Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,506,934	C→T	7.2%	intergenic (+55/‑62)	petC → / → sspA	ubiquinol‑‑cytochrome c reductase cytochrome c1 subunit/stringent starvation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,506,934	0	C	T	7.2%	203.1 / 3.4	70	intergenic (+55/‑62)	petC/sspA	ubiquinol‑‑cytochrome c reductase cytochrome c1 subunit/stringent starvation protein A
Reads supporting (aligned to +/- strand):  ref base C (34/30);  new base T (3/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CAGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCGAGAAGGGC  >  NC_002947/1506787‑1507082                                                                                                                                                      |                                                                                                                                                      cAGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCa                                                                                                                                                      <  5:378805/149‑1 (MQ=255)  aGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTGGCA‑ATACTGCTAg                                                                                                                                                     <  6:215040/149‑1 (MQ=255)  aGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAg                                                                                                                                                     <  5:195874/149‑1 (MQ=255)        tCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTGGCA‑ATACTGCTAGGC‑CAcc                                                                                                                                              <  1:253230/149‑1 (MQ=255)            cACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCgg                                                                                                                                          <  1:360484/149‑1 (MQ=255)               cTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAGTACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGACAGGC‑CACCCCGGgcg                                                                                                                                       >  5:152048/1‑149 (MQ=255)               cTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCCGGGTG‑GCCGAGCA‑GTAATGCCCGGC‑CACCCCGGgcg                                                                                                                                       >  2:111238/1‑149 (MQ=255)                tATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGgcgc                                                                                                                                      >  6:127142/1‑149 (MQ=255)                tATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTAATTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATAACGAAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGACAGGC‑AACCCCGGgcgc                                                                                                                                      >  1:24150/1‑149 (MQ=255)                     gtTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCCGGGTT‑GGCGAGCACTTTTTGCACGGC‑CCCCCCGGgcggggtt                                                                                                                                  >  3:3375/1‑144 (MQ=255)                      ttGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGttca                                                                                                                                 <  2:50826/148‑3 (MQ=255)                              tCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCTCCCGTGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTaaa                                                                                                                         <  8:274725/148‑1 (MQ=255)                              tCTGGCTTTCTTCTTCGTATTCCCCTACTTGCTCACGCGTTAATCCTGCACGGACGTGCACTGATCCCGCAGTAGTTGCTGGTTGTTGAACGCGCCCGGGGGG‑GCCTAGCC‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTaaa                                                                                                                         <  5:262705/148‑1 (MQ=255)                                 ggCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTTCCAGGC‑CACCCCGGGCGCGTTTTCAATTAAAGTTg                                                                                                                     >  1:208987/1‑149 (MQ=255)                                  gCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTGTGCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTaaa                                                                                                                         <  1:279591/145‑1 (MQ=255)                                        ttcttcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAg                                                                                                               >  3:84409/1‑148 (MQ=255)                                          cttcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGc                                                                                                              <  5:127142/147‑1 (MQ=255)                                          cttcGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCAt                                                                                                            >  3:142329/1‑149 (MQ=255)                                             cGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATcc                                                                                                          <  4:84409/148‑1 (MQ=255)                                             cGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCg                                                                                                         >  5:214864/1‑149 (MQ=255)                                                aTTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CCCCCCGGGCGCGGTTTCATTTAAAGTTGCACAAGCATACGTgg                                                                                                      >  1:148920/1‑148 (MQ=255)                                                         cTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGACCGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCg                                                                                             <  8:343339/149‑1 (MQ=255)                                                             cTCAAGGTTGAATAGTGGAAGTACTTGCACTTTTCACTCAGTAGTTTCTGGTTGTTCAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTa                                                                                          <  2:208987/148‑1 (MQ=38)                                                             cTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGGG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTa                                                                                          <  5:296644/148‑1 (MQ=255)                                                                 agggttaaTAGTGGATGGAGGTGCAGTCATCACGCAGTAGTTTGTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATgg                                                                                     <  2:148920/143‑1 (MQ=255)                                                                   ggggacgaCTGGACGGCCGTGCACTGATCCCGCAGTAGTTGCGGGGTGGGGAACGCGCCCGGGGTG‑GCCTAGCA‑GTAGTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGgc                                                                                   <  8:205833/142‑1 (MQ=37)                                                                   cGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGgc                                                                                   <  6:214864/149‑1 (MQ=255)                                                                        aTACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGcaac                                                                              >  3:261812/1‑149 (MQ=255)                                                                           cTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGcaaccaa                                                                           >  2:57683/1‑149 (MQ=255)                                                                           cTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGcaaccaa                                                                           <  1:111238/149‑1 (MQ=255)                                                                              gAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGACCCTACATGGGCGCAACCAACAg                                                                        >  3:270485/1‑149 (MQ=255)                                                                               aaGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAgg                                                                       <  5:14496/149‑1 (MQ=255)                                                                                 ggACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGt                                                                      >  7:50051/1‑148 (MQ=255)                                                                                  gACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTGAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTa                                                                    <  3:211772/149‑1 (MQ=255)                                                                                  gACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGt                                                                      >  3:279982/1‑147 (MQ=255)                                                                                   aCGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAg                                                                   >  7:112204/1‑149 (MQ=255)                                                                                     gTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGcc                                                                 >  3:76888/1‑149 (MQ=255)                                                                                            gATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTAt                                                           >  1:4374/1‑148 (MQ=255)                                                                                             aTCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTc                                                         <  8:325815/149‑1 (MQ=255)                                                                                             aTCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTc                                                         <  8:50051/149‑1 (MQ=255)                                                                                              tCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTcc                                                        >  3:84920/1‑149 (MQ=255)                                                                                               cACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCGCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTcc                                                        <  4:84920/148‑1 (MQ=255)                                                                                                aCGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGa                                                      >  1:308073/1‑149 (MQ=255)                                                                                                 cGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGAt                                                     <  1:57683/149‑1 (MQ=255)                                                                                                  gCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATc                                                    >  2:372207/1‑149 (MQ=255)                                                                                                  gCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATc                                                    <  2:4374/149‑1 (MQ=255)                                                                                                    agtagtTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATccc                                                  >  4:153047/1‑149 (MQ=255)                                                                                                    agtagtTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGGTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATccc                                                  >  1:200454/1‑149 (MQ=255)                                                                                                           tCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGAt                                            >  2:146329/1‑148 (MQ=255)                                                                                                                ttgttgAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTAt                                      >  5:274869/1‑149 (MQ=255)                                                                                                                    tgAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATtct                                   >  8:7141/1‑148 (MQ=255)                                                                                                                       aCGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTTCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATc                               >  4:306560/1‑149 (MQ=255)                                                                                                                           gcCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCACGCTGATCACGATTATCATCGGGt                           >  6:128274/1‑149 (MQ=255)                                                                                                                            cccGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGtt                          >  3:31429/1‑149 (MQ=255)                                                                                                                            cccGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGtt                          <  8:112204/149‑1 (MQ=255)                                                                                                                             ccGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCTCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTc                         <  4:142329/149‑1 (MQ=255)                                                                                                                             ccGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTACGATCCCGCTGATCACTATTCTCATCGGGTTc                         >  2:237784/1‑149 (MQ=255)                                                                                                                                 ggTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCt                     <  7:7141/149‑1 (MQ=255)                                                                                                                                   tG‑GCCTAGCA‑GTCTTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTgg                   <  8:294324/149‑1 (MQ=255)                                                                                                                                   tG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCg                                                                                             <  2:22022/75‑1 (MQ=255)                                                                                                                                   tG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCg                                                                                             >  1:22022/1‑75 (MQ=255)                                                                                                                                   tG‑GCCTAGCA‑GTAGTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTgg                   <  2:24150/149‑1 (MQ=255)                                                                                                                                    g‑gCCTGGCA‑ATACTGCTAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATGACTATTATCATCGGGTTCGCCTGGt                  >  3:258192/1‑149 (MQ=39)                                                                                                                                    g‑gCCTGGCA‑ATACTGCTAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGt                  >  3:253219/1‑149 (MQ=255)                                                                                                                                        ctggCA‑ATACTGCTAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCt               >  2:91061/4‑149 (MQ=255)                                                                                                                                           gCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCAGTATTCTCATCGGGTTCGCCTGGTTCTGGc            >  8:252168/1‑149 (MQ=255)                                                                                                                                               gTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCga         <  1:372207/149‑1 (MQ=255)                                                                                                                                                  tcgCCAGGCGCACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCgag        >  7:68794/3‑148 (MQ=255)                                                                                                                                                    gCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCGAGAAgg    >  3:187861/1‑149 (MQ=255)                                                                                                                                                    gCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCGAGAAgg    >  8:139303/1‑149 (MQ=255)                                                                                                                                                      cAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCGAGAAGGGc  <  2:308073/149‑1 (MQ=255)                                                                                                                                                      cAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCGAGAAGGGc  <  1:146329/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CAGCGCATCGGCACCTATGTGTTGCTGTATCTGGCTTTCTTCTTCGTATTCGCCTACTTGCTCAAGCGTGAATACTGGAAGGACGTGCACTGATCACGCAGTAGTTTCTGGTTGTTGAACGCGCCCGGGGTG‑GCCTAGCA‑GTATTGCCAGGC‑CACCCCGGGCGCGTTTTCATTTAAAGTTGCACAAGCATCCGTGCGAGGAGCCGCTACATGGGCGCAACCAACAGGTTAGCCTGCTATTCCGATCCCGCTGATCACTATTCTCATCGGGTTCGCCTGGTTCTGGCCGAGAAGGGC  >  NC_002947/1506787‑1507082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑