Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,562,169	T→G	6.7%	intergenic (‑79/‑90)	pctA ← / → PP_1372	methyl‑accepting chemotaxis protein PctA/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,562,169	0	T	G	6.7%	176.7 / 4.1	60	intergenic (‑79/‑90)	pctA/PP_1372	methyl‑accepting chemotaxis protein PctA/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/27);  new base G (2/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

TGAAGGCGAGTATCACGATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGCCAGCCAGTCGGG  >  NC_002947/1562021‑1562315                                                                                                                                                     |                                                                                                                                                    tGAAGGCGAGTATCACGATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGt                                                                                                                                                     >  6:387389/1‑149 (MQ=255)       cGAGTATCACGATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCttt                                                                                                                                              >  7:329138/1‑149 (MQ=255)            agcACGATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGG‑GACTTTTCgg                                                                                                                                          <  6:93597/146‑1 (MQ=255)               ccGATCAGGGAGGCCGCCAAAACCATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGCTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGt                                                                                                                                      <  8:210075/148‑1 (MQ=255)                 gATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCtttt                                                                                                                                             >  5:399290/1‑140 (MQ=255)                 gATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCtttt                                                                                                                                             <  6:399290/140‑1 (MQ=255)                         aTGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CACTTTTCGGCTGTTCCTCTCTAt                                                                                                                            >  5:35305/1‑149 (MQ=255)                         aTGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGAAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTAt                                                                                                                            >  3:314120/1‑149 (MQ=255)                             cgccAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAg                                                                                                                        <  5:286833/149‑1 (MQ=255)                                  aaaGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGaa                                                                                                                   <  2:93123/149‑1 (MQ=255)                                               gCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGG‑GACTTTTCggg                                                                                                                                         <  4:225154/114‑2 (MQ=255)                                               gCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGG‑GACTTTTCggg                                                                                                                                         >  3:225154/1‑113 (MQ=255)                                                 tGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTa                                                                                                    >  7:357821/1‑149 (MQ=255)                                                 tGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTa                                                                                                    >  4:105874/1‑149 (MQ=255)                                                       gAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTa                                                                                                                             <  2:307695/118‑1 (MQ=255)                                                        aaGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGcc                                                                                              >  7:217418/1‑148 (MQ=255)                                                          ggCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGaa                                                                                           >  1:183804/1‑149 (MQ=255)                                                            cTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAAtt                                                                                         <  8:357821/149‑1 (MQ=255)                                                             ttttGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAAtt                                                                                         >  2:74022/1‑148 (MQ=255)                                                               ttGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTaa                                                                                       <  3:56170/148‑1 (MQ=255)                                                                   tCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTa                                                                                   >  2:112611/1‑148 (MQ=255)                                                                    cATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAGAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTa                                                                                   >  5:275677/1‑147 (MQ=255)                                                                     aTGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAg                                                                                >  5:220765/1‑149 (MQ=255)                                                                      tGGCGTAATCGGTTCCGCTAAGGTTTTAAATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAAAATCCAGAAAAGA‑CACTTTTCGGCTGTTCATCTCTATTAAGGCGAATAACACCAACAGGTATAGGACGAATTAAGCTAAAGc                                                                               >  8:162899/1‑149 (MQ=255)                                                                       ggCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCt                                                                              >  2:284054/1‑149 (MQ=255)                                                                        gCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCtt                                                                             >  4:266884/1‑149 (MQ=255)                                                                          gTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGa                                                                           <  2:183804/149‑1 (MQ=255)                                                                           tAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCtt                                                                             >  7:18178/1‑146 (MQ=255)                                                                                ggTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCaga                                                                      <  3:105874/148‑1 (MQ=255)                                                                                  ttCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGa                                                                   <  2:18869/149‑1 (MQ=255)                                                                                      gCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTa                                                                >  3:249407/1‑148 (MQ=255)                                                                                       cTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTAGCCTTTTAGGCTTTTGCTATATTTTAAGGCGAATAACACCCACAGGTATGGGCCGAATTAAGGTTAAGCTTGAGCAGAGGAttt                                                                >  1:116906/1‑147 (MQ=255)                                                                                       cTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTaaa                                                              <  1:74022/149‑1 (MQ=255)                                                                                       cTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTaaa                                                              <  6:275677/149‑1 (MQ=255)                                                                                        tAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTa                                                                >  2:303749/1‑146 (MQ=255)                                                                                          atggtttATATCAGGCTTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAaga                                                            <  8:358924/144‑1 (MQ=255)                                                                                          aGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAagat                                                           <  6:220765/149‑2 (MQ=255)                                                                                          aGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAagag                                                           <  8:217418/149‑1 (MQ=255)                                                                                             ttttATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTaaa                                                              <  6:359495/143‑1 (MQ=255)                                                                                             ttttATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTaaa                                                              >  5:359495/1‑143 (MQ=255)                                                                                                 atatCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCg                                                     >  6:351325/1‑148 (MQ=255)                                                                                                 atatCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCg                                                     <  4:179160/148‑1 (MQ=255)                                                                                                                aCGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCg                                     >  7:157919/1‑149 (MQ=255)                                                                                                                aCGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCg                                     >  4:389635/1‑149 (MQ=255)                                                                                                                 cGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGt                                    <  5:387389/149‑1 (MQ=255)                                                                                                                  gAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGtt                                   <  2:116906/149‑1 (MQ=255)                                                                                                                  gAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGtt                                   <  8:157919/149‑1 (MQ=255)                                                                                                                   aGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTg                                  >  7:232665/1‑149 (MQ=255)                                                                                                                       tatccttAGGTTTTGCAACATGCCGATAAGT‑CGCTTTTCGGCTTTTCCTCTCTATTAAGGCGAATAACACGCTCAGGTTTAGTCCTAATTAAGCTAATGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAg                               <  7:162899/142‑1 (MQ=255)                                                                                                                             tAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCggg                        >  2:228514/1‑149 (MQ=255)                                                                                                                                gTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGccc                     <  1:228514/149‑1 (MQ=255)                                                                                                                                    tgCAACATCCCGAAAACT‑CCCTTTTCGGCTGATCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGAt                 <  8:329138/149‑1 (MQ=255)                                                                                                                                         cATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGgcca            <  1:303749/149‑1 (MQ=255)                                                                                                                                            gccGAAAAGG‑GACTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGgccagc          >  8:19939/2‑148 (MQ=255)                                                                                                                                            cccGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGgccagc          >  4:56936/1‑148 (MQ=255)                                                                                                                                             ccGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGgccagc          >  6:39813/1‑147 (MQ=255)                                                                                                                                              cGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGccagccag       >  3:238893/1‑149 (MQ=255)                                                                                                                                                aaaaGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGCCAGCCAGTc     <  5:351325/149‑1 (MQ=255)                                                                                                                                                aaaaGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGCCAGCCAGTc     <  2:246126/149‑1 (MQ=255)                                                                                                                                                  aaGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGTCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGCCAGCCAGTCgg   <  4:249407/149‑1 (MQ=255)                                                                                                                                                   act‑CCCTTTTCGGCTCTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCTGCCGAATTAAGCTAAAGCATGAGCAGAGCATTAAAGAGATGACGGAATTTTCGACCAACGTTGTAGGCGAGGGCACGGATGGCCAGCCAGTCagg  >  3:335100/3‑149 (MQ=255)                                                                                                                                                   aGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCACGGATGGCCAGCCAGTCggg  >  3:340550/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TGAAGGCGAGTATCACGATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGT‑CCCTTTTCGGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGCCAGCCAGTCGGG  >  NC_002947/1562021‑1562315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑