Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,501,254 G→A 9.5% A152V (GCC→GTC)  PP_1313 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,501,2540GA 9.5% 114.3 / 2.5 43A152V (GCC→GTC) PP_1313AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (17/21);  new base A (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

TCAGGTCCTGGTGCAGCTTCTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTGAAGAAGCGCCAGTAGGTGGTCGCTTCCTTGCCATCGAGCAGGCCGG  >  NC_002947/1501108‑1501398
                                                                                                                                                  |                                                                                                                                                
tCAGGTCCTGGTGCAGCTTCTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCt                                                                                                                                                <  5:389734/149‑1 (MQ=255)
tCAGGTCCTGGTGCAGCTTCTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCt                                                                                                                                                >  4:355472/1‑149 (MQ=255)
tCAGGTCCTGGTGCAGCTTCTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCt                                                                                                                                                >  3:367964/1‑149 (MQ=255)
     tCCTGGTGCAGCTTCTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGACGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGt                                                                                                                                           >  5:188219/1‑149 (MQ=255)
      ccTGGTGCAGCTTCTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGtt                                                                                                                                          <  5:6055/149‑1 (MQ=255)
          tggCAGCTCGTGCCCGCCCATGCCCATGCGCGCCGGTGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGTCTGAAGTTGCAc                                                                                                                                      <  8:372758/147‑1 (MQ=255)
          gTGCAGCTTCTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCAc                                                                                                                                      <  6:325880/149‑1 (MQ=255)
               gCTTCTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCAccgccg                                                                                                                                 >  3:348961/1‑149 (MQ=255)
                   cTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGgc                                                                                                                               <  1:157481/147‑1 (MQ=255)
                    tGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGgcg                                                                                                                              >  5:363813/1‑147 (MQ=255)
                      cccGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAg                                                                                                                           >  1:156702/1‑148 (MQ=255)
                      cccGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAg                                                                                                                           >  1:161376/1‑148 (MQ=255)
                        cGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGACTGAAGTTGCACCGCCGGCGCAg                                                                                                                           >  5:44067/1‑146 (MQ=255)
                         gCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAg                                                                                                                           >  4:48789/1‑145 (MQ=255)
                                         cccGGGGTGTAGTTGCTTTGCACTTAGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGt                                                                                                       <  2:368369/149‑1 (MQ=255)
                                             gggTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAgg                                                                                                   <  5:182751/149‑1 (MQ=255)
                                                  tAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCt                                                                                               <  2:161376/148‑1 (MQ=255)
                                                     ttgcgttgcACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCAGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTc                                                                                           <  3:48789/149‑1 (MQ=255)
                                                       gcgttgcACTTCGTAAAGGATATCGCGGGACACCGCACGGACGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTcc                                                                                          <  6:188219/148‑1 (MQ=255)
                                                        cgttgcACATCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCt                                                                                         <  2:145538/148‑1 (MQ=255)
                                                         gttgcACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTgg                                                                                       >  1:89639/1‑149 (MQ=255)
                                                         gttgcACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTgg                                                                                       <  4:348961/149‑1 (MQ=255)
                                                                    tAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGAt                                                                            >  5:15294/1‑149 (MQ=255)
                                                                    tAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGAt                                                                            >  5:168436/1‑149 (MQ=255)
                                                                            atCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGACTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCggg                                                                     >  3:322416/1‑148 (MQ=255)
                                                                             tCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCggg                                                                     >  8:195897/1‑147 (MQ=255)
                                                                                  ggACACCGCACGGGCTACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGtt                                                              <  6:168436/149‑1 (MQ=255)
                                                                                   gACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTc                                                             <  6:363813/149‑1 (MQ=255)
                                                                                    acacCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGAGCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCg                                                            <  4:211363/149‑1 (MQ=255)
                                                                                        ctcACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGAGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTcc                                                                                          <  2:203479/113‑1 (MQ=255)
                                                                                        cGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCa                                                         >  4:103204/1‑148 (MQ=255)
                                                                                          cACGGGCGACGTTTGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAg                                                      <  2:368011/149‑1 (MQ=255)
                                                                                          cACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAg                                                      <  3:103204/149‑1 (MQ=255)
                                                                                               gCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGtt                                                 <  3:355472/149‑1 (MQ=255)
                                                                                                   cGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTg                                                >  7:172048/1‑146 (MQ=255)
                                                                                                   cGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGACTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTgaag                                             <  6:44067/149‑1 (MQ=255)
                                                                                                         cACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTGAAGAAgcgc                                       <  7:195897/149‑1 (MQ=255)
                                                                                                                      ttCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTGAAGAAGCGCCAGTAGGTGGTCg                          >  1:191704/1‑149 (MQ=255)
                                                                                                                           tCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTGAAGAAGCGCCAGTAGGTGGTCGCTTcc                     <  6:15294/149‑1 (MQ=255)
                                                                                                                              ggTAGATGTACAGGTCGCAGACTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTGAAGAAGCGCCAGTAGGTGGTCGCTTCCTTg                  <  4:322416/149‑1 (MQ=255)
                                                                                                                               gTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGTTCTGCGGGAAGCGTTCGGCAAAGCTGTTGAAGAAGCGCCAGTAGGTGGTCGCTTCCTTGc                 >  2:262938/1‑149 (MQ=255)
                                                                                                                               gTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTGAAGAAGCGCCAGTAGGTGGTCGCTTCCTTGc                 >  8:253670/1‑149 (MQ=255)
                                                                                                                                              gCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTGAAGAAGCGCCAGTAGGTGGTCGCTTCCTTGCCATCGAGCAGGCCgg  <  2:191704/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCAGGTCCTGGTGCAGCTTCTGCCCGCCAAAGCCCATGCGCCCCGGGGTGTAGTTGCGTTGCACTTCGTAAAGGATATCGCGGGACACCGCACGGGCGACGTTGGCACCGCAGAAGCGTTCGATCAGGTAGATGTACAGGTCGCAGGCTGAAGTTGCACCGCCGGCGCAGTAGATATTGTCGGCATCGGTCAGGTGCTTGTCCTGGTTCAGGCGGATCTGCGGGAAGCGTTCGGCAAAGCTGTTGAAGAAGCGCCAGTAGGTGGTCGCTTCCTTGCCATCGAGCAGGCCGG  >  NC_002947/1501108‑1501398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑