                                                      >  1:131436/1‑149 (MQ=255)
      ccAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGcc                                                                                                                                     >  1:243187/1‑149 (MQ=255)
        aGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGCCCt                                                                                                                                   <  3:293171/149‑1 (MQ=255)
          cTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttg                                                                                                                                 >  3:92732/1‑149 (MQ=255)
             cGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttgtt                                                                                                                               <  7:17417/148‑1 (MQ=255)
               cATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTtgtg                                                                                                                            <  4:12289/149‑1 (MQ=255)
               cATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTtgtg                                                                                                                            <  2:104980/149‑1 (MQ=255)
                aTGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgc                                                                                                                           <  1:115728/149‑1 (MQ=255)
                 tGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgct                                                                                                                          >  6:224321/1‑149 (MQ=255)
                  ggCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgctg                                                                                                                         >  2:66733/1‑149 (MQ=255)
                         atttatttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTcc                                                                                                                  <  2:352815/149‑1 (MQ=255)
                          tttatttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCt                                                                                                                 <  8:319276/149‑1 (MQ=255)
                            tatttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCtt                                                                                                                <  4:92732/148‑1 (MQ=255)
                              tttGAAAGAGATCTTGAAACGAATGACTGCCCGTGGTGGCTGCTTCGCGTGCAAGCCACCACAGTGACAACTCGCAGGAAAAAACGCACATAAATAAGGCATTAAATAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACtt                                                                                                                    <  6:51151/142‑1 (MQ=255)
                              tttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACtt                                                                                                                    >  5:51151/1‑142 (MQ=255)
                                  aaaCAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCtt                                                                                                          <  7:309315/148‑1 (MQ=255)
                                   aaCAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTAt                                                                                                        <  6:100603/149‑1 (MQ=255)
                                   aaCAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTAt                                                                                                        <  7:160040/149‑1 (MQ=255)
                                   aaCAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTAt                                                                                                        <  2:243187/149‑1 (MQ=255)
                                   aaCAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCATGTTGTGCTGCACTTCCTTGGCCTTAt                                                                                                        >  5:388107/1‑149 (MQ=255)
                                      aGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGa                                                                                                     >  2:136157/1‑149 (MQ=255)
                                          cTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCg                                                                                                 <  5:306551/149‑1 (MQ=255)
                                               aaCGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATg                                                                                            >  2:374562/1‑149 (MQ=255)
                                                 cGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTc                                                                                          >  4:383607/1‑149 (MQ=255)
                                                    aTGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCtt                                                                                       <  3:383607/149‑1 (MQ=255)
                                                        cTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTtgtg                                                                                                                            >  6:372805/1‑108 (MQ=255)
                                                        cTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTtgtg                                                                                                                            <  5:372805/108‑1 (MQ=255)
                                                           cccGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCa                                                                                  <  5:224321/147‑1 (MQ=255)
                                                                 ggggCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCAc                                                                           <  3:278361/148‑1 (MQ=255)
                                                                 ggggCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCAc                                                                           <  2:131436/148‑1 (MQ=255)
                                                                  gggCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCg                                                                         <  1:374562/149‑1 (MQ=255)
                                                                      tgctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGtt                                                                      >  5:233961/1‑148 (MQ=255)
                                                                        ctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAATGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCAt                                                                   <  5:376342/149‑1 (MQ=255)
                                                                        ctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCa                                                                    <  7:281924/148‑1 (MQ=255)
                                                                           cgcgGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGAt                                                                >  2:309334/1‑149 (MQ=255)
                                                                            gcgGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATc                                                               >  4:370220/1‑149 (MQ=255)
                                                                                     ccaccaCAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCtt                                                            <  4:67460/143‑1 (MQ=255)
                                                                                     ccaccaCAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCtt                                                            >  3:67460/1‑143 (MQ=255)
                                                                                     ccaccaCAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATc                                                      >  4:151548/1‑149 (MQ=255)
                                                                                     ccaccaCAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATc                                                      >  8:116824/1‑149 (MQ=255)
                                                                                        ccacAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTc                                                                     <  1:42673/131‑1 (MQ=255)
                                                                                        ccacAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTc                                                                     >  2:42673/1‑131 (MQ=255)
                                                                                        ccacAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCtt                                                   >  4:112600/1‑149 (MQ=255)
                                                                                                          ggAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATGCTTGCCATCCTTGCGGGCCATGGCcttctc                                 >  3:219555‑M1/1‑143 (MQ=255)
                                                                                                           gAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGG                                              >  8:327433‑M1/1‑135 (MQ=255)
                                                                                                           gAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGG                                              <  7:327433‑M1/135‑1 (MQ=255)
                                                                                                            aaaaaaCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCACGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCa                               >  5:131585/1‑149 (MQ=255)
                                                                                                                 aCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCgg                          <  3:151548/149‑1 (MQ=255)
                                                                                                                   gCACCGAACAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCGTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGAt                        <  6:388107/149‑1 (MQ=255)
                                                                                                                    cacaGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATg                       <  5:264059/149‑1 (MQ=255)
                                                                                                                     acaGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGt                      >  8:87656/1‑149 (MQ=255)
                                                                                                                     acaGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGt                      >  6:62192/1‑149 (MQ=255)
                                                                                                                     acaGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGt                      >  6:260465/1‑149 (MQ=255)
                                                                                                                     acaGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCACGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGt                      <  6:131585/149‑1 (MQ=255)
                                                                                                                        gAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGa                   >  7:275284/1‑149 (MQ=255)
                                                                                                                           aaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATgg                >  2:58177/1‑149 (MQ=255)
                                                                                                                            aaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGc               <  3:112600/149‑1 (MQ=255)
                                                                                                                             aaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCt              >  3:108629/1‑149 (MQ=255)
                                                                                                                               ggCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTc            <  6:233961/149‑1 (MQ=255)
                                                                                                                                  aTTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCAc         >  3:291161/1‑149 (MQ=255)
                                                                                                                                   ttAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCAcc        >  6:256222/1‑149 (MQ=255)
                                                                                                                                    tAAAAAGGGCCCTTCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGAAGTTTCACCGGTTCATGATCATTGCAATACTTGCGGGCCATGGCGGTGCGCATGCGGATGTTTATGGCTTCAACCg       >  8:373261/1‑149 (MQ=255)
                                                                                                                                    tAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCg       >  1:7642/1‑149 (MQ=255)
                                                                                                                                    caacaagggcCCTTCGCAGGGCCCTTTTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGG                                        <  3:274923‑M1/112‑1 (MQ=255)
                                                                                                                                    caacaagggcCCTTCGCAGGGCCCTTTTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGAGGGCCATGG                                        >  4:274923‑M1/5‑116 (MQ=255)
                                                                                                                                      aaaaGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGc      >  5:165872/1‑148 (MQ=255)
                                                                                                                                       caaGGGCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGc      >  8:135700/2‑147 (MQ=255)
                                                                                                                                         aGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGc  <  1:66733/149‑1 (MQ=255)
                                                                                                                                                tGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTg                                                           <  1:291676/85‑1 (MQ=255)
                                                                                                                                                tGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTg                                                           >  2:291676/1‑85 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
CTGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGC  >  NC_002947/155398‑155683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑